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The Effect of NaOH treatment-Citric acid neutralization of Korean Standard Operating Procedure (SOP) on Physicochemical Compositions of Domestic Swine Excrement for the Connection to Thermophilic Aerobic Oxidation (TAO) (구제역 긴급행동지침(SOP)상의 화학처리방법으로 처리된 양돈분뇨의 고온호기산화공정 적용 연구)

  • Kim, Ha-Je;Kim, Soo-Ryang;Hong, In-Gi;Jeon, Sang-Jun;Kim, Cho-Rong;Jung, Gwang-Hwa;Han, Ho;Kim, Dong-Kyun;Lee, Myung-Gyu
    • Journal of Animal Environmental Science
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    • v.19 no.2
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    • pp.81-88
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    • 2013
  • We planned to study how the chemical disposal designed by SOP can affect on physicochemical compositions of the livestock excrement. According to Livestock Manure Management Scheme, we experimented in two steps; the first step, NaOH treatment-Citric acid neutralization, and then the second procedure, Thermophilic Aerobic Oxidation (TAO) system. Physicochemical compositions of the 3-days-old samples after NaOH treatment were pH 10.31, EC 24.54 mS/cm, SCOD 3,022 mg/L, T-N 4,315 mg/L, $NH{_4}^+-N$ 1,960 mg/L, and not detected E. coli.. And those of one-day-old samples after citric acid neutralization were pH 7.36, EC 32.89 mS/cm, $SCOD_{Mn}$ 12,733 mg/L, T-N 4,787 mg/L, $NH{_4}^+-N$ 2,450 mg/L, and E. coli. not detected. In contrast, the physicochemical compositions of the treatment plots after the second treatment with TAO system (72hr) were pH 9.42 EC 24.21 mS/cm, $SCOD_{Mn}$ 3,660 mg/L, T-N 3,616 mg/L, $NH{_4}^+-N$ 1,190 mg/L, and no detection of E. coli.

Analysis of Sludges from Wastewater of Hanji Production (한지 생산공정에서 발생하는 슬러지의 분석)

  • Choi, Hee-Seon;Kim, Tai-Jin
    • Analytical Science and Technology
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    • v.12 no.4
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    • pp.318-325
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    • 1999
  • Sludges from the wastewater of hanji production were analysed to see that sludges from the bleaching process, the bleach washing process, and the paper preparation process were fibrous, while sludges from the process cooking with NaOH and the process of final chemical and microbiological treatment were non-fibrous. Sludge solution from the bleaching process had the most amount of solid matters. Sludges from the bleaching process, the bleach washing process, and the final chemical and microbiological treatment process contained about 80-90 %(w/w) moisture. Owing to hypochlorite ion, pH of sludge from the bleaching process was higher than any other sludge samples. The inorganic components of sludges were determined by ICP-AES. The major inorganic component of sludge from the process cooking with NaOH was Na, while the sludge from the bleaching process had Na and Ca, major components in the final chemical and microbiological treatment process were Ca and Al. Trace amount of Mg, Fe, K, P, Mn and Ti were observed in sludges. Sludge from the bleaching process had ashes more than any other sludges. Shape of sludges observed by SEM was appeared to fibrous or crystalline, and the breadth of fibrous sludge was $5{\sim}50{\mu}m$. The sludge from the bleaching process had the most amount of matters soluble by cold and hot water and by alcohol-benzene.

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Geochemical Behavior, Dispersion and Enrichment of Environmental Toxic Elements in Coaly Metapelites and Stream Sediments at the Hoenam Area, up the Taecheong Lake, Korea (대청호 상류, 회남일대에 분포하는 탄질 변성니질암과 하상 퇴적물의 환경유해원소에 관한 지화학적 거동, 분산 및 부화)

