• 미생물학회지
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• 제8권2호
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• pp.69-76
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• 1970

1. The yeasts in the two samples of Nuruk (mold wheat) which one prepared at the College of Agriculture, Choong-Nam University (S) and the other purchased at a market(T), were examined and counted. The yeasts were differe entiated by the market (T), were examined and counted. The yeasts were differe entiated by the TTC(2,3,5-triphenyltetrazolium chloride)agar overlay technique that yields a varied shade of color. The results were : the population of yeasts in 1g of Nuruk S was about $6{\times}10^4$, 56.5% of which were TTC-pink yeasts, 16% TTC-red pink yeasts, 8% were TTC-red yeasts, and 16.5% TTC-white yeasts. In Nuruk T(1g), the number of yeasts accounted for $14{\times}10^4$ and constituted of 42% TTC0pink, 21% TTC-red pink, 23% TTC-red and 9% TTC-white. 2. During the fermentation of Takju (Korean Sake) employing the Nuruk S and T the yeast flora throughout the brewing were observed in 12 hour intervals. TTC pink and red yeasts considered to be the major yeasts were isolated all cultured. The strains ($1{\times}10^5$/ml) were added to the mashes S and T in which pH was adjusted to 4.2 and the change of yeast flora was examined during fermentation.

• 미생물학회지
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• 제21권3호
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• pp.163-169
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• 1983

Effects of temperature on the gorwth characteristics and the chemical composition of pseudomonas cells grown under glucose-or methanol-utilizing continuous culture were studied. In a glucose-utilizing continuous culture, optimum dilution rate, agitation, pH, and temperature, for the higher biomass yield were $0.45hr^-$, 7000rpm, pH 7.5, and $30^{\circ}C$, respectively. But in a methanol-utilizing continuous culture, they were $0.125hr^-$, 600rpm, pH 8, and $30^{\circ}C$, respectively. In methanol-utilizing continuous culture, the maximum production rate of the cells was 1.48g, dry wt./1/hr at a dilution rate of $0.45hr^-$, and the cell yield was 0.46g. dry wt./g. glucose. In the methanol-utilizaing continuous culture, the maximum production rate of the cells was 0.33 7g. dry wt./1/hr. at a dilution rate of $0.125hr^-$ and the cell yield was 0.44g dry cell/g. methanol. The contents of protein of the cells increase with the increase ingrowing temperature (from 15 to $30^{\circ}C$), more or less, while the contents of RNA nad carbohydrate of the cells decreased. However, DNA contents of cells growth under the various temperature ranges didn't change. As the temeprature of cultivation rises at a constant dilution rate, the efficiency of RNA in protein synthesis was increased, showing the decreases in the ratio of RNA to protein.

• 한국수산과학회지
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• 제48권3호
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• pp.293-300
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• 2015

We sought to extend the shelf-life of Alaska pollack Theragra chalcogramma sikhae while maintaining quality. We compared the chemical, microbiological, and organoleptic characteristics of sikhae prepared under four different conditions. Control fish were fermented at ambient temperature ($21{\pm}2^{\circ}C$); other samples were stored at $5^{\circ}C$ after fermentation at ambient temperature for 36 h (A1), 60 h (A2), and 84 h (A3). Volatile basic nitrogen and amino-nitrogen levels, and total acidity increased with fermentation time in all samples, but the pH fell, attaining a relatively lower level in the control than in other samples. Over 90% of all viable cells were lactic acid-producing bacteria; this proportion did not change significantly during fermentation. In terms of texture, only hardness was affected by fermentation. The hardness of the control fell more rapidly than did that of the other samples. In terms of sensory evaluation (the acceptance test and quantitative descriptive analysis [QDA]), A2 was superior to other samples after fermentation for different times; A2 maintained limited salability (6 points on the relevant index) for up to 17 days of storage.

