• Title, Summary, Keyword: Microencapsulation

Search Result 154, Processing Time 0.03 seconds

The Development of Microparticle Feed Using Microencapsulation (Microencapsulation을 이용한 미립자 사료개발)

  • 이은주;김성구
    • Journal of Life Science
    • /
    • v.6 no.2
    • /
    • pp.129-134
    • /
    • 1996
  • The development of fish feed is essential to aquaculture. Recently, yeast, dhlorella and plankton have been studied and development as the feed of the fry fishes, But, these biological feeds cause the nutritional unbalance to fry fishes, rotifer or artemia. Therefore, to solve these problems, microcapsules with micron sizes were prepared for enhancing the nutritional values of artemia and rotifer which are used as the feed of fry fishes. Microparticle oil capsules were prepared by the complex coacervation technique. The method to make the optimal size of microcapsule which the artemia and rotifer can be easily taken was wvaluated. The size of oil microcapsule in the range of 5-70$\mu$m was obtained by the agitation conditions during coacervation. Capsule size and size distribution were dependent on the agitation speed and agitation time, respectively.

  • PDF

Development of Susceptible Functional Fibers Using Microencapsulation of Susceptible Materials(III) ―Fragrant Functional Fibers― (감성물질의 마이크로캡슐화에 의한 감성기능 섬유의 개발(III) -방향 기능 섬유-)

  • Kim, Moon Sik;Park, Soo Min
    • Textile Coloration and Finishing
    • /
    • v.8 no.4
    • /
    • pp.11-18
    • /
    • 1996
  • Natural functional compound in the textile finishing for health and amenity using fragrant material have been applied by microencapsulation method. The microcapsules containing fragrant materials as functional compound were produced by in situ polymerization using urea-formaldehyde prepolymer. The average diameter of microcapsules is 2.75$\mu$ and particle size ranges over 0.5~10$\mu$. Fragment material is extracted approximatly proportioned from microcapsule at room temperature. The adsorption of microcapsule was improved by pretreatment of cationic agent. Fragrant materials in microcapsule was revealed to have long release time.

  • PDF

Antigenicity of Protein Entrapped in Poly(lactide-co-glycolide) Microspheres (폴리락티드-글리콜리드 마이크로스피어에 봉입된 단백질의 항원성 평가)

  • Song, Seh-Hyon;Cho, Seong-Wan;Shin, Taek-Hwan;Yoon, Mi-Kyoung;Choi, Young-Wook
    • Journal of Pharmaceutical Investigation
    • /
    • v.31 no.3
    • /
    • pp.191-196
    • /
    • 2001
  • Biodegradable polymeric microspheres were studied for their usefulness as carriers for the delivery of vaccine antigens. However, protein antigen could be denatured during microencapsulation processes due to the exposure to the organic phase and stress condition of cavitation and shear force. Therefore this study was carried out to re-evaluate the degree of protein denaturation during microencapsulation with poly(lactide-co-glycolide) (PLGA) copolymer. PLGA microspheres containing ovalbumin (OVA), prepared by W/O/W multiple emulsification method, were suspended in pH 7.4 PBS and incubated with shaking at $37.5^{\circ}C$. Drug released medium was collected periodically and analyzed for protein contents by micro-BCA protein assay. In order to evaluate the protein integrity, release medium was subjected to the analyses of SDS-PAGE and size exclusion chromatography (SEC). And enzyme-linked immunosorbent assay (ELISA) was introduced to measure the immunoreactivity of entrapped OVA and to get an insight into the three-dimensional structure of epitope. The structures of entrapped protein were not affected significantly by the results of SDS-PAGE and SEC. However, immunoreactivity of released antigen was varied, revealing the possibility of protein denaturation in some microspheres when it was evaluate by ELISA method. Therefore, in order to express the degree of protein denaturation, antigenicity ratio (AR) was obtained as follows: amount of immunoreactivity of OVA/total amount of OVA released ${\times}100(%)$. ELISA method was an efficient tool to detect a protein denaturation during microencapsulation and the comparison of AR values resulted in more accurate evaluation for immunoreactivity of entrapped protein.

