• Asian-Australasian Journal of Animal Sciences
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• 제14권1호
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• pp.48-53
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• 2001

An in vitro study was conducted to examine the effect of sources and the addition levels of carbohydrates on fermentation characteristics, bacterial growth, and hydrogenation of linoleic acid ($C_{18:2}$) by mixed ruminal bacteria. Starch and cellobiose were added to the 200 ml non-selective basal media at the levels of 0.20 and 0.35% (w/v), respectively. Linoleic acid (66.8~79.6 mg) in the absorbed form into the pieces of nylon cloth was also added to the media of 5 treatments including control which was not added with carbohydrate. Three mls of rumen fluid strained through 12 layers of cheese cloth were added to each medium, and were incubated anaerobically in the shaking incubator of $39^{\circ}C$ for 24 hours. During 24 h incubation the pH in incubation media of all treatments was maintained above 6.6 by the addition of sodium bicarbonate. The pH and ammonia concentration of incubation media were not clearly influenced by the sources and addition levels of carbohydrates while additions of carbohydrates increased (p<0.0001) VFA concentration at the 24 h incubation. Molar proportions of acetate were reduced (p<0.0004) while those of propionate were increased (p<0.0006) by the addition of carbohydrates. But the differences in concentration and molar proportions of the VFA were small between the sources or the addition levels. Bacterial growth was faster (p<0.0004) in the starch added treatments than in the cellobiose added ones and control, but no differences were found between addition levels. Increased (p<0.0487) hydrogenation was observed from the starch added treatments compared to the cellobiose added ones, but there was no difference between addition levels.

• Asian-Australasian Journal of Animal Sciences
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• 제30권4호
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• pp.495-504
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• 2017

Objective: Manipulating the fermentation to improve the performance of the ruminant has attracted the attention of both farmers and animal scientists. Propionate salt supplementation in the diet could disturb the concentration of propionate and total volatile fatty acids in the rumen. This study was conducted to evaluate the effect of calcium propionate supplementation on the ruminal bacterial community composition in finishing bulls. Methods: Eight finishing bulls were randomly assigned to control group (CONT) and calcium propionate supplementation (PROP) feeding group, with four head per group. The control group was fed normal the total mixed ration (TMR) finishing diet, and PROP group was fed TMR supplemented with 200 g/d calcium propionate. At the end of the 51-day feeding trial, all bulls were slaughtered and rumen fluid was collected from each of the animals. Results: Propionate supplementation had no influence the rumen fermentation parameters (p>0.05). Ruminal bacterial community composition was analyzed by sequencing of hypervariable V3 regions of the 16S rRNA gene. The most abundant phyla were the Firmicutes (60.68%) and Bacteroidetes (23.67%), followed by Tenericutes (4.95%) and TM7 (3.39%). The predominant genera included Succiniclasticum (9.43%), Butyrivibrio (3.74%), Ruminococcus (3.46%) and Prevotella (2.86%). Bacterial community composition in the two groups were highly similar, except the abundance of Tenericutes declined along with the calcium propionate supplementation (p = 0.0078). Conclusion: These data suggest that the ruminal bacterial community composition is nearly unchanged by propionate supplementation in finishing bulls.

• Journal of Korean Neurosurgical Society
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• 제30권3호
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• pp.366-370
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• 2001

