• 동의생리병리학회지
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• 제19권1호
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• pp.75-80
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• 2005

The purpose of study is that we will observe the change of c-fos and CGRP with the immunohistochemistry method and then we will study the effect of microcurrent stimulation following the frequency after inducing pain to rats with capsaicin. The experimental groups were divided by microcurrent application and pain induce. Normal control groups is used in experiment I, the group which we induce pain is used in experiment II, the application group which we induce pain and then the high frequency microcurrent stimulation is used in experiment III, the application group which we induce pain and then the low frequency microcurrent stimulation is used in experiment IV. c-fos was strongly expressed after pain induced 2 hours and positive neurons were decreased from 2 hours. At 7 days, positive neuron recovers to normal range, But c-fos positive neuron of microcurrent stimulation group were decreased from 2 hours. CGRP was strongly expressed after pain induced 24 hours, and positive neurons were decreased from 7 days. These results suggests that microcurrent stimulation therapy effect to control pain according to expression of c-fos and CGRP examined by immunohistochemistry. Also high frequency microcurrent stimulation is more effective than low frequency microcurrent stimulation for controling the pain.

• 한국콘텐츠학회논문지
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• 제11권10호
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• pp.286-292
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• 2011

본 연구는 토끼골절모델에 미세전류자극(직류, 음극), 고전압 맥동직류 음극과 양극을 이용해 골절치유정도를 살펴보았다. 방사선 검사에 의한 육안 계측은 미세전류자극군이 고전압 맥동직류의 음극과 양극 통전군보다 골절치유척도 점수에서 통계학적으로 유의한 차이를 보였으나(p<0.05), 고전압 맥동직류 음극군과 양극군 사이에는 유의한 차이가 없었다(p>0.05). Hematoxylin-Eosin 염색과 Masson's trichrome 염색을 통한 병리조직표본 차이는 미세전류자극군에서 무층골의 증식이 다른 두 실험군보다 더 활발하게 관찰되었으며 연골내골화 과정도 다른 두 실험군에 비해 더 빠른 것으로 관찰되었다. Osteocalcin 면역조직화학 염색은 미세전류자극군이 골모세포, 골세포, 파골세포 및 골기질 내에서 면역양성반응이 다른 두 실험군보다 더 명확히 관찰되었다.

• Physical Therapy Rehabilitation Science
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• 제12권3호
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• pp.293-299
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• 2023

Objective: This study aimed to investigate the potential benefits of microcurrent stimulation as a non-invasive therapeutic approach for managing pain and improving the quality of life in women suffering from primary dysmenorrhea. Design: A case study. Methods: This study was conducted, involving a cohort of 6 women diagnosed with primary dysmenorrhea, aged between 20 to 30 years. Participants were received microcurrent stimulation using low-intensity microcurrents for 30 minutes every day for 4 weeks. The intensity of microcurrent stimulation was 25 μA and the frequency was 8 Hz. The intervention was administered between the menstruations, with pain intensity and quality of life being assessed at baseline, and then at the end of menstrual cycle. Pain intensity was evaluated using a visual analog scale (VAS) and menstrual symptom questionnaire (MSQ), while the quality of life was assessed through the stress response inventory (SRI), state trait anxiety inventory (STAI), center for epidemiologic studies depression (CES-D), and menstrual distress questionnaire (MEDI-Q). Results: After the intervention, participants demonstrated a statistically significant reduction in pain intensity, as evidenced by improved VAS scores (p<0.05). However, the changes in MSQ scores did not reach statistical significance. Regarding quality of life measures, no statistically significant differences were found in the SRI, STAI, CES-D, and MEDI-Q scores after the intervention (p>0.05). Conclusions: This study suggest that microcurrent stimulation holds promise as a potential treatment option for alleviating pain associated with primary dysmenorrhea.

• The Journal of Korean Physical Therapy
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• 제20권1호
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• pp.67-73
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• 2008

Purpose: The purpose of this study was to investigate the effect of the intensity of microcurrent stimulation (with currents of 50 ${\mu}A$, 100 ${\mu}A$ and 300 ${\mu}A$ using a pulse frequency of 5 pps) on wound healing in rats. Methods: Sixty male Korean rats were randomly divided into four groups of 15 rats that were subjected to four different treatment protocols (control group, no treatment; experimental groups, treated with currents of 50 ${\mu}A$, 100 ${\mu}A$ or 300 ${\mu}A$). An experimental 20 mm linear wound was made in each animal and all animals in the experimental groups received microcurrent stimulation once a day for 20 minutes until the day of sacrifice on day 1, day 3 and day 6. An optical microscope was used to determine any histological changes. Results: The experimental results were as follows. 1. In an examination with the naked eye, all groups showed similar changes until the first day. However, from the third day, a little intercellular fluid soaked through wound region in the control group rats. In the experimental group animals, little intercellular fluid soaked through wound region, and swelling and redness did not appear, from the third day. 2. In an examination with histological evaluation, more significant changes were observed in all of the experimental group rats than the control group animals. Especially, a stimulation intensity of 50 ${\mu}A$ caused a more significant effect than the use of the other intensities by day 6 of wound healing. In addition, rapid recovery was observed. Conclusion: It was determined that microcurrent stimulation had a positive effect on wound healing. A stimulation intensity of 50 ${\mu}A$ was more effective than the other intensities (100 ${\mu}A$ and 300 ${\mu}A$) utilized for wound healing. Furthermore, low-intensity microcurrent stimulation was more effective for the purpose of wound healing.

