• Natural Product Sciences
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• 제8권2호
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• pp.44-47
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• 2002

The effects of Eurycoma longifolia Jack on masculine copulatory behaviour were studied in the middle aged male Sprague-Dawley rats, 9 months old and retired breeders after dosing them with 500 mg/kg twice daily for 10 days prior to test. The test lasted for 30 minutes after a 20 minute adaptation period, was carried out on the 11th day during the dark phase of the light-dark cycle (2000-0700 hours) and in subdued light, using a modified copulation cage but with the presence of a piece of mirror of appropriate size to facilitate observation. Results showed that the mean values of EL-1, EL-2 and EL-3 of the control middle aged male rats were 103.20 sec, 91.21 sec and 80.00 sec but were significantly (p<0.05) increased to 118.40-120.20 sec, 101.24-171.28 sec and 100.42-110.21 sec respectively in the methanol-chloroform, methanol-butanol-water and methanol-butanol treated middle aged male rats. However, further results also showed that PEI-1 and PEI-2 of the control middle aged male rats were 182.30 sec and 257.2 sec but were significantly (p<0.05) decreased to 100.42-121.31 sec and 40.21-132.31 sec respectively in the methanol-chloroform-butanol-water and methanol-butanol treated middle aged male rats. In conclusion, this study showed that although E. longifolia Jack continued to enhance the sexual activity of the middle aged male rats by extending the duration of coitus and decreasing the refractory period between the different series of copulation, but to a smaller degree as compared to sexually active, adult male rats (Ang and Sim, 1997).

• Food Science and Biotechnology
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• 제16권6호
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• pp.1023-1028
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• 2007

This study was designed to determine whether dietary glutinous barley (GB) affects lipid metabolism in middle-aged rats previously fed a high-fat diet. To induce obesity, 20 male 9-month-old Sprague Dawley rats were raised for 1 month on a diet containing 20%(w/w) lipid. The rats were allocated to 1 of 2 groups of 10 rats each and for the subsequent 2 months were fed an 8%(w/w) lipid diet containing well-milled rice (WMR) or GB powder. Rats fed the GB diet had significantly lower concentrations of plasma triglyceride, plasma total cholesterol, and liver cholesterol than rats fed the WMR diet. Fecal excretions of triglyceride and bile acids were significantly greater for the GB group than for the WMR group. In conclusion, dietary GB has positive effects on lipid metabolism: it decreases plasma cholesterol concentration by increasing fecal excretion of bile acids.

• The Korean Journal of Physiology and Pharmacology
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• 제10권5호
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• pp.283-287
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• 2006

Although estrogen is known to playa role in fatty acid metabolism, it remains unclear whether fatty acid oxidation in mature female rats differs from fatty acid oxidation in peri-pubertal young rats. In this study, we measured fatty acid metabolism in the skeletal muscles and livers of 5 and 50 weeks old male and female rats. The rate of palmitate oxidation in the liver and gastrocnemius red in the 50-week-old female rats were elevated as compared to the 5-week-old females, whereas there were no differences in the male rats. The rate of palmitate oxidation in the gastrocnemius red was correlated inversely with intra-abdominal fat mass in the 5-week-old male and female rats, whereas the palmitate oxidation rate was positively correlated with fat mass in the liver and gastrocnemius red in the 50-week-old rats. HOMA-IR and plasma insulin levels were positively correlated with intra-abdominal fat mass in the pooled 50-week-old male and female rats, but this correlation was not apparent in 5-week-old rats. In summary, the rate of fatty acid oxidation measured in the middle-aged adult female rats was significantly higher than those measured in the peri-pubertal young female rats. This difference may be attributed to the influence of ovarian hormones.

• Biomolecules & Therapeutics
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• 제6권4호
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• pp.337-344
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• 1998

The objective of the present study was to investigate the effect of senescence on the binding properties of muscarinic receptors in the neostriatum of young (3 months), middle-aged (18 months) and aged (33 months) male Fischer 344 x Brown Norway hybrid rats by employing direct binding of selective radiolabeled antagonists. Using the selective M, muscarinic receptor antagonist, $[^3H]$AF-DX384, as the ligand, no significant difference in the maximal receptor density (Bmax) was observed in the neostriatum among any age-groups. In contrast, with the selective M, receptor antagonist, $[^3H]$4-DAMP, a significant increase in the number of muscarinic receptors was observed in neostriatal membrane fractions prepared from the aged animals relative to that observed in the young rats. For each ligand there was no age-related change in its affinity (Kd) for the muscarinic receptors. These results indicate that the observed age-related changes in the muscarinic receptor density may not be necessarily decremuntal and depend upon the muscarinic receptor subtype examined.

