• 제목/요약/키워드: Microcantilever-type Microelectrode

검색결과 1건 처리시간 0.014초

멤스 기반의 캔틸레버 형 전극을 가진 마이크로 디바이스를 이용한 단일세포의 Electroporation 및 유전자 Transfection (Single-cell Electroporation and Gene Transfection using MEMS-based Microdevice with Cantilever-type Microelectrode)

  • 조영학;김범준
    • 한국정밀공학회지
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    • 제27권5호
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    • pp.85-91
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    • 2010
  • In this paper, we present details on fabrication of single-cell electroporation microdevice, practical experiments of single-cell electroporation with our fabricated microdevice. Also, the continuous electroporation for the continuous flow of cells is used for high-throughput electroporation. The delivery efficiency and cell viability tests are provided and the successful GFP transfection into cells is also evaluated with a fluorescent microscope after electroporation. This device enables to reduce the size of samples and thus the use of small amount of reagents. Also, it makes it possible to permit to avoid cell discrimination (transfected cells versus non-transfected cells) encountered when traditional bulk electroporation is held.