• 미생물학회지
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• 제7권1호
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• pp.29-40
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• 1969

Two kinds of organic materials, powders of Salicornia and Oryza sativa L. wre added as a source of organic matter to the suspensions of saline soils(soil : water = 1:3) to be 4 per cent of dried saline soil grams. And then, the samples were incubated at $28^{\circ}C$ to improve the decomposition of organic materials by soil microflora. Resutls of this experiment are summarized as followings : 1) The pH of soil suspension showed its highest value on the second or third week after the treatments, which were similar to those of the soil microflora. Results of this experiment are summarized as follwings : 1) The pH of soil suspension showed its highest value on the second ot third week after the treatments, which were similar to those of the soil microflora populations. 2) Salinity increased up to the second week and the highest value of it appeared in the samples which were treated with the powder of Salicornia. In general, the salinity of all samples decreased on the third week after the treatment. The fact was assumed the possibilities of desalination from saline soil by the microbial actions. 3) Soil microflora such as bacteria, actinomycetes and fungi, were determined its populations soil microflora is to act as decomposer in soil. Both of the bacteria and actinomycytes population showed in the third weak after the tratment. In general, 30-years old of saline soil contained microbial population much more than those of 5-year old of saline soil. Salicornia powder favored the increase of both the bacteria and fungal population, and Oryza sativa L. seemed to have been a great role in increase of actinomycetes. Especially, fungal population of the untreated soil suspension contained higher microbial populations more than those of the soils treated with both of the organic materials. 4) Sugar contents of soil suspesions decreased remarkably on the first week after the treatments. 5) The amount of nitrate had similar tendency to those of population changes of soil microflora. Total acidity decreased continuously and the amount of potassium showed its highest value on the third week treatment. However, the amount of phosphorus was determined to be insignificant.

• Asian-Australasian Journal of Animal Sciences
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• 제18권1호
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• pp.85-89
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• 2005

This study was conducted to examine the effects of green tea polyphenols (GTP) and fructo-oligosaccharides (FOS) supplement on performance, counts of caecal microflora and its metabolites production. In female broiler chickens fed on semi-purified diets from 28 to 42 d of age, dietary green tea polyphenols (GTP) and fructo-oligosaccharides (FOS) significantly reduced mortality (p<0.05). Dietary GTP significantly decreased the total count of caecal microflora, each colonic population count and caecal flora metabolites contents when compared to other groups (p<0.05). Dietary FOS did not influence the total count of caecal flora but it selectively increased Bifidobacteri and Eubacteria counts (p<0.05) and decreased the count of other microflora and concentrations of caecal phenols and indole (p<0.0.5). These results suggest that GTP and FOS in semi-purified diets can decrease mortality and change the caecal colonic flora population, but GTP shows antibiotic-like effects of non-selectively decreasing all colonic flora and then metabolites, and FOS acts selectively by increasing profitable microflora and decreasing production of caecal microflora metabolites besides volatile fatty acids.

• Journal of Plant Biology
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• 제11권4호
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• pp.9-20
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• 1968

In general there are few cases which were caused by lightening and no fire was observed to have been caused but by artificial or accidental fires during preliminary survey. And then the most scales of burning in Korea are like the fig. 2. Temperature 5 cm apart from flame at the burning site were known to range from 165$^{\circ}$to 20$0^{\circ}C$ in surface fire on Myozangdong, pine-quercus dominant community and from 120$^{\circ}$ to 145$^{\circ}C$ in surface fire of Wolwoonni, Mischanthus dominant area and from 120$^{\circ}$ to 14$0^{\circ}C$ in ground fire of Wangzinkun, Mischanthus dominant, respectively. Through the preliminary survey, fire indicater plants in Korea were confirmed as Mischanthus coreensis Hack and Carex alterifolia Franch. The plants of highest frequency appeared in the burnt sites were known to as Potentilla cryptotaeniae Max, Mischanthus coreensis Hack, Carex alterifolia Franch and Artemisia brachyphylla Kitamura. Lespedezas were proved as one of the fire tolerant and Pinus densiflora was found out as the lowest intolerant plant to fire. The acidity of burnt soil was decreased according to the considerable amount of ash left on the ground. The acidity of surface soil was more decreased than the below 10cm of soil. The chemical composition(NO3, P, K, Mg, Total-N and Organic-C) of burnt soil was more increased than those of the unburnt sites. And the population changes of soil microflora seemed to have a certain tendency. The population of soil microflora was increased a little according to climatic conditions. Also there was an initial decrease in the population of microflora followed immediately by a significant increase.