  • Lee, Chan Hee;Lee, Hyun Koo
    • Economic and Environmental Geology
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    • v.30 no.3
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    • pp.209-222
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    • 1997
  • The Hoenam area, up the Taecheong lake, composed mainly of low grade coal-bearing metapelites within the Ogcheon Supergroup. These coal formations are developed discontinuously several hundred meters and swelling from 10 to 300 cm along the host metapelites. Although the formations have been mined for coal, but already mined out, and the formations were higher content (mean value of 42 samples) of environmental toxic elements as As (13 ppm). Ba (1.81 wt. %), Cd (2 ppm), Cr (188 ppm), Cu (87 ppm), Mo (214 ppm), Pb (25 ppm), Sb (3 ppm), Se (12 ppm), U (55 ppm), V (2124 ppm) and Zn (234 ppm) than the host metapelites and the NASC. The Al, Ti, Mg, K and Na contents in stream sediments derived from the Hando and Bugook mine area were highly concentrations than the samples from the Samseongjeil mine area. The mean value (wt. %) of Fe (10.07), Mn (0.15), Ca (0.84), P (0.18) and Ba (0.77) influenced by the Samseongjeil mine were higher than the other mine drainage sediments. The mean content (ppm) of environmental toxic elements in drainage sediments from the Samseongjeil mine were taken As (2083), Cu (447), Mo (202), Ni (720), Pb (42), U (250), V (1070) and Zn (2632), which are extremely high concentrations against NASC and EPA. Characteristics of elemental behavior and dispersion of the all toxic elements are the same as increased with increasing U, V, and Cu. Rare earth elements in the sediments are enriched with LREE (La, Ce and Nd) from the drainage on strong concentration of toxic elements. The pH of stream water is neutral, but pH of the sediments ranged from 4.92 to 6.93 (mean 6.22), those are slightly acid in the Hando mine area. Major elements in the host rocks at the Hoenam area are mostly depleted especially Ca, excepting Ti and Ba, normalized with NASC. The sediments were highly enriched of Ti, Fe, Mg, Mn and Ba, but depleted of Al, K, Ca, Na and P on the basis of host rocks and NASC. Minor and environmental toxic elements in the host rocks were strongly enriched all elements (As, Cd, Mo, Se, D, V and Zn), excepting Co, Ni and Sr. Enrichment index (mean value) about toxic elements (As, Cr, Cu, Fe, Ni, Pb and Zn) of the sediments in this area have taken 41.35 (Hando mine drainage; 2.73, Samseongjeil mine drainage; 113.14 and Bugook mine drainage; 8.19), those are seriously contaminated by environmental toxic elements.

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An Evaluation of the Student Performance and Perception of the Food and Nutrition Subject in the Elementary School Curriculum in Incheon, Korea (실과의 식품영양 단원에 관한 교사 학생의 인지 및 학생의 지식, 태도, 기능간의 상관 연구 -인천직할시 공립국민학교를 중심으로-)

