• Title/Summary/Keyword: MlMO

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Serum Protein Values of Racehorse in Korea (한국경주마의 혈청단백질치)

  • Lee Chang-Woo;Lee Sung-Yub;Lee Sung-Mo;Eom Yeong-Ho
    • Journal of Veterinary Clinics
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    • v.4 no.2
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    • pp.445-448
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    • 1987
  • 임상적으로 건강하고 휴식상태에 있는 한국 경주마의 혈청단백질치를 조사하였다. 총단백질농도는 6.5$\pm$0.3g/100ml(평균$\pm$표준편차)이였다. 알부민 농도는 3.4$\pm$0.2, $\alpha$-글로부린 농도는 0.8$\pm$0.1, $\beta$-글로부린 농도는 1.2$\pm$0.2, ${\gamma}$-글로부린 농도는 1.1$\pm$0.3g/100ml이었으며, A/G 비는 1.15$\pm$0.21이었다. 성, 연령, 계절에 따르는 혈청단백질치의 차이를 통계적으로 분석하여 보고하였다.

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Spectrophotometric Determination of Ion Pair Extraction of Quaternary Amines with Methyl Orange (Methyl Orange에 의한 4급 Amine류의 Ion Pair 추출 흡광도 정량(I) - Cetyltrimenthylammonium bromide, Brethlium tosylate, Gallamine triethiodide 및 Thiamine의 정량)

  • 최종환;김영수
    • YAKHAK HOEJI
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    • v.31 no.2
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    • pp.45-51
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    • 1987
  • Methyl orange(MO) and cetyltrimethylammonium bromide(CTA) were mixed and added to 10ml of the buffer solution and then the solution was shaken for 5 minutes. The maximum absorption wavelength of the reaction product was 419nm. Dichloromethane was the best extracting solvent among the several organic solvents and the most suitable pH range was 2~8. When the CTA-MO calibration curve was made in the best experimental condition, the Lambert-Beer's law was obeyed in the range of CTA concentration of $1.5\times$$10^{-5}$~1.0$\times$$10^{-4}M$ by UV spectrophotometer. This method was possible to determine quaternary ammonium salts in the pharmaceutical preparations.

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A Study on Reforming Reaction for Preparation of Synthesis Gas from Land-Fill Gas (매립지가스(LFG)로부터 합성가스 제조를 위한 개질반응 연구)

  • Cho, Wooksang;Yoon, Jungsup;Park, Sunggyu;Mo, Yongki;Baek, Youngsoon
    • Journal of Hydrogen and New Energy
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    • v.25 no.6
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    • pp.570-576
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    • 2014
  • LFG (Land-Fill Gas) includes components of $CH_4$, $CO_2$, $O_2$, $N_2$, and water. The preparation of synthesis gas from LFG as a DME (Dimethyl Ether) feedstock was studied by methane reforming of $CO_2$, $O_2$ and steam over NiO-MgO-$CeO_2$/$Al_2O_3$ catalyst. Our experiments were performed to investigate the effects of methane conversion and syngas ratio on the amount of LFG components over NiO-MgO-$CeO_2$/$Al_2O_3$ catalyst. Results were obtained through the activity reaction experiments at the temperature of $900^{\circ}C$ and GHSV of 4,000. The results were as following; it has generally shown that methane conversion rate increased with the increase of oxygen and carbon dioxide amounts. Highly methane conversion of 92~93% and syngas ratio of approximately 1.0 were obtained in the feed of gas composition flow-rate of 243ml/min of $CH_4$, 241ml/min of $CO_2$, 195ml/min of $O_2$, 48ml/min of $N_2$, and 360ml/min of water, respectively, under reactor pressure of 15 bar for 50 hrs of reaction time. Also, it was shown that catalyst deactivation by coke formation was reduced by excessively adding oxygen and steam as an oxidizer of the methane reforming.