Objective : The authors present three cases of brain tumors in which epidural hematomas(EDHs) were developed postoperatively in the remote areas from craniotomy sites. The preventive tactics as well as possible mechanisms of development of remote EDH are discussed. Material and Methods : The magnetic resonance imagings of three patients revealed a left lateral ventricular mass located just aside of foramen Monro in a 27-year-old male, a large cystic mass in the temporal lobe in a 35-year-old male, and a partially calcified pineal mass in a 27-year-old male patient. The surgical removals of these tumors were performed without any noticeable events during surgery via left frontal transcortical transventricular approach for lateral ventricular tumor, left temporal craniotomy for cystic temporal tumor, and right occipital transtentorial approach for pineal tumor. Results : Postoperative EDHs remote from the sites of craniotomy were detected by the immediate postoperative computerized tomographic scans. We obtained good outcomes without any morbidity in all three patients with emergent evacuation of the hematoma. The pathologic diagnoses were lateral ventricular ependymoastrocytoma, temporal craniopharyngioma and mixed germinoma of the pineal region. Conclusion : It is postulated that a sudden reduction of intracranial pressure(ICP) at the time of tumor removal may strip the dura from the inner table of the skull to cause EDH from the remote site of craniotomy. Gradual reduction of ICP with slow drainage of cerebrospinal fluid before tumor removal as well as lowering the head position of patient during surgery might be helpful for preventing this unusual complication.

• 한국산학기술학회논문지
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• 제17권7호
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• pp.237-244
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• 2016

모르타르를 포함한 건설재료의 작업성은 시공 품질을 결정짓는 중요한 요소 중 하나이다. 콘크리트 등 건설재료의 작업성을 정량적으로 표현할 수 있는 레올로지 특성은 상용화된 레오미터를 통해 측정할 수 있지만, 경제적 또는 공간적 제약으로 인해 실제 시공현장에서의 사용에는 한계가 있다. 많은 건설현장에서는 콘크리트 또는 모르타르의 작업성을 평가하기 위해 슬럼프 플로 시험 등을 통해 정성적으로 재료의 시공성능을 평가하고 있다. 이 논문에서는 시공현장에서 간편하게 모르타르의 레올로지 특성을 측정할 수 있는 채널 플로 시험과 레올로지 특성과의 상관관계식을 제안하고자 한다. 이를 위해서 VOF기법을 이용한 유동 해석을 진행하였으며, 유동 시뮬레이션 결과를 로그함수 모델로 일반화하여 채널 플로 길이와 정지시간을 데이터베이스화 하였다. 수립된 데이터베이스를 기준으로 최소 자승법을 이용한 회귀분석을 진행하였고, 결과적으로 6개의 매개변수로 구성된 모르타르의 채널 플로 시험 결과와 레올로지 특성의 상관관계식을 도출하였다. 또한, 상용 레오미터로 측정한 레올로지 특성과 채널 플로 실험결과로부터 예측된 레올로지 특성을 비교검증 함으로써 제안된 상관관계식의 현장적용 가능성을 확인하였다.

• 한국태양에너지학회 논문집
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• 제30권4호
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• pp.92-99
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• 2010

The purpose of this study is to apply pressurized plenum under floor air conditioning system to office areas to understand characteristics of indoor thermal environment based on forms of diffusers. For doing this, the author conducted experiment of module measurement, and based on the results, analyzed indoor temperature distribution and velocity distribution based on direction of diffusion by using Computational Fluid Dynamics(CFD), and estimated the Predicted Mean Vote(PMV) of residents based on forms of diffusers to present the optimal air conditioning of the pressurized plenum under floor air conditioning system in heating. The results of this study are as follows. First, as for forms of diffusers, distributed diffusers rather than conical and grill diffusers were favorable in maintaining $24^{\circ}C$, the established temperature in heating, were active in velocity flowing, and were wide in a radius of diffusion. Second, as for position of pressurizing, the difference between upper and lower temperature was wider in center, lateral, and dispersed pressurizing (in order). As for velocity distribution, the velocity was more increased in lateral, center, and dispersed pressurizing(in order), indicating that dispersed pressurizing maintained uniform thermal environment. Third, as for diffusion direction, mixed direction showed less difference between upper and lower temperature and the difference in velocity between center and lateral part was 0.01m/1, indicating that it maintained uniform thermal environment. Fourth, as for the PMV of residents based on the forms of diffusers, the dispersed type showed(+) values above (0) when applied variably based on the position of diffuser, presenting thermal feeling of "being comfortable" to residents.

