• Korean Journal of Animal Reproduction
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• v.24 no.4
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• pp.321-333
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• 2000

High production of milk and its components are necessary to allow maximal growth of developing piglets. In this study, transgenic pigs were produced containing the $\alpha$ -lactalbumin gene, whose product is a potential limiting component in the production of milk. Two lines of transgenic pigs were produced to analyze the effects that overproduction of the milk protein $\alpha$ -lactalbumin may have on milk production and piglet growth. Transgenic pigs were produced through microinjection of the bovine $\alpha$ -lactalbumin gene. The gene construct contained 2.0 kb of 5'flanking region, the 2.0 kb coding region and 329 bp of 3'flanking region. Sows hemizygous for the trans gene produced as much as 0.9 g of bovine $\alpha$-lactalbumin per liter of pig milk. The production of the bovine protein caused approximately a 50% increase in the total $\alpha$ -lactalbumin concentration in pig milk throughout lactation. The concentration of bovine $\alpha$ -lactalbumin was highest on day 0 and 5 of lactation and decreased as lactation progressed. The ratio of bovine to porcine $\alpha$ -lactalbumin changed during the sow's lactation. This ratio was 4.3 to 1 on day 0 of lactation, but by day 20 of lactation the ratio was 0.43 to 1. This suggested that the bovine transgene and the endogenous porcine gene were under slightly different control mechanisms. The higher level of total $\alpha$-lactalbumin present on day 0 of lactation was correlated with higher lactose percentage on day 0 in transgenic sows (3.8%) as compared to controls (2.6%) (P＜0.01). Although there was also a trend for higher lactose percentage in transgenic sows on day 5 and 10 of lactation, no significant differences were observed. These data suggest that $\alpha$ -lactalbumin is limiting early in lactation of swine. Furthermore, higher concentrations of $\alpha$ -lactalbumin early in lactation may boost milk output.

• Journal of Environmental Health Sciences
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• v.6 no.1
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• pp.57-63
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• 1979

A study on consecutive changes of milk was carried out to determine the changes of milk components and microorganisms by different containers. The containers included in this study were glass bottles, polyethylene packs, polystyrene bottles and aluminum foils. The number of total microorganisms and coliforms, contents of milk fat and vitamin A were tested. The results obtained were as follows: 1) The number of total microorganisms exceeded the legal criteria within 2-3 days at 4$\circ$C, 24 hours at room temperature (20-23$\circ$C) and 3 hours at 35$\circ$C. 2) The number of coliforms in milk contained in glass bottles exceeded the legal criteria within 3 days at 4$\circ$C, that of glass bottles and polyethylene packs within 12 hours at room temperature (20-23$\circ$C), that of polyethylene packs and polystyrene bottles within 6 hours at 35$\circ$C, and that of glass bottles within 9 hours at 35$\circ$C respectively. 3) The milk fat contents by di'fferent containers were not changed at all. 4) Vitamin A contents were reduced by a slight exposure to sunlight. The destruction rates of all containers were the highest in an hour after exposure to sunlight. The rates by different containers were 50.1% in glass bottles, 47.6% in polyethylene packs, 30.4% in polystyrene bottles in an hour after exposure respectively. After an hour exposure, the destruction rates gradually decreased. The vitamin A destruction rate of glass bottles were the highest, and that of polyethylene packs were next, and that of polystyrene bottles were the lowest 5) The changes of pH of milk were high in the following order: the milk in glass bottles, polyethylene packs, and polystyrene bottles. pH of milk in aluminum foils were not changed.

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.1
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• pp.75-85
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• 2012