• 한국수산과학회지
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• 제51권5호
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• pp.477-490
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• 2018

This study investigated the changes in the quality of doenjang (a fermented bean paste) with the addition of Sea tangle Saccharina japonica powder fermented by lactic acid bacteria (FSP). Doenjang was prepared without or with FSP and with various amounts of doenjang (0, 0.25, 0.5, 1 and 3% by volume), and fermented at $25^{\circ}C$ for 12 weeks. After 12 weeks, the antioxidant activity, microbiological characteristics, and physicochemical parameters were examined. We detected no significant differences in the color profiles. However, following the addition of FSP to doenjang, the amino nitrogen content and antioxidant properties were enhanced. Furthermore, the FSP-doenjang contained higher levels of ${\gamma}$-aminobutyric acid (297.55-300.03 mg/100 g), a biofunctional ingredient. These results suggest the potential for developing a traditional Korean fermented food with enhanced antioxidant activity and ${\gamma}$-aminobutyric acid content using FSP.

• KSBB Journal
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• 제32권1호
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• pp.46-53
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• 2017

There are four types (So-Eum, So-Yang, Tae-Eum, Tae-Yang type) in Sasang constitutional medicine (SCM). In SCM, not only people but also herbal medicine are classified into four types. To date, there has been very little evidence of experimental classifications in SCM. So, we adapted Issatchenkia orientalis in two different culture media made from the herbal medicines for So-Eum and for So-Yang type. And the adapted Issatchenkia orientalis was inoculated into another eight culture media made from the herbal medicines for So-Eum and for So-Yang type. The mass reduction was measured every 12 hours for 2 days. Then, we analyzed the statistical differences of herbal medicines for So-Eum and for So-Yang type. There were significant differences of variables (R: rate of change in mass reduction, S: convex degree of mass reduction graph) in herbal medicine media for So-Eum (p=0.001, 0.001, respectively.). Suggesting a microbiological method of Sasang medicine research, current study may contribute to the establishment of experimental Sasang constitutional herbal medicine.

• Preventive Nutrition and Food Science
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• 제12권4호
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• pp.284-288
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• 2007

Effect of aqueous chlorine dioxide $(ClO_2)$ treatment on the quality change of fresh ginseng during storage was examined. Fresh ginseng samples were treated with 0, 50, and 100 ppm of $ClO_2$ solution, respectively, and stored at $4^{\circ}C$. Microbiological data of the fresh ginseng after $ClO_2$ treatment revealed that the populations of total aerobic bacteria, and yeast and mold were significantly reduced with the increase of $ClO_2$ concentration. In particular, the populations of total aerobic bacteria, and yeast and mold in the fresh ginseng decreased by 2.1 and 1.2 log CFU/g at 100 ppm $ClO_2$ treatment, respectively. Aqueous $ClO_2$ treatment improved the color of the fresh ginseng during storage, but there was no significant difference in weight loss during storage among treatments. Sensory evaluation results represented that the qualities of the fresh ginseng treated with aqueous $ClO_2$ during storage were better than those of the control. These results clearly indicate that aqueous $ClO_2$ treatment could be useful in decreasing the microbial growth and extending the shelf life of fresh ginseng.

• Journal of Microbiology
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• 제33권4호
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• pp.328-333
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• 1995

$\^$31/ P-NMR experiment was performed to detect phophonates (Pn) utilization and degradation in the several different C-P lyase mutants of E. coli and in E. aerogenes and the recombinants. The relative peak intensity (RPI) for the standard samples of 0.5 mM methylphosphonate (MPn) and 1.0 mM aminoethylphosphonate in glucose-MOPS medium showed 0.5 : 1.0 ratio. In the case of BW14329 (.DELTA.phnC-P, .delta.phoA), RPI did not change significantly after 24 hrs culturing, which means it nearly could not utilize Pn. In vivo $\^$31/ P-NMR spectrum of E. aerogens (BWKL 16627) during 3 hrs starvation showed two intense peaks at 0-2 ppm and at near-10 ppm which indicate intracellular orthophosphate (Pi) and pyrophosphate (PPi), respectively. Both of them might be released by degradation of inorganic polyphosphate pool. When MPn is supplied to the medium as an unique P source, Pi content in the cell has the constant, but PPi seems to be slightly decreased. Recombinants (BWKL 16954) grew slower than E. aerogenes in the glucose-MOPS media with various P sources. In vivo $\^$31/ P-NMR spectrum of recombinant did not show any intense signal in the cell. Surprisingly, under the cultivation adding with MPn, a few intense peaks in the region of Pi AND phospate monoester were detected.