  • 김수자
    • Journal of Korean Home Economics Education Association
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    • v.5 no.1
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    • pp.97-111
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    • 1993
  • 본 연구는 국민학교 실과 과목의 식품 영양 단원에 관한 교사, 학생의 인지 및 학생의 지식, 태도, 기능 평가를 위하여 71개 인천시내 공립 국민학교 4,5,6학년 교사 510명과 12개교학생 1,754명을 대상으로 설문조사를 통한 내용을 분석한 것으로 연구의 목적은 1)실과의 정해진 교육목표에 관한 교사의 인지정도 규명 2)식품, 영양 단원에서 교사가 중요하다고 인지하는 내용과 가르치기에 어려움을 느끼는 내용 규명 3)교사가 인지하는 식품영양 단원학습을 증진하기 위한 요소 4) 학생들이 흥미를 느끼는 내용 5)본 단원 학습 후 학생의 지식, 태도, 기능 정도 6)지식, 태도 기능간의 상호 상관관계 규명 등이며 그 연구결과는 다음과 같다. 1. 교사들이 인지하는 실과의 정해진 교육목표는 거의 모두가 중요한 것으로 나타났다. 2. 교사들이 인지하는 식품 영양 단원의 중요한 내용은 영양과 질병과의 관계, 식품위생, 식품저장 등이며, 가장 중요하지 않은 내용으로는 영양 필요량이었다. 또한, 지도에 어려움을 느끼는 내용은 영양 필요량, 조리준비, 식품구성/함유량 등이며 어려움을 느끼지 않는 내용은 영양과 질병과의 관계, 식품위생, 식품저장 등이었다. 3. 교사가 인지하는 단원 학습 증진을 위한 긍정적 요소는 적합한 실습설비와 자료, 시청가자료등이었고 부정적 요소는 남교사, 실험 실습이 필요한 부분은 숙제로 미루는 것 등이었다. 4. 학생들이 흥미를 느끼는 내용으로는 간단한 음식 만들기, 영양소의 기능, 영양문제 등이었고, 쥬스나 차 준비, 조리연료, 부엌용구 사용법 등은 흥미를 느끼지 못하는 내용이었다. 5. 학생의 학업성취 중 지식은 4학년이 식품위생, 기초식품군이, 5학년은 알맞은 조리용구, 조리원리, 6학년은 여가활용의 뜻, 올바른 예절 등이 90% 이상 바르게 답하였고 5학년의 삶은 달걀의 화학적 변화, 감자의 주된 영양 등은 60%이하였다. 6 태도 테스트 결과는 5학년이 4,6학년보다 약간 높은정도(Mn=3.653) 였으나 기능테스트에서는 4학년이 5,6학년보다 약간 낮게 (Mn=3.0) 나타났다(구체적인 것은 본문 참조). 7, 4, 5, 6학년 모두 지식, 태도 기능 간에 정적 상관을 나타내었는데 특히 태도와 기능 사이에는 4학년 r=.7166, 5학년 r=.5175, 6학년 r=.6796을 나타내었고 지시과 태도(4학년 r=.1546, 5학년 r=.0872, 6학년 r=.2192)와 지식과 기능사이에서는 (4학년 r=.1631, 5학년 r=.0585, 6학년 r=.2521)태도와 기능간보다 약한 상관관계를 보였다.

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Improvement of Hydrogen Storage Properties of Mg by Addition of NbF5 via Mechanical Milling under H2

  • Kwak, Young Jun;Song, Jiyoung;Mumm, Daniel R.
    • Korean Journal of Materials Research
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    • v.23 no.10
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    • pp.562-567
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    • 2013
  • A 90 wt% Mg-10 wt% $NbF_5$ sample was prepared by mechanical milling under $H_2$ (reactive mechanical grinding). Its hydriding and dehydriding properties were then examined. Activation of the 90 wt% Mg-10 wt% $NbF_5$ sample was not required. At n=1, the sample absorbed 3.11 wt% H for 2.5 min, 3.55 wt% H for 5 min, 3.86 wt% H for 10 min, and 4.23 wt% H for 30 min at 593K under 12 bar $H_2$. At n=1, the sample desorbed 0.17 wt% H for 5 min, 0.74 wt% H for 10 min, 2.03 wt% H for 30 min, and 2.81 wt% H for 60 min at 593K under 1.0 bar $H_2$. The XRD pattern of the 90 wt% Mg-10 wt% $NbF_5$ after reactive mechanical grinding showed Mg, ${\beta}-MgH_2$ and small amounts of ${\gamma}-MgH_2$, $NbH_2$, $MgF_2$ and $NbF_3$. The XRD pattern of the 90 wt% Mg-10 wt% $NbF_5$ dehydrided at n=3 revealed Mg, ${\beta}-MgH_2$, a small amount of MgO and very small amounts of $MgH_2$ and $NbH_2$. The 90 wt% Mg-10 wt% $NbF_5$ had a higher initial hydriding rate and a larger quantity of hydrogen absorbed for 60 min than the 90 wt% Mg-10 wt% MnO and the 90 wt% Mg-10 wt% $Fe_2O_3$, which were reported to have quite high hydriding rates and/or dehydriding rates. The 90 wt% Mg-10 wt% $NbF_5$ had a higher initial dehydriding rate (after an incubation period) and a larger quantity of hydrogen desorbed for 60 min than the 90 wt% Mg-10 wt% MnO and the 90 wt% Mg-10 wt% $Fe_2O_3$.

