• 제목/요약/키워드: Milk protein

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중년기의 식습관 및 기호가 건강상태에 미치는 영향 (Effect of Middle-Aged Food Habit and Preference on Health Status)

  • 황춘선;박모라;양이선
    • 한국식생활문화학회지
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    • 제6권4호
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    • pp.351-367
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    • 1991
  • This research was attempted to investigate the correlation between food habits as well as preferences and health conditions of middle-aged people. 380 people living in Dae-gu, kimch$\breve{o}$n and k$\breve{u}$mr$\breve{u}$ng gun in the 40's or 50's were selected for this research as subjects from July 1 to August 15, 1990. The data analysis was made by way of frequency, percentage, ${\chi}^2-test$ and Pearson correlation using SAS package. The summarized results are as follows. 1. Among the total subjects of this research, 200 people (52.6%) were male and 180 people (47.4%) were female. The regional distribution was like this; 115 people (30.3%) lived in large city, 154 people (40.5%) in small and medium city and 111 people (29.2%) in rural region. 2. The survey on food life attitude on the subjects showed that they had a relatively good attitude, and there were little significant differences (p<.05) between men and women. But there were significant differences (p<.001) between rural region and city. 3. On the preferences for taste, food and cooked food the subjects showed the lowest preferences for processed milk-fat food and the highest for soybean and processed soybean food. 4. The research on health condition 1) 30.8% of the subjects were obesity. And this research showed that the percentage of obesity was higher among men (33.5%) than women (27.8%), and higher in cities (40.4, 34.4%) than rural region (16.2%), (p<.05, p<.01). 2) 90.8% of the subjects showed negative according to Diabetes inspection. 3) 2.0% of the subjects were hypertensive, and the percentage was higher among women than men, and higher in rural region than cities. 4) 12.6% of the subjects were anemia, and the percentage was higher among women (17.8%) than men (8.0%), and higher in rural region (23.4%) than cities (7.0, 9.1%). 5) DMFT index and DMF rate of dental caries was higher among women (DMFT index; 10.6 DMF rate; 88.9) than men (DMFT index; 7.3 DMF rate; 81.5), and higher in rural region (DMFT index; 11.8 DMF rate; 90.1) than cities (DMFT index; 7.4, 7.9 DMF rate; 79.1, 85.7). 6) According to the survey on self-diagnosed health status of subjects, the percentages of articular·neuralgia (48.9%) was the highest. And that of stomach digestion troubles (31.1%), headache (22.4%), anxiety excitement (12.9%), spastic constipation (12.4%), insomnia (9.7%), melancholia (7.9%) and etc (1.6%) followed. 7) People had allergied food which contained animal protein such as pork, chicken, mackerel, siakworm pupa, clam and so on. 8) In female cases, 46.7% of women became already menopausal and 13.3% of them was under menopause. 5. This research showed that there were significant correlation between food life attitude and health condition in obesity, anemia and dental caries but not in blood pressure. 6. And this research also showed that there were significant correlations between food preference and health condition in obesity, anemia and dental caries but not in blood pressure.

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간암세포주에서 상피간엽전환억제를 통한 Silymarin의 침윤 및 전이 억제 효과 (Silymarin Attenuates Invasion and Migration through the Regulation of Epithelial-mesenchymal Transition in Huh7 Cells)

  • 김도훈;박소정;이승연;윤현서;박충무
    • 대한임상검사과학회지
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    • 제50권3호
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    • pp.337-344
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    • 2018
  • 발생하는 간암 중 가장 주요한 형태인 간세포암은 강한 전이특성으로 인해 높은 재발율과 사망률을 보인다. Silymarin은 엉겅퀴에서 추출한 플라보노이드 성분으로 여러 암세포주에서 상피간엽전환(epithelial mesenchymal transition, EMT) 조절을 통해 항암효과를 보이는 것으로 보고되었다. 본 연구에서는 silymarin이 EMT의 조절을 통해 간세포암 세포주인 Huh7 cell의 침윤과 전이를 억제하는지를 분석하고자 하였다. Huh7 cell의 침윤과 전이 활성을 분석하기 위하여 wound healing assay와 in vitro invasion assay를 시행하였고 EMT 관련 유전자와 상위 신호전달물질의 발현 분석을 위해 Western blot assay를 실시하였다. 그 결과 silymarin은 농도 의존적으로 Huh7 cell의 침윤과 전이를 억제하였다. EMT 관련 유전자 중 세포 부착 단백질인 E-cadherin은 증가하였으나, 중간엽세포의 지표인 vimentin, 종양미세환경 조절에 관여하는 MMP-9의 발현은 억제되었고 이들의 활성에 관여하는 전사인자인 Snail과 nuclear factor $(NF)-{\kappa}B$ 또한 농도 의존적으로 활성이 감소하는 것을 확인할 수 있었다. 특히, 상위신호전달물질 중 silymarin은 phosphoinositide-3-kinase (PI3K)/Akt의 인산화 억제를 통해 EMT 관련 유전자들을 조절하는 것으로 나타났고 이것은 selective inhibitor인 LY294002의 처리 결과로 확인할 수 있었다. 결과적으로, silymarin은 PI3K/Akt 경로를 통해 EMT 관련 유전자의 발현을 조절함으로써 Huh7 cell의 침윤과 전이를 억제하는 것으로 생각된다. 이를 통해 silymarin이 간세포암의 전이 억제에 효과적인 항암물질의 후보가 될 수 있는 잠재력을 가진 후보물질이 될 수 있음을 보여주었다.

