• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2004년도 International Meeting of the Microbiological Society of Korea
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• pp.140-140
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• 2004

• Parasites, Hosts and Diseases
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• 제51권6호
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• pp.695-701
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• 2013

Opisthorchis viverrini infection causes inflammation and liver injury leading to periductal fibrosis. Little is known about the pathological alterations in bile canaliculi in opisthorchiasis. This study aimed to investigate bile canalicular alterations in O. viverrini-infected hamsters and to examine the chemopreventive effects of curcumin on such changes. Hamsters were infected with O. viverrini and one group of animals was fed with 1% dietary curcumin supplement. Animals were examined during the acute infection phase, days 21 and 30 post-infection (PI) and chronic infection phase (day 90 PI). Scanning electron microscopy revealed that in the infected group fed with a normal diet, bile canaliculi became slightly tortuous by 30 day PI and more tortuous at day 90 PI. Transmission electron microscopy showed a reduction in microvilli density of canaliculi starting at day 30 PI, with a marked loss of microvilli at day 90 PI. These ultrastructral changes were slightly seen at day 21 PI, which was similar to that found in infected animals fed with 1% curcumin-supplemented diet. Notably, curcumin treatment prevented the reduction of microvilli density, reduced the dilation of bile canaliculi, and decreased the tortuosity of the bile canaliculi relative to non-infected animals on a normal diet at days 30 and 90 PI. These results suggest that curcumin reduces alteration of bile canaliculi and may be a promising agent to prevent the onset of bile duct abnormalities induced by O. viverrini infection.

• 대한수의학회지
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• 제39권3호
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• pp.425-434
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• 1999

The morphological studies on the cecal development in the 60-, 90-, and 120-day-old fetuses and the newborns of Korean native goats were investigated by scanning and transmission electron microscopy. The results were summarized as follows ; Scanning electron microscopic studies : 1. In the 60-day-old fetuses, fold-like shapes protrusion on the cecal mucosa surface appeared. In the 90-day-old fetuses, the cecal villi appeared to be columnar shapes. In the 120-day-old fetuses, the cecal villi showed various tongue-like or columnar shapes. In the newborns, only the rudimental trace of the villi and the intestinal glands were observed. Transmission electron microscopic studies : 2. In the 60-day-old fetuses, the cecal epithelia were simple columnar in some areas and stratified columnar in others, and the epithelial cells contained nuclei, nucleoli, ER, mitochondria, Golgi complexes, zonula occludens, desmosomes, digitiform intercellular junctions, and large masses of the glycogen granules. 3. In the 90-day-old fetuses, the cecal epithelia were simple columnar in some area and stratified columnar in other. The microvilli of the cecal epithelia became much larger and longer than those in the 60-day-old fetuses, and intercellular junctions were developed, and increased numbers of ER, mitochondria, Golgi complexes were observed and the goblet cells contained a lot of the secretory granules. 4. In the 120-day-old fetuses, the cecal epithelia were only simple columnar in all areas. Microvilli and cytoplasmic organelles were well developed and the irregular annular nuclei were observed. 5. In the newborns, the cecal epithelia were covered with extensive microvilli, and the goblet cells with secretory granules were protruded into the lumen. And some goblet cells secreted the secretory granules into the lumen.

• 한국수산과학회지
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• 제34권1호
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• pp.21-26
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• 2001

