• Molecules and Cells
• /
• 제41권2호
• /
• pp.93-102
• /
• 2018

Discoidin domain receptor 1 (DDR1) is involved in tumorigenesis and angiogenesis. However, its role in lymphangiogenesis has been unknown. Here, we tested whether downregulation of DDR1 expression by miR-199a/b can suppress lymphangiogenesis. We also aimed to identify miRNA target site(s) in the 3' untranslated region (UTR) of DDR1. Transfection with miR-199a/b-5p mimics reduced expression of DDR1 and tube formation in primary human dermal lymphatic endothelial cells, whereas miR-199a/b-5p inhibitors showed the opposite effects. Critically, injection of miR-199a/b-5p mimics suppressed DDR1 expression and lymphangiogenesis in a corneal alkali-burn rat model. The three well-conserved seed matched sites for miR-199a/b-5p in the DDR1 3'-UTR were targeted, and miRNA binding to at least two sites was required for DDR1 inhibition. Our data suggest that DDR1 promotes enhanced lymphangiogenesis during eye injury, and miR-199a/b-5p suppresses this activity by inhibiting DDR1 expression. Thus, this miRNA may be useful for the treatment of lymphangiogenesis-related eye diseases.

• International Journal of Oral Biology
• /
• 제43권4호
• /
• pp.223-230
• /
• 2018

Exosomes are Nano-sized lipid vesicles secreted from mammalian cells containing diverse cellular materials such as proteins, lipids, and nucleotides. Multiple lines of evidence indicate that in saliva, exosomes and their contents such as microRNAs (miRNAs) mediate numerous cellular responses upon delivery to recipient cells. The objective of this study was to characterize the different expression profile of exosomal miRNAs in saliva samples, periodically isolated from a single periodontitis patient. Unstimulated saliva was collected from a single patient over time periods for managing periodontitis. MicroRNAs extracted from each phase were investigated for the expression of exosomal miRNAs. Salivary exosomal miRNAs were analyzed using Affymetrix miRNA arrays and prediction of target genes and pathways for its different expression performed using DIANA-mirPath, a web-based, computational tool. Following the delivery of miRNA mimics (hsa-miR-4487, -4532, and -7108-5p) into human gingival fibroblasts, the expression of pro-inflammatory cytokines and activation of the MAPK pathway were evaluated through RT-PCR and western blotting. In each phase, 13 and 43 miRNAs were found to be differently expressed $({\mid}FC{\mid}{\geq}2)$. Among these, hsa-miR-4487 $({\mid}FC{\mid}=9.292005)$ and has-miR-4532 $({\mid}FC{\mid}=18.322697)$ were highly up-regulated in the clinically severe phase, whereas hsa-miR-7108-5p $({\mid}FC{\mid}=12.20601)$ was strongly up-regulated in the clinically mild phase. In addition, the overexpression of miRNA mimics in human gingival fibroblasts resulted in a significant induction of IL-6 mRNA expression and p38 phosphorylation. The findings of this study established alterations in salivary exosomal miRNAs which are dependent on the severity of periodontitis and may act as potential candidates for the treatment of oral inflammatory diseases.

• 한국전자파학회논문지
• /
• 제30권1호
• /
• pp.23-27
• /
• 2019

본 논문에서는 비 침습적, 비 파괴적으로 샘플의 단층구조와 단층의 미세진동을 실시간으로 동시에 영상화하는 마이크로 바이브로 토모그라피(micro vibro tomography: MVT) 시스템 개발 및 미세진동 측정 방법을 제안한다. 제안한 방법은 광 간섭 단층촬영기술(optical coherence tomography: OCT)을 기반으로 하여, 샘플의 위상변이를 이용해 마이크로 단위의 움직임을 측정하는 알고리즘을 적용한 방법이다. 본 실험에서는 고막을 모방한 라텍스 샘플에 2~5 kHz 주파수의 음파를 인가시키고, 음파에 의한 샘플의 미세진동을 제안한 MVT 시스템을 이용하여 측정하였다. 그 결과, 고막 모방 샘플의 단층구조와 미세진동을 동시에 영상화할 수 있었고, 표면부의 미세진동을 MVT 표면 진동 이미지로 프로젝팅하여 고막 모방 샘플의 전체적인 진동 형태를 관찰할 수 있었다.

• International Journal of Control, Automation, and Systems
• /
• 제1권3호
• /
• pp.368-375
• /
• 2003

Data fusion approach is investigated to avoid suction in the left ventricular assist system (LVAS) using a nonpulsatile pump. LVAS requires careful control of pump speed to support the heart while preventing suction in the left ventricle and providing proper cardiac output at adequate perfusion pressure to the body. Since the implanted sensors are usually unreliable for long-term use, a sensorless approach is adopted to detect suction. The pump model is developed to provide the load coefficient as a necessary signal to the data fusion system without the implanted sensors. The load coefficient of the pump mimics the pulsatility property of the actual pump flow and provides more comparable information than the pump flow after suction occurs. Four signals are generated from the load coefficient as inputs to the data fusion system for suction detection and a neural fuzzy method is implemented to construct the data fusion system. The data fusion approach has a good ability to classify suction status and it can also be used to design a controller for LVAS.