• 한국세라믹학회지
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• 제48권1호
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• pp.26-33
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• 2011

The glass in the system of CaO-$SiO_2-P_2O_5$ and the corresponding glass-ceramics are prepared for bone cements and the behaviors of the hardening and hydroxyapatite formation were studied for the glass and glass-ceramic powders. The glass crystallized into apatite, $\alpha$-wollastonite and $\beta$-wollastonite depending on the glass composition when they were heat-treated at $950^{\circ}C$ for 4 h. A DCPD (dicalcium phosphate dihydrate : $CaHPO_4{\cdot}2H_2O$) was developed when the prepared glass and glass-ceramic powders were mixed with 3M-$H_3PO_4$ solution. The DCPD (Ca/P=1.0) transformed to HAp (Ca/P=1.67) when the bone cement was soaked in simulated body fluid (SBF), and this HAp formation strongly depended on the releasing capacity of $Ca^{2+}$ ions from the glass and glass-ceramic cements. The glass-ceramic bone cement containing $\alpha$-wollastonite crystals showed faster transformation of DCPD to HAp than other glass-ceramics containing $\alpha$- and $\beta$-wollastonite crystals. No hydroxyapatite was observed when the glass-ceramic bone cement containing apatite crystals (36P6C) was soaked in SBF even for 1 month, because no $Ca^{2+}$ ion can be released from the stable apatite crystals.

• 유체기계공업학회:학술대회논문집
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• 유체기계공업학회 2006년 제4회 한국유체공학학술대회 논문집
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• pp.451-454
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• 2006

This paper describes the results of cold flow test and hot firing tests of an uni-element coaxial swirl injector and hot firing tests of a subscale combustor, as to the development effort of coaxial swirl injector for high performance liquid rocket engine combustor. A major design parameter for coaxial swirl injector is the recess number of a bi-swirl injector. The results of hot firing tests of the uni-element injector combustor and the sub-scale combustor are analyzed to investigate the effect of the recess number influencing on the combustion performance and pressure fluctuation. The test results of a cold flow test of the unielement combustor shows that it was shown that the change in recess number has significant effect on mixing characteristics and efficiency, while the effect of recess number on atomization characteristic is not The results of a series of firing tests using unielement and subscale combustor show that the recess length significantly affects the hydraulic characteristics, the combustion efficiency, and the dynamics of the liquid oxygen/kerosene bi-swirl injector. As a point of combustion performance, combustion efficiencies are 90% for unielement combustor and 95% for subscale combustor. The difference in the characteristic velocities between the unielement combustor and the subscale combustor may be caused by the difference in thermal loss to the combustor wall and the relative lengths of the combustion chamber. For a mixed type coaxial swirl combustor, the pressure drop across the injector increases as recess number becomes larger. The low frequency pressure fluctuation observed in unielement combustor can be related to the propellant mixing characteristics of the coaxial bi-swirl injector. The effect of the recess number on the pressure fluctuation inside the combustion chamber is more significant in un i-element combustor than the subscale combustor, of which the phenomena are also observed in time domain and frequency domain.

• 대한기계학회논문집B
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• 제35권4호
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• pp.385-390
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• 2011

본 연구에서는 미세채널과 나노채널의 교차부에 불균형 동전기성을 이용한 미세혼합기를 개발하였다. 채널 내 용액의 혼합은 인가된 전압에 의한 전기삼투유동과 미세채널과 나노채널 교차 부에서의 불균형 동전기성에 의한 와류현상에 의해 이루어진다. 미세채널은 PDMS 을 이용하여 소프트리소그래피 공정으로 제작하였고, 나노채널은 미세채널의 특정위치에 전기적 충격에 의한 PDMS 의 파괴로 매우 간단하게 제작하였다. 혼합성능을 평가하기 위하여 형광물질인 Rhodamine B 용액을 이용하여 혼합 전과 후의 형광 분포를 분석하였으며, 약 90%의 혼합을 얻을 수 있었다. 본 연구의 미세혼합기는 복잡한 공정을 요구하지 않고 매우 간단하게 제작되었으며, 생화학시료 분석을 위한 미세시스템에 활용될 수 있다.

