• Title/Summary/Keyword: Mixed infection

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Piroplasma Infections of Canadian Dairy Cattle Imported to GeongBuk, Korea: Piroplasma Infections in Several Farms (경북지방(慶北地方)에 수입(輸入)된 Canada 산(産) 유우(乳牛)의 Piroplasma 감염피해(感染被害)에 관(關)한 조사보고(調査報告)(II) 수개목장(數個牧場) 사육우(飼育牛)의 Piroplasma 감염상황(感染狀況)과 치료성적(治療成績)에 관하여)

  • Son, Jae Young;Yu, Tong Yeul;Kim, Kyo Jun
    • Korean Journal of Veterinary Research
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    • v.12 no.1
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    • pp.59-66
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    • 1972
  • A survey of piroplasma infections, and trials of therapy of dairy cattle imported from Canada were conducted. The studies were carried out by hematological and clinical examinations, from May to September, 1970. The result obtained were summarized as follows: 1. A considerable damages due to Babesia and Theileria infections of dairy cattle were observed in several dairy farms where Pamaquine and Quinuroneum sulphate treatment was applied. 2. The infection was more commonly found in farms where Korean native cattle was raised previously. 3. The dairy cattle raised on pasture which is lightly infected with ticks were mostly infected with Theileria. The infected cattle were easily recovered after treatment with Pamaquine. 4. Leukocytosis and the left shift of neutrophils were observed in the cattle showing mixed infection with Babesia and Theileria.

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Analysis of Grapevine rupestris stem pitting-associated virus in Slovakia Reveals Differences in Intra-Host Population Diversity and Naturally Occurring Recombination Events

  • Glasa, Miroslav;Predajna, Lukas;Soltys, Katarina;Sihelska, Nina;Nagyova, Alzbeta;Wetzel, Thierry;Sabanadzovic, Sead
    • The Plant Pathology Journal
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    • v.33 no.1
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    • pp.34-42
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    • 2017
  • Grapevine rupestris stem pitting-associated virus (GRSPaV) is a worldwide-distributed pathogen in grapevines with a high genetic variability. Our study revealed differences in the complexity of GRSPaV population in a single host. A single-variant GRSPaV infection was detected from the SK30 grapevine plant. On the contrary, SK704 grapevine was infected by three different GRSPaV variants. Variant-specific RT-PCR detection protocols have been developed in this work to study distribution of the three different variants in the same plant during the season. This study showed their randomized distribution in the infected SK704 grapevine plant. Comparative analysis of full-length genome sequences of four Slovak GRSPaV isolates determined in this work and 14 database sequences showed that population of the virus cluster into four major phylogenetic lineages. Moreover, our analyses suggest that genetic recombination along with point mutations could play a significant role in shaping evolutionary history of GRSPaV and contributed to its extant genetic diversification.

A Study on Parasitic Eggs in Soil in Rural Area (一部 農村地域의 寄生蟲卵 土壤 汚染度에 관한 調査硏究)

  • Kim, Woong Ki;Chung, Moon Ho
    • Journal of Environmental Health Sciences
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    • v.10 no.1
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    • pp.67-77
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    • 1984
  • Going to the studies on parasite control carried out in the past. Infection rate among the rural population is contineously decreasing, however, it is still high in Korea. The most important reason for this high infectious rate is the use of manure as fertilizer. This study was aimed at finding the relationship between the infection rate and manure through analizing 83 samples of soil collected from Sa Am-Ri, Kangwon-do in June and July, 1983. The major findings of the study are as follows: 1. 60 cases out of the total sample were carry in at least one or more parasitic eggs, thus revealing 72.3% of positive rate. 2. A total number of 131 parasitic eggs were detected from total sample with an average of 1.6 eggs per 10 g of soil. 3. Asearid eggs more frequently appeared than other parasitic eggs occupying 68.7% or 1.2 eggs in average per 10g of soil. 4. There was revealed positive relationship between infectious rate and frequency of use of manure. (P > 0.05). 5. Infectious rate and average number of parasitic eggs detected were less in the fertilizer mixed with manure and ash than in the soil where only manure was used. (P > 0.05).