Effects of In Vitro Fertilization Conditions of In Vitro Matured Cumulus-Intact Pig Oocytes on Embryo Development

  • Kim, Jae-Young;Lee, Eun-Ji;Park, Jin-Mo;Lee, Hong-Cheol;Park, Hum-Dai;Kim, Jae-Myeong
    • Journal of Embryo Transfer
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    • v.26 no.2
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    • pp.117-122
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    • 2011
  • In this study, we examined the effectiveness of in vitro fertilization of porcine immature oocytes on the embryo development of blastocysts or hatched blastocysts and the number of cells according to the in vitro fertilization conditions. In the in vitro fertilization of in vitro matured porcine oocytes, there were no significant differences between treatment groups regarding fertilization rate, blastocyst rate, and embryo development of hatched blastocysts according to the storage periods of liquid sperm of 24, 48, and 72 hours. The embryo development rate of hatched blastocysts after the fertilization according to different spermatozoa concentrations ($0.4{\times}10^5$, $1.2{\times}10^5$, and $3.6{\times}10^5$ cells/ml) showed the highest rate in the group with a spermatozoa concentration of $1.2{\times}10^5$ cells/ml; in particular, this rate was significantly higher than that in the $0.4{\times}10^5$ cells/ml group (p<0.05). The total number of blastocysts cells as well as trophectoderms (TE) that developed in each treatment group were also significantly higher in the $1.2{\times}10^5$ cells/ml group than in any other groups (p<0.05). In contrast, the embryo development rate of blastocysts according to different co-incubation periods of sperm and oocyte (1, 3, and 6 hr) was high in the 6-hour group; in particular, the rate was significantly higher than that of the I-hour group (p<0.05). Furthermore, the total number of oocytes cells and TEs that developed was significantly higher in the 6-hour group than any other group (p<0.05). In this study, the most effective treatment conditions for porcine embryo development and high cell number were found to be as follows: a sperm storage period of less than 72 hours, a spermatozoa concentration of $1.2{\times}10^5$ cells/ml, and a 6-hour co-incubation period for sperm and ooocyte.

음악관련 전자상거래 시스템 개발

  • Lim, Hyeok;Jeong, Yeon-Mo;Oh, Jae-Gon;Lee, Gi-Chang
    • Proceedings of the Korea Society for Simulation Conference
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    • 2000.11a
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    • pp.204-207
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    • 2000
  • 본 논문에서는 기존 쇼핑몰의 상품 판매 기능을 이용해 음악 관련 컨텐트를 취급할 수 있는 전자상거래 시스템의 개발에 대해 제안하였다. ASP기반으로 동작하며, 일반적인 상품 판매 기능 이외에 악보를 검색, 판매할 수 있는 기능을 추가했다. 악보는 SMF(Standard Midi File)를 소스로 사용해 기존의 인쇄물 흑은 비트맵, 포스트 스크립트 타입의 악보에서는 불가능했던 여러 가지 기능들을 가지며, Music ML을 이용해 악보로 표현하도록 했다. 개발한 시스템을 실제로 사용하기 전에 시뮬레이션을 통해서 검증하였다.

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Mineral Contents in Bottled Natural Water and Estimation of Their Intake by Korean Adults (일부 무기질의 생수 중 함량 분석과 생수를 통한 섭취량 평가)

