• Title/Summary/Keyword: Missing cells

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Recognition of Missing and Bad Seedings via Color Image Precessing (칼라 영상처리에 의한 결주 및 불량모 인식)

  • 손재룡;강창호;한길수;정성림;권기영
    • Journal of Biosystems Engineering
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    • v.26 no.3
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    • pp.253-262
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    • 2001
  • This study was conducted to develop the vision system of a robotic transplanter for plug-seedling. A color image processing algorithm was developed to identify and locate empty cells and bad plants in the seedling tray. The image of pepper and tomato seedling tray was segmented into regions of plants, frame and soil using threshold technique which utilized Q of YIQ for finding leaves and H of HSI for finding frame of tray in the color coordinate system. The recognition system was able to successfully identify empty cells and bad seeding and locate their two-dimensional locations. The overall success rate of the recognition system was about 99%.

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Developmental Changes of Blastema during Earthworm Tail Regeneration (지렁이 꼬리재생시 재생아의 형태발생에 관한 연구)

  • 조성진;이명식;허소영;신명주;박순철
    • The Korean Journal of Soil Zoology
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    • v.6 no.1_2
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    • pp.1-6
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    • 2001
  • Although the earthworm is an important animal species capable of regenerating missing body part, earthworm regeneration is not well understood at the tissue, cell and molecular levels. In order to understand the developmental changes of blastema during earthworm tail regeneration, the formation and development blastema during regeneration was investigated by histological analysis. Within 1 day after amputation, dediffentiating blastemal cells appeared in coelomic side of longitudinal muscle layer. At 3 days postamputation, proliferating blastemal cells migrated into coelum and blastema was formed beneath wound epithelium around 7 days after amputation. Segmentation of blastema was observed around 2 weeks after amputation followed by redifferentiation of nephridium, intestine, setae and septa.

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Ultrastructure of Fresh Root Turned into Inside White of Red Ginseng (홍삼 내백부의 수삼 미세구조)

  • Cho, Byung-Goo;Park, Hoon;Lee, Jun-Seong
    • Journal of Ginseng Research
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    • v.18 no.3
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    • pp.187-190
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    • 1994
  • The pith and xylem parts of fresh root that turned into inside-white during processing for red ginseng was investigated under scanning electron microscope in comparison with the sa31e position of fresh root processed into normal reddening. In the inside-white part starch storage cells remain mostly in vacancy or with small number of starch granules and with large hollow by missing cell membranes between cells. Many starch seed granules appeared on the surface of storage cell wall in the inside-white part. Fresh root sample showed better picture than dried powder.

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Ultrastructural Changes in Enterocyte infected with Porcine Epidemic Diarrhea Virus (돼지 유행성 설사증 (Porcine Epidemic Diarrhea) 감염 소장상피세포의 전자현미경 관찰)

  • Park, Nam-Yong;Koo, Kyoung-bon;Kim, Seung-Jae;Chung, Chi-Young;Park, Young-Seok;Cho, Kyoung-Oh;Kim, Yong-Hwan;Im, Hyung-Ho
    • Korean Journal of Veterinary Pathology
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    • v.3 no.1
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    • pp.35-44
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    • 1999
  • The morphologic changes of small intestinal epithelium in pigs diagnosed as porcine epidemic diarrhea(PED} by virus isolation and immunohistochemistry were studied through light microscope and transmissible electron microscope. On semi-thin section, the histologic findings showed severe villous atrophy and fusion with hyperplasia of cuboidal epithelium in the villi, inflammatory cell infiltration in lamina propria, and increased mitotic figures in the crypt. The structural changes were mostly restricted to the cytoplasm of affected absorptive epithelium of villi. 3 types of epithelial changes were found; degenerated virus-affected cells, undifferentiated cuboidal cells, and normal columnar cells. On electron microscopy, round to spherical viral particles of 50∼l00nm in diameter were found within the dilated vesicles and endoplasmic reticulums of degenerated cells, which had decreased their cytoplasmic electron density due to dilated and missing organelles(e.g. mitochondria, ERs, etc.). Microvilli were shortened and sparse, leaving denuded terminal web of the villous epithelial cells. Fat globules were often found within slightly degenerated enterocytes. On the tip of villi, severely damaged cells were exfoliated and replaced by undifferentiated cuboidal cells We found distinct ultrastructural changes in the jejunal epithelium confirming PED virus infection is involved in malabsorptive diarrhea.

