• Journal of Microbiology and Biotechnology
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• v.17 no.11
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• pp.1782-1788
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• 2007

Cellulosomes in Clostridium cellulovorans are assembled by the interaction between the repeated cohesin domains of a scaffolding protein (CbpA) and the dockerin domain of enzyme components. In this study, we determined the synergistic effects on cellulosic and hemicellulosic substrates by three different recombinant mini-cellulosomes containing either endoglucanase EngB or endoxylanase XynA bound to mini-CbpA with one cohesin domain (mini-CbpAl), two cohesins (mini-CbpA12), or four cohesins (mini-CbpAl234). The assembly of EngB or XynA with mini-CbpA increased the activity against carboxymethyl cellulose, acid-swollen cellulose, Avicel, xylan, and com fiber 1.1-1.8-fold compared with that for the corresponding enzyme alone. A most distinct improvement was shown with com fiber, a natural substrate containing xylan, arabinan, and cellulose. However, there was little difference in activity between the three different mini-cellulosomes when the cellulosomal enzyme concentration was held constant regardless of the copy number of cohesins in the cellulosome. A synergistic effect was observed when the enzyme concentration was increased to be proportional to the number of cohesins in the mini-cellulosome. The highest degree of synergy was observed with mini-CbpAl234 (1.8-fold) and then mini-CbpAl2 (1.3-fold), and the lowest synergy was observed with mini-CbpAl (1.2-fold) when Avicel was used as the substrate. As the copy number of cohesin was increased, there was more synergy. These results indicate that the clustering effect (physical enzyme proximity) of the enzyme within the mini-cellulosome is one of the important factors for efficient degradation of plant cell walls.

• Journal of Bio-Environment Control
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• v.6 no.3
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• pp.198-204
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• 1997

This study was conducted to investigate the effect of solution culture systems on growth and mini-tuber yields of hydroponlcally grown potato (Solanum tuberosun L. cv. Dejima). The growth of stem and foliage at 40 and 60 days after planting were better in aeroponics system than the other systems, and stem length and number of stems per plant at 90 days after planting were the greatest as of 65.4 cm, 7.3 in aeroponics and the lowest as of 49.5 cm, 3.2 in scoria media system. Stolon length was the longest as of 30.4 cm in aeroponics at 90 days after planting, and number of stolons was the greatest as of 10.5 in NFT. Number of mini-tubers per plant at 90 days after planting was 67.1, 62. 5, 20.1 and 18.0 in aeroponics, NFT, perlite and scoria media, respectively. The perlite media system made the fewest enlargement of lenticels of mini-tubers. The results indicate that aeroponic system can be used effectively for mini-tuber potato production.

• Molecules and Cells
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• v.41 no.12
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• pp.1072-1080
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• 2018

A plant-specific B3 domain and AP2 domain-containing transcription factor, RAV1 acts as a negative regulator of growth in many plant species and its transcription was down-regulated by BR and ABA. In this study, we found that RAV1-overexpressing transgenic plants showed abnormally developed ovules, resulting in reduced seed size, weight, and number in a silique. Interestingly, the endogenous expression of RAV1 fluctuated during seed development; it remained low during the early stage of seed development and sharply increased in the seed maturation stage. In plants, seed development is a complex process that requires coordinated growth of the embryo, endosperm, and maternal integuments. Among many genes that are associated with endosperm proliferation and embryo development, three genes consisting of SHB1, MINI3, and IKU2 form a small unit positively regulating this process, and their expression was regulated by BR and ABA. Using the floral stage-specific RNAs, we found that the expression of MINI3 and IKU2, the two downstream genes of the SHB1-MINI3-IKU2 cascade in the seed development pathway, were particularly reduced in the RAV1-overexpressing transgenic plants. We further determined that RAV1 directly binds to the promoter of MINI3 and IKU2, resulting in their repression. Direct treatment with brassinolide (BL) improved seed development of RAV1-overexpressing plants, but treatment with ABA severely worsened it. Overall, these results suggest that RAV1 is an additional negative player in the early stages of seed development, during which ABA and BR signaling are coordinated.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 1996.03a
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• pp.101-109
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• 1996

Mini-mill process which is one of the new steel -marking technologies to be able to produce the hot rolled coils by thin slab caster of ISP(In-Line Strip Production) type, will be completed in the Kwangyang Steel Works of POSCO in August, 1996, SEt-Up Model of finishing mill which consists of 5 stands is the most basic and essential in mini-mill plant. Therefore, the simulation program of Finishing-mill Set-Up model were developed in this research , using new temeprature prediction model, roll gap model and rolling physical model. Using the developed FSU program , pass schedules to produce the strips with target strip thickness of 1.8mm, 2.0mm, 2.3mm, 2.7mm an d3.0mm were also determined respectively.

• Journal of information and communication convergence engineering
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• v.1 no.1
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• pp.6-11
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• 2003

In general, standard TCP/IP (transmission control protocol-internet protocol), which is called TCP/IP, is using as the communication basis protocol between any collections of networks that is connected. In this paper, using this TCP/IP implementation of remote control system and suitable program for long distance communication is proposed. This system can make system, which basic Ethernet and TCP/IP used system, to mini modeling, so all module that is using here can be used. Therefore, intention of this paper is to reduce expenses, to effective manage for plant and to increase of productivity as linking each plant of several factory to TCP/IP and Ethernet, and then many control plant and manager minimize the needed course.