• Applied Biological Chemistry
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• 제49권3호
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• pp.175-179
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• 2006

동일비료를 장기연용한 토양에서 벼를 단작으로 재배하여 비료의 시용에 따른 벼 품종간 근권 세균 및 방선균의 밀도와 우점균을 조사하였다. 비료는 3요소(NPK)를 기본으로 하여 무질소(PK), 무인산(NK) 무칼리(NP) 및 NPK+퇴비를 처리하였다. 벼의 근권 미생물 밀도는 품종에 따라서는 큰 차이를 보이지 않았고 시용비료에 의해 약간의 차이를 보였는데 세균은 감소하고 방선균은 증가하는 경향을 나타내었다. 근권 미생물의 분포상에는 유기물의 영향이 뚜렷하여 방선균의 경우 NPK+퇴비구 처리구에서 가장 높은 균 밀도를 나타냈다. 배양가능한 호기성 세균에서는 Bacillus속의 B. megaterium, B. mycoides, B. licheniformis, B. subtilis 등이, 방선균에서는 Streptomyces속의 S. spororaveus, S. canus, S. tauricus, S. galbus 등과 Micromonospora속이 우점하여 분포하였다.

• 한국환경농학회지
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• 제31권4호
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• pp.368-374
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• 2012

BACKGROUND: The urgency of feeding the world's growing population while combating soil pollution, salinization and desertification requires suitable biotechnology not only to improve crop productivity but also to improve soil health through interactions of soil nutrient and soil microorganism. Interest in the utilization of microbial fertilizer has increased. A principle of nature farming is to produce abundant and healthy crops without using chemical fertilizer and pesticides, and without interrupting the natural ecosystem. Beneficial microorganisms may provide supplemental nutrients in the soil, promote crop growth, and enhance plant resistance against pathogenic microorganisms. We mixed beneficial microorganisms such as Bacillus sp. Han-5 with anti-fungal activities, Trichoderma harziaum, Trichoderma longibrachiatum with organic material degrading activity, Actinomycetes bovis with antibiotic production and Pseudomonas sp. with nitrogen fixation. This study was carried out to investigate the mixtures on the soil microflora and soil chemical properties and the effect on the growth of lettuce and cucumber under greenhouse conditions. METHODS AND RESULTS: The microbial mixtures were used with each of organic fertilizer, swine manure and organic+swine manure and compared in regard to changes in soil chemical properties, soil microflora properties and crop growth. At 50 days after the treatment of microorganism mixtures, the pH improved from 5.8 to 6.3, and the EC, $NO_3$-Na and K decreased by 52.4%, 60.5% and 29.3%, respectively. The available $P_2O_5$ and $SiO_2$ increased by 25.9% and 21.2%, respectively. Otherwise, the population density of fluorescent Pseudomonas sp. was accelerated and the growth of vegetables increased. Moreover, the population density of E. coli and Fusarium sp., decreased remarkably. The ratio of bacteria to fungi (B/F) and the ratio of Actinomycetes bovis to fungi (A/F) increased 2.3 (from 272.2 to 624.4) and 1.7 times (from 38.3 to 64), respectively. Furthermore, the growth and yield of cucumber and lettuce significantly increased by the treatment of microorganism mixtures. CONCLUSION(S): These results suggest that the treatment of microorganism mixtures improved the chemical properties and the microflora of soil and the crop growth. Therefore, it is concluded that the microorganism mixtures could be good alternative soil amendments to restore soil nutrients and soil microflora.