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Prevalence and Clinical Manifestations of Malaria in Aligarh, India

  • Asma, Umm-E;Taufiq, Farha;Khan, Wajihullah
    • Parasites, Hosts and Diseases
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    • v.52 no.6
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    • pp.621-629
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    • 2014
  • Malaria is one of the most widespread infectious diseases of tropical countries with an estimated 207 million cases globally. In India, there are endemic pockets of this disease, including Aligarh. Hundreds of Plasmodium falciparum and P. vivax cases with severe pathological conditions are recorded every year in this district. The aim of this study is to find out changes in liver enzymes and kidney markers. Specific diagnosis for P. falciparum and P. vivax was made by microscopic examination of Giemsa stained slides. Clinical symptoms were observed in both of these infections. Liver enzymes, such as AST, ALT, and ALP, and kidney function markers, such as creatinine and urea, were estimated by standard biochemical techniques. In Aligarh district, P. vivax, P. falciparum, and mixed infections were 64%, 34%, and 2%, respectively. In case of P. falciparum infection, the incidences of anemia, splenomegaly, renal failure, jaundice, and neurological sequelae were higher compared to those in P. vivax infection. Recrudescence and relapse rates were 18% and 20% in P. falciparum and P. vivax infections, respectively. Liver dysfunctions and renal failures were more common in P. falciparum patients, particularly in elderly patients. Artesunate derivatives must, therefore, be introduced for the treatment of P. falciparum as they resist to chloroquine as well as sulfadoxine-pyrimethamine combinations.

Multiplex RT-PCR Assay for the Detection of Apple stem grooving virus and Apple chlorotic leaf spot virus in Infected Korean Apple Cultivars

  • Park, Hong-Lyeol;Yoon, Jae-Seung;Kim, Hyun-Ran;Baek, Kwang-Hee
    • The Plant Pathology Journal
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    • v.22 no.2
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    • pp.168-173
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    • 2006
  • To develop the diagnostic method for the viral infection in apple, the partial genes corresponding to the N-terminal region of RNA polymerase of Apple stem grooving virus (ASGV) and coat protein of Apple chlorotic leaf spot virus (ACLSV) were characterized from the infected apple cultivars in Korea. Based on the nucleotide sequences of the characterized partial genes, the virus gene-specific primers were designed for the detection of ASGV and ACLSV infected in species of Malus. The RT-PCR using the primers for the genes of ASGV and ACLSV successfully gave rise to 404 and 566 bp DNA fragments, respectively. Using those viral gene-specific primers, the multiplex RT-PCR assays were also established to diagnose the mixed infection by ASGV and ACLSV simultaneously. Furthermore, the control primers, which have to be included for the RT-PCR as an internal control, were designed using the nucleotide sequence of the gene encoding elongation factor $1{\alpha}(EF1{\alpha})$. This multiplex RT-PCR including the control primers provides more reliable, rapid and sensitive assay for the detection of ASGV and ACLSV infected in Korean apple cultivars.

Studies on the etiological agents in pups with the concurrent intestinal and respiratory tract disorders

  • Park, Hee-myung;Oh, Tae-ho;Youn, Sin-keun;Han, Hong-ryul
    • Korean Journal of Veterinary Research
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    • v.39 no.4
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    • pp.825-831
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    • 1999
  • This study was performed to determine the etiological agents in concurrent disorders in gastrointestinal and respiratory tract. Most of dogs had clinical signs including nasal and ocular discharge, coughing, vomiting, and diarrhea. Of the 22 dogs, seropositive rates of each virus were 54.5% (12/22) against canine distemper virus, 90.9% (20/22) against canine adenovirus 1, 36.4% (8/22) against canine adenovirus 2, 18.2% (4/22) against canine parvovirus, 81.8% (18/22) against canine hepatitis virus and 59.1% (13/22) against canine coronavirus. Canine distemper virus and canine parvovirus infection were 54.6% (12/22) in histopathological examination. In addition, mixed infections of canine distemper virus and adenovirus 2 were 9.1% (2/22). While simple infection of canine adenovirus 2 were 9.1% (2/22). E coli and Staphylococcus spp were isolated in facts as a rate of 72.7% (16/22) and 40.9% (9/22), respectively. Conclusionally, it is also estimated that environmental stress might be one of the causative factors.

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One Case of Ludwig's Angina with Mediastinal Fistula and Pneumonia (종격동루공 및 폐렴을 동반한 Ludwig's angina의 1례)

  • 한경수;홍정애;정덕희;김춘길
    • Proceedings of the KOR-BRONCHOESO Conference
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    • 1979.05a
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    • pp.9.1-10
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    • 1979
  • The authors have recently observed a case of Ludwig's angina with forming mediastinal fistula & pneumonia. The Ludwing's angina is the cellulitis of the mouth floor and neck, ie, of the sublingual space. The suppurative inflammation of this space develops from dental infection, and can also develop from ulceration or inflammation of the mouth floor and the tongue base, lingual tonsillitis or salivary calculi. The main causes are characterized as mixed infection which hemolytic streptococcus and staphylococcus are considered to be pathognomic organisms. It may be followed as complications of mediastinal extension, parapharyngeal extension and laryngeal edema. This is the report on this case with literature review.