Alginate를 가열에 의해 저분자화하여 저분자화에 따른 소화생리 특성을 검토하기 위해, 랫드에 저분자 alginate인 HAG-10,HAG-50, HAG-100 및 alginate를 장기간 섭취시켰을 때, 체중, 각종 장기의 무게와 길이 및 췌장과 소장의 소화효소활성과 단백질, DNA 및 RNA 함량을 측정하였고 소장응모의 미세구조의 변화를 관찰하였다. 랫드의 체중은 $5\%$와 $10\%$ HAG-50 및 $10\%$ alginate에서 체중의 증가가 유의적으로 현저히 억제되었다. 간장의 무게는 유의적인 차이가 없었고, 위의 무게와 길이는 $10\%$ HAG-10, $1\%$, $5\%$ 및$10\%$ HAG-50, $1\%$와 $5\%$ HAG-100 그리고 $5\%$ alginate에서 증가하는 경향을 보였으며, 췌장의 무게와 길이는 모두 약간 증가하였다. 소장과 맹장의 무게와 길이는 $1\%$와 $5\%$ HAG-10을 제외하고 모두 증가하였으나 대장의 무게와 길이는 전반적으로 감소하였다. 췌장의 amylase 활성은 유의적인 차이는 보이지 않았고, lipase 활성은 HAG-50에서 약간 저하하였으며, protease 활성은 $1\%$ HAG-10만 제외하고 유의적으로 저하하였다. 췌장의 단백질 함량은 모두 증가하였으나, DNA와 RNA 함량은 유의 적 인 차이가 없었다. 소장의 단백질 함량은 $5\%$와 $10\%$ HAG-50에서 그리고DNA는 $5\%$ HAG-50에서 가장 높은 함량을 보였으며, RNA는 전반적으로 증가하였다 소장융모의 미세구조는 HAG-50에서 주름이 많고 표면적이 넓은 잎사귀 모양의 응모세포와 돌림주름 및 Boblet cell이 현저히 발달되어 있었다.

• 대한한의학회지
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• 제23권3호
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• pp.200-210
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• 2002

Objectives: Dojeckjiyu-tang has been used to treat Hwaseol & Jeokri. The object of this study is examination of the treatment effect of Dojeckjiyu-tang for ulcerative colitis of the mouse descending colon. Methods and Materials : Twenty-one rats were divided into 3 groups and treated as follows: the control group was untreated mice. The UCE group was ulcerative colitis elicited mice. The DJT group was Dojeckjiyu-tang treated mice after ulcerative colitis elicitation. The groups were examined with common morphology, paneth cells in intestinal crypt, absorptive cells and goblet cells in epithelium, cell division in mucose, COX-1 as mucosal protector, COX-2 (which appears to play an important role in inflammation), IL-2R-, ICMA-1-inducing cellular immuno-chainreaction, and the distribution of apoptotic cells. Results: 1. The morphology of colonic mucosa from UCE mice: the disappearance of epithelium and intestinal propria in hemorrhagic erosions were seen, but in the morphology of colonic mucosa from DJT-treated mice, the configuration of epithelium and intestinal propria were the same as normal. 2. The distribution of goblet cells and absorptive cells with microvilli in intestinal propria from UCE mice: a noticeable decrease of goblet cells and absorptive cells with microvilli were seen, but with the distribution of goblet cells and absorptive cells with microvilli in intestinal propria from DJT -treated mice, the configuration of goblet cells and absorptive cells with microvilli were the same as normal. 3. The immunohistochemical stain for BrdD in colonic mucosa and COX-1 in lamina propria from UCE mice: BrdU positive cells and COX-1 positive cells in the region of hemorrhagic erosion disappeared, but in the immunohistochemical stain for BrdU in colonic mucosa and COX-1 in lamina propria from DIT-treated mice, BrdU positive cells and COX-1 positive cells were seen. 4. The immunohistochemical stain for COX-2 in lamina propria, IL-2R-in lamina propria, intestinal propria and submucosa and ICMA-1 in intestinal propria and submucosa from DCE mice: a noticeable increase COX-2, IL-2R-, ICMA-1 positive cells were seen, but in the immunohistochemical stain for COX-2 in lamina propria, IL-2R-in lamina propria, intestinal propria and submucosa and ICMA-1 in intestinal propria and submucosa from DJT-treated mice, a numerical decrease of COX-2, IL-2R-, ICMA-1 positive cells was observed. 5. The distribution of apoptotic cells in epithelium and lamina propria from UCE mice: a noticeable increase of apoptotic cells in region of hemorrhagic erosion was seen, but in the distribution of apoptotic cells in epithelium and lamina propria from DJT-treated mice, a remarkable decrease of apoptotic cells was seen. Conclusions: According to the above results, Dojeckjiyu-tang has a moderate effect on ulcerative colitis in descending colon.