• 미생물학회지
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• 제8권2호
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• pp.53-64
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• 1970

In order to study ecology of microorganisms during Takju brewing, microflora changes were examined fromm the start to the sixth day of Takju fermentation in 24 hours intervals. Takju made from rice, flour and dried sweet potato in a liter volume open container at the laboratory and a sanple of Takju brewing factory were studied for their microflora and their changes during fermentationl together with a sample of Kokja. Results obtained were as follows ; 1. The followings were the identified microorganisms in Kokja. The molds ; Absidia spinosa, Aspergillus parasiticus. The yeasts ; Candida melinii, Candida Solani, Hansenula anomala. The bacteria ; Luctobacillus casei, Leuconostoc mesenteroides, Bacillus subtilis, Bacillus pumilus. 2. Torulopsis inconspicua, Lactobacillus casei, Leuconotoc mesenteroides, Bacillus subtilis, Bacillus pumilus were isolated from main mash of laboratory-made Takju samples. The yeast, Torupsis inconspicua which was not present in Kokja and, probably of a contaminant yeast, dominated the yeast flora of Takju mash of rice, flour and sweet potato of labotatory brewing. The laboratory brewing lost also always showed large population of lactic acid bacteria flora. 3. None of the wild yeasts which were present in Kokja appeared in Takju mashes. The Kokja appears to be of no use as the yeast source for Takju fermentation. Also the Kokja appears to be of not so effective amylolytic and proteolytic enzyme sources considering the microflora characteristics. Probably the major role of Kokja in Takju fermentation may be to contribute in taste formation. 4. Inoculation of Sacharomyces cerevisiae into the mash to the level of $10^7$ ml at the start of fermentation greatly changed the ecological aspects eliminating conditions of rather slow rising of natural contaminant yeast populaiton and fermentation which might give rise to prosperity of lactic acid and Bacillus bacteria that would be avoidable. 5. Examination of microflora of the large factory scale Takju fermentation showed the quite similar pattern of microflora and their changes to that of the cultured yeast-inoculated laboratory batch Takju fermentation. The cultured yeast dominated as the only predominant microflora, and the lactic acid bacteria flora were completely suppressed and aerobic bacteria, greatly. Probably this may be the regular microflora pattern of normal Takju fermentation. The role of lactic acid bacteria and aerobic bacteria in Takju fermentation may not be clear yet from this experiment alone.

• Nuclear Engineering and Technology
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• 제1권1호
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• pp.17-22
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• 1969

English sole(가재미종) 어편을 Polymylar bag에 밀봉하여 500 Krad의 감마선에 조사한 후 36일간의 0-2$^{\circ}C$ 저장기간중 조사한 것과 조사하지 않은 어편내에 축적되는 부패표시 물질과 총 균수를 측정하였다. 단백질 분해균을 직접 계수하기 위하여 Casein agar Plate 법을 만들어 저장기간중의 총균수비 대한 단백질분해균의 비율을 측정하는데 사용하였다. 500 Krad 선량 조사결과 어편의 총 균수는 10분의 1로 감축되었고 저장중 조사하지 않은 어편의 총균수 보다 훨씬 적어 1000분의 1선까지 내려갔다. 이에 따라 TVB와 TMA 축적도 억제되어 부패선을 넘지 못했으며 단백질 분해균 역시 총균수의 1% 이하선으로 감축되었으며 저장 기간중 이 선을 넘지 못했다. 반면에 조사하지 않은 어편엔 총 균수에 대한 이들의 비율은 저장기간중 점차 상승하여 22일째까지 총균수의 85.5%를 차지하였다. 방사선 조사로 인한 이 단백질 분해균의 선택적인 제거는 그 어편의 단백질 분해 부진 현상을 초래했다.

• Journal of Nutrition and Health
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• 제27권10호
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• pp.988-995
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• 1994

This study was performed to investigate the influence of various dietary fiber sources(rice bran, Chinese cabbage, radish, apple, laver, sea mustard) on the intestinal microflora in rats. Eight groups of rats were fed each experimental diets containing 5% of total dietary fiber for 4 weeks. Total viable counts and the numbers of Bifidobacterium, Lactobacillus, Clostridium, E. coli and Staphylococcus were determined by nonselective medium and various selective media. The number of Bifidobacterium(p<0.01) was higher in the apple and sea mustard groups than those in the other groups and the number of Lactobacillus(p<0.01) was lower in the radish group. A decrease in the intestinal population of clostridium was shown in the sea mustard group. The E. coli(p<0.01) and Staphylococcus(p<0.01) populations decreased in the apple group compared with other groups. These findings suggest that the apple fiber and sea mustard fiber are effective in improving intestinal flora of rats.