• Journal of Bio-Environment Control
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• v.27 no.2
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• pp.117-124
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• 2018

Recently, the interest and consumption of mini watermelon (Citrullus lunatus Thunb.) are increasing due to nuclear family and one person household. However, there's no research for mini watermelon. Therefore, the purpose of the study is to develop standard cultivation method of mini watermelon. The test cultivar is 'Minimi', which is a small-sized fruit, the rootstock is 'Bullojangsaeng', which is a cucurbit line. Grafted plants were transplanted on April 5, 2017, and harvested in early July. Cultivated types were 3 methods, which are ${\cap}-form$, arched, and runner type, and plant spacing were 40, 60, and 80 cm, respectively. The rowth and yield in ${\cap}-form$ was higher than that of the arched and runner types, and the sugar content was higher a bit. The yield per unit area was about 50% higher than ${\cap}-form$, which can be plant density cultivation than that of the runner type. The number of seeds per fruit of 'Minimi' was similar to that of 'Sambokggul', but seed weight was one third lower than that of 'Sambokggul'. The content of lycopene was 30% higher than that of 'Sambokggul' watermelon, and the main sugar content is similar to or slightly less. In conclusion, the ${\cap}-form$ staking cultivation was superior to the runner type or arched cultivation in terms of fruit setting ratio, yield and quality.

• Transactions of the Korean hydrogen and new energy society
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• v.18 no.1
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• pp.60-66
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• 2007

In terms of the vehicle efficiency, a fuel cell hybrid system has advantages compared to a conventional internal combustion engine and a fuel cell alone-powered system. The efficiency of the fuel cell hybrid vehicle mainly depends on the maximum power of the fuel cell and therefore it is important to decide the design value of the fuel cell maximum power. In this paper, to estimate the performance of the fuel cell hybrid mini-bus in the design phase the simulator based on the models for the fuel cell stack, the electric battery, the fuel cell balance of plant, the controller, and the vehicle itself is proposed. Additionally, the hybrid mini-bus efficiencies with several different fuel cell powers are simulated for a city driving schedule and are compared on another. Consequently, the proposed simulation scheme is useful to determine the best design value of the fuel cell hybrid vehicles.

• Proceedings of the Korean Society of Crop Science Conference
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• 2020.06a
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• pp.120-120
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• 2020

Based on the simple sequence repeat (SSR) marker, a Korean wheat core collection were established with 616 wheat accessions. Among them, the SNP genotyping for the entire genome was performed using DNA chip array to clarify the whole genome SNP profiles. Consequently, a total of 35,143 SNPs were found and we re-established a mini-core collection with 247 accessions. Population diversity and phylogenetic analysis revealed genetic diversity and relationships from the mini core set. In addition, genome-wide association study (GWAS) was performed on 19 phenotypic traits; ear type, awn length, culm length, ear length, awn color, seed coat color, culm color, ear color, loading, leaf length, leaf width, seeding stand, cold damage, weight, auricle, plant type, heading stage, maturation period, upright habit, and degree of flag leaf. The GWAS was performed using the fixed and random model circulating probability unification (FarmCPU), which identified 14 to 258 SNP loci related to 19 phenotypic traits. Our study indicates that this Korean wheat mini-core collection is a set of germplasm useful for basic and applied research with the aim of understanding and exploiting the genetic diversity of Korean wheat varieties.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.3
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• pp.147-151
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• 2003

Normal seed tubers and plug seedlings raised from stem cutting and mini-tubers ($10\pm3$ g) were planted or transplanted on August 20,2001 in bare soil or under transparent and black polyethylene film mulches to evaluate the effects of mulch material and seedling type on the growth and tuber yield of fall-grown potato (Solanum tuberosum L. cv. Dejima) in Jeju region. Total tuber number per plant was 4.6 regardless of mulch treatment, and direct planting seed potatoes resulted in the greatest tubers (5.14)/plant and followed by transplanting seedlings raised from cutting and mini-tubers (4.15 and 5.15 tubers/plant). Transparent and black polyethylene film mulches increased total tuber yield by 16 and 8%, respectively, and average tuber weight by 14 and 12%, respectively, compared with no mulch (21.16 t/ha and 72.2 g/tuber). Transplanting plug seedlings raised from cutting resulted in the greatest tuber yield, followed by transplanting plug seedlings raised from mini-tubers and direct planting seed potatoes.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2002.11b
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• pp.24-24
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• 2002

지상부 생육상태는 일본 원시 표준액에 비해 농심 표준액에서 더 좋았으며, T/R 율은 일본 원시 표준액과 농심 표준액에서 유의차가 없었다. 또한 괴경형성시기는 Atlantic 품종이 일본 원시 표준액에 비해 농심 표준액에서 8일 늦었으며, 다른 품종들과 유의차를 보였다. 주당 괴경중량은 품종별, 양액조성별 유의차를 보였으며 Norchip 품종은 농심 표준액에서 주당 590g으로 가장 높았다. 반면 Gemchip 품종은 농심 표준액에 비해 일본 원시 표준액에서 2배 많았다.(중략)