• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.4
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• pp.658-663
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• 2002

The purpose of this study was to investigate the effect of iron supplementation on utilizations of various minerals, Ca, Mg, Na, K, Fe, Cu, Zn. Intakes, serum levels, and excretions of these minerals were analyzed and compared in rats fed diet with 100, 200, 400% of iron requirement (Fe, 2Fe, 4Fe groups) for 6 weeks. The feed intake, body weight gain and feed efficiency ratio were not significantly different among the groups. Serum Fe of 2Fe group was significantly lower than other two groups, and serum Zn of 4Fe group was highest. Mineral intakes were not significantly different among three groups. With iron supplementation, urinary excretions of Ca, Fe, Cu, Zn were elevated, but fecal excretions of minerals were not significantly different. And the daily retentions of Na and Fe were significantly elevated, but apparent absorbabilities of minerals were not significantly different. In summary, according to iron supplementation, mineral absorbabilities were not different but urinary excretions of some minerals were increased. Therefore, it could be suggested that adequate iron intake and well-balanced diet are more desirable than nutrient supplementation for mineral balance.

• Journal of The Korean Society of Grassland and Forage Science
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• v.22 no.1
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• pp.69-76
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• 2002

This pot experiment was conducted to find out the forage productivity and contents of mineral nutrients in an orchardgrass sward as affected by the compost lime, and phosphorus applications on the newly reclaimed and arable pasture soils. This 1st part was concerned with the effect of compost application. The results obtained are summarized as follows: 1. There were considerable differences between the newly reclaimed and arable pasture soils in the soil chemical properties as fellows(newly reclaimed soil ↔ arable pasture soil): poor ↔common in pH and contents of Ca and Mg; Poor ↔ good in content of available P$_2$O$_{5}$, base saturation, and mutual balance of Ca : Mg : K; common ↔ good in K content; good ↔ good in absorption coefficient of P$_2$O$_{5}$; poor ↔ somewhat poor in CEC. The contents of available P$_2$O$_{5}$ in both soils after experiment were relatively higher in the compost block than non-compost block. It was recognized that the compost application was resulted in the CEC increase in the newly reclaimed soil. 2. The vigour at early growth of orchardgrass was better in the arable pasture soil then newly reclaimed soil. In the newly reclaimed soil, the vigour at early growth of orchardgrass was remarkedly enhanced by compost application. 3. Under the non-compost application, the orchardgrass yield was higher in the arable pasture soil by 50.9% than newly reclaimed soil. Compared with the non-compost application, the orchardgrass yields were increased in the newly reclaimed soil by 17.1% and the arable pasture soil by 7.6% by the compost application. 4. The concentrations of Ca, Mg, and P in orchardgrass were relatively higher in the arable pasture soil than the newly reclaimed soil, whereas there was no difference in the K concentration. It was recognized that the concentrations of Mg and P in orchardgrass were higher in the compost block than the non-compost block.block.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.5
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• pp.460-470
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• 1997

On an absolute straw diet, the effect of graded levels of green grass supplementation on intake, nutrient digestibility, rumen fermentation pattern and microbial N yield has been studied in cattle. Of the two trials conducted, 16 intact growing bulls of 304 kg weight and 32 months old, randomly allocated to four treatments in a completely randomized design in the 1st trial. While, in the, 2nd trial, four rumen cannulated local bulls of about 60 months old and 400 kg weight were used in a $4{\times}4$ Latin square design with four treatments in four periods. In both the trials, in addition to a mineral mixture, animals were supplemented with graded levels of naturally grown green grass of 0 kg (T1), 2 kg (T2), 4 kg (T3) or 6 kg (T4) to an ad libitum rice straw diet In the 1st trial, measurements were made on intake digestibility, growth rate, N balance and microbial N yield. While in the 2nd trial, in addition to the above parameters (except growth rate), rumen parameters were also studied. All levels of grass supplementation decreased the straw DM intake and increased the substitution rate. The rumen $NH_3-N$ concentration increased with the increase in grass level and ranges from 8-46 mg/l. The rumen pH and the rate and extent of DM degradability of straw were not affeceted by different rumen environments created by different levels of grass inclusion. At 48 h, straw DM degradability were 42, 44, 44 and 43% respectively for 0, 2, 4 and 6 kg grass supplementation daily. The whole gut digestibilities of DM, OM and ADF increased significantly (p < 0.05) only at 6 kg level daily. The microbial N yield was not affected by the levels of grass supplemented. The mean microbial N yield was 10 (SD 3.7) g/kg DOM apparently fermented in the rumen. The estimated minimum N loss and thus the maintenance requirement of tissue protein was 303 mg/kg $W^{0.75}/d$. All the animals lost live weight but 6 kg grass supplementation gave positive energy and N balances. Small amount of green grass supplementation is often recommended for optimization of rumen environment of a straw diet However, under the present experimental condition, no such beneficiary effect observed up to 6 kg (26% of DM intake) level of supplementation.

