• 수산경영론집
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• 제49권2호
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• pp.49-65
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• 2018

The aim of this study is to develop suitability site indicator of urban-rural return to fishing and to analysis priority of the indicators. The study is based on literature review, expert survey and AHP(Analytic Hierarchy Process) methods. This study found 5 factors(natural environment, acceptance environment, social overhead capital, economy, fishing environment) and 21 detail variables. as a result of AHP, fisheries resources, acquisition convenience of fishing license, income(except fishing), attitude of local resident, one-person household income, high income fishing were showed high priority. This result indicate that economic foundation is most important factor for suitable site of the returning.

• 과학기술학연구
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• 제18권3호
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• pp.45-101
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• 2018

This paper discusses an important dimension of the globalization of science by investigating the lived experiences of nineteen expatriate scientists in six institutions in South Korea. Although much has been written on the globalization and science, few works have dealt with scientific experts? migration across national borders in contemporary contexts. In this regard, the lives of foreign science professors in Korea offer an interesting case, as they illustrate how the ideas of global science both clash with and appropriate Korea?s local practices and discourses. I argue that the globality of science is a main factor that fosters the continued Korean stay of many foreign scientists, who manage and appropriate what is entailed in this globality, namely, Korea?s perceived status as a peripheral country in the dynamics between "center" and "periphery." This includes several strategies and unintended situations differing according to each foreign scientist?s ethnic origins, professional experiences, and institutional circumstances, which lead them to make sense of their continued stay in Korea.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.182.2-182.2
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• 2003

The abnormal proliferation and migration of vascular smooth muscle cell (SMC) play an important role in the pathology of coronary artery atherosclerosis and restenosis. Platelet-derived growth factor (PDGF) is one of the most potent promoters of the proliferation and migration of the SMC. The heterocyclic quinones represent an important class of biologically active molecules. However, the inhibitory activity of quinone classes on the proliferation of the SMC has not been reported. (omitted)

• Journal of Ginseng Research
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• 제20권1호
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• pp.15-22
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• 1996

In order to investigate the Immunomodulatory effects of ginseng, we have studied the effects of ginseng saponin on the proliferation and cytosine gene expression of human pheripheral blood mononuclear cell (PBMC). In the PBMC proliferation assay, total saponin exhibited proliferation inhibition on the PBMC or phytohemagglutinin(PHA)-stimulated PBMC in a dose-dependent fashion. Immunomodulatory effects of ginseng were further investigated using the cytokine gene expression as the indicators. In the reverse transcription-polymerase chain reaction (RT-PCR) test, interleukin (IL)-1, IL-2, IL-3, IL-4, IL-6, IL-13, granulocyte macrophage-colony stimulating factor, tumor necrosis factor (TNF), migration inhibitory factor and transforming growth factor genes were expressed in the PHA-stimulated PBMC 48 hrs after cell culture. Among expressed cytokines, total saponin could increase the expression of IL-1 and TNF of PBMC without stimulation of PHA. All of ginsenosides, $Rb_1$, $Rb_2$, $Rg_1$, Rc, Re, incresed TNF gene expression. Especially, Rb2 (20 g/ml) showed most prominent effect on TNF gene expression and it also slightly increased IL-1 gene expression of PBMC.

• Molecules and Cells
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• 제20권3호
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• pp.429-434
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• 2005

Lysophosphatidylcholine (lysoPC) induces vascular smooth muscle cell (VSMC) proliferation and migration, which has been proposed to initiate the intimal thickening in coronary atherosclerotic lesions. Berberine is an alkaloid in Berberis aquifolium and many other plants. Recently, it has been shown to have beneficial effects on the cardiovascular system, such as anti-hyperglycemic and cholesterol-lowering activity. In this study, we investigated its effects on lysoPC-induced VSMC proliferation and migration. Berberine inhibited lysoPC-induced DNA synthesis and cell proliferation in VSMCs, as well as migration of the lysoPC-stimulated VSMCs. It also inhibited the activation of extracellular signal-regulated kinases (ERKs) and reduced transcription factor AP-1 activity and the lysoPC-induced increases in intracellular reactive oxygen species (ROS). These results indicate that the inhibitory effects of berberine on lysoPC-stimulated VSMC proliferation and migration are attributable to inhibition of ROS generation and hence of activation of the ERK1/2 pathway. This suggests that berberine has potential in the prevention of atherosclerosis and restenosis.