  • Kim, Moo-Kyung;Sim, Jin-Ah;Eom, Hee-Jung;Kim, Myung-Hee;Choi, Mi-Kyeong
    • Journal of the Korean Dietetic Association
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    • v.16 no.2
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    • pp.116-121
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    • 2010
  • Minerals play an important role in the body as essential nutrients. However, it is not easy to meet DRIs because food sources of minerals are limited. Recently, consumption of bottled natural water has been increasing in Korea due to water pollution and distrust of tap water. The present study was conducted to investigate mineral contents in bottled natural water and their intakes among Korean adults. We analyzed eight minerals in seven kinds of bottled natural water by ICP-spectrometry and conducted a survey on the intake status of water and bottled natural water with 400 Korean young adults. The mean contents of Ca, Mg, Fe, Zn, Cu, Mn, Se, and Mo in bottled natural water were 22.45${\pm}$22.48 mg/L, 10.59${\pm}$9.97 mg/L, 0.27${\pm}$0.18 ${\mu}g/L$, 2.06${\pm}$1.48 ${\mu}g/L$, 5.47${\pm}$0.70 ${\mu}g/L$, 1.43${\pm}$0.37 ${\mu}g/L$, 1.90${\pm}$0.96 ${\mu}g/L$, and 3.34${\pm}$0.79 ${\mu}g/L$, respectively. The mean age, height, weight, and BMI were 22.76 years, 174.94 cm, 68.64 kg, and 22.41 $kg/m^2$ for males (n=150) and 21.25 years, 162.04 cm, 51.05 kg, and 19.46 $kg/m^2$ for females (n=250), respectively. The respective daily intakes of total water and bottled natural water as water itself were 670.30 ml and 212.20 ml for males and 488.04 ml and 132.72 ml for females. The daily intakes of Ca, Mg, Fe, Zn, Cu, Mn, Se, and Mo from bottled natural water were 4.76 mg, 2.25 mg, 0.06 ${\mu}g$, 0.44 ${\mu}g$, 1.16 ${\mu}g$, 0.30 ${\mu}g$, 0.40 ${\mu}g$, and 0.71 ${\mu}g$ for males and 2.98 ${\mu}g$, 1.41 mg, 0.04 ${\mu}g$, 0.27 ${\mu}g$,0.73 ${\mu}g$, 0.19 ${\mu}g$,0.25 ${\mu}g$, and 0.44 ${\mu}g$ for females, respectively. Overall, the contents of Ca, Mg, and Se in bottled natural water were relatively high and the daily intakes of these minerals were 0.4~1.0% of the DRIs.

Quality Investigation of Commercial Northern Sand Lance, Ammodytes Personatus Sauces (시판 까나리액젓의 품질조사)

  • CHO Young Je;IM Yeong Sun;LEE Keun Woo;KIM Geon Bae;CHOI Young Joon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.32 no.5
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    • pp.612-617
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    • 1999
  • The quality characteristics of 15 kinds of the commercial northern sand lance, Ammodytes Personatus sauce (CNS) of korean traditional salt-fermented fish sauces were evaluated comparing to the traditional northern sand lance, Ammodytes Personatus sauce (TNS). The ranges of chemical compositions of the CNSs were $66.5\~71.0\%$ moisture, $19.3\~24.6\%$ ash, $4.7\~12.0\%$ crude protein, and the pH and salinity were $5.56\~6.47\%,\;24.0\~32.9\%$, respectively. Total nitrogen, amino nitrogen, total free amino acid and total ATP related compounds (sum of ATP$\~$IMP, HxR, Hx and uric acid) were in the ranges of 0.781$\~$ 1,918 g, 445.9$\~$1037.9 mg, 3,258.9$\~$6:562.6mg in 100 ml CNSs, and 4.766$\~$8.989 $\mu$mol in 1 ml CNSs. The CNSs were higher in content of moisture, TMAO, TMA and pH, but lower in content of crude protein, salinity, total nitrogen, amino nitrogen, total ATP related compounds, absorbance at 453 nm and total free amino acid than the TNSs, Both CNS and TNS samples were rich in free amino acids, such as glutamic acid, lysine, alanine, leucine, valine, aspartic acid and isoleucine in the order.

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The Effect of IGF-1 on ALP Activity of MC3T3-E1 Cell (MC3T3-E1세포의 ALP activity에 대한 IGF-I의 영향)