• Applied Microscopy
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• 제30권3호
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• pp.273-283
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• 2000

흰쥐에서 납 독성에 대한 녹차의 방어효과를 알아보기 위하여 150g 내외의 흰쥐를 대조군, 납 단독투여군, 납-녹차 투여군으로 나누어 4주, 8주간 lead acetate (500 ppm)를 1주에 2번씩 복강 투여하였고, 녹차침수액(3g of tea/100m1 of water)은 식수로 공급하였다. 생화학적 및 형태학적 실험을 통해 다음과 같은 결론을 얻었다. 1. ALP값은 대조군에 비해 실험군 모두 감소하였고, 납-녹차투여군은 납 단독투여군보다 감소가 완화되었다. BUN는 대조군에 비해 실험군 모두 증가하였고, 납 녹차투여군은 납 단독투여군보다 증가가 완화되었다. 2. 신장의 전자현미경적 결과 B-1군은 미세융모, 사립체가 변형되었다. 다수의 리소좀이 관찰되었다. 핵의 모양은 불규칙하게 나타났다. B-2군은 미세융모가 잘 발달되었다. 다수의 사립체와 리소좀이 관찰되었다. rER의 수조는 팽창되고 수가 감소하였고 핵은 정상적으로 관찰되었다. C-1군은 대부분의 미세융모가 탈락, 변성되었고, 다수의 리소좀을 관찰할 수 있었다. 사립체는 감소, 변형되었다. 핵은 함입되어 불규칙하였다. C-2군은 미세융모의 일부가 팽창되거나 변화되었고, 다른 세포소기관들은 B-2군과 비슷한 양상을 나타냈다.

• Applied Microscopy
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• 제37권4호
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• pp.219-230
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• 2007

마우스의 코 안으로 5% $ZnSO_4$용액을 점적하는 것이 코중격 후각상피에 미치는 영향과, 그 이후 후각수용세포가 재생되는 과정을 주사전자현미경과 투과전자현미경을 사용하여 형태학적으로 조사하였고, 그 결과는 다음과 같다. 1. 5% $ZnSO_4$용액을 점적한 후 6시간에서 24시간 사이에 코중격 후각상피층은 일부의 기저세포들을 제외하고는 완전하게 탈락되었다. 2. $ZnSO_4$처리 후 3일군에서는 코중격 후각상피세포들 중 부분적으로 남아 있던 기저세포들의 세포분열로 형성된 새로운 후각상피세포들로 후각상피층은 2층으로 나타났다. 또한 새롭게 형성된 후각상피세포들의 상부 세포막에는 미세응모가 돌출되었다. 3. 5% $ZnSO_4$용액을 점적하고 5일이 경과된 코중격 후각수용세포들에서는 다수의 중심소체와 기저소체가 관찰되었고, 상부 세포막에는 미세응모들 사이에 섬모들이 줄지어 나타났다. 또한 새롭게 형성된 코중격 후각수용세포들에서 처음으로 후각소포의 초기 형태가 나타났으며, 1주가 경과된 후각상피층에는 전형적인 형태의 후각소포를 관찰할 수 있었다. 4. 5% $ZnSO_4$용액 점적 후 2주가 경과된 경우, 성숙한 형태의 후각소포를 가지고 있는 후각수용세포가 관찰되었다. 이와 같은 결과는 5% $ZnSO_4$용액의 처리가 마우스 코중격의 후각상피층을 완전하게 박리시킬 수 있으므로 포유류 신경조직의 재생연구를 위해 유용한 실험적 모델임을 뒷받침한다, 또한 새롭게 재생되는 후각수용세포의 상부 세포막이 처음에는 미세응모로 표면적을 넓히고, 시간이 경과함에 따라 일렬로 배열된 섬모들로 대치되며, 그 후 섬모를 포함한 상부세포막이 부분적으로 돌출하고, 마지막으로 전형적인 후각소포로 발달한다.