• Korean Journal of Soil Science and Fertilizer
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• v.32 no.1
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• pp.22-30
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• 1999

This experiment was carried out to determine the forage yield and mineral contents of silage corn(Zea mays L.), and N balance, soil chemical characteristics and nitrate-N level in infiltration water by different types and N rates of liquid manure applied. Seven treatments consisting of chemical fertilizer $200kg\;N;ha^{-1}$, liquid cattle manure 200, 320 and $440kg\;N;ha^{-1}$, liquid swine manure 200, 320 and $440kg\;N;ha^{-1}$ were arranged in a randomized complete block design with three replications. The results obtained at National Livestock Research Institute, RDA, in Suweon from May 1997 to Aug. 1997 are summarized as follows : There were no significant differences in percentage of dry matter among the treatments, but significant dry matter yield differences were found (P<0.05), and also the mean dry matter yield of liquid swine manure plot was higher than that of liquid cattle manure plot. Potassium, calcium and magnesium contents of silage corn increased with increasing liquid manure application rates (P<0.05). Nitrate-N content of silage corn in the liquid cattle manure treatments was not influenced by liquid manure application rates, but that in the liquid swine manure treatments increased with increasing the N rates of liquid manure application. pH and contents of exchangeable canons of the soil after experiment were raised by increasing the amount of liquid manure application. Available $P_2O_5$ content in topsoil(0-10cm) was highest as $340mg\;kg^{-1}$ at the plot of liquid swine manure $440kg\;N;ha^{-1}$. Concentration of nitrate-N in infiltration water increased at the plot of liquid swine manure $440kg\;N;ha^{-1}$. Amount of nitrogen balance increased with increasing liquid manure application rates. Based on the results of this experiment, it is suggested that the mean dry matter yield of liquid swine manure plot was higher than that of liquid cattle manure plot, and the amount of nitrogen balance increased with increasing liquid manure application.

• Molecular & Cellular Toxicology
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• v.3 no.4
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• pp.292-298
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• 2007

Zinc plays indispensable roles in metabolism, including cell growth, apoptosis, proliferation and differentiation. Kidneys are target organs for various regulators of mineral metabolism, and play a key role in zinc balance. To investigate the zinc uptake efficiency, we examined the zinc uptake and accumulation level in vivo and in vitro study. Plasma zinc concentration was peaked out at 1 hr after oral zinc administration. The renal zinc level was peaked out at 12 hr after oral zinc administration, and it was the highest in 40 mg/kg Zn-Asp administrated group in comparison with other groups. In addition, the m-RNA expression level of zinc transporter-1 (ZnT-1), zinc transporter-2 (ZnT-2) and high-affinity L-aspartate transporter (EAAT-3) in Zn-Asp administered group were increased compared with control groups and $ZnSO_4$ group. In order to investigate the intracellular zinc uptake mechanism, we performed the in vitro study by using human embryonic kidney cell line, HEK 293. Intracellular zinc level was peaked out at 3 hr after zinc treatment. In the same way, the mRNA expression level of ZnT-1 and EAAT-3 were increased compared with control group. This study showed that Zn-Asp is effective the zinc uptake into the kidney by increasing the zinc transporter expression.

• Journal of Soil and Groundwater Environment
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• v.13 no.6
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• pp.50-61
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• 2008

In this study, new integrated particle tracking algorithm was developed to reduce the inherent problem of Eulerian- Lagrangian method, or adverse effect of particle tracking error on mass balance error. The new integrated particle tracking algorithm includes numerical method, semi-analytical method, and analytical method which consider both temporal and spatial changes of velocity field during time step. Detail of mathematical derivations is well illustrated and four examples are made to verify through the comparison of the new integrated particle tracking with analytical solution or Runge-Kutta method. Additionally, It was shown that the there is better superiority of the new integrated particle tracking algorithm over other existing particle tracking method such as Lu's method.

• Journal of Nutrition and Health
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• v.28 no.5
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• pp.415-425
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• 1995

This study was performed to investigate the effect of dietary protein level on the metabolic changes of Ca and skeletons in postmenopausal women, using ovariecotomized rats as an animal model. The female rats of 200∼250g were fed either 8%(L) or 50%(H) casein diet for 15 weeks(1st experiment). At 15th week, the rats of each diet group were undergone ovariectomy or sham-operation and they were continued to feed the same experimantal diet for 9 more months(2nd experiment). Ca metabolism, kidney function and bone composition were determined at the end of 1st experiment, 3rd and 9th month of 2nd experiment. After 1st experiment, high protein group showed higher urinary Ca and protein excretion, however, there was no difference in GFR and urinary hydroxyproline excretion. The weights, ash and Ca content of femur, scapular and vertebra tended to be higher in high protein groups which tells that high protein promoted skeletal growth. In 2nd experiment, high protein group showed higher urinary Ca and protein excretion and lower Ca absorption and balance. GFR was not affected by dietary protein and ovariectomy but increased with time, as well as kidney weight which shows the continuous development of kidney at this age of 15 month in rats. There were no difference in urinary hydroxyproline, serum ALP, and PTH among experimental groups. The weights of femur, scapular, 4th vertebra increased with time, showing the skeleton continues to grow at this age in rats. However, Ca contents, Ca/wt, Ca/ash were decreased with time and tended to be lower in high protein group especially in femur. In conclusion, prolonged feeding of high protein diet deteriorated Ca metabolism and induced bone loss as time after menopause is extended.

