• Journal of Microbiology and Biotechnology
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• 제17권11호
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• pp.1782-1788
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• 2007

Cellulosomes in Clostridium cellulovorans are assembled by the interaction between the repeated cohesin domains of a scaffolding protein (CbpA) and the dockerin domain of enzyme components. In this study, we determined the synergistic effects on cellulosic and hemicellulosic substrates by three different recombinant mini-cellulosomes containing either endoglucanase EngB or endoxylanase XynA bound to mini-CbpA with one cohesin domain (mini-CbpAl), two cohesins (mini-CbpA12), or four cohesins (mini-CbpAl234). The assembly of EngB or XynA with mini-CbpA increased the activity against carboxymethyl cellulose, acid-swollen cellulose, Avicel, xylan, and com fiber 1.1-1.8-fold compared with that for the corresponding enzyme alone. A most distinct improvement was shown with com fiber, a natural substrate containing xylan, arabinan, and cellulose. However, there was little difference in activity between the three different mini-cellulosomes when the cellulosomal enzyme concentration was held constant regardless of the copy number of cohesins in the cellulosome. A synergistic effect was observed when the enzyme concentration was increased to be proportional to the number of cohesins in the mini-cellulosome. The highest degree of synergy was observed with mini-CbpAl234 (1.8-fold) and then mini-CbpAl2 (1.3-fold), and the lowest synergy was observed with mini-CbpAl (1.2-fold) when Avicel was used as the substrate. As the copy number of cohesin was increased, there was more synergy. These results indicate that the clustering effect (physical enzyme proximity) of the enzyme within the mini-cellulosome is one of the important factors for efficient degradation of plant cell walls.

• 생물환경조절학회지
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• 제6권3호
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• pp.198-204
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• 1997

감자 소괴동 산을 위해 적합한 양담재배방식을 구명코자 분무경, NFT, perlite 및 scoria 배지비을 이용하여 '대지' 품종을 공시하고 생육과 소엽도의 연성 및 준양에 미치는 영향을 조사한 결과는 다음과 같다. 1. 분무병 방식이 NFT 방식이나 Perlite 및 scoria 배지경에 비해 정식 후 40일과 60일의 지상부 생조이 좋았고, 정식후 90일에는 동윤이 65.4cm, 주당자육가 7.3개로서 가장 많았으며, scoria 배지경은 기윤이 49.5cm, 도상기육 3.2개로서 가장 적었다. 2. 포지장은 분무비에서 30.4cm로 가장 길었고, 주당각지육는 NFT 방식에서 10.5개로 많았다. 3. 정식 후 90일에 주당 괴경수는 분무경에서 67.1개, NFT 62.5개, Perlite 배지경 20.1개, scoria 배지경 18.0개였다. 4. 3g 이상의 괴대 크기 분포는 분무경 72.7％, NFT 62.6％, perlite 배지비 84.1％, scoria 배지경 83.3％로 고협배지경이 높은 분포를 보였으나 주당 괴동수가 적기 때문에 총수양 은 적었다. 5. Perlite 배지경에서 황산된 소괴경은 피일발이이 적고 표피가 매끄러워서 보질의 괴대이 생산되었다.

• Molecules and Cells
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• 제41권12호
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• pp.1072-1080
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• 2018

A plant-specific B3 domain and AP2 domain-containing transcription factor, RAV1 acts as a negative regulator of growth in many plant species and its transcription was down-regulated by BR and ABA. In this study, we found that RAV1-overexpressing transgenic plants showed abnormally developed ovules, resulting in reduced seed size, weight, and number in a silique. Interestingly, the endogenous expression of RAV1 fluctuated during seed development; it remained low during the early stage of seed development and sharply increased in the seed maturation stage. In plants, seed development is a complex process that requires coordinated growth of the embryo, endosperm, and maternal integuments. Among many genes that are associated with endosperm proliferation and embryo development, three genes consisting of SHB1, MINI3, and IKU2 form a small unit positively regulating this process, and their expression was regulated by BR and ABA. Using the floral stage-specific RNAs, we found that the expression of MINI3 and IKU2, the two downstream genes of the SHB1-MINI3-IKU2 cascade in the seed development pathway, were particularly reduced in the RAV1-overexpressing transgenic plants. We further determined that RAV1 directly binds to the promoter of MINI3 and IKU2, resulting in their repression. Direct treatment with brassinolide (BL) improved seed development of RAV1-overexpressing plants, but treatment with ABA severely worsened it. Overall, these results suggest that RAV1 is an additional negative player in the early stages of seed development, during which ABA and BR signaling are coordinated.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 1996년도 춘계학술대회논문
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• pp.101-109
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• 1996

Mini-mill process which is one of the new steel -marking technologies to be able to produce the hot rolled coils by thin slab caster of ISP(In-Line Strip Production) type, will be completed in the Kwangyang Steel Works of POSCO in August, 1996, SEt-Up Model of finishing mill which consists of 5 stands is the most basic and essential in mini-mill plant. Therefore, the simulation program of Finishing-mill Set-Up model were developed in this research , using new temeprature prediction model, roll gap model and rolling physical model. Using the developed FSU program , pass schedules to produce the strips with target strip thickness of 1.8mm, 2.0mm, 2.3mm, 2.7mm an d3.0mm were also determined respectively.

• Journal of information and communication convergence engineering
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• 제1권1호
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• pp.6-11
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• 2003

In general, standard TCP/IP (transmission control protocol-internet protocol), which is called TCP/IP, is using as the communication basis protocol between any collections of networks that is connected. In this paper, using this TCP/IP implementation of remote control system and suitable program for long distance communication is proposed. This system can make system, which basic Ethernet and TCP/IP used system, to mini modeling, so all module that is using here can be used. Therefore, intention of this paper is to reduce expenses, to effective manage for plant and to increase of productivity as linking each plant of several factory to TCP/IP and Ethernet, and then many control plant and manager minimize the needed course.