Preparation of Aliphatic Polyester by Lipase Catalyzed Transesterificatoin in Anhydrous Organic Solvents (유기용매에서 Lipase에 의한 지방족 폴리에스터의 합성)

  • 박현규;장호남
    • KSBB Journal
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    • v.9 no.3
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    • pp.246-252
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    • 1994
  • Enzyme-catalyzed polycondensatlon reaction of aliphatic polyesters with several repeating units was studied using the biocatalytic activities of lipases from different sources. Porcine pancreatic lipase (PPL) was found to be best in utilizing bls(2,2,2-trichloroethyl) glutarate and 1,4-butanediol as substrafes. The reaction was also catalyzed to some extent by the lipases from Humicola lanuginos and Psudomonas sp. In the series of short-chain diols(C2-C4), bis(2,2,2-trichloroethyl) glutarate was iransesterified fastest with 1,4-butanediol and for the long-chain diols (PEG-300-PEG-1000), the reaction was fastest with PEG-400. With PEGs, only monoesterification product was obtained. PPL functioned well in relatively hydrophilic organic solvents such as tetrahydrofuran(THF), ether and acetonitrile. The reaction rate was accelerated as the reaction temperature was raised from $20^{\circ}C$ to $60^{\circ}C$ while Mn values of the reaction products were not affected by the reaction temperature. End group analysis by NMR showed that Mn values of the polymer were in the range of 1500-4000 daltons.

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Characterization of a Fibrinolytic Enzyme Produced by Bacillus subtilis MJ-226 Isolated from Meju (전통 메주에서 분리한 Bacillus subtilis MJ-226이 생산하는 혈전용해효소의 특성)

  • Lim, Sung-Mee
    • Korean Journal of Microbiology
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    • v.45 no.4
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    • pp.377-384
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    • 2009
  • Among 27 Bacillus sp. isolated from Meju, a traditional Korean soybean fermented food, a strain MJ-226 was selected due to its strong fibrinolytic activity, and it was identified to be Bacillus subtilis MJ-226 according to morphological and biochemical characterization and sugar utilization. The fibrinolytic enzyme of B. subtilis MJ-226 was maximally produced by cultivating in the Tryptic Soy Broth (TSB) for 24~26 h at $37^{\circ}C$, and the enzymes activity was promoted with adding glucose, fructose, peptone or yeast extract to TSB. The fibrinolytic enzyme was stable at the range of pH from 6.0 to 8.0, and between 35 and $40^{\circ}C$. Also, when the crude enzyme was exposed to various metal ions and chemical inhibitors for 12 h, the enzyme stability was maintained by $MnSO_4$, $CaCl_2$, KCl, and NaCl. However, the stability was destroyed by treatment with $CuSO_4$, $MgSO_4$, $ZnSO_4$, $FeSO_4$, and $BaCl_2$, and the enzyme was unstable in the presence of chemical inhibitors such as iodoacetic acid, leupeptin, phenylmethanesulphonyl fluoride (PMSF), sodium dodecyl sulfate (SDS), thiourea, trans-1,2-diaminocyclohexane-N,N,N',N'-tetraacetic acid (CDTA) and ethylenediaminetetraacetic acid (EDTA).

IN VIVO CHANGES OF THE ACIDITY OF VARIOUS DENTAL LINERS (In vivo에서 수종이장재의 산도변화에 관한 연구)

  • Lee, Mi-Jeong;Ahn, Yun-Sil;Lee, Yong-Woo;Son, Ho-Hyun;Lee, Kwang-Won
    • Restorative Dentistry and Endodontics
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    • v.23 no.1
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    • pp.433-442
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    • 1998
  • The purpose of this study was to observe the changes of acidity of resin cement(Time Line), glass ionomer cement(GC Fugi Lining LC), zinc phosphate cement(Fleck's zinc cement). zinc oxide eugenol cement(Sultan,Chemists.) in vivo and in vitro. Class I cavities with 3mm depth were prepared on the occlusal surfaces of 20 recently extracted human Mn. molar teeth and 20 human Mn. 3rd molar teeth in oral cavity. The prepared cavities were divided into 4 groups of each 5 teeth using the above 4 cavity liners. Each cement was mixed in accordance with manufacturer's direction at the room temperature of $23^{\circ}{\pm}5^{\circ}C$ and filled into the cavity in a width of 1 mm. The microelectrode of pH meter was inserted into the prepared cavity which was filled with mixed cement, and the acidity of cement was measured for 3 days from the beginning of cement mix in vitro and in vivo. The measured acidity was then statistically analyzed by ANOVA. The results were as follows. 1. In vitro, the pH of zinc oxide eugenol cement was statistically lower than that of the three other groups at 2min, 4min, 6min, 8min, 10min, 12min, 18min, 20min. (p<0.05). 2. The pH of zinc oxide eugenol cement in vivo was statistically higher than that in vitro at 16min,16min, 20min(p<0.05). 3. The pH of zinc phosphate cement in vivo was statistically higher than that in vitro at 4min, 20min(p<0.05). 4. In vitro and in vivo, there was no significant difference in the pH between the resin cement and the glass ionomer cement(p>0.05). 5. The initial acidity was not high, but almost neutral in all kinds of the cements.