생육시기 및 품종이 총체 벼의 수량 및 사료가치에 미치는 영향 (Effect of Growth Stage and Variety on the Yield and Quality of Whole Crop Rice)

  • 김종근;정의수;함준상;서성;김맹중;윤세형;임영철
    • 한국초지조사료학회지
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    • 제27권1호
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    • pp.1-8
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    • 2007
  • 본 시험은 사료용 총체 벼의 수확시기 및 품종이 수량 및 사료가치에 미치는 영향을 구명하기 위하여 2003년부터 2005년까지 축산연구소에서 수행되었다. 식용벼인 추청벼와 총체벼 전용품종인 하마사리를 이용하여 생육시기별로 6회(출수, 개화, 유숙, 호숙, 황숙 및 완숙기)에 걸쳐 수확하였다. 조단백질 함량은 수확이 늦어짐에 따라 감소하였으나 TDN 함량은 증가되었다. ADF 함량으로 추정된($TDN=88.9-(0.79{\times}ADF)$) 평균 TDN 함량은 60% 내외로 나타났다. ADF 및 NDF 함량은 수확이 지연됨에 따라 감소되었다. 건물수량은 황숙기에 가장 높았으며(p<0.05) 추청벼가 하마사리 보다 약간 높았지만 유의적이 차이는 없었다. TDN 수량도 건물수량과 같은 경향을 보였으나 매 수확시기별 품종간에는 유의적인 차이가 나타났다(p<0.05). 따라서 이상의 결과를 종합하여 볼 때 총체 벼의 수확적기는 호숙기${\sim}$황숙기로 추천되었다.

수확시기 및 품종이 총체 벼 사일리지의 품질에 미치는 영향 (Effect of Growth Stage and Variety on the Quality of Whole Crop Rice Silage)

  • 김종근;정의수;서성;김맹중;이종경;윤세형;임영철;조용민
    • 한국초지조사료학회지
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    • 제28권1호
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    • pp.29-34
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    • 2008
  • 본 시험은 품종 및 수확시기가 총체 벼 사일리지의 품질에 미치는 영향을 구명하기 위하여 2004년부터 2005년까지 축산연구소에서 수행되었다. 식용벼인 추청벼와 총체 벼 전용품종인 하마사리를 이용하여 생육시기별로 6회(출수기, 개화기, 유숙기, 호숙기, 황숙기 및 완숙기)에 걸쳐 수확을 하여 사일리지로 조제하였다. 조단백질 함량은 숙기가 진행됨에 따라 감소되었으며 TDN 함량은 증가되었다. 평균 조단백질 함량은 총체 벼 전용품종이 식용벼 품종보다는 높게 나타났다. ADF 및 NDF 함량은 숙기가 진행됨에 따라 감소되었으며 ADF로 추정된 TDN 함량은 숙기가 진행됨에 따라 증가되었다. 그러나 품종간에는 통계적 유의성이 나타나지 않았다(p<0.05). 사일리지의 평균 pH는 4.69 이었으며 숙기가 진행됨에 따라 증가하였다. 생육이 진행됨에 따라 초산 및 낙산 함량은 감소되었으나 젖산 함량은 증가되었다. 품질등급은 호숙기에서 가장 우수하였다. 이상의 결과를 종합하여 볼때 총체 벼 사일리지는 전용 품종이 일반 벼 품종보다 약간 개선되는 것으로 나타났으나 유의적인 차이는 없었다. 따라서 양질의 총체 벼 사일리지 조제를 위해서는 호숙기 전후에 수확하여 사일리지를 만드는 것이 추천된다.