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COMPOUND ODONTOMA WITH CONGENITAL MISSING OF THE PERMANENT TEETH: CASE REPORTS (영구치의 선천적 결손을 동반한 복합 치아종)

  • Kim, Ha-Na;Kim, Jae-Gon;Baik, Byeong-Ju;Han, Ji-Hye;Yang, Yeon-Mi
    • Journal of the korean academy of Pediatric Dentistry
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    • v.34 no.4
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    • pp.679-684
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    • 2007
  • Odontoma is the most common benign odontogenic tumors, and have been defined as mixed odontogenic tumor composed of epithelial and mesenchymal cells. Odontoma is believed to be hamartomatous rather than neoplastic in nature. The classification by WHO divides odontoma into 2 groups such as complex odontoma and compound odontoma. Compound odontoma comprises dental tissues, resembling the morphology of a tooth and has predilection for the anterior maxilla. In contrast, complex odontoma has unorganized mass, not resembling the normal tooth and has predilection for the posterior mandible. Odontoma is almost asymptomatic, so it is usually found on routine radiographic examination. Common presenting symptom is impacted or unerupted permanent teeth and retained primary teeth, but coexistent odontoma and congenital missing of permanent teeth is a very rare condition. The recommended treatment for an odontoma is conservative surgical excision, with care taken to remove the surrounding soft tissue. This report presents 2 patients with compound odontoma of the mandible who have congenital missing of the permanent teeth.

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Ginsenosides attenuate bioenergetics and morphology of mitochondria in cultured PC12 cells under the insult of amyloid beta-peptide

  • Kwan, Kenneth Kin Leung;Yun, Huang;Dong, Tina Ting Xia;Tsim, Karl Wah Keung
    • Journal of Ginseng Research
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    • v.45 no.4
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    • pp.473-481
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    • 2021
  • Background: Mitochondrial dysfunction is one of the significant reasons for Alzheimer's disease (AD). Ginsenosides, natural molecules extracted from Panax ginseng, have been demonstrated to exert essential neuroprotective functions, which can ascribe to its anti-oxidative effect, enhancing central metabolism and improving mitochondrial function. However, a comprehensive analysis of cellular mitochondrial bioenergetics after ginsenoside treatment under Aβ-oxidative stress is missing. Methods: The antioxidant activities of ginsenoside Rb1, Rd, Re, Rg1 were compared by measuring the cell survival and reactive oxygen species (ROS) formation. Next, the protective effects of ginsenosides of mitochondrial bioenergetics were examined by measuring oxygen consumption rate (OCR) in PC12 cells under Aβ-oxidative stress with an extracellular flux analyzer. Meanwhile, mitochondrial membrane potential (MMP) and mitochondrial dynamics were evaluated by confocal laser scanning microscopy. Results: Ginsenoside Rg1 possessed the strongest anti-oxidative property, and which therefore provided the best protective function to PC12 cells under the Aβ oxidative stress by increasing ATP production to 3 folds, spare capacity to 2 folds, maximal respiration to 2 folds and non-mitochondrial respiration to 1.5 folds, as compared to Aβ cell model. Furthermore, ginsenoside Rg1 enhanced MMP and mitochondrial interconnectivity, and simultaneously reduced mitochondrial circularity. Conclusion: In the present study, these results demonstrated that ginsenoside Rg1 could be the best natural compound, as compared with other ginsenosides, by modulating the OCR of cultured PC12 cells during oxidative phosphorylation, in regulating MMP and in improving mitochondria dynamics under Aβ-induced oxidative stress.

Molecular Approaches to Determine the Character of Serratia marcescens Associated with the Insect Pathogenicity to Brown Planthopper (Serratia marcescens의 곤충 병원성 관련형질 탐색을 위한 분자생물학적 연구)

  • 김희규;배동원;박진희;윤한대
    • Korean journal of applied entomology
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    • v.32 no.3
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    • pp.330-337
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    • 1993
  • A bacterium, pathogenic to Nilaparvata lugens Stal. causing high mortality in 3~5 days, were selected and identified as Serratuz marcescens biotype A2a which is not a nosocomlally infective strain. In order to determine the characters of Serratia marcesce'1lS associated with insect pathogenicity, Tn5 mutagenesis was carried out by conjugating with E. coli pJB4J1. Transconjugants were plate-assayed for missing chitinase, protease and DNase activity. A protease negative mutant was selected for missing JOseet pathogenicity. SEM and TEM revealed the presence of bacterial cells in the epithelial tissue of inner abdomal tissue of the hypodermic layer of abdomen. Such a colonization was limmited to the subjacent tissue inside the intacL cuticular epidermis. These observation supported our result of pathogenicity tests of transconjugants.

