• Fisheries and Aquatic Sciences
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• v.9 no.2
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• pp.97-100
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• 2006

To determine whether the tetracycline resistance genes tet (34), tet (M), and tet (S) can be transferred among bacteria, we used a filter mating experiment allowing intimate cell-cell contact between donor and recipient. The tet(34) gene, conveyed on a chromosome of Vibrio species (No. 6 and SW-42) was not transferred to Escherichia coli JM109, suggesting that it is not transferred among bacterial species. The tet (M) gene was transferred from three Vibrio strains (4-E, SW-18, and SW-38) to E. coli at frequencies of $8.5{\times}10^{-5}\;to\;2.1{\times}10^{-6}$. The tet(S) gene was transferred from Lactococcus garvieae KHS98032 to E. coli at a frequency of $1.8{\times}10^{-6}$. Transconjugated recipients showed increased minimum inhibitory concentrations against oxytetracycline. Although the donors possess the Tn916-Tn1545 transposons, they were not detected in transformed recipients, suggesting that the transfer of tet(M) and tet(S) is mediated by elements or mechanisms. Two ribosomal protect protein genes were also transmissible from marine bacteria to E. coli, suggesting gene hopping among marine, terrestrial, and human environments.

• Proceedings of the Korea Institute of Fire Science and Engineering Conference
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• 2008.11a
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• pp.202-209
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• 2008

Performance based fire engineering design is being adopted around the world as a rationed means of providing efficient and effective fire safety in Building. This development is being supported by the adoption of performance based codes which specify the objective and minimum performance requirements for fire safety traditional design for fire safety which is still practiced in many countries, relies on "prescriptive" codes which specify how a building is to be built, which no statement of objective and little or no opportunity to offer more rational alterative design. It is the aim of this study to investigate and analyze the research direction of structural fire resistance design of RC structures for recommendation of PBD in Korea.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2009.10a
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• pp.318-318
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• 2009

The door stiffness is one of the important factors side impact. Generally, the researches have been conducted on the assembled door module. This study is to analysis the side impact characteristics for automotives door module. The impact characteristics have been determined by door module side impact test machine. To determine the initial, intermediate and peak crush resistances use the plot of load versus displacement and obtain the integral of the applied load with respect to the crush distances specified below for each door tested. The initial crush resistance is the average force required to deform the door through the initial 6 inches of crush. The intermediate crush resistance is the average force required to deform the door through the initial 12 inches of crush. The peak crush resistance will be directly obtained from the plot of load versus displacement since it is the largest force required to deform the door through the entire 18 inches crush distance. The data are used to determine if a specific vehicle or item of automotives equipment meets the minimum performance requirements of the subject Federal Motor Vehicle Safety Standard(FMVSS). FMVSS Static 214, Side impact protection, specifies performance requirements for protection of occupants in side impact crashes.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.13 no.5
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• pp.82-89
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• 2014

In this paper, fire resistance test was carried out to obtain class H-120 thermal insulation of fire dampers according to a hydrocarbon fire conditions. Specimens were fabricated three different types according to the change of the insulation system applied to damper blade and coaming which were measured surface temperature by performing the fire resistance test. As a test result, specimen-1, 2 of an uninsulated damper blade were exceeded thermal insulation acceptance criteria at 21 minutes, 46 minutes respectively, but specimen-3 of an insulated damper blade was satisfied thermal insulation acceptance criteria during 120 minutes. The test results showed that the insulation of the damper blade was an important factor in the fireproof performance of fire dampers concerning the coaming length minimum 500 mm on the unexposed side as specified test standard.

• Journal of the Society of Naval Architects of Korea
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• v.43 no.5 s.149
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• pp.550-559
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• 2006

A design procedure for a ship with minimum total resistance was developed using a numerical optimization method called SQP(Sequential Quadratic Programming) and a CFD technique based on the Rankine source panel method with the nonlinear free surface boundary conditions. During the whole optimization process the geometry of the hull shape was represented based on the NURBS(Non-uniform rational B-spline) technique and the modification of the hull shape was controlled using the Bell-shaped distribution function to keep the fairness of the hull shape before and after the hull modification. The numerical analysis was carried out using 4000TEU container ship in the towing tank facility installed in the Pusan national university to know the validity of the developed algorithm for this study. As the results of the numerical analysis it proved that the resistance of the optimized hull is conspicuously reduced in comparison with the original hull in a wave-making resistance point of view.