Comparison of Carcass Composition of Iranian Fat-tailed Sheep

  • Kiyanzad, M.R.
    • Asian-Australasian Journal of Animal Sciences
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    • 제18권9호
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    • pp.1348-1352
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    • 2005
  • Most breeds of sheep in Iran are adapted to their agro ecological niches where it is likely that they were also artificially selected by their owners. In general, most of sheep breeds are multipurpose producing lambs, wool and milk. To compare the physical and chemical composition of the carcasses of ten Iranian native fat-tailed sheep breeds, 243 male lambs (6-7 months of age) of ten fattailed, Iranian breeds of sheep, Sanjabi (S), Ghezel (G), Afshari (A), Mehrabani (M), Lori (L), Lori Bakhtiari (LB), Kordi Khorasan (K), Sangesari (Sa), Baluchi (B) and Chal (C) were studied. Lamb breed group had a significant (p<0.05) effect on all the carcass traits measured. The LB and C lambs showed the same live weight which was significantly (p<0.05) higher than the other breeds. Carcass dressing- out percentage in S lambs was lowest (p<0.05), but not different from G lambs. K and Sa breeds showed the highest (p<0.05) carcass dressing-out percentage. The S lambs had the highest (p<0.05) lean meat percent. Lean meat percentage was not significantly (p>0.05) different in the G, A, M and C breeds. The Sa and K breeds showed the lowest lean meat percent. The S lambs showed the lowest (p<0.05) fat percent in their carcass, while K and Sa showed the highest (p<0.05). Subcutaneous fat in K, Sa and B was higher (p<0.05) than the other breeds. Lambs of S, G, A and M breeds had the lowest subcutaneous fat in their carcasses (p>0.05). Intramascular fat was significantly (p<0.05) lower in M, S and C despite the fact that this values were highest in B and K lambs. The K and Sa breeds had highest fat-tail percentage (p<0.05) in their carcass, whereas S and G showed lowest. Lambs of G, S and A breeds had higher bone percent than other breeds (p<0.05). Lowest bone percent (p<0.05) was seen in K and Sa lambs. The carcass moisture percent was not significantly (p>0.05) different in S, G, A, M, L and C breeds. M lambs showed the lowest crude protein percentage and S breed the highest (p<0.05). There were no significant (p>0.05) differences among other lamb breeds for this trait. Chemical fat percentage was the same in S, G, A, C and M breeds, but significantly (p<0.05) lower from LB, K, Sa and B. Ash percent in S, G and A had no significant (p>0.05) difference. According to higher lean meat and lower fat percentages in the carcass, the ranking of breeds would be S, G, A, M and C.

Genome-wide DNA methylation pattern in a mouse model reveals two novel genes associated with Staphylococcus aureus mastitis

  • Wang, Di;Wei, Yiyuan;Shi, Liangyu;Khan, Muhammad Zahoor;Fan, Lijun;Wang, Yachun;Yu, Ying
    • Asian-Australasian Journal of Animal Sciences
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    • 제33권2호
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    • pp.203-211
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    • 2020
  • Objective: Staphylococcus aureus (S. aureus) is one of the major microorganisms responsible for subclinical mastitis in dairy cattle. The present study was designed with the aim to explore the DNA methylation patterns using the Fluorescence-labeled methylation-sensitive amplified polymorphism (F-MSAP) techniques in a S. aureus-infected mouse model. Methods: A total of 12 out-bred Institute of Cancer Research female mice ranging from 12 to 13 weeks-old were selected to construct a mastitis model. F-MSAP analysis was carried out to detect fluctuations of DNA methylation between control group and S. aureus mastitis group. Results: Visible changes were observed in white cell counts in milk, percentage of granulocytes, percentage of lymphocytes, CD4+/CD8+ ratio (CD4+/CD8+), and histopathology of mice pre- and post-challenge with S. aureus. These findings showed the suitability of the S. aureus-infected mouse model. A total of 369 fragments was amplified from udder tissue samples from the two groups (S. aureus-infected mastitis group and control group) using eight pairs of selective primers. Results indicated that the methylation level of mastitis mouse group was higher than that in the control group. In addition, NCK-associated protein 5 (Nckap5) and transposon MTD were identified to be differentially methylated through secondary polymerase chain reaction and sequencing in the mastitis group. These observations might play an important role in the development of S. aureus mastitis. Conclusion: Collectively, our study suggests that the methylation modification in Nckap5 and transposon MTD might be considered as epigenetic markers in resistance to S. aureus-infected mastitis and provided a new insight into S. aureus mastitis research in dairy industry and public health.