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Combination of Brain Cancer with Hybrid K-NN Algorithm using Statistical of Cerebrospinal Fluid (CSF) Surgery

  • Saeed, Soobia;Abdullah, Afnizanfaizal;Jhanjhi, NZ
    • International Journal of Computer Science & Network Security
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    • v.21 no.2
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    • pp.120-130
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    • 2021
  • The spinal cord or CSF surgery is a very complex process. It requires continuous pre and post-surgery evaluation to have a better ability to diagnose the disease. To detect automatically the suspected areas of tumors and symptoms of CSF leakage during the development of the tumor inside of the brain. We propose a new method based on using computer software that generates statistical results through data gathered during surgeries and operations. We performed statistical computation and data collection through the Google Source for the UK National Cancer Database. The purpose of this study is to address the above problems related to the accuracy of missing hybrid KNN values and finding the distance of tumor in terms of brain cancer or CSF images. This research aims to create a framework that can classify the damaged area of cancer or tumors using high-dimensional image segmentation and Laplace transformation method. A high-dimensional image segmentation method is implemented by software modelling techniques with measures the width, percentage, and size of cells within the brain, as well as enhance the efficiency of the hybrid KNN algorithm and Laplace transformation make it deal the non-zero values in terms of missing values form with the using of Frobenius Matrix for deal the space into non-zero values. Our proposed algorithm takes the longest values of KNN (K = 1-100), which is successfully demonstrated in a 4-dimensional modulation method that monitors the lighting field that can be used in the field of light emission. Conclusion: This approach dramatically improves the efficiency of hybrid KNN method and the detection of tumor region using 4-D segmentation method. The simulation results verified the performance of the proposed method is improved by 92% sensitivity of 60% specificity and 70.50% accuracy respectively.

Evaluation of prognosis and cause of failure in 182 cases of autogenous tooth transplantation

  • Jung, Jae-Yong;Kim, Eui-Seong;Lee, Seung-Jong
    • Proceedings of the KACD Conference
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    • 2003.11a
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    • pp.622-622
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    • 2003
  • Autotransplantation is a viable option for the treatment of a missing tooth or for replacement of avulsed and traumatized tooth when there is a donor tooth available. The maintenance of healthy periodontal ligament cells in the donor tooth is one of the most important factors for successful tooth transplantation. The purpose of this study was to evaluate the prognosis and the cause of failure in 182 auto-transplanted teeth. A total of 182 cases which received the auto-transplantation in Department of Conservative dentistry, Yonsei Dental Hospital were included in this study.(omitted)

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Dental anomalies associated with Axenfeld-Rieger syndrome (Axenfeld-Rieger 증후군과 연관된 치과적 이상)

  • Kim, Ki-Rim;Lee, Doo-Young;Kim, Seung-Hye;Lee, Sang-Hui;Choi, Byung-Jai;Lee, Jae-Ho
    • The Journal of Korea Assosiation for Disability and Oral Health
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    • v.6 no.2
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    • pp.94-98
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    • 2010
  • Axenfeld-Rieger syndrome is a rare autosomal dominant disorder characterized by various ocular and extraocular malformations. The incidence of Axenfeld-Rieger syndrome has been estimated to be 1 per 200,000. The syndrome is characterized by short body stature, delayed bone age, and deficient or arrested development of neural crest cells involving the anterior chamber of the eyes, facial bones, teeth, periumbilical skin, and cardiovascular system. This is a case of a 6 year-old girl, who came to the department of the pediatric dentistry, Yonsei University Dental Hospital, for evaluation and treatment of multiple congenital missing permanent teeth. The patient presented typical dental, craniofacial, and systemic features of Axenfeld-Rieger syndrome, such as glaucoma, oval pupil, heterochromatic iris, umbilical hernia, and delayed bone age. On the panoramic view, 3 primary teeth were missing and 13 permanent tooth germs were absent. On the lateral cephalograph, underdevelopment of the maxilla and normal growth pattern of the mandible were confirmed. Periodic dental follow-up is planned for evaluation and interceptive treatment of her dental and craniofacial problems. Denture or removable space maintainer with a pontic is considered for esthetic and functional restoration. In addition, orthognathic surgery is also planned in future to prevent further midfacial skeletal deformation. Early diagnosis of Axenfeld-Rieger syndrome is very important to prevent exacerbation of complications, such as glaucoma and skeletal deformities.

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