• 생물환경조절학회지
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• 제27권2호
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• pp.117-124
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• 2018

최근 1인 가족 증가 및 핵가족화로 인해 미니수박에 대한 소비가 증가되고 있다. 따라서 미니수박에 대한 표준재배법을 확립하기 위하여, 재배유형에 따른 재식거리가 미니수박의 생육, 수량성, 과실특성과 라이코펜 및 당 함량 등에 미치는 영향을 조사하였다. 정식 30일 및 90일후 생육은 재배유형별로 재식거리가 클수록 생육이 양호하였으며, 수확기의 후기생육은 포복형 재배보다는 아치형이나 ${\cap}$자형에서 가장 양호하였다. 주당 착과수는 재배유형별로 유의한 차이는 없었으나, 단위면적당 수량은 밀식재배가 가능한 ${\cap}$자형이 아치형과 포복형에 비해 50% 이상 높았으며, 당도가 다소 증가하였다. 'Minimi'의 과실당 종자수는 대과종인 '삼복꿀'과 비슷하였으나, 종자 100립중은 '삼복꿀'의 1/3크기로 작았다. 라이코펜 함량은 '삼복꿀'보다 'Minimi'에서 30% 이상 높았고, 당성분은 유의한 차이가 없었다. 따라서 미니수박의 재배방식은 ${\cap}$자형 지주재배가 포복재배나 아치형에 비해 단위면적당 수량과 품질이 높았으며, 재식거리는 $120{\times}40cm$에서 수광량, 생육, 수량 및 품질 등에서 가장 양호하였다.

• 한국수소및신에너지학회논문집
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• 제18권1호
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• pp.60-66
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• 2007

In terms of the vehicle efficiency, a fuel cell hybrid system has advantages compared to a conventional internal combustion engine and a fuel cell alone-powered system. The efficiency of the fuel cell hybrid vehicle mainly depends on the maximum power of the fuel cell and therefore it is important to decide the design value of the fuel cell maximum power. In this paper, to estimate the performance of the fuel cell hybrid mini-bus in the design phase the simulator based on the models for the fuel cell stack, the electric battery, the fuel cell balance of plant, the controller, and the vehicle itself is proposed. Additionally, the hybrid mini-bus efficiencies with several different fuel cell powers are simulated for a city driving schedule and are compared on another. Consequently, the proposed simulation scheme is useful to determine the best design value of the fuel cell hybrid vehicles.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2020년도 춘계학술대회
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• pp.120-120
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• 2020

Based on the simple sequence repeat (SSR) marker, a Korean wheat core collection were established with 616 wheat accessions. Among them, the SNP genotyping for the entire genome was performed using DNA chip array to clarify the whole genome SNP profiles. Consequently, a total of 35,143 SNPs were found and we re-established a mini-core collection with 247 accessions. Population diversity and phylogenetic analysis revealed genetic diversity and relationships from the mini core set. In addition, genome-wide association study (GWAS) was performed on 19 phenotypic traits; ear type, awn length, culm length, ear length, awn color, seed coat color, culm color, ear color, loading, leaf length, leaf width, seeding stand, cold damage, weight, auricle, plant type, heading stage, maturation period, upright habit, and degree of flag leaf. The GWAS was performed using the fixed and random model circulating probability unification (FarmCPU), which identified 14 to 258 SNP loci related to 19 phenotypic traits. Our study indicates that this Korean wheat mini-core collection is a set of germplasm useful for basic and applied research with the aim of understanding and exploiting the genetic diversity of Korean wheat varieties.

• 한국작물학회지
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• 제48권3호
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• pp.147-151
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• 2003

제주지역에서 상위급 씨감자 가을채종재배 시 폴리에칠렌(PE)멀칭이 감자의 생육 및 수량성에 미치는 효과를 구명하고자 일반종서, 경삽 및 소서 플러그묘를 2001년 8월 20일에 직파 또는 정식한 후 무멀칭, 투명 및 흑색 PE멀칭재배를 하여 얻어진 결과를 요약하면 다음과 같다. 1. 주당 총서수는 멀칭처리에 큰 영향을 받지 않고 4.6개 내외였으나, 종묘종류별로는 직파구에서 5.14개로 가장 많았고 경삽묘 및 소서묘정식구에서는 각각 4.15, 5.15개였다. 2. 총서수량은 무멀칭구에서 21.16톤/ha이었는데, 투명 및 흑색 PE멀칭에 의하여 각각 16, 8% 증수되었고, 경삽묘정식구가 가장 많았으며 다음으로 소서묘, 직파구 순이었다. 3. 평균서중은 무멀칭구에서 평균 72.2g이었고 투명 및 흑색 PE멀칭에 의하여 각각 14, 12%증가되었는데, 멀칭효과는 경삽묘정식구에서 가장 컸었다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2002년도 제9차 국제심포지움 및 추계정기학술발표회
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• pp.24-24
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• 2002

지상부 생육상태는 일본 원시 표준액에 비해 농심 표준액에서 더 좋았으며, T/R 율은 일본 원시 표준액과 농심 표준액에서 유의차가 없었다. 또한 괴경형성시기는 Atlantic 품종이 일본 원시 표준액에 비해 농심 표준액에서 8일 늦었으며, 다른 품종들과 유의차를 보였다. 주당 괴경중량은 품종별, 양액조성별 유의차를 보였으며 Norchip 품종은 농심 표준액에서 주당 590g으로 가장 높았다. 반면 Gemchip 품종은 농심 표준액에 비해 일본 원시 표준액에서 2배 많았다.(중략)