• 한국동물위생학회지
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• 제36권4호
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• pp.273-281
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• 2013

This study investigated the effects of the alkaline detergent and acid rinse used for cleaning milking machines on the eight commercially available teatcup liner materials. The sample liners prepared for use in the clean-in-place process were analyzed by ultraviolet spectrophotometer, ion chromatography and liquid chromatography. Among the eight liner materials, the ultraviolet spectra of 3 sample liners were shown to have a similar peak shape after cleaning, but the ultraviolet spectra peak shape of 5 sample liners was noticeably changed. No products were detected by ion chromatography in any of the liner materials used in this study. When the liner materials were only treated with alkaline detergent, some additional peaks were observed using liquid chromatography which indicate the creation of molecular substance and elution from liner materials, however, these peaks disappeared when the liner materials were cleaned with the acid rinse. Therefore, we propose that an acid rinse should be applied, after cleaning the milking machine with the alkaline detergent.

• 한국식품위생안전성학회지
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• 제25권4호
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• pp.346-353
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• 2010

본 연구는 젖소 착유농가를 대상으로 착유 및 집유 후 친환경세제를 사용하여 세척효과에 미치는 영향을 조사하기 위해 수행되었다. 착유환경이 다른 5지역의 농가를 대상으로 총 시험 기간 6주 동안 첫 3주는 기존 관행세제인 알칼리 세제와 산세제를 사용하여 착유기, 냉각기 및 착유기 라이너를 세척하였으며, 처리를 달리하여 3주는 알칼리 세제와 친환경세제를 사용하여 세척 하였다. 또한 착유기 라이너 내부를 멸균된 swab을 이용해 시계방향으로 10회 닦은 후 0.1% peptone수에 보관하였으며, 채취된 모든 샘플은 아이스박스를 이용해 보관 운반하여 잔류세균 및 잔류물질 분석을 실시하였다. 미생물 분포는 모든 지역의 착유농가에서 대장균, 대장균군, 황색포도상구균, 분내 대장균군, 녹농균 및 여시니아균이 발견되지 않았으며, 총균수는 관행세제 사용과 비교하여 차이가 없었다. 따라서 친환경세제의 세척효과는 관행세제와 비교하여 비슷한 세척효과를 나타낸 것으로 조사되었다. 세척수 잔류물질은 안성, 온양 및 천안의 젖소 착유농가의 세척수로 사용한 용수에서 질산성 질소가 기준치를 초과하였으며, 세척수의 잔류물질에서도 높은 농도를 나타냈다. 특히 원유의 특이취와 밀접한 관계가 있는 음이온계면활성제의 농도는 관행세제를 사용한 오수와 남원지역의 세척수에서 기준치보다 높은 농도를 나타냈으며, 친환경세제 사용 처리구에서는 기준치보다 낮은 결과를 나타냈다. 따라서 본 연구에서는 친환경 세제를 젖소 착유농가의 착유기 및 냉각기의 세척제로 사용하였을 때, 관행세제와 비교하여 세척효과가 비슷한 것으로 조사됐다. 또한 세척제 사용뿐만 아니라, 위생적인 착유장비 및 세척에 사용되는 용수 관리가 고품질 원유생산에 중요한 영향을 줄 것으로 생각된다.

• 한국동물위생학회지
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• 제41권2호
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• pp.125-131
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• 2018

This study investigated the effects of the alkaline detergent and acid rinse used for cleaning milking machines on the rubber composition of the newly-developed teat cup liners. The samples prepared for use in the clean-in-place process were analyzed by ultraviolet spectrophotometer, ion chromatography and liquid chromatography. In the absorption spectrum of the first sample solution, the form of absorbance or absorption peak was largely different, compared to the absorption spectrum of alkaline detergent alone, but in the absorption spectra of the second and third sample solutions, the absorbance decreased, which was similar to the absorption spectrum in the pure acid detergent. In the ion chromatogram, two main peaks only, which might be shown by the pure alkaline detergent alone, were measured. In the liquid chromatograms, however, new peaks were observed in addition to the two main peaks caused by the pure alkaline detergent alone, which suggested that various molecular materials were created or eluted from the liner by the reaction with the alkaline detergent, but when washed with the acid detergent, any ion species were not produced. Therefore, we propose that an acid rinse should be applied, after cleaning the milking machine with the alkaline detergent.

• 한국환경보건학회지
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• 제11권2호
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• pp.41-54
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• 1985

This study was conducted for the improvement of milk quality and milk hygiene in public health point of view. Investigation of mastiris infection rate, isolation and identification of causative microorganisms in CMT positive milk, investigation of milk contamination level by measuring the bacteria and soma tic cell counts and investigation of dairy management in farms were performed on 1.605 quarters milk of 434 cows of 20 dairy farms in Gyunggi-area from September 1983 to March 1984. The results were summarized as follows 1. Sixteen (3.7%) of 434 cows were found to be infected with clinical mastiris. 234 (53.9%) of 434 cows and 608(37.9%) of 1, 605 quarters were found to be infected with subclinical mastiris. 2. The causative microorganisms isolated were Staphylococcus aureus (38.3%), Staphylococcus epidermidis(21.0%), Micrococci(13.6%), Streptococcus spp. (12.3%), E. coli (7.4%), Fungus & Yeast (1.6%) and others (5.8%). 3. Total numbers of bacteria were $9.2{\times}10^6$ to $1.21{\times}10^7/ml$(av. $1.805{\times} 10^7/ml$), numbers of coliform bacteria were $4.1{\times} 10^5$ to $9.4{\times} 10^5$/ml(av. $7.05{\times} 10^5$/ml) and somatic cell counts were $4.8{\times} 10^5$ to $1.52{\times} 10^6$ cells/ml (av. $9.5{\times} 10^5$cells/ml) in bulk milk. 4. As comparing with CMT score of +, ++ and +++, somatic cell counts were $3.4{\times} 10^5$ to $1.64{\times} 10^6$ cells/ml (av. $6.41{\times} 10^5$cells/ml), $5.4{\times} 10^5$ to $2.75{\times} 10^6$ cells/ml(av. $1.762{\times} 10^6$cells/ml) and $1.97{\times} 10^6$ to $9.75{\times} 10^6$ cells/ml(av. $7.781{\times} 10^6$cells/ml), respectively. 5. In investigation on dairy management, performance of dry cow therapy, teat dipping after milking, disinfection of milking machine at every milking, replacement of milk liner within 6months and opportunity of acquirement for the mastiris control techniques by dairy education were 65%, 40%, 45%, 55% and 50% in 20 dairy farms, respectively.