• Asian-Australasian Journal of Animal Sciences
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• 제22권8호
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• pp.1202-1208
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• 2009

Synbiotics is the term used for a mixture of probiotics (live microbial feed additives that beneficially affects the host animal) and prebiotics (non-digestible food ingredients that beneficially affect the organism). This study investigated the effect of probiotics from anaerobic microflora with prebiotics on growth performance, nutrient digestibility, noxious gas emission and fecal microbial population in weaning pigs. 150 pigs with an initial BW of 6.80${\pm}$0.32 kg (20 d of age) were randomly assigned to 5 dietary treatments as follows: i) US, basal diet+0.15% antibiotics (0.05% oxytetracycline 200 and 0.10% tiamulin 38 g), ii) BS, basal diet+0.2% synbiotics (probiotics from bacteria), iii) YS, basal diet+0.2% synbiotics (probiotics from yeast), iv) MS, basal diet+0.2% synbiotics (probiotics from mold), v) CS, basal diet+0.2% synbiotics (from compounds of bacteria, yeast and mold). The probiotics were contained in $10^{9}$ cfu/ml, $10^{5}$ cfu/ml and $10^{3}$ tfu/ml of bacteria, yeast and molds, respectively. The same prebiotics (mannan oligosaccharide, lactose, sodium acetate and ammonium citrate) was used for all the synbiotics. Pigs were housed individually for a 16-day experimental period. Growth performance showed no significant difference between antibiotic treatments and synbiotics-added treatments. The BS treatment showed higher (p<0.05) dry matter (DM) and nitrogen digestibility while ether extract and crude fiber digestibility were not affected by the dietary treatment. Also, the BS treatment decreased (p<0.05) fecal ammonia and amine gas emissions. Hydrogen sulfide concentration was also decreased (p<0.05) in BS, YS and MS treatments compared to other treatments. Moreover, all the synbioticsadded treatments increased fecal acetic acid concentration while the CS treatment had lower propionic acid concentration than the US treatment (p<0.05) gas emissions but decreased in fecal propionate gas emissions. Total fecal bacteria and Escherichia coli populations did not differ significantly among the treatments, while the Shigella counts were decreased (p<0.05) in synbiotics-included treatment. Fecal bacteria population was higher in the YS treatment than other treatments (p<0.05). The BS treatment had higher yeast concentration than YS, MS and CS treatments, while US treatment had higher mold concentrations than MS treatment (p<0.05). Therefore, the results of the present study suggest that synbiotics are as effective as antibiotics on growth performance, nutrient digestibility and fecal microflora composition in weaning pigs. Additionally, synbiotics from anaerobic microflora can decrease fecal noxious gas emission and synbiotics can substitute for antibiotics in weaning pigs.

• 한국유기농업학회지
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• 제12권1호
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• pp.81-92
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• 2004

The effect of feeding extracts from rhus trees on performance of broiler were performed to investigate development of natural antibiotic in process of broilers production. A total of 320 broiler chicks at 7 day of age were fed the commercial diet and water, water containing 0 ppm (control), 300 ppm (T1), 600 ppm (T2) and 1000 ppm (T3) of extracts from rims trees for 7 weeks. The body weight gain and feed conversion ratio were improvement by feeding extracts from rims trees at 7 to 21 days of age (P<0.05), but amount of feed intake and mortality were not different among the four group. When 21 to 35 days of age, body weight gain, amount of feed intake, feed conversion ratio and mortality were almost same results when 7 to 21days of age, but it was not significantly different. When 35 to 49 days of age, body weight gain in T1 and T3 group was significantly improvement than control group (P<0.05), also feed conversion ratio was improve by feeding extracts from rhus trees. There was a decrease in the microflora population of both E coli. and salmonella in the cecum contents and feaces in broilers by feeding extracts from rhus trees. Emission of ammonia and VFA gas from excreta were significantly decreased at 5 to 10 days of storaged period (P<0.05). The digestibility of dry matter, crude fat, and crude ash of feed were tend to increased in broiler feeding extracts from rims trees. Digestibility of crude protein of feed in broiler three treatmental groups was significantly improved (P<0.05) as compared with those in control group. These results indicated that the feeding extracts from rhus trees of 300 to 1000 ppm of broiler production were improvement in th6 body weight gain, feed conversion ratio, and mortality due to increasing nutrients digestibility from feed, decreasing of microflora population of both E coll. and salmonella in the cecum contents and feaces, decreasing emission of ammonia and VFA gas from excreta.