• Journal of Electrical Engineering and Technology
• /
• 제1권2호
• /
• pp.200-210
• /
• 2006

This paper proposes the motion generation method in which the movement of the 3-links leg subsystem in constrained to slider-link and a singular posture can be easily avoided. This method is the realization of jumping control moving in a vertical direction, which mimics a cat's behavior. To consider the movement from the point of the constraint mechanical system, a robotics system for realizing the motion will change its configuration according to the position. The effectiveness of the proposed scheme is illustrated by simulation and experimental results.

• 한국태양에너지학회 논문집
• /
• 제32권spc3호
• /
• pp.269-274
• /
• 2012

The research of the biological mimics robot which utilizes the operation of the organism is progressed on the ground, aerial, and underwater robot sector. In the field of flying robot, the research for implementing the wing movement structure of the bird and insect is progressed. The joint structure for the wing movement of the bird is implemented. The operation of the wing is simulated. For this purpose, by using the Matlab/Simulink, the joint structure of the wing is modelled. The joint movement of the wing is tested through the simulation.

• 제어로봇시스템학회:학술대회논문집
• /
• 제어로봇시스템학회 2003년도 ICCAS
• /
• pp.2072-2077
• /
• 2003

Animal-like robots are serving an important role as a linkage between biology and engineering. So, in this paper, we aim to develop a biomimetic microrobot that mimics the locomotion mechanism of a gastropod. This microrobot has 3 DOF (x, y translation and rotation), and has small size, unlimited traveling range, high resolution and low cost. Its movement can be made using propagation wave that is generated by the controllable sinusoidal voltage source and piezoelectric effects. This soft motion that can be generated by propagation wave and piezoelectric mechanism would be useful for the motion on the slippery surface. So we modeled the propagation wave mechanism including piezoelectric effect and friction on the contact surface, and could know the velocity of the microrobot is dependent on the driving frequency, input voltage peak, propagation wavelength and surface friction coefficient. With these results we design the microrobot, and accomplish its fabrication and experimentation. The development of this microrobot shall be aimed to design an autonomous moving actuator like animal. Also it can be used from micromanipulation system technology to biology and medicine.

• 한국세라믹학회지
• /
• 제41권12호
• /
• pp.873-879
• /
• 2004

In all major industries ranging from powder industries and advanced ceramics, to the food and pharmaceutical manufacture powder industries, the main production process is the spray dryers. In this paper, a systematic approach is used and six rules are obtained for the basis of the fuzzy model. A fuzzy model is based on the past behavior of the target system and expected to be able to reproduce the behavior of the target system. The output of the developed fuzzy model shows, graphically and statistically, a high level of face validity. Therefore, it is concluded that the developed fuzzy model mimics the actual process and can be considered, with confidence, as a reliable model to study, analyze, and improve the existing process.

• 대한치과교정학회지
• /
• 제43권4호
• /
• pp.178-185
• /
• 2013

Objective: To standardize the facial soft-tissue characteristics of South Korean adults according to gender by measuring the soft-tissue thickness of young men and women with normal facial profiles by using three-dimensional (3D) reconstructed models. Methods: Computed tomographic images of 22 men aged 20 - 27 years and 18 women aged 20 - 26 years with normal facial profiles were obtained. The hard and soft tissues were three-dimensionally reconstructed by using Mimics software. The soft-tissue thickness was measured from the underlying bony surface at bilateral (frontal eminence, supraorbital, suborbital, inferior malar, lateral orbit, zygomatic arch, supraglenoid, gonion, supraM2, occlusal line, and subM2) and midline (supraglabella, glabella, nasion, rhinion, mid-philtrum, supradentale, infradentale, supramentale, mental eminence, and menton) landmarks. Results: The men showed significantly thicker soft tissue at the supraglabella, nasion, rhinion, mid-philtrum, supradentale, and supraglenoid points. In the women, the soft tissue was significantly thicker at the lateral orbit, inferior malar, and gonion points. Conclusions: The soft-tissue thickness in different facial areas varies according to gender. Orthodontists should use a different therapeutic approach for each gender.

• Imaging Science in Dentistry
• /
• 제35권3호
• /
• pp.175-178
• /
• 2005

Necrotizing sialometaplasia (NS) is a rare, benign, self-limiting lesion, but it mimics carcinoma both clinically and histologically. Authors present a case of NS on the right posterior hard palate in a 16-year-old boy. This case showed underlying erosive bone change on CT images. We supposed this lesion resulted from the local anesthesia for dental treatment. Presented NS is the only one case from approximately 1,500 oral and maxillofacial biopsies $(0.07\%)$ at Chonnam National University Hospital during the period from 1999 to 2004.