• BMB Reports
• /
• 제30권6호
• /
• pp.448-452
• /
• 1997

Polyclonal antibody of the boar sperminogen was used to characterize the boar sperm proteinase sperminogen. Boar sperminogen was purified from the acid extracts of the washed epididymal spermatozoa by gel filtration through a Sephadex G-100 column. followed by preparative SDS-PAGE. The sperminogen band was sliced out and was eluted from the gel matrix. The purified sperminogen was used to produce the polyclonal antibody of the boar sperminogen. When characterized on a Western blot, the final preparation of sperminogen appeared as a homogenous protein with a molecular weight of 32 kDa. The relative migration of sperminogen was distinctly different from the major components of the proacrosin-acrosin system as well as all the observable proacrosin activation by-products detected on the Western blot. The sperminogen antibody, however. cross-reacted with the proacrosin-acrosin system.

• 한국동물학회지
• /
• 제29권1호
• /
• pp.70-74
• /
• 1986

쥐 골격근의 크루드 미토콘드리아에 있는 시토크롬 옥시다제의 활성을 비교 하였다. 붉은색의 빠른 트윗치 근육은 가장 높은 효소 활성을 나타냈고, 흰색의 빠른 트읫치 근육은 가장 낮았으며, 붉은 색이며 느린 트윗치 근육은 그 중간이었다. 위 세가지 타입의 근육에서 힘 염색한 결과 시토크롬 옥시다제의 전기영동상의 이동성이 다르게 나타났다. 이동성의 순서는 타입 I > 타입 $II_A$ > 타입 $II_B$이었다. 면역학적 전기영동의 결과는 위의 결과를 뒷받침 하였다.

• Fibers and Polymers
• /
• 제6권4호
• /
• pp.277-283
• /
• 2005

In order to prepare high molecular weight poly(methyl methacrylate) (PMMA)/silver nanocomposite microspheres, methyl methacrylate was suspension-polymerized in the presence of silver nanoparticles at low temperature with 2,2'-azobis(2,4-dimethylvaleronitrile) as an initiator. The rate of conversion was increased by increasing the initiator concentration. When silver nanoparticles were added, the rate of polymerization decreased slightly. High monomer conversion (about $85\%$) was obtained in spite of low polymerization temperature of $30^{\circ}C$. Under controlled conditions, PMMA/silver microspheres with various number-average degrees of polymerization (6,000-37,000) were prepared. Morphology studies revealed that except for normal suspension microspheres with a smooth surface, a golf ball-like appearance of the microspheres was observed, due to the migration and aggregation of the hydrophilic silver nanoparticles at the sublayer beneath the microsphere's surface.

• 한국지역지리학회지
• /
• 제11권4호
• /
• pp.523-535
• /
• 2005

이 연구는 학습자들의 일상적인 생활 속에서 얻는 지리적 경험에 기초한 생활중심 교수학습의 모형 설계와 적용 가능성을 살펴보려고 한다. 생활중심 교수학습 모형의 효과를 실증적으로 검증하기 위하여 실험집단과 비교집단을 비교하여 수행하였다. 교수학습의 실제사례로 고등학교 2학년의 '인구이동과 인구변화'의 단원을 선정하였다. 실험 수업대상으로는 강원도 춘천시 C고등학교 2학년을 대상으로 하며 생활중심 교수학습 모형을 적용한 실험집단 2개 반과 적용하지 않은 비교집단 2개 반 모두 4개반의 122명이 선정되었다. 연구 결과는 다음과 같다. 첫째, 교사가 교수할 개념들을 체계화된 지식 구조로 교수하지 않을 경우 이에 대한 학습자의 인지구조 재구성은 보다 활발하게 이루어 질 수 없기 때문에, 고등학교 한국지리의 '인구이동과 인구변화' 단원의 지리개념은 상위개념인 이동에서, 기본개념인 이주, 하위개념인 이주 요인으로 위계화하고 학생들이 경험할 수 있는 일상적 지리경험을 알아보았다. 둘째, 지리과 생활 중심 교수학습 내용의 실제 사례로 학생 자신의 가족 이주사 및 가족수의 변화를 조사하는 과제학습지를 기초적 자료로 활용하였다. 셋째, 이 연구에서 개발한 수업모형은 암기식 수업보다 학업성취도 측면에서 효과가 더 높게 나타났다. 수업반응도 검사를 분석한 결과, 정의적인 측면에서는 수업의 흥미도, 만족도 수업내용의 활용성에서는 긍정적인 효과가 나타났다. 특히 이러한 수업모형에 의한 학습이 학생들의 흥미를 유발시킬 수 있다는 것을 알 수 있다. 그러나 지리적 탐구력 신장에는 도움이 되지 않았다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제19권4호
• /
• pp.601-604
• /
• 2006