계절성으로 재발한 특발성 폐 혈철 침착증 1예 (A case of idiopathic pulmonary hemosiderosis with seasonal recurrence)

  • 곽가영;이나영;이문희;이수영;정승연;강진한;정대철
    • Clinical and Experimental Pediatrics
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    • 제52권2호
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    • pp.256-260
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    • 2009
  • 특발성 폐 혈철 침착증은 반복되는 객혈, 흉부 X-선상 양측성 폐침윤, 철분 결핍성 빈혈을 특징으로 하는 질환으로 주로 소아에서 발생한다. 매우 드문 질환으로 국내에서는 현재까지 6편의 증례가 보고되었다. 유전, 자가면역, 알레르기, 환경, 대사 등과 관련된 병인에 제시되었으나 아직까지 확실히 밝혀지지 않았다. 본 환자는 9세 여아로 2년간 매 봄, 가을에 객혈, 호흡곤란을 주소로 4차례 입원하였다. 청진 시 양폐야에서 나음이 들렸고 흉부 X-선 검사 및 CT에서 양측성 폐포성 침윤이 확인되었으며 혈액 검사에서 철분 결핍성 빈혈이 관찰되었다. Anti-GBM Ab, ANCA, FANA, RF, cow's milk protein에 대한 IgE 등의 항체 검사는 모두 음성이었고 응고 검사, 면역글로불린, 보체는 모두 정상이었다. 세균에 대한 객담과 혈액 배양 및 결핵에 대한 피부반응검사, 객담 도말 및 배양 검사는 모두 음성이었다. 폐생검에서 폐포내 출혈과 혈철소 함유 대식 세포(hemosiderin-laden macrophage) 및 간질의 섬유화 소견을 보여 폐 혈철 침착증을 확진 하였다. 매 악화 시마다 경구용 스테로이드 투여 후 임상 양상이 호전되어 퇴원하였다. 현재 외래에서 유지요법으로 스테로이드 복용 중이며 17개월간 재발 없는 상태이다. 저자들은 이전에 국내에서 보고되었던 환자들과는 달리 학동기에 처음 발병하여 매년 봄과 가을에 뚜렷한 원인 없이 재발하는 양상을 보인 특발성 폐 혈철 침착증 1예를 보고하는 바이다.

우리나라 성인의 식사패턴 변화 추세 - 1998, 2001, 2005년도 국민건강영양조사자료를 이용하여 - (Secular Trend in Dietary Patterns in a Korean Adult Population, Using the 1998, 2001, and 2005 Korean National Health and Nutrition Examination Survey)

  • 강민지;정효지;임정현;이연숙;송윤주
    • Journal of Nutrition and Health
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    • 제44권2호
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    • pp.152-161
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    • 2011
  • Koreans have undergone many changes in dietary patterns with economic growth. The purpose of this research was to examine changes in dietary patterns using data from the 1998, 2001, and 2005 Korean National Health and Nutrition Examination Survey. The study included 21,525 subjects (8,295 from 1998, 6,880 from 2001, and 6,350 from 2005) who were 20 years or older and who participated in a 24-h diet recall. The percentage energy intake from 22 food groups was calculated, and a cluster analysis was applied to identify dietary patterns. Two dietary patterns emerged; the first pattern was characterized by high intake of white rice, legumes, vegetables, kimchi, and seaweeds, the so-called "traditional" pattern (53%), whereas the other pattern was characterized by high intake of other grains, noodle dumplings, floured bread, pizza, hamburgers, cereals and snacks, potatoes, sugared sweets, nuts, fruits, meat·its products, eggs, fish, milk and dairy products, oils, beverages and seasoning, or the so-called "modified" pattern. The modified pattern comprised a higher proportion of younger aged, metropolitan residents with more education and higher incomes. However, the gender distribution was not significantly different. The modified pattern had a significantly higher intake of all nutrients except carbohydrates and had a higher proportion of energy from fat and protein. No association with a risk for metabolic syndrome was found for either dietary pattern. After age was standardized, the traditional pattern included 52% of the respondents in 1998, 54% in 2001, and 50% in 2005. However, the modified pattern was significantly more prevalent in the younger age group (20-29 yr), whereas the traditional pattern increased significantly in the older age group (${\geq}$ 65 yr). In conclusion, a secular trend was found for dietary pattern by age group, suggesting that it is necessary to monitor the changes in dietary pattern by age group and to develop appropriate dietary education and guidelines.