  • Lee, Hu-Jung;Lee, Jae-Mok;Choi, Byung-Ju;Yu, Hyun-Mo;Shu, Jo-Young
    • Journal of Periodontal and Implant Science
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    • v.27 no.4
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    • pp.669-684
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    • 1997
  • Polypeptide growth factors belong to a class of potent biologic mediators which regulate cell differentiation, proliferation, migration and metabolism. IGF-I is polypeptides secreted by skeletal cells and is considered as regulators of bone formation. The purpose of this study is to evaluate the effects of IGF-I on bone nodule formation and alkaline phosphatase activity of MC3T3-E1 cells. MC3T3-E1 cells were seeded at $1{\times}10^4$ cells/well, $1{\times}10^5$ cells/well in alpha-modified Eagle medium containing 10% fetal bovine serum, 10 mM ${\beta}-glycerophosphate$ and $5O{\mu}g/ml$ of ascorbic acid. Before 48 hours of indicated time, medium were changed with serum free medium. After 24 hours, 0.1, 1, 10 ng/ml IGF-I were added to the cells and cultured for 3, 7, 14, 21, 28 days. And histochemical analysis was done and ALP activity was measured and was expressed as nmol/min/mg of protein. The bone nodule formation in MC3T3-E1 cells of IGF-I was seen at 21, 28 days, but there were no difference between control group and experimental groups. The ALP activity decreased when it is compare to control 2 group except for 1 ng/ml, 10 ng/ml IGF-I of 21-day-groups and 1 ng/ml IGF-I of 28-day-groups. Dose response effects of IGF-I of ALP activity in MC3T3-E1 cells were seen the highest ALP activity at 1ng/ml until 21days and the highest ALP activity at 10 ng/ml of 28 daygroups. The peak times were seen at 7-day group, 14-day group on control group and experimental group respectively, and 1 ng/ml group was the highest ALP activity, From the above results, IGF-I was not seen notable effect on bone nodule formation and decreased ALP activity of MC3T3-E1 cells but the use of IGF-I to mediate biological stimulation of MC3T3-E1 cells shows promise for future therapeutic application.

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A Convenient Method on the Methyl-Ethyl-Ketone Extraction of $^{99m}TcO^-{_4}$ ($^{99m}TcO^-{_4}$의 메틸-에틸-케톤-간편 추출법)

  • Lee, Jong-Du;Lee, Byung-Hyn
    • Journal of Radiation Protection and Research
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    • v.9 no.2
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    • pp.103-111
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    • 1984
  • A convenient method of $^{99m}Tc$-methyl-ethyl-ketone (MEK) extraction technique was developed and a mobile $^{99m}Tc$-extraction generator was designed. The MEK extraction and the phase separation of $^{99m}TcO^-{_4}$ were carried out with a simple procedure in the same container. The shielding of $^{99}Mo$ radioactivity was made with one lead container. The system was simplified by shielding $^{99m}TcO_4{^-}({\gamma}_e=0.14\;MeV)$ separately. $^{99m}TcO^-{_4}\;in\;^{99m}Tc-MEK$ extract was recovered by adsorption and elution only, and therefore, the possibility of volatilization was reduced. The volume of $^{99m}TcO^{-}{_4}$-saline product was reduced to 1 ml by using a small alumina column and the column operation time was shortened. The separation time of $^{99m}Tc$ was reduced to 30 minutes, and the operation was carried out at the outside of the shielding. The system was designed to operate under the condition of bacteria-free.

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The Structure of Econazole Nitrate (에코나졸 나이트레이트의 구조)

  • Seo, Il-Hwan;Jo, Seong-Il;Park, Gwon-Il
    • Korean Journal of Crystallography
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    • v.1 no.1
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    • pp.14-18
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    • 1990
  • Econazole nitrate, 1-{2-[(4-chlorophenyl)methoxy]-2-(2,4-dichlorophenyl) ethy1}-1H-imidazole mono-nitrate.C18 H16 CI13 N3 O4 Mw=444.7 Monoclinic P/2₁c,a=17.337(4)A, b=15.191(5), c=7.601(3)A, β=91.72(2)', V=2000.9A3, Z=4, Dc=1.49g/cm3, Dm=1.45g/cm3(mo-ka)= 0.7107A, μ=4.31cm-1, F(000)=912.0, T=298'K, final R=0.061 for 1330 unique observed reflection. Each of the three ring system for the stars with B,A and C ring in order whilst A and C ring of econazole lie close to the same plane which is nearly 60˚with B ring. The hydrogen binding nitrogen of C ring and oxygen of nitrate contributes to stailization of econazole nitrate. Intr and intermolecular distances and angles are within the values recorded for simiar compounds.

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