• Journal of Microbiology and Biotechnology
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• 제18권6호
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• pp.1164-1169
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• 2008

We developed multiplex RT-PCR assays that can detect and identify 12 hemagglutinin (H1-H12) and 9 neuraminidase (N1-N9) subtypes that are commonly isolated from avian, swine, and human influenza A viruses. RT-PCR products with unique sizes characteristic of each subtype were amplified by multiplex RT-PCRs, and sequence analysis of each amplicon was demonstrated to be specific for each subtype with 24 reference viruses. The specificity was demonstrated further with DNA or cDNA templates from 7 viruses, 5 bacteria, and 50 influenza A virus-negative specimens. Furthermore, the assays could detect and subtype up to $10^5$ dilution of each of the reference viruses that had an original infectivity titer of $10^6\;EID_{50}/ml$. Of 188 virus isolates, the multiplex RT-PCR results agreed completely with individual RT-PCR subtyping results and with results obtained from virus isolations. Furthermore, the multiplex RT-PCR methods efficiently detected mixed infections with at least two different subtypes of influenza viruses in one host. Therefore, these methods could facilitate rapid and accurate subtyping of influenza A viruses directly from field specimens.

• 대한환경공학회지
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• 제28권4호
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• pp.362-367
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• 2006

도시 하수처리장에서 수집되는 폐활성 슬러지는 유기성 물질을 다량 함유하고 있는 바이오매스이다. 하지만, 대부분의 연구 결과 폐활성 슬러지를 이용한 생물학적 수소생산율은 매우 낮다고 보고되고 있다. 본 연구에서는 폐활성 슬러지를 산, 알카리 처리, 기계적 처리, 열처리, 오존 처리, 초음파 처리 등의 전처리에 대한 효과를 살펴보았다. 전처리 실험결과, 폐활성 슬러지 내의 유기물질들은 가용화되었으며 $SCOD_{Cr}$값으로 약 14.6배의 증가를 보였다. 열처리된 혐기성 슬러지를 이용하여 폐활성 슬러지로부터 최적의 생물학적 수소생산을 위한 실험은 전처리 방법에 대한 효과 및 완충용액의 효과, 수소분압, 그리고 염소이온의 농도 등에 대하여 회분식 조건에서 살펴보았다. 실험결과 효과적인 전처리 방법 및 완충용액의 첨가, gas sparging 등의 방법에 의한 낮은 수소분압인 경우에 수소생산율이 0.52 mmol $H_2/g$-DS(Dried Solids)로 크게 증가함을 확인하였다.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2005년도 ICCAS
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• pp.1431-1434
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• 2005

In the steel production line, the molten metal of a furnace is transformed into billet and then moves to the heating furnace of the hot rolling mill. This paper describes about the development of recognition system for the characters, which was marked at the billet material by use template-marking plate and hand written method, in the steel plant. For the recognition of template-marked characters, we propose PSVM algorithm. And for the recognition of hand written character, we propose combination methods of CCD algorithm and PSVM algorithm. The PSVM algorithm need some more time than the conventional KLT or SVM algorithm. The CCD algorithm makes shorter classification time than the PSVM algorithm and good for the classification of closed curve characters from Arabic numerals. For the confirmation of algorithm, we have compared our algorithm with conventional methods such as KLT classifier and one-to-one SVM. The recognition rate of experimented billet characters shows that the proposing PSVM algorithm is 97 % for the template-marked characters and combinational algorithm of CCD & PSVM is 95.5 % for the hand written characters. The experimental results show that our proposing method has higher recognition rate than that of the conventional methods for the template-marked characters and hand written characters. By using our algorithm, we have installed real time character recognition system at the billet processing line of the steel-iron plant.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제11권4호
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• pp.1972-1986
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• 2017

With the advent of the knowledge economy, science and technology resources have played an important role in economic competition, and their optimal allocation has been regarded as very important across the world. Thus, allocation optimization research for regional science and technology resources is significant for accelerating the reform of regional science and technology systems. Regional science and technology resource allocation optimization is modeled as a double-layer optimization model: the entire system is characterized by top-layer optimization, whereas the subsystems are characterized by bottom-layer optimization. To efficaciously solve this optimization problem, we propose a mixed search method based on the orthogonal genetic algorithm and sensitivity analysis. This novel method adopts the integrated modeling concept with a combination of the knowledge model and heuristic search model, on the basis of the heuristic search model, and simultaneously highlights the effect of the knowledge model. To compare the performance of different methods, five methods and two channels were used to address an application example. Both the optimized results and simulation time of the proposed method outperformed those of the other methods. The application of the proposed method to solve the problem of entire system optimization is feasible, correct, and effective.

• Journal of Ecology and Environment
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• 제32권1호
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• pp.61-65
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• 2009

We compared two nondestructive methods for leaf area estimation using leaves of 16 common plant species classified into six types depending on leaf shape. Relatively good linear relationships between actual leaf area (LA) and leaf length (L), width (W), or the product of length and width (LW) were found for ordinary leaves with lanceolate, oblanceolate, linear and sagitttate shapes with entire margins, serrate margins, mixed margins with a entire form and shallow lobes, and ordinary incised margins. LA was better correlated with LW than L or W, with $R^2$ > 0.91. However, for deeply incised lobes, LA estimation using LW showed low correlation coefficient values, indicating low accuracy. On the other hand, a method using photographic paper showed a good correlation between estimates of area based on the mass of a cut-out leaf image on a photographic sheet (PW) and actual leaf area for all types of leaf shape. Thus, the PW method for LA estimation can be applied to all shapes of leaf with high accuracy. The PW method takes a little more time and has a higher cost than leaf estimation methods using LW based on leaf dimensions. These results indicate that researchers should choose their nondestructive LA estimation method according to their research goals.