• Journal of Ocean Engineering and Technology
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• v.17 no.1
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• pp.55-60
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• 2003

The effect of size and volume fraction of ceramic particles, with sliding velocity on the wear properties were investigated for the metal matrix composites fabricated by the pressureless infiltration process. The metal matrix composites exhibited about 5.5 - 6 times the wear resistance compared with AC8A alloy at high sliding velocity, and by increasing the particle size and decreasing the volume fraction, the wear resistance was improved. The wear resistance of metal matrix composites and AC8A alloy exhibited different aspects. Wear loss of AC8A alloy increased with sliding velocity, linearly : whereas, metal matrix composites indicated more wear loss than AC8A alloy at the slow velocity region. However, a transition point of wear loss was found at the middle velocity region, which shows the minimum wear loss. Further, wear loss at the high velocity region exhibited nearly the same value as the slow velocity region. In terms of wear mechanism, the metal matrix composites generally exhibited abrasive wear at slow to high sliding velocity； however, AC8A alloy showed abrasive wear at low sliding velocity and adhesive and melt wear at high sliding velocity.

• Journal of Microbiology and Biotechnology
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• v.26 no.9
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• pp.1620-1628
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• 2016

In this study, we investigated colistin resistance mechanisms associated with the regulation of the pbgP operon in Klebsiella pneumoniae, using four isogenic pairs of colistin-susceptible strains and their colistin-resistant derivatives and two colistin-resistant clinical isolates. Amino acid sequence alterations of PhoPQ, PmrAB, and MgrB were investigated, and mRNA expression levels of phoQ, pmrB, pmrD, and pbgP were measured using quantitative real-time PCR. The phoQ and pmrB genes were deleted from two colistin-resistant derivatives, 134R and 063R. We found that phoQ, pmrD, and pbgP were significantly upregulated in all colistin-resistant derivatives. However, pmrB was significantly upregulated in only two colistin-resistant derivatives and one clinical strain. pmrB was not overexpressed in the other strains. The minimum inhibitory concentration of colistin was drastically lower in both phoQ- and pmrB-deleted mutants from a colistin-resistant derivative (134R) that was overexpressing phoQ and pmrB. However, colistin susceptibility was restored only in a phoQ-deleted mutant from a colistin-resistant derivative (063R) without overexpression of pmrB. In conclusion, two different regulations of the pbgP operon may associate with the development of colistinresisant K. pneumoniae.

• Journal of Microbiology and Biotechnology
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• v.24 no.6
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• pp.765-770
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• 2014

This study aimed to characterize CTX-M producers of urinary E. coli and K. pneumoniae isolates and to determine the prevalence of plasmid-mediated antimicrobial resistance genes among them. Minimum inhibitory concentrations (MICs) were determined, and PCR and sequencing were performed. Among the 42 (82.3%) E. coli and 24 (77.4%) K. pneumoniae isolates containing $bla_{CTX-M}$, $bla_{CTX-M-14}$ and $bla_{CTX-M-15}$ were detected in 23 and 19 E. coli isolates, respectively, and in 7 and 17 K. pneumoniae isolates, respectively. CTX-M producers of urinary E. coli and K. pneumoniae were resistant to multiple antibiotics and contained other antimicrobial resistance genes. CTX-M-15 producers contained more antimicrobial resistance genes than did CTX-M-14 producers.

• Proceedings of the KIEE Conference
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• 1987.07b
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• pp.1163-1165
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• 1987

A novel temperature difference-to-frequency converter using two resistance temperature detectors (RTDs) has been developed. The resistance difference of two RTDs is converted into its equivalent inductance to form the resonant circuit of the Colpitts oscillator. The conversion sensitivity of $16\;Hz/^{\circ}C$ and the residual nonlinearity less than 2.15% over the temperature difference range from $35^{\circ}C$ to $155^{\circ}C$ are obtained by the prototype converter. The frequency drift of oscillator itself is ${\pm}0.5\;Hz$. Thus, the minimum detectable temperature difference is estimated to be ${\pm}0.013^{\circ}C$. The proposed converter, except for two RTDs, can be fabricated in monolithic IC form.

• 한국정보디스플레이학회:학술대회논문집
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• 2005.07b
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• pp.1317-1319
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• 2005

Bottom contact pentacene organic thin-film transistors are fabricated at three different substrate temperatures, $70^{\circ}C$, $80^{\circ}C$ and $90^{\circ}C$. The maximum effective mobility was obtained at $80^{\circ}C$. The contact resistance was extracted by applying two different methods, TLM method and channel-resistance method, and the value shows the minimum at $80^{\circ}C$, which is thought to be the important reason for the best performance.