• Journal of Microbiology and Biotechnology
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• v.25 no.10
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• pp.1687-1696
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• 2015

Supplementation with probiotics can protect against the development of food allergies by modulating the host immune response; however, the mechanisms are not fully understood. The objective of this study was to investigate the allergy-reducing effects of regulatory dendritic cells (regDCs) induced by Lactobacillus paracasei L9 (L9) in β-lactoglobulin (BLG)-sensitized mice. The L9 supplement suppressed the aberrant balance of Th1/Th2 responses to BLG in mice, via upregulation of the CD4+CD25+Foxp3+Treg cell responses. The amount of CD4+CD25+Foxp3+Treg cells in mesenteric lymph nodes increased by 51.85%. Furthermore, administration of L9 significantly induced the expression of CD103 and reduced the maturation status of DCs in mesenteric lymph nodes, Peyer's patches, and spleen. Bone marrow-derived dendritic cells (BM-DCs) were activated by L9 in vitro, with an approximate 1.31-fold and 19.57-fold increase in expression of CD103 in CD11c+DCs and the level of IL-10 production, respectively, while the expression of CD86 did not change significantly. These data demonstrate that L9 reduced the BLG allergic sensitization, likely through regDCs mediated active suppression.

• Journal of Nutrition and Health
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• v.21 no.1
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• pp.23-35
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• 1988

This study was designed to find out the effects of aerobic exercise on lipid and Ca metabolism in seven healthy college women, aged 20 to 22 years. Metabolic studies were conducted before and after a 10-weeks exercise period, during which subjects participated in the planned aerobic dance program every day except Sunday. The initial mean time engaged in the exercise was 28 minutes and it was gradually increased up to 45 minutes around the middle point of the exercise period. During both of metabolic study periods, the subjects ate experimental diets which supplied about 120g of protein and 600mg of Ca daily and during the rest of the experimental period they ate their usual diets. The use of alcoholic beverages and drugs were prohibited. The results were summarized as follows. 1) The effect of exercise on lipid metabolism. (1) Mean body weight decreased significantly after exercise(p<0.01) and it did not show a significant decline in skinfold thickness and total body fat contents. (2) Serum total cholesterol level decreased significantly after exercise(p<0.05) and TG level also tended to be lower than that of pre-exercise period. Exercise did not exert any influence on the level of serum HDL-cholesterol in this study. (3) Exercise did not alter total lipid content in feces and apparent lipid absorption rate. 2) The effect of exercise on Ca and P metabolism. (1) After exercise, focal Ca excretion was slightly reduced, however, urinary Ca excretion was not significantly changed. In the results, a slight increase was shown in body Ca retention after exercise. (2) Exercise tended to increase urinary P excretion, but neither P balance nor fecal excretion was significantly changed after exercise. (3) Bone mineral content was not affected by exercise. In summary, aerobic exercise decreased total cholesterol and TG level in serum and tended to increase body Ca retention. With the results, it can be concluded that the additional physical activities beyond the normal daily life in college women might prevent some degenerative diseases-suchas atherosclerosis and osteoporosis.

• The Korean Journal of Community Living Science
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• v.12 no.2
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• pp.33-45
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• 2001

This study was performed to investigate the degree of different stresses in university students of outer region of Seoul and to search the influence of food habit and nutrient intakes in stress. The data was collected by questionnaire method for self-diagnosed different stresses, dietary habit, and physical status and 24 hours recall for nutrient intakes. The subjects were 282 students attended at the lecture Food and Health. Diet balance was no difference with gender but food intakes were more diverse in female. Nutrient intake of male students was lower in vitamin A and riboflavin, but that of female was low in vitamin A and iron. The university students outer region of Seoul had more stress situation in frustration, deprivation and self-efface than in the stress of the noise and Type-A behavioral stress. Total self-diagnosed dietary habit score was better in the group of no susceptive overload stress than that of the high susceptive group. Same trend of dietary behavior score was in the group of no noise and anxiety-response stress than that in the susceptive groups. The quantity of nutrient intakes was correlated with different kind of stress. Niacin intake was positively related with the stress from life-events, and food induced stress. Thiamin intake was positively related with food induced stress. But the intake of iron, calcium, niacin and riboflavin were negatively related with the stress of noise. Therefore, we could concluded that different kind of stresses were influenced in dietary behavior and vitamin-mineral nutrient intakes. More research would be needed at the aspects of health promotion in the stressful situation of modern society.