• Molecules and Cells
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• 제40권5호
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• pp.363-370
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• 2017

Extrahepatic cholangiocarcinoma (ECC), a malignant tumor of biliary origin, has a poor prognosis with limited treatment options. The KRAS oncogene is the most commonly mutated gene in ECC and one of the factors that predicts a poor prognosis and low survival rate. L1 cell adhesion molecule (L1CAM) is expressed in ECC cells and acts as an independent poor prognostic factor in predicting patient survival. In this study we investigate the functional significance of L1CAM in ECC cells with activating KRAS mutation. We selected an ECC cell line, EGI-1, with activating KRAS mutation, and then confirmed its expression of L1CAM by RT-PCR, western blot analysis, and flow cytometry. The suppression of L1CAM expression (using a specific lentivirus-delivered shRNA) significantly decreased the migratory and invasive properties of EGI-1 cells, without altering their proliferation or survival. Analyses of signaling effectors in L1CAM-depleted and control EGI-1 cells indicated that L1CAM suppression decreased the levels of both phosphorylated MKK4 and total MKK4, together with c-Jun N-terminal kinase (JNK) phosphorylation. Further, exposure to a JNK inhibitor (SP600125) decreased migration and invasion of EGI-1 cells. These results suggest that L1CAM promotes cellular migration and invasion via the induction of MKK4 expression, leading to JNK activation. Our study is the first to demonstrate a functional role for L1CAM in ECC carrying the activating KRAS mutation. Given that KRAS is the most commonly mutated oncogene in ECC, L1CAM may serve as an attractive therapeutic target for ECC cells with activating KRAS mutation.

• 지역연구
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• 제35권2호
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• pp.49-57
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• 2019

생애주기단계에서 청년층은 그 사회적위치가 빠르게 변화하는 연령대로, 본 연구는 청년층의 인구이동에 주목하고 주택시장요인을 포함한 지역특성요인과 청년층 인구이동의 관계를 서울지역을 중심으로 실증 분석하였다. 분석결과, 청년층의 이동은 거리와 일자리는 중요한 이동의 원인이 되고 있다. 이외에 주택시장에서의 전세가격이나 주택공급이 청년층 이동에 유의한 영향 요인으로 작용하는 것으로 분석되었다. 또한 서울시내 개별 지역과 이동의 기원지간의 전세가격과 같은 가격의 차이가 커질수록, 주택공급 차이가 커질수록 서울시 청년층의 순전출이 지속될 수 있음을 유추할 수 있다. 분석 결과 일자리의 경우, 청년층 유입을 증가시키는 데 기여하고 있어 고용중심지 주변에서의 청년층 주거수요는 높아질 것으로 예상된다. 따라서 현재 서울시나 정부에서 추진하고 있는 일자리가 많은 지역주변이나 역세권 주변에서의 청년층을 대상으로 한 공공주택을 비롯한 민간의 주택공급이 원활하게 이루어질 필요가 있으며, 저렴한 가격으로 거주할 수 있는 공적 주택이나 민간임대 주택 공급이 지속된다면, 서울시 청년층 전출을 줄이는 데 기여할 수 있을 것으로 기대된다.

• 한국수자원학회논문집
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• 제56권12호
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• pp.895-903
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• 2023

본 연구에서는 실내실험을 수행하여 홍수터 식생에 의한 하안의 안정성, 만곡부의 곡률 변화, 사행의 발달과 이동을 정량적으로 분석하였다. 홍수터에 식생이 있는 하도는 시간이 증가하면서 사행의 하폭이 일정하게 유지하지만, 선택적인 하안침식에 의해 사행이 발달하고 하류로 이동하였다. 이러한 과정에서 하안침식과 사주의 변화가 크지 않으며, 저수로가 일정하게 유지되면서 유사유출량은 감소하고 변동성이 거의 없이 일정하게 유지되고 있다. 식생의 밀도가 증가함에 따라, 하안침식률이 감소하였다. 식생의 밀도가 증가함에 따라 하안침식률이 감소하였고, 사행의 발달에 영향을 주었다. 이는 홍수터 식생이 하안의 안정성을 증가시키고, 하도의 평면변화에 영향을 미치는 주요 인자 중의 하나임을 의미한다. 하안침식률과 무차원 곡률반경은 홍수터에 식생이 없는 조건에서 가장 크고, 식생이 있는 조건에서는 작다. 또한 상대 측방이동률과 무차원 곡률반경도 하안침식률과의 관계가 유사하는 특성을 보였다. 따라서 홍수터 식생은 하도의 안정성을 증가시켜서 하안침식과 사행의 발달뿐만 아니라, 사행의 곡률 변화와 이동에 영향을 준다.