• The Korean Journal of Physiology and Pharmacology
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• 제21권6호
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• pp.633-641
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• 2017

The aim of this study is to investigate the effects of intermittent ladder-climbing exercise training on mitochondrial biogenesis and ER stress of the cardiac muscle in high fat diet-induced obese middle-aged rats. We induced obesity over 6 weeks of period in 40 male Sprague-Dawley rats around 50 weeks old, and were randomly divided into four experimental groups: chow, HFD, exercise+HFD, and exercise+chow. The exercising groups underwent high-intensity intermittent training using a ladder-climbing and weight exercise 3 days/week for a total of 8 weeks. High-fat diet and concurrent exercise resulted in no significant reduction in body weight but caused a significant reduction in visceral fat weight (p<0.05). Expression of $PPAR{\delta}$ increased in the exercise groups and was significantly increased in the high-fat diet+exercise group (p<0.05). Among the ER stress-related proteins, the expression levels of p-PERK and CHOP, related to cardiac muscle damage, were significantly higher in the cardiac muscle of the high-fat diet group (p<0.05), and were significantly reduced by intermittent ladder-climbing exercise training (p<0.05). Specifically, this reduction was greater when the rats underwent exercise after switching back to the chow diet with a reduced caloric intake. Collectively, these results suggest that the combination of intermittent ladder-climbing exercise training and a reduced caloric intake can decrease the levels of ER stress-related proteins that contribute to cardiac muscle damage in obesity and aging. However, additional validation is required to understand the effects of these changes on mitochondrial biogenesis during exercise.

• 한국체육학회지인문사회과학편
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• 제55권6호
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• pp.691-700
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• 2016

본 연구의 목적은 장기간의 지구성 운동 또는 저항성 운동이 중년 흰쥐의 골격근 내 단백질 합성과 분해에 미치는 영향을 규명하는 것이다. 50 주령의 male Wistar rat 30 마리를 이용하여 3집단(좌업, 지구성 운동집단, 저항성 운동집단)으로 무선배정한 후 12 주간 처치를 실시하였다. 연구결과 12주간의 저항성운동은 중년 쥐의 족저근 내 Akt/mTOR 신호전달체계를 활성화시켰고, FoxO1/MuRF1 단백질 발현을 저해하였다. 지구성 운동은 mTOR 신호전달체계에는 영향을 미치지 않았으나 FoxO1/MuRF1 단백질 발현을 저해하였고, AMPK/PGC-1α 발현을 증가시켰다. 요약하면 12주간의 운동트레이닝은 중년 흰쥐의 골격근 내 단백질 동화/이화반응에 긍정적인 영향을 미쳤으며, 지구성 운동은 근단백질 이화반응의 저해를 통해서 노화근육의 단백질 균형에 영향을 미치는 것으로 나타났다. 이러한 결과를 통해서 근 손실에 따른 근 기능의 저하가 가시화되기 시작하는 중년을 대상으로 실시되는 운동처방 시 근 감소증 예방 및 치료를 위해 저항성 운동만이 아니라 중강도 이상의 지구성 운동도 유효할 것으로 생각된다.

• 대한수의학회지
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• 제35권3호
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• pp.505-513
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• 1995

Seventy wild rats were caught from Seoul city, Kyonggi, Kangwon, Honam, and yongnam provinces. All of them were in same species, Rattus norvegicus, except two R rattus from Kyonggi province. Seventy sera from wild rats were studied by immunofluorescent antibody assay for evidence of infection by spotted fever group rickettsia. The antidody prevalance was 37.14%(26/70) for spotted fever group rickettsia. The sero-prevalence rates for spotted fever group rickettsia antibody was the hightest in Kyonggi province with 55.56%(10/18), yongnam province with 50.00%(10/20), Kangwon province with 25.00%(2/8), Seoul city with 18.75%(3/16), and Honam province with 12.50%(1/8). The sero-positive rates difference between sexes were higher in female with 46.15%(12/26) than in male with 31.81%(14/44) for spotted fever group rickettsia. Twenty six of 68 Rattus norvegicus with antibody for spotted fever group rickettsia were in subadult with 50.00%(6/12), young adult with 38.89%(7/18), middle-aged adult with 35.29%(6/17), and old adult with 33.33%(7/21). No antibody was detected from R rattus.

• 대한임상검사과학회지
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• 제54권4호
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• pp.307-315
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• 2022

중년 이후 많은 환자들이 배뇨 후 요실금을 앓고 있지만 아직 적절한 치료가 이뤄지지 않고 있다. 몇 가지 병태생리학적 기전이 제안되었지만 우리가 주목한 기전은 구해면체근과 해면체 조직의 위축으로 인한 요도의 확장이었다. 본 연구의 목적은 해면체와 요도의 해부학적 변화를 관찰하여 배뇨 후 요실금의 기전을 규명하는 것으로 8주령의 SD 수컷 랫드 그룹과 24개월 이상 된 SD 수컷 랫드 그룹으로 나누었다. 전체 음경에 대한 구부요도 및 해면체 면적의 비율은 두 그룹 간 통계적으로 유의하였다(P<0.05). 평활근 함량과 배뇨 후 요실금의 기전(nNOS, α-SMA)과 관련된 매개변수는 두 그룹 간 유의한 차이가 있었으며(P<0.05), 본 연구의 결과는 배뇨 후 요실금 연구에 기여할 것으로 기대된다.