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Microfloras of otitis externa and normal external ear canals in dogs (개에서 외이도염 및 정상외이도에서 분리한 세균총에 대한 연구)

  • Kim, Ki-hyang;Choi, Won-phil
    • Korean Journal of Veterinary Research
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    • v.39 no.3
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    • pp.566-574
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    • 1999
  • This study was conducted to examine the outbreak rate and the causative agents of otitis externa in 26 dogs (49 ears ; 23 dogs = bilateral, 3 dogs = unilateral), and the normal microfloras of external ear canal in 68 dogs(133 ears ; 65 dogs = bilateral, 3 dogs = unilateral ) in Taegu, 1997. The breed, living environment, sex, age and season distribution of otitic dogs were as follows : Dogs with erect and hairy ears(42.3%), pendulous and hairy ears(38.5%), indoor(92.3%), female(65.4%) and below one year old(38.5%) were more prevalent. According to season, otitis externa was mainly occurred between July and October. The major causative agents of canine otitis externa were Malassezia pachydermatis (32.7%), Staphylococcus aureus (26.5%) and S intermedius (16.3%). In the microorganism isolated 39 otitic ear canals, single infection was 53.8% and mixed infection was 46.2%. The normal microfloras of canine external ear canal were fungi including M pachydermatis, Aspergillus spp, Microsporum canis, Alternaria spp, Verticillium spp and Yeast, and bacteria including Staphylococcus spp(10 species including S xylosus), Bacillus spp, Corynebacterium spp, Listeria spp, Actinomyces pyogenes and Escherichia coli. No growth was 34.6%.

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Prevalence of Intestinal Parasites Infection of Dogs in Chonbuk Province (전북지방 개의 장내기생충 감염실태)

  • 양홍지;윤여백;이흥재;최인방;박태욱;서창섭
    • Korean Journal of Veterinary Service
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    • v.15 no.1
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    • pp.7-16
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    • 1992
  • In order to detection of the Intestinal parasites, 503 fecal samples were taken from mongorel-and pad-dogs in Chonbuk province. The prevalence and identification of intestinal parasites were determined by the fecal examinations using the floatation and /or sedimentation methods and microscopical examination, respectively. The results were obtained as follows 1. Fifty-nine percent (297 dogs) from 503 fecal samples were detected eggs. In seasonal detection rate of eggs, Summer was 30.3%, Autumn 26.4%, Winter 22.3% and Spring 21.0%, in order, 2. A total of 20 kinds of eggs were isolated from feces, and it was identified 75.7% as Nematoda(320 dogs), 5.6% as Cestoda(24 dogs) and 1.4% as Trematoda(6 dogs), and 17.2% as Protozoa(73 dogs). The isolates were identified as Ancylostoma caninum (30.4%, 153 dogs), Isospora spp. (14.3%, 72 dogs), Toxocara canis(11.1%, 56 dogs), Toxascaris leonina(5.8%, 29 dogs) , Uncineria stenocephala or Physaloptera spp. (5.4%, 27 dogs), Trichuris vulpis(2.4%, 12 dogs) and the others, single or in combination. 3. In mixed infection such as single, double, triple and quadraple was 63.6%, 31.7%, 3.4% and 1.3%, respectively.

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A survey on gastrointestinal parasites of Korean water deer (Hydropotes inermis agyropus) in Chungbuk province (충북지역 야생 한국고라니의 내부기생충 감염률 조사)

  • Choe, Seong-Jun;Na, Ki-Jeong;Jee, Cha-Ho
    • Korean Journal of Veterinary Service
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    • v.34 no.3
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    • pp.259-263
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    • 2011
  • Wildlife has been recognized that has important role as reservoir hosts of disease affecting both people and livestocks. However, information intestinal helminths of Korean water deer were limited. We obtained 20 feces from Korean water deers that were transferred to veterinary hospital in Chungbuk National University from May 2010 to June 2011. Parasite ova were collected using saturated zinc sulfate floatation method. Parasite eggs were examined and measured using light microscope. The identification of parasitic eggs was done by morphological characters. The overall infection rate of parasite was 85% (n=17). The eggs were observed as being Nematodirus spp. (n=14), strongyles egg (n=11), Capillaria spp. (n=9), Trichuris spp. (n=7), Eimeria spp. (n=4), Ogmocotyle spp. (n=3), and Strongyloides (n=1). Mixed infection rate was observed as being single 15% (n=3), double 20% (n=3), triple 15% (n=3), quadruple 30% (n=6), and quintuple 5% (n=1). The Ogmocotyle spp. was first reported in Korea.