• 생명과학회지
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• 제33권2호
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• pp.129-137
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• 2023

활성산소는 세포의 다양한 생리활성에 중요한 역할을 수행한다. 본 연구에서는 리소포스파티드산에 의해 유도되는 SKOV-3 세포의 이동을 조절하는 신호전달 기전 연구를 수행하였다. IGF-1 및 LPA는 처리시간 그리고 용량 의존적으로 SKOV-3 세포의 이동을 촉진시켰으며, 리소포스파티드산은 이에 따라 Akt의 인산화도 촉진하였다. 리소포스파티드산에 의한 세포이동은 리소포스파티드산 수용체 억제제에 의해 길항되었으나 IGF-1에 의한 세포이동에는 영향이 없었다. PI3K 및 ROCK의 억제는 리소포스파티드산에 의한 세포의 이동을 길항하였으나 MAPK 억제제에 의해서는 길항되지 않았다. 리소포스파티드산에 의해 형성되는 활성산소는 PI3K 및 ROCK의 억제제에 의해 길항되었으며 활성산소를 킬레이트화하면 리소포스파티드산에 의한 세포의 이동이 억제되었다. 또한 리간드에 의해 활성산소를 형성하는 NOX를 억제하면 리소포스파티드산에 의한 세포의 이동도 억제 되는 것이 관찰되었다. Rictor 및 Akt1의 발현을 억제하면 활성산소 및 세포의 이동이 저해되었으나 Raptor 및 Akt2의 발현조절은 모두 영향이 없는 것으로 관찰되었다. 마지막으로 우성활성화형태인 Akt1의 과발현은 리소포스파티드산의 자극이 없어도 SKOV-3 세포의 이동을 촉진하는 것으로 관찰되었다. 이러한 결과들을 바탕으로 리소포스파티드산은 mTORC2/Akt1/NOX 신호전달 기전을 통해 활성산소를 형성하고 SKOV-3 난소암세포의 이동을 촉진한다는 것을 제안한다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권4호
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• pp.1375-1383
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• 2015

Background: Objective Myocardin-related transcription factor (MRTF)-A is a Rho signaling-responsive co-activator of serum response factor (SRF). The purpose of this study is to investigate the role of MRTF-A and AQP1 (aquaporin 1) in pathological vascular remodeling. Materials and Methods: MRTF-A, AQP1 and neointima expression was detected both in the wire injured femoral arteries of wild-type mice and the atherosclerotic aortic tissues of $ApoE^{-/-}$ mice. Expression of ICAM-1, matrix metallopeptidase 9 (MMP-9) and integrin ${\beta}1$ were also assayed. The intercourse relationship between the molecules were investigated by interfering RNA and inhibitor assay. Results: MRTF-A and AQP1 expression were significantly higher in the wire injured femoral arteries of wild-type mice and in the atherosclerotic aortic tissues of $ApoE^{-/-}$ mice than in healthy control tissues. Both in wire-injured femoral arteries in MRTF-A knockout ($Mkl1^{-/-}$) mice and atherosclerotic lesions in $Mkl1^{-/-}$; $ApoE^{-/-}$ mice, neointima formation were significantly attenuated and the expression of AQP1 were significantly decreased. Expression of ICAM-1, matrix metallopeptidase 9 (MMP-9) and integrin ${\beta}1$, three SRF targets and key regulators of cell migration, and AQP1 in injured arteries was significantly weaker in $Mkl1^{-/-}$ mice than in wild-type mice. In cultured vascular smooth muscle cells (VSMCs), knocking down MRTF-A reduced expression of these genes and significantly impaired cell migration. Underlying the increased MRTF-A expression in dedifferentiated VSMCs were the down-regulation of microRNA-300. Moreover, the MRTF-A inhibitor CCG1423 significantly reduced neointima formation following wire injury in mice. Conclusions: MRTF-A could be a novel therapeutic target for the treatment of vascular diseases.