한국 성인의 Vitamin D 영양 상태가 골밀도에 미치는 영향 (The Effect of Vitamin D Status on Bone Mineral Density of Korean)

  • 문수재
    • Journal of Nutrition and Health
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    • 제31권1호
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    • pp.46-61
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    • 1998
  • This study was carried out to evaluate the effects of vitamin D nutritional status on bone mineral density of adults (21-49 years). To attain the aim, we measured bone mineral density (BMD) of the subjects at distal radius by single-photon absorptiometry (SPA). Serum level of 25-hydroxyvitamin D(25-(OH)D) , known to be the best indicator of indicator of vitamin D status in humans was analyzed . The factors affecting this vitamin D level were also investigated in autumn in 122 young adults. Serum level of 25-(OH)D was measured by high pressure liquid chromatography(HPLC) and biochemical variables, general health status, time spent outdoors, and dietary intakes of the subjects. BMD of the male subjects was significantly greater than that of female subjects. Weight, activity and total energy expediture (TEE) showed a positive correlation with distal BMD. The mean level of serum 25-(OH)D was 24.4$\pm$11.0 ng./ml and by sex, 26.0$\pm$6.8ng/ml for males and 23.3$\pm$12.3ng/ml for females , the level was significantly higher in male (p<0.01). there was significant correlation between BMD at distal-radius and s-25(OH)D levels (p<0.001). The serum level fo parathyroid hormone (PTH) showed a negative correlation with BMD(p<0.05), with the more obvious correlation in females. Vitamin D intake was estimated to be 3.75$\pm$2.19ug/day in average. Among the nutrients studied, protein ,fat, calcium , and vitamin D intake were positively correlated with distal BMD. When food frequencies were concerned , milk and dairy products showed a significant positive correlation with the BMD level, and driedfoods, eggs , fats and oils, and cereals also showed a positive correlation. Time spent outdoors was estimated to be about 70 minutes in average and positively correlated with the distal BMD level(p<0.01). During the day, the specific time between 12 :00pm and 2:00pm showed the most significant correlation with BMD (p<0.001). Multiple regression analysis with the variables showed that distal BMD could be fit 31.9% by the time spent outdoors a day, intake of Ca and vitamin D, and TEE. The standardized estimates were 0.344 for vitamin D intake, 0.284 for Ca intake 0.179 for the time spent outdoors a day and 0.273 for TEE. For males, s-25*OH)D level, TEE and time spent outdoors during a day showed a significant correlation. For females, intake of Ca and vitamin D could fit about 27.1% of the distal BMD.

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한국 성인 남녀의 채소, 과일 섭취와 천식 유병률의 관련성: 2013-2017 국민건강영양조사 자료를 이용하여 (Association of fruit and vegetable consumption with asthma: based on 2013-2017 Korea National Health and Nutrition Examination Survey)

  • 김은경;주세영
    • Journal of Nutrition and Health
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    • 제53권4호
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    • pp.406-415
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    • 2020
  • 본 연구는 2013-2017년 국민건강영양조사 자료를 이용하여 만 19-64세 성인 남녀를 대상으로 채소·과일 섭취에 따른 일반적 사항과 식품 및 영양소 섭취 상태를 파악하고 채소·과일의 섭취와 천식 유병률의 관련성을 알아보고자 시행되었다. 전체 대상자의 천식 유병률은 2.6% 이었고, 채소·과일의 섭취량에 따라 1.8%-3.1%로 나타났다. 채소·과일 섭취량이 많을수록 평균 연령과 가구소득은 높았고 교육수준은 낮았으며, 흡연자의 비율과 알코올 섭취 빈도도 낮았다. 채소·과일의 섭취량이 많은 군이 우유 및 유제품을 제외한 모든 식품군의 섭취량이 많았고, 에너지를 포함한 영양소의 섭취량도 많았다. 열량영양소의 섭취비율의 경우, 채소·과일의 섭취량이 많은 군이 적은 군에 비해 탄수화물과 단백질의 섭취비율은 높고, 지방의 섭취비율은 낮았다. 채소와 과일 섭취량에 따른 3사분위군의 천식에 대한 위험도는 최하위 사분위군 대비 각각 35%, 40%까지 감소하였다. 그러나 최상위 사분위군의 경우, 최하위 사분위군과 비교하여 천식에 대한 위험도가 유의하게 감소하지 않았다. 이러한 결과를 통하여 권장량 수준의 적절한 채소·과일의 섭취가 천식을 예방 및 관리하는데 도움이 될 것이라 생각된다. 또한 채소·과일의 섭취가 천식의 발생과 치료효과에 미치는 영향을 파악하기 위해 장기간의 코호트 연구와 임상시험 연구가 필요할 것으로 생각된다.