• 산업경영시스템학회지
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• 제42권1호
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• pp.8-20
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• 2019

The vehicle routing problem is one of the vibrant research problems for half a century. Many studies have extensively studied the vehicle routing problem in order to deal with practical decision-making issues in logistics. However, developments of new logistics strategies have inevitably required investigations on solution methods for solving the problem because of computational complexity and inherent constraints in the problem. For this reason, this paper suggests a simulated annealing (SA) algorithm for a variant of vehicle routing problem introduced by a previous study. The vehicle routing problem is a multi-depot and multi-trip vehicle routing problem with multiple heterogeneous vehicles restricted by the maximum permitted weight and the number of compartments. The SA algorithm generates an initial solution through a greedy-type algorithm and improves it using an enhanced SA procedure with three local search methods. A series of computational experiments are performed to evaluate the performance of the heuristic and several managerial findings are further discussed through scenario analyses. Experiment results show that the proposed SA algorithm can obtain good solutions within a reasonable computation time and scenario analyses show that a transportation system visiting non-dedicated factories shows better performance in truck management in terms of the numbers of vehicles used and trips for serving customer orders than another system visiting only dedicated factories.

• 대한수의학회지
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• 제61권4호
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• pp.40.1-40.6
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• 2021

Acid-base disorder is a common problem in veterinary emergency and critical care. Traditional methods, as well as the Stewart method based on strong ion difference concepts and the Fencl-Stewart method, can be used to analyze the underlying causes. On the other hand, there are insufficient comparative study data on these methods in cats. From 2018 to 2020, 327 acid-base analysis data were collected from 69 sick and 18 healthy cats. The three most well-known methods (traditional method, Stewart method, and Fencl-Stewart method) were used to analyze the acid-base status. The frequency of acid-base imbalances and the degree of variation according to the disease were also evaluated. In the traditional acid-base analysis, 5/69 (7.2%) cats showed a normal acid-base status, and 23.2% and 40.6% of the simple and mixed disorders, respectively. The Fencl-Stewart method showed changes in both the acidotic and alkalotic processes in 64/69 (92.8%), whereas all cats showed an abnormal status in the Fencl-Stewart method (semiquantitative approach). The frequencies of the different acid-base imbalances were identified according to the analysis method. These findings can assist in analyzing the underlying causes of acid-base imbalance and developing the appropriate treatment.

• 보건교육건강증진학회지
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• 제26권1호
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• pp.141-158
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• 2009

Community-Based Participatory Research(CBPR) has gained attention as a public health approach to develop community health interventions to address health disparities in recognition of the community relevance of specific health issues associated with social determinants of health. It emphasizes community involvement in equal partnership with researchers and public health professionals to address community-identified needs. The characteristics and principles of CBPR discussed in this paper highlight participatory nature, capacity development, partnership building, and process-orientation of CBPR. A 6-step process model for community empowerment is then introduced as a CBPR operationalization strategy. Mixed methods research approaches are valuable in CBPR as well as process evaluation. For the application of CBPR in Korean contexts, the Diffusion of Innovation theory is suggested as a theoretical framework for implementation. Building public health partnerships between public and private sectors to create partnership synergy is a necessary condition for successful CBPR for health promotion in Korea. Accompanying critical factors for the CBPR application include: common understanding of CBPR and its values, establishment of the definition of 'community,' 'community-based' and 'participation' in community health, development of accommodating research infrastructure for CBPR, recognition of the importance of program evaluation (particularly process evaluation), and training CBPR specialists.

• 유통과학연구
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• 제21권2호
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• pp.45-52
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• 2023

Purpose: The Malaysia Education Blueprint is primarily concerned with the transformation of students' minds through the curriculum offered at the school level (2013-2025). Diversity in the application of teaching and learning methods is one means of achieving the transformation of students' minds through the Secondary School Standard Curriculum. Consequently, the production of ExSheetLink's Module for Accounting Education is the primary outcome of this study, which had three objectives: the need for ExSheetLink's Module in the process of producing financial statements for Accounting Students in secondary school to the Accounting Teacher; and the design of ExSheetLink's Module that meets the entire process in the production of financial statements for Accounting Students in secondary school based on the Documents Curriculum and the Accounting Students' needs. Research design, data and methodology: This study outlines the research framework for module development in accordance with the Design and Development Research Method, which combines multiple research techniques (Mixed Method). Results: The development of ExSheetLink's Module is completed and can be used for the level of effectiveness purposes. Conclusion: The transformation of Accounting Students' minds is a success thanks to the ExSheetLink Module. Researchers also suggested that all Malaysian Secondary School accounting students test the ExSheetLink Module.

• 아태비즈니스연구
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• 제14권1호
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• pp.381-396
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• 2023

Purpose - The purpose of this study was to analyze environmental and labor issues and related norms, which are new trade issues that are expanding in bilateral and multilateral trade agreements, and examine the possible impact of these norms on domestic fisheries policies. Design/methodology/approach - In this study, literature research was used as the main research methods. The comparative analysis of international norms and multilateral trade agreements texts related to the environment, labor and trade were conducted. Findings - The new trade norms in the fisheries sector can be represented by labor and environment issues. Since domestic environmental and labor standards do not fully meet the standards of the multilateral trade agreements, it is necessary to ensure that domestic norms are supplemented and relevant policies are newly established through a review of international law on environment and labor. Research implications or Originality - This study confirmed that international norms related to labor and environment in the fisheries sector are mixed with soft norms and binding norms, and each norm is linked in a multi-layered and mutual way. Such international norms are being strengthened in connection with trade agreements and issues.