The objective of this study was to investigate technical methods for extraction of mucopolysachharide-protein containing chondroitin sulfate from keel cartilage of chickens. The chemical composition of chicken keel cartilage was determined. For the preparation of mucopolysaccharide-protein from lyophilized chicken keel cartilage, hot water extraction and alcalase hydrolysis methods were examined. Results showed that the optimum condition of hot water extraction was incubation for 120 min with a yield of 40.09% and chondroitin sulfate content of 28.46%. For alcalase hydrolysis, the most effective condition was 2% alcalase in 10 volumes of distilled water for 120 min. The yield of hydrolysate was 75.87%, and chondroitin sulfate content was 26.61%. For further separation of chondroitin sulfate from the alcalase hydrolysate, which has a higher yield than that of hot water, 60% ethanol precipitation was performed. The yield of the ethanol precipitate was 21.41% and its chondroitin sulfate content was 46.31%. The hot water extract, alcalase hydrolysate and ethanol precipitate showed similar electrophoretic migration with standard chondroitin sulfate (chondroitin sulfate A), using cellulose acetate membrane electrophoresis. These results indicated that a significant amount of mucopolysaccharide-protein containing chondroitin sulfate could be acquired form chicken keel cartilage. Therefore, keel cartilage in chicken may provide an inexpensive source of chondroitin sulfate for commercial purposes.

• 한국추진공학회:학술대회논문집
• /
• 한국추진공학회 2005년도 제25회 추계학술대회논문집
• /
• pp.413-416
• /
• 2005

고분자경화제를 사용한 라이너와 HTPB 추진제의 접착력 및 접착공정을 개선연구를 수행하였다. HTPB와 TDI를 반응시켜 제조된 고분자경화제를 라이너에 사용하면, 접착계면에서 경화제의 이동현상이 줄어든다. 따라서 연소관 준비공정에서 내열재 연마 및 물질이동방지제 도포공정의 생략이 가능하며 HTPB 추진제와 라이너의 접착력이 증가되었다. 또한 가속노화 시험결과 접착력의 저하현상이 관찰되지 않았다.

• 한국추진공학회지
• /
• 제10권2호
• /
• pp.110-114
• /
• 2006

고분자경화제를 사용한 라이너와 HTPB 추진제의 접착력 및 접착공정의 개선연구를 수행하였다. HTPB와 TDI를 반응시켜 제조된 고분자경화제를 라이너에 사용하면, 접착계면에서 경화제의 이동현상이 줄어든다 따라서 연소관 준비공정에서 내열재 연마 및 물질이동방지제 도포공정의 생략이 가능하며 HTPB 추진제와 라이너의 접착력이 증가되었다. 또한 가속노화 시험결과 접착력의 저하현상이 관찰되지 않았다.

