• 약학회지
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• 제24권2호
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• pp.117-122
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• 1980

A new application of high pressure liquid chromatography for the determination of fenitrothion and cis- and trans-neopynamin in insecticidal preparations was investigated. Optimum conditions for a good separation and determination were determined; solvent system: dichloromethane + n-hexane = 17 + 83; Bow rate: 0.5ml/min; column: u-porasil ($4mm{\times}3Ocm$); absorbance wavelength: 254nm; 0.05 AUFS and sample size: 30 ul. Recovery of fenicrothion, cis- and trans-neopynamin from mixed artificial preparations was 99.6%, 99.7% and 99.8% respectively. Also reproducibility tests showed that the coefficient of variation was 0.89% for fenitrothion, 0.74% cis-neopynamin and 1.1% for trans-neopynamin. There was no interference with insecticidal preparation containing DDVP, allethrin, S-421 and kerosene. HPLC method was rapid, accurate and it gave better reproducibility and higher sensitivity than any other analytical method. It was considered that HPLC could be greatly applied to the analysis of fenitrothion and neopynamin in insecticidal preparations.

• 식물병연구
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• 제24권4호
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• pp.348-352
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• 2018

A multiplex reverse transcription-polymerase chain reaction (mRT-PCR) assay was developed for the detection of Clover yellow vein virus (ClYVV), Peanut mottle virus (PeMoV), and Tomato spotted wilt virus (TSWV), which were recently reported to infect soybean and azuki bean in Korea. Species-specific primer sets were designed for the detection of each virus, and their specificity and sensitivity were tested using mixed primer sets. From among the designed primer sets, two combinations were selected and further evaluated to estimate the detection limits of uniplex, duplex, and multiplex RT-PCR. The multiplex RT-PCR assay could be a useful tool for the field survey of plant viruses and the rapid detection of ClYVV, PeMoV, and TSWV in leguminous plants.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.142.1-142.1
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• 2003

Sensitive and safe method for visualization of DNA in agarose gels using visible dye is described. To improve the sensitivity, we studied a counterion-dye staining method using methyl orange as a counterion-dye which contributes to reduce excessive background staining by indoine blue. Dye concentrations, PH of staining solution, mixing molar ratio of two dyes, and staining times were optimized for the counterion-dye staining. By the staining with a mixed solution of 0.005% indoine blue and 0.00165% methyl orange in 10% ethanol 0.2M sodium acetate, 8 ng of the 3 kb DNA in an agarose gel was detected within 1hr. (omitted)

• 분석과학
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• 제35권1호
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• pp.1-7
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• 2022

This study suggests a new one-step fluorescent cyanoacylate-fuming method for developing fingerprints by using a CAB mixed with dimethylaminobenzalde (DMAB) and cyanoacylate (CA) in a specific ratio. CAB is prepared by mixing 2.5 % (w/w) DMAB with CA and fumigated at 180 ℃. Under these conditions, developing fingerprints showed the best results. The fuming method using CAB develops latent fingerprints into fluorescence and has a higher sensitivity than CA, and it showed comparable or better contrast to existing fluorescence enhancement methods. It was also applicable on a variety of non-porous surfaces that can be encountered at ordinary times. This method is more useful than conventional fluorescent dyeing methods in that it minimizes damage to fingerprints or samples, makes it easy to manufacture, saves time, and can use existing current equipment as it is.

• Asian Pacific Journal of Cancer Prevention
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• 제14권6호
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• pp.3647-3652
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• 2013