• Applied Microscopy
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• 제24권3호
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• pp.34-45
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• 1994

This investigation has been carried out to examine the structure of digestive tract from Korean Leech, Erpobdella lineata, using light and electron microscope. The digestive tract is composed of mouth, pharynx, Oesophagus, six-chambered stomach, three-chambered intestine, rectum and anus. Stomach and intestine have not gastric or intestine ceca and consist of only straight tube. All digestive tracts from pharynx to rectum are covered with simple columnar epithelial cells. While the surfaces of endothelial cell of pharynx and rectum are covered with cuticular layer of about $0.3{\mu}m$ in thickness, stomach and intestine are covered with estimated $0.2-0.3{\mu}m$ and $0.5{\mu}m$ microvilli respectively. Circular folds were found only in first and second chambers of stomach, intestine and rectum, but not in pharynx and the other chambers (third to sixth) of stomach. The granules of $0.3-0.8{\mu}m$ and $0.5-1.0{\mu}m$ in diameter were observed in the cytoplasm of stomach endothelial cell. These granules were demonstrated to contain protein which showed a positive reaction to ninhydrin. It was also found that there are well-developed microvilli in the apical portion of intestine endothelial cell in which endocytosis occurs actively.

• Animal cells and systems
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• 제3권4호
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• pp.407-412
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• 1999

The influence of eating habits and food type on the ultrastructural characteristics of the digestive tracts was studied under the scanning and transmission electron microscopes in two crustacean decapods (Hemigrapsus penicillatus De Haan; mud crab, Pinnotheres cyclinus Shen; symbiotic crab). The relative ratio of the length of midgut versus hindgut was 1:1 in the mud crab, but 4:1 in the symbiotic crab. Observation through the scanning electron microscope revealed that the midguts of both species have densely-arranged longitudinal mucosal folds with a smooth surface. In the hindgut of the mud crab, mucosal folds were longitudinally oriented, clusters of two to five spines were observed on the cuticular surface, and the length of the spine in the distal hindgut was longer than that in the proximal portion. In the symbiotic crab, the mucosal folds were irregulary arranged, and numerous rudimentary spinal structures were noted on the cuticular surface. Through observation of a transmission electron microscope, the epithelial cells of the midgut in both species had numerous microvilli, but the length of the microvilli was slightly longer in the mud crab than in the symbiotic crab. The central layer of the basement membrane and the muscular layer of the midgut were more developed in the mud crab than in the symbiotic crab. The thickness of the cuticular layer over the hindgut surface in the mud crab was about 4 times than that of the symbiotic crab.

• Applied Microscopy
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• 제27권2호
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• pp.189-196
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• 1997

The ultrastrucures of Xenopus otic vesicle from normal embryo (st. 43) and induced otic vesicle from animal cap assay with Activin A were compared. Activin A was applied to the presumptive ectoderm at various concentrations for three days at $20^{\circ}C$. The results were almost identical to the reference studies, but it was found that the otic vesicle was induced at the concentration of 10 ng/ml in to)v rate. This otic vesicle may be secondarily induced by the neural tissue showed commonly at the concentration of 10 ng/ml. Otoliths were observed as three or four-axis crystaline bodies in the lumen of otic vesicle. In electron micrograph of the normal embryo, two kinds of microvilli were observed on the apical position of hair cells. One was small and common, the other was large-sized and sparsely distributed. In both cell of otic vesicle, mitochondria, golgi apparatus and multivesicular body were rich, so, they showed a lot of similarities in ultrastructure. However, the otolith was not found and microvilli were overexpressed in the otic vesicle induced by Activin A.