• 미생물학회지
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• 제8권1호
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• pp.13-20
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• 1970

Chenges in respiration of the soils collected from the reclaimed tidal soil in Chogi-ri, Kanghwa Island and treated with organic matters are presented. The measurement of the respiration for the smaples, which were incubated for 0,2,4 and 5 weeks, were carried out by using Warburg's respirometer. While the respirations of the samples added by organic matters were increased 6.7 - 28.0 times compared with that 0 week during the incubation in case of 5-year soil, the respiration in case of 3-year soil were increased 3.3-11.8 times. Thus, the effect of adding organic matters on the respiration of the experimented soils, as this indicates, was much higher for the soil of 5-year area than that of 30-year area. And for the organic matters Salicornia was most effective and then Suaeda and Oryzae. The samples treated with Salicornia and Suaeda showed their highest respiration rate at the 4th week, but the one with Oryzae was measured to increase progressively during 5 weeks experimented. Regarding the salinity, content of organic matters and number of bacteria, in each intact soil experimented, 5-year soil samples had much poor habitat then 30-year soil for the activity of soil microorganisms, but according to the result mentioned above, it is firmly believed that the addition of organic matters on the saline soil is one of the best means to change the reclaimed tidal land into arable land with less time duration.

• 한국초지조사료학회지
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• 제41권4호
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• pp.302-307
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• 2021

In the current study, lactic lactic acid bacteria (LAB) Lactobacillus plantarum and Pediococcus pentosaceus were used as a mixed additive for the production of Orchardgrass silage by ensiled method and nutritional change fermentation ability and microbial content of experimental silages. The addition of LAB to Orchardgrass during ensiling process rapidly reduced the pH of the silages than the non-inoculated silages. In addition, the lactic and acetic acid content of silage was increased by LAB strains than the non-inoculated silages whereas butyric acid content was reduced in silage treated with LAB. A microbiological study revealed that higher LAB but lower yeast counts were observed in inoculated silages compared to non-inoculated silage. Overall data suggested that the addition of LAB stains could have ability to induce the fermentation process and improve the silage quality via increasing lactic acid and decreasing undesirable microbes.

• 미생물과산업
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• 제19권2호
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• pp.49-57
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• 1993

Chenges in respiration of the soils collected from the reclaimed tidal soil in Chogi-ri, Kanghwa Island and treated with organic matters are presented. The measurement of the respiration for the smaples, which were incubated for 0,2,4 and 5 weeks, were carried out by using Warburg's respirometer. While the respirations of the samples added by organic matters were increased 6.7 - 28.0 times compared with that 0 week during the incubation in case of 5-year soil, the respiration in case of 3-year soil were increased 3.3-11.8 times. Thus, the effect of adding organic matters on the respiration of the experimented soils, as this indicates, was much higher for the soil of 5-year area than that of 30-year area. And for the organic matters Salicornia was most effective and then Suaeda and Oryzae. The samples treated with Salicornia and Suaeda showed their highest respiration rate at the 4th week, but the one with Oryzae was measured to increase progressively during 5 weeks experimented. Regarding the salinity, content of organic matters and number of bacteria, in each intact soil experimented, 5-year soil samples had much poor habitat then 30-year soil for the activity of soil microorganisms, but according to the result mentioned above, it is firmly believed that the addition of organic matters on the saline soil is one of the best means to change the reclaimed tidal land into arable land with less time duration.