• The Journal of Korean Physical Therapy
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• 제21권2호
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• pp.103-108
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• 2009

Purpose: Electrical stimulation is one of several treatments recommended for RA patients. Electrical stimulation of RA patients, reduces pain, or facilitates joint motion prior to exercises. However, there is still limited evidence on the efficacy of electrical stimulation and thus any conclusions drawn about this method remain controversial. Recently, Microcurrent Electrical Neuromuscular Stimulation (MENS) has received significant attention as a potential method of electrical stimulation. In this study, we investigated the effect of microcurrent treatment in rheumatoid arthritis rat. Methods: Subjects were allocated either to the control group or experimental group, which was subject to microcurrent stimulation. Interleukin-1 expression in the metatarsophlangeal joint and the oedema index in the ankle were used for classification and subsequent evaluation of pathology. Subjects were assessed at 1, 7 and 14 days after inducing rheumatoid arthritis through adjuvant injection. Thirty-six subjects, 18 in each group, were used in this study. Statistical analysis was performed by calculating the differences between the two groups and between each interval assessment. Categorical variables were compared between the two groups with the paired-T test. The one-way ANOVA test was performed to assess changes in ordinal variables. Results: Baseline characteristics were similar in both groups. Statistically significant differences were found between the two groups. The biological marker of pro-inflammatory cytokine and oedema index were decreased in response to this treatment. Conclusion: These data show that treatment of rheumatoid arthritis with a microcurrent stimulation device reduced the oedema index and pro-inflammatory cytokine IL-1.

• 대한임상전기생리학회지
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• 제6권1호
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• pp.43-55
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• 2008

The purpose of this study was to investigate the effect of microcurrent stimulation intensity($50{\mu}A,\;100{\mu}A,\;300{\mu}A$ - 5 pps pulse frequency was same) on wound healing in rat. Sixty male Korean rats were randomly divided into 4 groups of 15 for 4 different treatment protocols(none-control group, $50{\mu}A,\;100{\mu}A,\;300{\mu}A$ experimental groups). Experimental 20 mm linear wound were made and all animals in the experimental groups were received microcurrent stimulation once a day for 20 minutes until sacrifice days(1st day, 3rd day, 6th day). A vernier caliper was used to measure a wound healing length and an optical microscope was used to determine any histological changes. The repeated measures two-way ANOVA was used for statistical differences in wound healing length. Experimental results were as follows: 1. In the examination with the naked eye, all groups showed similar changes until 1st day. But from 3rd day, a little intercellular fluid soaked through wound region in control group. In experimental groups, little intercellular fluid soaked through wound region, and swelling and redness did not appear. 2. Wound length of experimental $50{\mu}A$ group was significantly decreased than control group(p<0.001). And in the aspect of application period, wound length was significantly decreased in 3th, 6th day than 1st day and 6th day than 3th day (p<0.001). In conclusion, it has been found that the microcurrent stimulation had a positive effect on wound healing. And $50{\mu}A$ stimulation intensity was more effective than other intensities($100{\mu}A,\;300{\mu}A$) in wound healing. Also, low-intensity microcurrent stimulation was more effective on the purpose of wound healing.

• 한국응용과학기술학회지
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• 제40권5호
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• pp.1104-1115
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• 2023

본 연구는 미세전류자극이 비만인의 체성분과 혈중지질성분의 변화에 미치는 효과를 규명하고, 복합운동의 효과와 비교함으로써 효과적인 체형 관리 방안으로서 미세전류자극의 유용성을 밝히고자 하였다. 체지방율 30% 이상인 여대생 30명을 통제집단, 복합운동집단, 미세전류자극집단으로 분류하였으며, 각 집단 별 4주간의 처치 전후 체중, 체지방율, 허리 둘레 등의 체성분 요인과 TG, TC, apolipoprotein 등의 혈중지질성분을 측정한 자료를 분석하여 다음과 같은 결과를 얻었다. 통제집단에서는 모든 측정 항목에서 통계적으로 유의한 차이가 나타나지 않은 반면 복합운동 집단에서는 허리둘레와 TC가 유의하게 감소한 것으로 나타났고, 미세전류자극 집단에서는 체중, 체지방율, 허리둘레, apolipoprotein 등이 유의하게 감소한 것으로 나타났다. 이러한 연구결과를 고려할 때 미세전류 자극은 비만인의 체성분과 혈중지질성분을 개선시켜 건강한 신체를 갖도록 하는데 효과적인 중재 방안으로 제안할 수 있다.