• Clinical Endoscopy
• /
• 제57권4호
• /
• pp.424-433
• /
• 2024

Colonic stent placement is a commonly used bridging strategy for surgery in patients with obstructive colorectal cancer. The procedure involves the placement of a self-expandable metallic stent (SEMS) across the obstructive lesion to restore intestinal patency and alleviate the symptoms of obstruction. By allowing patients to receive surgery in a planned and staged manner with time for preoperative optimization and bowel preparation, stent placement may reduce the need for emergency surgery, which is associated with higher complication rates and poorer outcomes. This review focuses on the role of colon stenting as a bridge to surgery in the management of obstructive colorectal cancer. SEMS as a bridge to surgery for left-sided colon cancer has been demonstrated to be particularly useful; however, further research is needed for its application in cases of right-sided colon cancer. Colon stent placement also has limitations and potential complications including stent migration, re-obstruction, and perforation. However, the timing of curative surgery after SEMS placement remains inconclusive. Considering the literature to date, performing surgery at an interval of approximately 2 weeks is considered appropriate. Therefore, colonic stent placement may be an effective strategy as a bridge to surgery in patients with obstructive colorectal cancer.

• Journal of Gastric Cancer
• /
• 제13권4호
• /
• pp.214-225
• /
• 2013

Purpose: Peritoneal lavage cytology is part of the routine staging workup for patients with advanced gastric cancer. However, no quality assurance study has been conducted to show variations or biases in peritoneal lavage cytology results. The aim of this study was to demonstrate a test execution variation in peritoneal lavage cytology between investigating surgeons. Materials and Methods: A prospective cohort study was designed for determination of the positive rate of peritoneal lavage cytology using a liquid-based preparation method in patients with potentially curable advanced gastric cancer (cT2~4/N0~2/M0). One hundred thirty patients were enrolled and underwent laparotomy, peritoneal lavage cytology, and standard gastrectomy, which were performed by 3 investigating surgeons. Data were analyzed using the chi-square test and a logistic regression model. Results: The overall positive peritoneal cytology rate was 10.0%. Subgroup positive rates were 5.3% in pT1 cancer, 2.0% in pT2/3 cancer, 11.1% in pT4a cancer, and 71.4% in pT4b cancer. In univariate analysis, positive peritoneal cytology showed significant correlation with pT stage, lymphatic invasion, vascular invasion, ascites, and the investigating surgeon. We found the positive rate to be 2.1% for surgeon A, 10.2% for surgeon B, and 20.6% for surgeon C (P=0.024). Multivariate analysis identified pT stage, ascites, and the investigating surgeon to be significant risk factors for positive peritoneal cytology. Conclusions: The peritoneal lavage cytology results were significantly affected by the investigating surgeon, providing strong evidence of test execution variation that could be related to poor diagnostic accuracy and stage migration in patients with advanced gastric cancer.

• Nutritional Sciences
• /
• 제8권3호
• /
• pp.153-158
• /
• 2005

Numerous natural herbal compounds have been reported to inhibit adhesion and migration of leukocytes to the site of inflammation Licorice extracts, which have been widely used in traditional Chinese medicinal preparation, possess various pharmacological effects. Isoliquiritigenin, a biogenetic precursor of flavonoids with various pharmacological effects, is a natural pigment present in licorice. We attempted to explore whether licorice extracts and isoliquiritigenin mitigate monocyte adhesion to tumor necrosis factor-$\alpha$ (TNF-$\alpha$)-activated human umbilical vein endothelial cells (HUVEC). In addition, it was tested whether the inhibition of monocyte adhesion to the activated HUVEC accompanied a reduction in vascular cell adhesion molecule-l expression(VCAM-l). Dry-roasted licorice extracts in methylene chloride but not in ethanol markedly interfered with THP-l monocyte adhesion to INF-$\alpha$-activated endothelial cells. licochalcone A compound isolated from licorice extract in methylene chloride appeared to modestly inhibit the interaction of THP-l monocytes and activated endothelium. In addition, isoliquiritigenin abolished the monocyte adhesion with attenuating VCAM-l protein expression on HUVEC induced by INF-$\alpha$. These results demonstrated that non-polar components from dry-roasted licorice extracts containing licochalcone A as well as isoliquiritigenin were active in blocking monocyte adhesion to cytokine-activated endothelimn, which appeared to be mediated most likely through the inhibition of VCAM-l expression on HUVEC. Therefore, licorice may hamper initial inflammatory events on the vascular endothelium involving induction of endothelial cell adhesion molecules.