An experiment was conducted to compare the effect of the same amount of 18:2 offered either as 18:2n-6 or as a mixture of unprotected 18:2c9t11 and 18:2t10c12 on feed intake, milk components as well as plasma and milk fatty acid profile. Fifteen cows were blocked by milk yield and milk fat percentage and within block assigned randomly to 1 of 3 treatments (n = 5). Each cow passed a 12-d adjustment period (AP) on a basal diet. After the AP cows received 1 of 3 supplements during an 18-d experimental period (EP). The supplements contained either 1.0 kg ground sunflower seeds (S), 0.5 kg conjugated linoleic acid (CLA)-oil (C) or 0.75 kg of a mixture of ground sunflower seeds and CLA-oil (2:1; SC). All 3 supplements contained the same amount of 18:2 either as CLA (${\Sigma}18$:2c9t11+18:2t10c12, 1:1) or as 18:2c9c12. During the last 2 d of AP and the last 4 d of EP feed intake and milk yield were recorded daily and milk samples were collected at each milking. Blood samples were collected from the jugular vein on d 11 of AP and d 15 and 18 of EP. The 18:2 intake increased in all treatments from AP to EP. Regardless of the amount of supplemented CLA, the milk fat percentage decreased by 2.35 and 2.10%-units in treatment C and SC, respectively, whereas in the treatment S the decrease was with 0.99%-unit less pronounced. Thus, C and SC cows excreted daily a lower amount of milk fat than S cows. The concentration of trans 18:1 in the plasma and the milk increased from AP to EP and increased with increasing dietary CLA supply. While the concentration of 18:2c9t11 and 18:2t10c12 in the plasma and that of 18:2t10c12 in the milk paralleled dietary supply, the level of 18:2c9t11 in the milk was similar in C and CS but still lower in S. Although the dietary concentration of CLA was highest in treatment C, the partial replacement of CLA by sunflower seeds had a similar inhibitory effect on milk fat synthesis. Comparable 18:2c9t11 levels in the milk in both CLA treatments implies that this isomer is subjected to greater biohydrogenation with increasing supply than 18:2t10c12. The fact that unprotected 18:2t10c12 escaped biohydrogenation in sufficient amounts to affect milk fat synthesis reveals opportunities to develop feeding strategies where reduced milk fat production is desirable or required by the metabolic state of the cow.

• Korean Journal of Food Science and Technology
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• v.46 no.2
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• pp.121-126
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• 2014

Recently, infant formula products made of caprine milk have gained popularity, mainly because the nutritional composition of caprine milk is similar to that of human milk. In addition, caprine milk is considered to be better than bovine milk in terms of nutrient composition and easier digestion. Compared to bovine milk, caprine milk contains more ${\beta}$-casein, but less ${\alpha}$S1-casein. While the lactose concentration of both bovine and caprine milk is almost the same, a content of total oligosaccharides in caprine milk was approximately five to eight times higher than that in bovine milk. However, as the dairy goat industry in Korea is in a nascent stage of milk production and further processing, many nutritional advantages of caprine milk over bovine milk are not fully conveyed to general consumers. It is recommended that scientific research regarding the nutritional benefits of caprine milk needs to be conducted urgently, owing to the increasing domestic sales of infant formula products made of caprine milk.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.6
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• pp.905-908
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• 2006

The purpose of this research was to study the effects of kefir whey (kefir whey, peptides, lactic acid) on skin care properties including skin lightening effect and acne treatment. The final aim was to develop a new cosmetic product and enhance the value of dairy products. The results of skin lightening tests showed that all three kefir whey components (kefir whey, peptides and lactic acid) had inhibitory ability against melanin synthesis. Furthermore, copper chelating analysis demonstrated that both kefir whey and kefir whey peptides could chelate the copper in tyrosinase, which might explain the mechanism of inhibition. The ability for acne treatment indicated that lactic acid level higher than 60 mg/ml could inhibit the growth of Propionibacterium acne, whereas no inhibition was found with other components.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.1247-1247
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• 2001

