• Title/Summary/Keyword: Mix-Method

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Application of mix LPS-ELISA for monitoring of antibodies to major serogroups of Salmonella in animal (Mix LPS-ELISA법을 이용한 살모넬라균 복합감염항체 동시 모니터링)

  • Lee, Hee-soo;Im, Suk-kyoung;Woo, Seng-ryong;Yi, You-young;Kim, Jong-yeom;Joo, Yi-seok;Kim, Jong-man
    • Korean Journal of Veterinary Research
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    • v.38 no.4
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    • pp.793-802
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    • 1998
  • Salmonellosis caused by a number of serotypes of Salmonella is an infectious, acute or chronic, zoonotic disease and characterized by enteritis and diarrhea, septicemia in animal. In these studies we investigated the prevalent serotypes of Salmonella causing animal salmonellosis in Korea and the 71 strains of Salmonella spp. were isolated from materials such as mesenteric lymph nodes, fecal samples from slaughtered animal. With the identification test results, the most prevalent serotypes were, in order, S stanley 31 strains (43.7%), S typhimurium 19 strains (26.8%) and S montevideo 11 strains (15.5%), respectively. And we could establish the method for detection of antibodies to broad variety of Salmonella serotypes. Lipopolysaccharide(LPS) antigen extracted from Salmonella was more sensitive and specific than outer membrane protein antigen from that for detection of Salmonella antibody by using an indirect ELISA. The optimal concentration of antigen was 100ng/ml of LPS, the dilutions of conjugate and serum were 1 : 1,000~2,000 and 1 : 200~400, respectively. The mix LPS-ELISA which was used by mixing LPS from S typhimurium (group B), S choleraesuis (group C) and S enteritidis (group D) were more rapid and effective than that used LPS from individual strain for detection of Salmonella serogroup O4, O7 and O9 antibody at the same time. We could obtain the high values of optical density ($0.73{\pm}0.32$) by mix LPS-ELISA on the farm which had occurred salmonellosis, but very low values of $0.17{\pm}0.06$ on the negative farm of salmonellosis. So, the mix LPS-ELISA may be used to monitor the serological surveillance for the presence of infection with a number of serotypes of Salmonella and would be useful for prevention and control of salmonellosis in animal.

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Sorption and Leaching Characteristics of Diesel-Contaminated Soils Treated by Cold Mix Asphalt (Cold Mix Asphalt로 처리한 디젤 오염 토양의 흡착 및 용출특성)

  • Seo Jin-Kwon;Hwang Inseong;Park Joo-Yang
    • Journal of Soil and Groundwater Environment
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    • v.9 no.4
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    • pp.24-31
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    • 2004
  • A cold mix asphalt (CMA) treatment process was proposed as a tool to recycle soils contaminated with petroleum hydrocarbons. Experimental studies were conducted to characterize performances of the CMA process in treating soils contaminated with diesel or diesel compounds. From the screening experiments, it was found that performances of five types of asphalt emulsions that contained a cationic or an anionic or a nonionic surfactant were not substantially different. In consideration of higher affinity for soils and higher sorption coefficients obtained, an emulsion containing Lauryl Dimethyl Benzyl Ammonium Chloride (LDBAC) was selected as a promising asphalt emulsion for treating diesel-contaminated soils. When the asphalt emulsion LDBAC was applied to treat three compounds that originated from diesel, the removal efficiencies obtained in the order of decreasing efficiencies were as follows: docosane > pentadecane > undecane. Leaching experiments on the specimen formulated by the emulsion LDBAC found that the selected treatment method could treat soils with diesel concentrations as high as 10,000 mg/kg. Leaching of the diesel from the specimen was controlled by diffusion for the first four days and then leaching rate diminished substantially. The latter behavior was characterized as depletion, which represents that the contaminant released amounts to more than $50\%$ of the total amount of the contaminant that can be leached. The amounts of three diesel compounds leached from the specimen in the order of decreasing amount were undecane, pentadecane, and docosane. The curing of the soil contaminated with pentadecane was relatively slow.