  • PDF

Characteristics of Spray Dried Polysaccharides for Microencapsulation (미세캡슐화를 위한 분무건조 다당류의 특성)

  • Lee, Seung-Cheol;Rhim, Chae-Hwan;Lee, Sang-Chun
    • Korean Journal of Food Science and Technology
    • /
    • v.29 no.6
    • /
    • pp.1322-1326
    • /
    • 1997
  • Characteristics of viscosity and spray dried particles for several polysaccharides were studied to investigate the possibilities as wall materials for microencapsulation. Viscosities of 10% maltodextrin, 10% gum arabic, 10% dextran, 1% gum locust bean, and 1% gum karaya were 2.2 mPa.s, 9.2 mPa.s, 13.0 mPa.s, 4660.0 mPa.s, and 77.0 mPa.s, respectively. In scanning electron micrographs for spray dried polysaccharides, gum arabic had spherical shapes at 20% and 30% emulsion concentration, while trailed shapes at 40%. Maltodextrin had uniform spherical shapes at 30%, while aggregated form with various kinds of capsule sizes at 40%. Dextran had spherical shapes at 20%, while trailed fibrous shapes at over 30%. Mixed polysaccharides with gum arabic:maltodextrin (1:3, w/w) had uniform spherical shapes at 20%, 30%, and 40% with increasing diameter with increasing concentration.

  • PDF

Microencapsulation of Korean Mistletoe (Viscum album var. coloratum) Extract and Its Application into Milk

  • Kim, N.C.;Kim, J.B.;Kwak, H.S.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.21 no.2
    • /
    • pp.299-306
    • /
    • 2008
  • This study was designed to develop microencapsulated Korean mistletoe extract, to determine the stability in vitro and to examine its application in milk. Coating materials used were polyglycerol monostearate (PGMS) and medium-chain triacylglyderol (MCT). The highest efficiency of microencapsulation was 78.3% with 15:1:40 (w/w/v) as PGMS : mistletoe extract : distilled water and 66.1% with 15:1 (w/w) as MCT : mistletoe extract. The size of microcapsule was about 30.0 and $19.5{\mu}m$ with PGMS and MCT, respectively. When microcapsules of mistletoe extract were incubated in simulated gastric fluid at pH 2 for 60 min, 14.8 and 17.2% of lectin was released from capsules which were coated with PGMS and MCT, respectively. Comparatively, 83.2 and 87.3% of lectin was released in simulated intestinal fluid (pH 8) after 60 min incubation of capsules coated with PGMS and MCT, respectively. The subsequent study determined the changes of physicochemical and sensory characteristics of milk with fortification of the mistletoe extract microcapsules during 12 day storage. TBA value was significantly lower in microcapsule-added groups than in the uncapsulated mistletoe extract-added group during the storage. When 100 ppm microencapsulated mistletoe extract was added, the L-, a- and b- values and viscosity were not significantly different from those of the control. In addition, the release of lectin from mistletoe extract over 12 days was 8.3 and 9.5 mg/100 ml in milk containing microcapsules made by PGMS and MCT, respectively. All sensory attributes showed a significant difference in uncapsulated mistletoe extract-added milk compared with other groups. The present study indicated that microcapsules of Korean mistletoe extract could be applied to milk and microcapsules coated with PGMS were effectively released in a simulated intestinal environment.

Microencapsulation of Phenyl Acetate with Poly(urea-formaldehyde) (Poly(urea-formaldehyde)에 의한 페닐아세테이트의 미세캡슐화)

  • Jo, Ye-Hyun;Song, Young-Kyu;Yu, Hwan-Chul;Cho, Sung-Youl;Kumar, S. Vijay;Ryu, Byung-Cheol;Chung, Chan-Moon
    • Polymer(Korea)
    • /
    • v.35 no.2
    • /
    • pp.152-156
    • /
    • 2011
  • We have performed microencapsulation of phenyl acetate using poly (urea-formaldehyde) as a shell material, and studied the effect of agitation rate,. core/shell mass ratio, surfactant concentration, and reaction time on capsule characteristics such as size, shell thickness, and surface morphology. The formation of microcapsules was confirmed by FTIR and TGA, and capsule characteristics were studied by optical microscopy and FE-SEM. Capsule size and shell thickness reduced with increasing agitation rate. As the mass of shell material was increased, shell thickness and nanoparticles on capsule surface increased. Capsule size and shell thickness decreased with increasing the concentration of a surfactant. Increasing reaction time caused increased capsule yield and shell thickness.