Constituents of animal biofluids such as milk, blood and urine contain information specifically related to metabolic and health status of the ruminant animals. Some changes in composition of biofluids can be attributed to disease response of the animals. Mastitis is a major problem for the global dairy industry and causes substantial economic losses from decreasing milk production and reducing milk quality. The purpose of this study was to investigate potential of NIRS combined with multivariate analysis for cow's mastitis diagnosis based on NIR spectra of milk, blood and urine. A total of 112 bulk milk, urine and blood samples from 4 Holstein cows were analyzed. The milk samples were collected from morning milking. The urine samples were collected before morning milking and stored at -35$^{\circ}C$ until spectral analysis. The blood samples were collected before morning milking using a catheter inserted into the carotid vein. Heparin was added to blood samples to prevent coagulation. All milk samples were analyzed for somatic cell count (SCC). The SCC content in milk was used as indicator of mastitis and as quantitative parameter for respective urine and blood samples collected at same time. NIR spectra of blood and milk samples were obtained by InfraAlyzer 500 spectrophotometer, using a transflectance mode. NIR spectra of urine samples were obtained by NIR System 6500 spectrophotometer, using 1 mm sample thickness. All samples were divided into calibration set and test set. Class variable was assigned for each sample as follow: healthy (class 1) and mastitic (class 2), based on milk SCC content. SIMCA was implemented to create models of the respective classes based on NIR spectra of milk, blood or urine. For the calibration set of samples, SIMCA models (model for samples from healthy cows and model for samples from mastitic cows), correctly classified from 97.33 to 98.67% of milk samples, from 97.33 to 98.61% of urine samples and from 96.00 to 94.67% of blood samples. From samples in the test set, the percent of correctly classified samples varied from 70.27 to 89.19, depending mainly on spectral data pretreatment. The best results for all data sets were obtained when first derivative spectral data pretreatment was used. The incorrect classified samples were 5 from milk samples,5 and 4 from urine and blood samples, respectively. The analysis of changes in the loading of first PC factor for group of samples from healthy cows and group of samples from mastitic cows showed, that separation between classes was indirect and based on influence of mastitis on the milk, blood and urine components. Results from the present investigation showed that the changes that occur when a cow gets mastitis influence her milk, urine and blood spectra in a specific way. SIMCA allowed extraction of available spectral information from the milk, urine and blood spectra connected with mastitis. The obtained results could be used for development of a new method for mastitis detection.

• Asian-Australasian Journal of Animal Sciences
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• v.7 no.1
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• pp.49-55
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• 1994

This study was conducted to compare the of rearing native and cross-bred dairy cows and returns received from them. For this purpose, 144 cows of 132 household from 24 villages were randomly selected. of them, 96 were native cows and 48 were cross-bred cows. The study revealed that among the structure of cost components, labour charge occupied the major share in the total cost of milk production per litre. The total cost of rearing native and cross-bred cows was Tk. 14,155 and Tk. 19,854 per annum, respectively. The average net cost of milk production per litre was Tk. 14.12 for native cows and Tk. 0.52 and for cross-bred cows were Taka 3.40. The benefit-cost ratio of milk per litre was higher (1:1.33) in cross-bred cows than native ones (1:1.04). The study further showed that in comparing with bulk line cost, the price of milk per litre received by the farmers was higher in cross-bred cows than native cows. Therefore, the study recommends dairying with cross-bred cows as encouraging and viable commercial enterprise in Banfladesh.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.5
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• pp.575-579
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• 2000

First lactation records of 683 Murrah buffaloes maintained at NDRI, Karnal which were progeny of 84 sires used for comparing the heritability estimates of age at first calving, first lactation milk yield and first service period under single and multiple trait models using restricted maximum likelihood (REML) method of estimation under an individual animal model. The results indicated that the heritability estimates may vary under single and multiple trait models depending upon the magnitude of genetic and environmental correlation among the traits being considered. Therefore, a single or multiple trait model is recommended for estimation of variance components depending upon the goal of breeding programme. However, there may not be any advantage of considering a trait with zero or near zero heritability and having no or very low genetic correlation with other traits in the model. Lower heritability estimates of part lactation yield (120-day milk yield) implied that there may not be any advantage of considering this trait in place of actual 305-day milk yield, whereas, comparable heritability estimates of predicted 305-day milk yield suggested that it could be used for sire evaluation to reduce the cost of milk recording under field conditions.

• Journal of Nutrition and Health
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• v.27 no.3
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• pp.263-271
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• 1994

The concentrations of the immunological substances in breast milk and nutritional status were studied in healthy Korean women of middle socioeconomic class. The subjects were recruited at random from obstetric clinics in Seoul. The nutrients intake, prepregnancy BMI, maternal weight gain during pregnancy were studied. The concentrations of lactoferrin(LF), lysozyme(LZ), sIgA, IgG and C3 in colostrum, in transitional milk, and in mature milk, were measured. To elucidate the effect of nutritional status on immunological substances, each components was compared on the basis of either BMI, weight gain, or protein intake. The highest concentrations of the substances were found in colostrum and decreased as lactation progressed. The decline was more prominent in IgG, C3 and sIgA, and less significant in LZ and LF. The colostrum of standard weight gain group showed higher concentrations compared to lower weight gain group. This difference became smaller as the lactation progresses. BMI and nutrient intake status had less significant effect. Lower sIgA was found in lower BMI, in lower weight gain, and lower protein intake groups compared to standard groups, which indicates sIgA is the most affected substance among the measured by nutritional status.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 1999.12a
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• pp.340-345
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• 1999