• 한국콘텐츠학회:학술대회논문집
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• 한국콘텐츠학회 2009년도 춘계 종합학술대회 논문집
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• pp.1124-1129
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• 2009

골절 후 골 유합에 대한 미세직류전류 자극의 효과를 관찰하기 위하여 체중 2.5~3 ㎏내외의 6개월 령 뉴질랜드 웅성토끼 24마리를 대상으로 경골 골절 후 미세전류 자극을 적용한 실험군과 비적용군인 대조군으로 나누어 3일, 7일, 14일 및 28일 후 BMP-4에 대한 면역조직화학적 염색을 실행하여 다음과 같은 결론을 얻었다. BMP-4의 발현은 비침습식 미세전류를 적용한 실험군과 자연치유군인 대조군 모두 시간이 경과함에 따라 통계학적으로 유의하게 증가하다가 감소되었다. 그러나 실험군에서 경골 골절 3일 후 대조군은 하버씨계의 간질층판을 중심으로 약한 갈색의 면역양성반응(+)을 보였으나 실험군의 경우 중등도의 면역양성반응(++)을 보였다. 경골 골절 7일 후 대조군은 하버씨계의 동심원과 간질층판을 중심으로 중등도의 갈색의 면역양성반응(++)을 보였으나 실험군의 경우 바깥층판을 포함하여 매우 강한 갈색의 면역양성반응(++++)을 보였다. 경골 골절 14일 후 대조군은 하버씨계의 간질층판과 동심원을 중심으로 강한 갈색의 면역양성반응(+++)을 보였으며 실험군 또한 강한 면역양성반응(+++)을 보였다. 그 후 점차 감소하여 경골 골절 28일 후 대조군은 하버씨계의 간질층판을 중심으로 약한 갈색의 면역양성반응(+)을 보였으나 실험군의 경우 중등도의 면역양성반응(++)을 보였다. 위의 결과로 보면 골절 후 미세전류를 비침습적으로 적용할 때 치유과정 초기에 골형성단백질인 BMP-4의 발현을 증가시켜 골절 치유를 촉진시킴을 알 수 있다.

• 대한임상전기생리학회지
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• 제9권1호
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• pp.1-8
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• 2011

Purpose : This study investigated the effects of osteoarthritis therapy using images and histological diagnosis after microcurrents were applied to osteoarthritic rats. Methods : Osteoarthritis was induced with a mixture of 2% carrageenan and 2% kaolin. The mixture (0.1 $m{\ell}$) was injected into the knee joints of rats. Osteoarthritis with articulation received microcurrent stimulation for four weeks (15 min/day, 5 days/week): treated with pulsation frequency of 5 pps and a stimulation intensity of 25 ${\mu}A$. Results : Osteoarthritis of the control group constantly changed. The group with a stimulation intensity of 25 ${\mu}A$ applied to the surface of the articular cartilage experienced near normal recovery according to image diagnosis. The result of histological and immunohistochemistry inspection confirms that microcurrent stimulation had a positive effect on the treatment of osteoarthritis. Conclusion : The differences among images and histological diagnoses show that steoarthritis will experience constant progression from stimulation.

• 대한의생명과학회지
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• 제27권2호
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• pp.99-104
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• 2021

Treatment techniques that affect homeostasis by non-invasive regulation in peripheral organs will advance disease research. Here, we demonstrate a non-invasive method of conditioning within an organ using a low-frequency stimulator superposition of alternating microcurrent wave in stages. It is first applied to the inflammatory response in H3N2-infected sinusitis mice. To check the progress of the treatment, mice were sacrificed every week for 3 weeks, nasal tissue was removed, and the inflammatory response was investigated through H & E staining. The low-frequency stimulation treatment group was found to alleviate the proliferation of epithelial cells and invasion of inflammatory cells compared to the control group as the passage of treatment time. The reduction of inflammatory cytokines in the nasal lavage fluid was observed in H3N2-infected sinusitis mice treated with of low-frequency stimulation using superposition of alternating microcurrent wave compared to H3N2-infected sinusitis mice after 3 weeks. These data demonstrate that low-frequency stimulation device in the form of using alternating current wave superposition on within organs provides a new method to regulate specific physiological functions. Therefore, it is necessary to prove the inhibitory effect of low-frequency stimulation using alternating current wave superposition on inflammatory diseases by various methods through further studies and clinical studies.