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Enzymatic Characteristics of Polyphenol Oxidase from Apple (Ralls Janet) (사과(Ralls Janet) Polyphenol Oxidase의 효소학적(酵素學的) 성질(性質))

  • Chung, Ki Taek;Seu, Seung Kyo;Song, Hyung Ik
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.12 no.4
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    • pp.316-322
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    • 1983
  • In order to obtain elementary data of enzymatic browning of apples and apple products and to examine effectively inhibitory method of browning, we extracted polyphenol oxidase (EC 1.10.3.1) from apple (Ralls Janet) and investigated its general properties. The optimum conditions for the enzyme reaction were pH 6.0 and temperature of $30^{\circ}C$. The enzyme was very stable at pH 4.0, and at the range of pH 5.0-9.0 its activity was above 80% compared with pH 4.0. The enzyme was very stable by heating at $40^{\circ}C$ for 1 hour, and almost 50% of enzyme activity was lost by heating at $60^{\circ}C$ for 30 minutes. The polyphenol oxidase activity was enhanced by the addition of $Cu^{2+}$ and $Mn^{2+}$, respectively, meanwhile $Na^+$, $Hg^{2+}$ and $Co^{2+}$ inhibited the enzyme activity. The enzyme activity was greatly decreased in the presence of inhibitors such as cysteine, sodium metabisulfite and ascorbic acid. The polyphenol oxidase greatly catalyzed the oxidation of o-diphenols such as chlorogenic acid and catechol, which suggests that main substrate of polyphenol oxidase is o-diphenol compounds.

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Runoff Characteristics of Non-point Source Pollutant Loads Generated on Golf Course (골프장에서 발생하는 비점오염원 유출특성)

  • Shin, Minhwan;Choi, Jaewan;Choi, Younghun;Park, Woonji;Won, Chulhee;Shin, Dongsuk;Lim, Kyoung Jae;Choi, Joongdae
    • Journal of Korean Society on Water Environment
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    • v.27 no.6
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    • pp.784-793
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    • 2011
  • Activities on golf courses are believed to contribute to the degradation of water quality in receiving waters due to the excessive use of farm chemicals including fertilizers and pesticides. The objective of this study was to collect basic data that could explain the characteristics of non-point source (NPS) pollution discharged from a golf course. Twenty seven water quality monitoring was conducted at a golf course during the rainy season of 2008 and 2009. The results indicated that the ranges of the Event Mean Concentration (EMC) at the golf course were $BOD_5$ 1.8~11.3 (ave. 5.6) mg/L, $COD_{Mn}$ 19.2~51.4 (ave. 39.6) mg/L, TOC 11.0~31.0 (ave. 16.8) mg/L, TN 1.545~16.098 (ave. 5.623) mg/L, TP 0.230~4.528 (ave. 1.525) mg/L, and SS 2.2~57.3 (ave. 10.1) mg/L. The unit loads of the golf course estimated were $BOD_5$ $3.35kg/km^2/day$, SS $6.43kg/km^2/day$, $COD_{Mn}$ $30.00kg/km^2/day$, TN $4.04kg/km^2/day$, TP $1.14kg/km^2/day$, and TOC $12.16kg/km^2/day$. Golf courses are currently classified as a grass field in which the unit loads are different from golf courses. Therefore, it was recommended that golf courses need to be separated from the grass field when the surveys and modelings for Total Maximum Daily Load (TMDL) development and the evaluation of TMDL implementation were performed.