Survival of Double-Microencapsulated Bifidobacterium breve in Milk in Simulated Gastric and Small Intestinal Conditions

  • Jung, Ji-Kang;Kil, Jeung-Ha;Kim, Sang-Kyo;Jeon, Jung-Tae;Park, Kun-Young
    • Preventive Nutrition and Food Science
    • /
    • v.12 no.1
    • /
    • pp.58-63
    • /
    • 2007
  • Bifidobacteria are probiotic organisms that provide both flavor and health benefits when incorporated as live cultures into commercial dairy products. Because bifidobacteria are very sensitive to environmental conditions (acids, temperature, oxygen, bile salts, the presence of other cultures, etc.), their viability in human gastrointestinal tract is limited. The microencapsulation of bifidobacteria is a process to protect them against harsh environmental conditions, thereby increasing their viability while passing through human gastrointestinal tract. To confirm the survival rate of microencapsulated Bifidobacterium breve CBG-C2 in milk, their survival rate was compared with several kinds of free bifidobacteria and lactic acid bacteria in commercial yogurt products under simulated gastric and small intestinal conditions. Double-microencapsulation of the bacteria was employed to increase the survival rate during digestion. The outer layer was covered with starch and gelatin to endure gastric conditions, and the inner layer was composed of a hard oil for the upper small intestinal regions. Almost all microencapsulted bifidobacteria in the milk survived longer than the free bifidobacteria and lactic acid bacteria in the commericial yogurt products under the simulated gastric conditions. Numbers of surviving free bifidobacteria and lactic acid bacteria in the commercial products were significantly reduced, however, the viability of the microencapsulated bificobacteria in the milk remained quite stable under gastric and small intestine conditions over 3$\sim$6 hrs. Thus double-microencapsualtion of bifidobacteria in milk is a promising method for improving the survival of bifidobacteria during the digestive process.

A comparison of anti-inflammatory activities of green tea and grapefruit seed extract with those of microencapsulated extracts (미세캡슐화한 녹차 및 자몽종자 추출물이 Murine RAW 264.7 대식세포주의 항염증에 미치는 영향 비교)

  • Jun, Yoon Kyung;Kim, Myung Hwan;Seong, Pil Nam;Chang, Moon-Jeong
    • Journal of Nutrition and Health
    • /
    • v.45 no.5
    • /
    • pp.443-451
    • /
    • 2012
  • We compared the effects of grapefruit seed extract (GFSE), green tea extract (GT) and their microencapsulated extract on anti-inflammatory activities in murine RAW 264.7 macrophages cell line. In order to protect the bioactive compounds in the extracts, they were microencapsulated with maltodextrin and $H_2O$. Nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), inducible nitric oxide synthase (iNOS) protein expression and thiobarbiturate reactive substances (TBARS) were analyzed in LPS activated RAW 264.7 macrophages. The green tea extract at the range of $100-600{\mu}g/mL$ inhibited NO, PGE2 production and iNOS protein expression without cytotoxicity in a dose-dependent manner. Grapefruit seed extract had strong inhibitory effects on NO and PGE production and iNOS protein expression at the range of $5-20{\mu}g/mL$ without cytotoxicity. Microencapsulation of green tea extract had further inhibitory effects on NO and PGE2 production and on iNOS protein expression, whereas microencapsulated GFSE did not show any further inhibitory effects on these parameters. Taken together, our results suggest that GSFE might be a promising candidate for preventing inflammation related diseases, such as cardiovascular disease, cancer or diabetes, and the microencapsulation of green tea extract could improve its bioactivity.