A Study of Warm-Mix Asphalt's Bonding Properties on the Change of Asphalt Film Thicknesses (아스팔트 피막두께 변화에 따른 중온 아스팔트의 접착성질에 관한 연구)

  • Yoo, In-Sang;Cho, Dong-Woo;Hwang, Sung-Do;Rhee, Suk-Keun
    • International Journal of Highway Engineering
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    • v.13 no.4
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    • pp.29-40
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    • 2011
  • Warm-Mix Asphalt(WMA) technologies have been developed since 15 years ago, which are internationally and domestically concerned with energy-saving and environmental-friendly technologies in public and private sectors of highway engineering. The performance of asphalt mass is traditionally evaluated by the penetration, viscosity, or Superpave tests. This research, however, is focused more on the properties depending on asphalt film thicknesses instead of evaluating asphalt mass behaviors by those tests. For this approach, a new testing protocol and analysis method are described by the bonding properties on each film thickness. This testing method and analysis tool are borrowed from those of DSR Moisture Damage test and applied by using ARES. The analysis results indicate that there is a limit film thickness between $200{\mu}m$ and $400{\mu}m$, which causes significant changes of the properties. In addition, the results show that the property changes of Hot-Mix Asphalt(HMA) and WMA on the limit film thickness are also different. Therefore, it is suggested that the properties on thin film thicknesses between $200{\mu}m$ and $400{\mu}m$ should be considered in order to evaluate WMA properly.

Effect of Materials and Construction Conditions on Shotcrete Quality (숏콘크리트 품질에 미치는 재료 및 시공 조건의 영향)

  • 현석훈;한기석
    • Proceedings of the Korea Concrete Institute Conference
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    • 1994.04a
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    • pp.227-232
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    • 1994
  • Recently, TBM (Tunnel Boring Machince) method for a tunnel construction in domestic is very promisible due to shorten a constrution period. It is very important to increase the efficiency of the shotcrete for the TBM. The major factors influencing the efficienty of shotcrete are materials, mix disign, constrution conditions and skill of nozzle-man. In this paper, first, optimum synthesize conditions for the shotcrete accelerators was explored and early stiffenting mechanisms also studied. Second, TBM method was applied for a real job site using the optimum conditions obtained from a lab scale experiment.

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A Case Study on the Application of Decomposition Principle to a Linear Programming Problem (분할기법(分割技法)을 이용한 선형계획법(線型計劃法)의 응용(應用)에 관한 사례 연구(事例 硏究))

  • Yun, In-Jung;Kim, Seong-In
    • IE interfaces
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    • v.1 no.1
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    • pp.1-7
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    • 1988
  • This paper discusses the applicability of the decomposition principle to an LP (Linear Programming) problem. Through a case study on product mix problems in a chemical process of Korean Steel Chemical Co., Ltd., the decomposition algorithm, LP Simplex method and a modified method are compared and evaluated in computation time and number of iterations.

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Characteristics of Fatigue Resistance of Recycled Asphalt Concretes by Modified Mixing Process (재생 아스팔트 콘크리트 혼합물의 혼합방법 개선에 따른 피로저항 특성)

  • Doh, Young-Soo;Ko, Tae-Young;Kim, Kwang-Woo
    • International Journal of Highway Engineering
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    • v.8 no.4 s.30
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    • pp.135-143
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    • 2006
  • This study evaluated the fatigue resistance characteristics of hot-mix recycled asphalt mixtures which were prepared by a new blending method. Since the binder of RAP shows higher viscosity without being rejuvenated in the ordinary recycled mixture, this new(modified) blending method was developed for obtaining more uniform level of binder viscosity in the recycled mixture. Cold-planned RAP was collected and mix design was performed using 15% RAP content for two virgin aggregate, gneiss and granite. Penetration grade of 60-80 asphalt was used in mixing recycled mixture together with a polymer modifier, LDPE. Indirect tensile fatigue tests were carried out to evaluate characteristics of fatigue resistance of performance of recycled asphalt mixtures. The recycled mixtures with LDPE 6% showed higher repeated loading fatigue life. Fatigue life ratio of new(N) mixing method mixtures was approximately 0.6-0.7 before aging and 0.8-1.0 after aging treatment of ordinary(O) mixing method mixture. This means the N mixture becomes stronger with aging process increase. If further aging is treated, N mixture may be showing stronger resistance than O mixture.

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Effect of Biochar Treatment on the Growth Characteristics of Q. variabilis for the Restoration of Post-fire Forest Areas (산불피해지 복원을 위한 바이오차 처리가 굴참나무 묘목 생육에 미치는 영향)