Purpose: To evaluate the diagnostic value of $^{18}F$-FDG PET/CT for detection of bone metastasis in comparison with the efficacies of $^{18}F$-FDG PET/CT, CT, $^{18}F$-FDG PET and conventional planar bone scintigraphy in a series of cancer patients. Methods: Five hundred and thirty patients who underwent both $^{18}F$-FDG PET/CT and bone scintigraphy within 1 month were retrospectively analyzed. The skeletal system was classified into 10 anatomic segments and interpreted blindly and separately. For each modality, the sensitivity, specificity, accuracy, PPV and NPV were calculated and the results were statistically analyzed. Results: Bone metastases were confirmed in 117 patients with 459 positive segments. On patient-based analysis, the sensitivity, specificity, accuracy, PPV and NPV of $^{18}F$-FDG PET/CT were significantly higher than bone scintigraphy, CT and $^{18}F$-FDG PET (P<0.05). On segment-based analysis, the sensitivity of CT, bone scintigraphy, $^{18}F$-FDG PET and $^{18}F$-FDG PET/CT were 70.4%, 89.5%, 89.1% and 97.8%, respectively (P<0.05, compared with $^{18}F$-FDG PET/CT). The overall specificity and accuracy of the four modalities were 89.1%, 91.8%, 90.3%, 98.2% and 90.3%, 90.9%, 89.8%, 98.0%, respectively (P<0.05, compared with $^{18}F$-FDG PET/CT). The PPV and NPV were 89.8%, 87.6%, 85.6%, 97.2% and 85.6%, 93.2%, 92.8%, 98.6%, respectively. Three hundred and twelve lesions or segments were presented as lytic or sclerotic changes on CT images at the corresponding sites of increased $^{18}F$-FDG uptake. In lytic or mixed lesions, the sensitivity of $^{18}F$-FDG PET/CT and $^{18}F$-FDG PET were better than bone scintigraphy, while in osteoblastic lesions bone scintigraphy had a similar performance with $^{18}F$-FDG PET/CT but better than $^{18}F$-FDG PET alone. Conclusion: Our data allow the conclusion that $^{18}F$-FDG PET/CT is superior to planar bone scintigraphy, CT or $^{18}F$-FDG PET in detecting bone metastasis. $^{18}F$-FDG PET/CT may enhance our diagnosis of tumor bone metastasis and provide more information for cancer treatment.

• 대한소아치과학회지
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• 제33권1호
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• pp.1-12
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• 2006

최근 새로 개발된 DIFOTI(Digital Imaging Fiber-Optic Transillumination) 시스템의 초기 법랑질 우식증 진단 능력을 평가하고 임상 적용의 가능성을 타진해 보고자 본 연구를 시행하였다. 임상 연구는 유치 탈락 시기에 근접한 학령기 아동 23명을 대상으로 DIFOTI 이미지 촬영을 시행하고 자연 탈락된 유치 20개를 수거하여 CLSM 촬영 결과를 바탕으로 민감도(sensitivity)와 특이도(specificity)를 산출하였으며 실험실 연구에서는 수거된 40개의 유치를 대상으로 Carbopol 인공 우식 용액으로 1, 2, 4 그리고 8일간 탈회시킨 후 각각 방사선 사진, DIFOTI 이미지 그리고 CLSM와 비교 분석하여 다음과 같은 결론을 얻었다. 1. 구내에서 촬영한 DIFOTI 이미지의 민감도는 0.61이었고, 특이도는 0.63이었다. 2. 실험실에서 인공 탈회시킨 유치의 협설면 초기 우식증에 대한 DIFOTI 이미지의 민감도는 0.71였고, 특이도는 0.75였다.

• Tuberculosis and Respiratory Diseases
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• 제68권4호
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• pp.205-211
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• 2010

Background: Procalcitonin is a well known marker in infection that plays a role in distinguishing between bacterial and viral infections in screening. The aim of the present study was to evaluate the role of procalcitonin in differentiating between 2009 H1N1 influenza pneumonia and community acquired pneumonia of bacterial origin, or mixed bacterial origin and 2009 H1N1 influenza infection. Methods: A retrospective observational study was performed over the 6-month winter period during the 2009 H1N1 influenza pandemic. Ninety-six patient-subjects were enrolled, all of whom had been diagnosed with community acquired pneumonia in emergency department during the study period. On admission, laboratory studies were performed, which included 2009 H1N1 influenza real-time polymerase chain reaction of nasal secretions and procalcitonin on serum; the laboratory values were compared between the study groups. Receiver operating characteristic curve analyses were performed on the resulting data. Results: Compared to those with bacterial or mixed infections (n=62) and bacterial pneumonia with confirmed organisms (n=30), patients with 2009 H1N1 pneumonia (n=34) were significantly more likely to have low procalcitonin levels (p=0.008, 0.001). Using cutoff of value >0.3 ng/mL, the sensitivity and specificity of procalcitonin for detection of patients with confirmed bacterial pneumonia were 76.2% and 60.6%, respectively. A significant difference in procalcitonin was found between 2009 H1N1 pneumonia and pneumonia caused by mixed influenza viral and bacterial infections (0.15 [0.05~0.84] vs. 10.3 [0.05~22.87] ng/mL, p=0.045). Conclusion: Serum procalcitonin measurement may assist in the discrimination between pneumonia of bacterial and of 2009 H1N1 influenza origin. High values of procalcitonin suggest that bacterial infection or mixed infection of bacteria and 2009 H1N1 influenza is more likely.