• Asian-Australasian Journal of Animal Sciences
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• 제18권6호
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• pp.816-824
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• 2005

The effects of the nonionic surfactant Tween 80 and a mixture of fibrolytic enzymes on total tract digestion, in situ disappearance (ISD) and ruminal fermentation characteristics of orchardgrass hay and barley grain were investigated in a 4${\times}$4 Latin square experiment with 4 non-lactating Holstein cows and 4 diets in 4 periods. Cows were offered a total mixed ration of 50% rolled barley grain and 50% orchardgrass hay treated with either 1) water (control), 2) 0.2% (vol/wt) Tween 80, 3) 0.2% (vol/wt) hydrolytic enzyme, or 4) 0.2% hydrolytic enzyme plus 0.2% Tween 80. Total tract digestibility coefficients of DM, nitrogen, NDF and ADF were not affected (p>0.05) by dietary treatment. Compared to the control, the rate of ISD of DM from orchardgrass hay was faster (p<0.05) in cows receiving diets treated with the enzyme alone or with enzyme plus Tween 80 (0.06/h vs. 0.076 and 0.069/h). The rate of digestion was lower (p<0.05) as compared to control when barley grain was treated with these additives. Ruminal fluid pH and concentrations of total VFA, acetate, isobutyrate and butyrate were not affected (p>0.05) by treatments. Cows that consumed diets treated with enzyme plus Tween 80 had higher (p<0.05) ruminal concentrations of propionate and isovalerate, and lower (p<0.05) acetate:propionate ratios. Compared to the control, microbial protein synthesis tended (p = 0.13) to increase with the addition of enzyme to the diet while nonammonia nitrogen flow to the duodenum increased (p<0.05) with both enzyme and Tween 80 treatments. The study indicated that fibrolytic enzymes alone or in combination with Tween 80 could enhance ISD of orchardgrass hay and ruminal concentrations of propionate, valerate and iso-valerate, but do not affect total tract digestibility.

• Asian-Australasian Journal of Animal Sciences
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• 제28권3호
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• pp.343-350
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• 2015

Cocoa pod is among the by-products of cocoa (Theobroma cacao) plantations. The aim of this study was to apply a number of treatments in order to improve nutritional quality of cocoa pod for feeding of ruminants. Cocoa pod was subjected to different treatments, i.e. C (cocoa pod without any treatment or control), CAm (cocoa pod+1.5% urea), CMo (cocoa pod+3% molasses), CRu (cocoa pod+3% rumen content) and CPh (cocoa pod+3% molasses+Phanerochaete chrysosporium inoculum). Analysis of proximate and Van Soest's fiber fraction were performed on the respective treatments. The pods were then subjected to an in vitro digestibility evaluation by incubation in rumen fluid-buffer medium, employing a randomized complete block design (n = 3 replicates). Further, an in vivo evaluation of the pods (35% inclusion level in total mixed ration) was conducted by feeding to young Holstein steers (average body weight of $145{\pm}3.6kg$) with a $5{\times}5$ latin square design arrangement (n = 5 replicates). Each experimental period lasted for 30 d; the first 20 d was for feed adaptation, the next 3 d was for sampling of rumen liquid, and the last 7 d was for measurements of digestibility and N balance. Results revealed that lignin content was reduced significantly when cocoa pod was treated with urea, molasses, rumen content or P. chrysosporium (p<0.01) with the following order of effectiveness: CPh>CAm>CRu>CMo. Among all treatments, CAm and CPh treatments significantly improved the in vitro dry matter and organic matter digestibility (p<0.05) of cocoa pod. Average daily gain of steers receiving CAm or CPh treatment was significantly higher than that of control (p<0.01) with an increase of 105% and 92%, respectively. Such higher daily gain was concomitant with higher N retention and proportion of N retention to N intake in CAm and CPh treatments than those of control (p<0.05). It can be concluded from this study that treatment with either urea or P. chrysosporium is effective in improving the nutritive value of cocoa pod.