• Asian-Australasian Journal of Animal Sciences
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• 제23권10호
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• pp.1261-1267
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• 2010

Milk production traits are important economic traits for dairy cattle. The aim of the present study was to refine the position of previously detected quantitative trait loci (QTL) on bovine chromosome 6 affecting milk production traits in Chinese Holstein dairy cattle. A daughter design with 918 daughters from 8 elite sire families and 14 markers spanning the previously identified QTL region were used in the analysis. We employed a combined linkage and linkage disequilibrium analysis (LDLA) approach with two options for calculating the IBD probabilities, one was based on haplotypes of all 14 markers (named Method 1) and the other based on haplotypes with sliding windows of 5 markers (named Method 2). For milk fat yield, the two methods revealed a highly significant QTL located within a 6.5 cM interval (Method 1) and a 4.0 cM interval (Method 2), respectively. For milk protein yield, a highly significant QTL was detected within a 3.0 cM interval (Method 1) or a 2.5 cM interval (Method 2). These results confirmed the findings of our previous study and other studies, and greatly narrowed down the QTL positions.

• 과학과기술
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• 제23권2호통권249호
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• pp.79-83
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• 1990

• 과학과기술
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• 제23권1호통권248호
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• pp.79-83
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• 1990

• 과학과기술
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• 제22권4호통권239호
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• pp.51-57
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• 1989

• Food Science and Biotechnology
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• 제18권4호
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• pp.823-832
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• 2009

In recent years, special concerns have been raised about the safety assessment of foods and food ingredients derived from genetically modified organisms (GMOs). A growing number of countries establish regulations and laws for GMOs in order to allow consumers an informed choice. In this case, a lot of methods have been developed for the detection of GMOs. However, the reproducibility among methods and laboratories is still a problem. Consequently, it is still in great demand for more effective methods. In comparison with the gel electrophoresis, the capillary electrophoresis (CE) technology has some unique advantages, such as high resolution efficiency and less time consumption. Therefore, some CE-based methods have been developed for the detection of GMOs in recent years. All kinds of CE detection methods, such as ultraviolet (UV), laser induced fluorescence (LIF), and chemiluminescence (CL) detection, have been used for GMOs detection. Microchip capillary electrophoresis (MCE) methods have also been used for GMOs detection and they have shown some unique advantages.

• Bulletin of the Korean Chemical Society
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• 제33권1호
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• pp.278-280
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• 2012

• International Journal of Industrial Entomology and Biomaterials
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• 제4권1호
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• pp.57-62
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• 2002

Cationic liposomes complexed with DNA have been extensively utilized for the delivery of reporter or therapeutic genes both in culture and in vivo. We investigated and determined the optimum conditions of a cationic liposome, composed of dimethyldioctadecy-lammonium bromide (DDAB) and dioleoyl phosphati-dylethanolamine UOPE), mediated a reporter plasmid expressing luciferase into insect cell lines (Sf-21 and Bm-N) and silkworm larvae. Together the data demonstrated that Bombyx mori nuclear polyhedrosis virus (BmNPV) genomic DNA (128 kb) was successfully transfected into Bm-5 cells using this liposome. These results suggest that DDAB/DOPE liposome will be useful as delivery agents for gene transfer to insect cells both in vitro and in vivo.

• International Journal of Industrial Entomology and Biomaterials
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• 제2권2호
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• pp.119-122
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• 2001

Whether Bombyx mori nuclear polyhedrosis virus (BmNPV) can be transmitted to offspring, has been a noticeable question for a long time. When fifth instar larvae of the silkworm were orally inoculated with BmNPV dot hybridization and PCR amplification analysis demonstrated that BmNPV was not detected in the eggs laid by BmNPV productively infected female moths. The results indicated that BmNPV could not be transovarially transmitted.

• 대한임상검사과학회지
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• 제53권1호
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• pp.68-80
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• 2021

An uncertified facility is a facility not certified, as defined in the Biological Agents and Toxins Act (BATA) in Singapore, but has met the requirements of the Ministry of Health to possess First and Fifth Schedule biological agents and toxins. This type of facility is also known as a Biosafety Level 2 Plus (BSL 2+) facility. Registration as an uncertified facility or a BSL 2+ facility requires a certain process and procedure to be sought with the Biosafety Branch of the ministry. This review, shares first-hand knowledge on the journey to achieving registration of the authors' facility. The procedure involved considerable preparation, setting up facility requirements, biosafety precautions, procedures and practices, and training and competence of laboratory users. The ministry conducted a thorough onsite facility audit to ensure that the facility requirements and biosafety procedures and practices were in place. It then issued an approval letter of possession for the first-time use of biological agents and registered the laboratory as an uncertified facility. The expectation is that the comprehensive information shared may be of great benefit to other facilities with similar interests.

• 유통과학연구
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• 제18권12호
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• pp.67-77
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• 2020

Purpose: Based on the author's adapted invariant choice, this study is to present the methodology and the calculation of the integral index of the digital component of the quality of life. By analyzing the digital indexes, the study is also to discuss distribution of ICT and the digital quality of life of the population of Kazakhstan and its regions. Research design, data, methodology: In this research, the method of calculation of integral assessment of the indicator was used, which indicates index constructs. The study analyzed objective secondary data for the period 2017-2019, which was the database from official websites of the Committee on Statistics of the Republic of Kazakhstan. Results: The study produced an integral code for assessing digital components of living standards of the population, consisting of five groups sub-indexes. Conclusions: Based on the provided analyses, we can confirm the existence of a significant difference of all the indicators of digital living standards of the population between the two leading cities: Almaty city and Nur-Sultan city. Furthermore we can deduce the differences of the examined indexes for other regions of Kazakhstan. Despite the rapid adoption of digital technologies, Kazakhstan still has significant digital gaps among cities indicating regional differences in the speed of implementation and distribution of digital technologies.