• Applied Biological Chemistry
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• 제26권2호
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• pp.104-109
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• 1983

phorate와 그 대사산물(代謝産物)들의 잔류분(殘留分) 분석방법(分析方法)을 확립(確立)코자 10% DC-200+8% Reoplex-400+2% QF-1(2 : 1 : 1, w/w/w) on Gas Chrom Q( $80{\sim}100$ mesh), $1.8m{\times}2mm$ ID, borosilicate glass column인 mixed phase column을 이용(利用), flame photometric detector가 부착(附着)된 gas chromatograph로 조사(調査)하였다. 본(本) mixed phase column은 비록 phorate와 5가지 대사산물(代謝産物)을 동시(同時)에 분리(分離)하지는 못하였으나 column온도(溫度)를 programming$(130{\sim}200^{\circ}C,\;5^{\circ}C/min)$함으로써 phoratoxon sulfoxide를 제외(除外)한 5가지 화합물(化合物)의 분리(分離)가 가능(可能)하였다(phoratoxon sulfoxide는 phoratoxon과 중첩되었음). 또는 검출감도(檢出感度)도 매우 높아 $0.05{\sim}3.15ng$범위(範圍)이었다. 한편 토양(土壤)과 채소(菜蔬)에 처리(處理)한 phorate와 그 대사산물(代謝産物)들을 methanol-acetone-benzene(1 : 1 : 1, v/v/v)으로 Soxhlet장치(裝置)에서 12시간(12時間) 추출(抽出)한 후 benzene으로 이들 화합물(化合物)을 분리(分離)한 결과(結果) 90%이상(以上)의 높은 회수율(回收率)(phoratoxon은 약(約) 84%)을 얻었다. 따라서 본(本) mixed phase column은 분리(分離)와 감도면(感度面)에서 그 성능(性能)이 우수(優秀)하여 phorate의 분해(分解)와 대사(代謝)의 연구(硏究)에 효율적(效率的)으로 이용될 수 있을 것이다.

• 한국전기전자재료학회논문지
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• 제23권12호
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• pp.956-960
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• 2010

CuO doped $WO_3-SnO_2$ thick film gas sensors were fabricated by screen printing method on alumina substrates and heat-treated at $350^{\circ}C$ in air. The effects of mixing ratio of $WO_3$ with $SnO_2$ on the structural and morphological properties of $WO_3-SnO_2$ were investigated X-ray diffraction and Scanning Electron Microscope. The structural properties of the $WO_3-SnO_2$:CuO thick film by XRD showed that the monoclinic of $WO_3$ and the tetragonal of $SnO_2$ phase were mixed. Nano CuO was coated on the $WO_3-SnO_2$ surface and then the surface of $WO_3$ was coated with $SnO_2$ particles with $1\sim1.5{\mu}m$ in diameters, as confirmed form the SEM image. The sensitivity of the $WO_3-SnO_2$:CuO sensor to 2000 ppm $CO_2$ gas and 50 ppm $H_2S$ gas for the various ratio of $WO_3$ and $SnO_2$ was investigated. The 4 wt% CuO doped $WO_3-SnO_2$(75:25) tkick films showed the highest sensitivity to $CO_2$ gas and $H_2S$ gas.

• 한국산학기술학회논문지
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• 제4권3호
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• pp.139-144
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• 2003

평면형 마이크로가스센서를 MEMS 기술을 이용하여 제작하였다. NO₂ 가스의 감지를 위한 감지물질로서 이용되는 WO₃ 박막은 텅스텐 타겟을 스퍼터링한 후에 1시간 동안 여러 온도에서 열산화법에 의해 형성하였다. NO₂ 감도(Rgas/Rair)는 열처리 온도에 따른 WO₃ 박막에 대해 조사하였다. 동작온도가 200℃일 때 600℃에서 열처리한 시편의 NO₂가스감도가 가장 높았다. XRD의 결과는 열처리한 시편은 triclinic구조와 orthorhombic구조가 혼합된 다결정상을 보여주었다 또한 시편은 triclinic구조가 적을수록 더 높은 가스 감도를 보여주었다 600℃에서 열처리한 시편의 20℃의 동작온도일 때 5 ppm NO₂ 가스감도는 약 90이었다.