  • Yu Gyeong Jung;So Jin Kim;Ju Eun Kim;Jeong Hyeon Bae;Won Seok Kang;Young Geun Lee;Ki Hyung Park
    • Journal of Korean Society of Forest Science
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    • v.112 no.3
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    • pp.290-302
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    • 2023
  • The aim of this study was to examine the changes in the initial tree growth characteristics of Q. variabilis planted in forest fire-damaged areas treated with soil moisturizers such as biochar and to identify the factors that affect tree growth. To evaluate the effectiveness of soil moisture treatment in the area planted with QV, an experimental plot was created according to the treatment method (spread and mix) and treatment ratio (0, 4, and 40 t/ha). The survival rate of QV was 20% higher in the treatment plot than in the control plot. The height and root collar diameter (RCD)growths of QV were high on the northern slope with spread treatment and on the southern slope with mix treatment. The relative growth rate (RGR) according to the soil moisturizer treatment method was higher in the mix treatment, showing a significant difference in RCD. The northern slope had a higher RGR and significant growth rate. This suggests that the growth improvement effect may depend on the soil moisturizer treatment method. The aspect or treatment method affectsthe drying conditions of the soil, which in turn affects its moisture content or nutrient dynamics. The present research results can be used to establish soil moisturizer treatment standards that are suitable for growth purposes. In addition, this study demonstrates that biochar treatment can be considered as an effective alternative to boost biomass and facilitate early restoration of forest fire-damaged areas.

A Study on Aggregate Mix Design of Dumbbell-shape Fiber Reinforced Asphalt Concrete Mixture using Bailey Method (베일리 방법을 이용한 아령형 섬유보강 아스팔트 혼합물의 골재 배합설계법 연구)

  • Ham, Sang-Min
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.14 no.12
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    • pp.6534-6541
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    • 2013
  • The aim of this study was to develop a fiber-reinforced asphalt mixture that was designed to do the following: 1) address fatigue cracks, which is a major source of damage; and 2) increase the rutting resistance. This study reports the effects of the aggregate mixture design that incorporates a dumbbell-shaped fiber. An experiment was carried out to measure the unit weights and unit weight ratios between the mixture that was compacted and the one that was not. A method to substitute a specific aggregate mixture with the dumbbell-shaped fiber was confirmed using the volume concept according to the Bailey method. The results showed that the weight of the PCS aggregate mixture that need to be replaced was 11.88g when a 0.3% reinforcing fiber was added to the 1950g mixture.

Preferences of Mixed Types in a Social Mix Apartment Community and It's Residents' Social Behavior - Focused on the first district in Eunpyeong New Town in Seoul - (사회적 혼합아파트 거주자의 혼합유형 선호특성과 사회적 행태 연구 -서울시 은평뉴타운을 대상으로-)

  • Lee, Hye-Jin;Lee, Yeun-Sook;Lee, Soo-Jin
    • KIEAE Journal
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    • v.11 no.5
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    • pp.69-77
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    • 2011
  • In order to solve social exclusion and segregation phenomenon and to realize social integration, the necessity of social combination and consensus has been presented and existing conception about the public lease apartment confronts a turning point due to the diversification of apartment supply system. With this social background, Eunpyeong New Town Project implemented by Seoul Metropolitan Government is the first example that supplied diverse apartments for social integration. Survey was made upon the residents aged 20 and over and living in combination apartments, lease apartments and common apartments for private ownership in the Enpyeong New Town Apartment Complex in section 1 area, and the survey sheets filled up by the respondents were collected by personal visits or by using specified gathering spots. Summary of study results is as follows: Firstly, regarding the combination method, respondents preferred the irregular combination of lease apartment units and common apartment units in the same apartment building at the top, analyzing by survey groups marked that residents of common apartment with initial ownership responded not to choose combination apartment complex or combination apartment in future. Secondly, the living consciousness with neighbors revealed that respondents were generally satisfied without difference between the residents of lease apartment and common apartment. As the demand and policies for diverse lease apartments are increasing recently, Eunpyeong New Town Project aimed for positive social integration and consensus will be evaluated as model case for social combination apartment in future. And it is necessary to introduce the policies that consider the differences in consciousness and interest of the residents among the lease apartments and common apartments.

Improvement and Expected Effect of Construction Supervision System for Mix-Use Residential Building (주상복합건물의 감리제도 개선방안과 기대효과)

  • Son, Bo-Sik;Bu, Seung-Hyon;Kim, Kyung-Whal;Lee, Hyun-Soo;Ryu, Han-Guk
    • Korean Journal of Construction Engineering and Management
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    • v.7 no.2 s.30
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    • pp.62-70
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    • 2006
  • As buildings are changing variously from the side of use, efficiency and size, the construction qualities are more importantly recognized. Supervision system was enacted in the Residential Building Law in 1990 and responsibility supervision system in the Construction Engineering Management Law in 1994, respectively. However, recently revised supervision system in 2003 has some limitations: the present problems of appointing supervisor, disposing the supervisors, supervisor's works. Therefore, the aim of this study is to improve the present supervision system for mix-use residential building. By implementing the proposed revised supervision system, it will be able to expect quality improvement, confidence of the owner and the user, development of construction technology, and increase international competitiveness of supervision company.