• 한국포장학회지
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• 제7권1호
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• pp.25-30
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• 2001

The purpose of this study was to examine how can we keep the freshness of peeled chestnuts in distribution process. The 30 minutes impregnation treatment at the preservation solution of 0.3% consistency showed a good result. The solution treatment prevented from surface yellowing and quality degradation of peeled chestnuts. And, in vaccum packaging after treating chemicals surface drying was faster than the other packaging from 7th day. A vitamin C treatment was no effective to restrain the growth of microbials.

• Asian-Australasian Journal of Animal Sciences
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• 제31권11호
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• pp.1781-1794
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• 2018

Objective: This meta-analysis was conducted to evaluate the overall effect of direct-fed microbial (DFM) or probiotic supplementation on the log concentrations of culturable gut microbiota in broiler chickens. Methods: Relevant studies were collected from PubMed, SCOPUS, Poultry Science Journal, and Google Scholar. The studies included controlled trials using DFM supplementation in broiler chickens and reporting log concentrations of the culturable gut microbiota. The overall effect of DFM supplementation was determined using standardized mean difference (SMD) with a random-effects model. Subgroups were analyzed to identify pre-specified characteristics possibly associated with the heterogeneity of the results. Risk of bias and publication bias were assessed. Results: Eighteen taxa of the culturable gut microbiota were identified from 42 studies. The overall effect of DFM supplementation on the log concentrations of all 18 taxa did not differ significantly from the controls (SMD = -0.06, 95% confidence interval [-0.16, 0.04], p = 0.228, $I^2=85%$, n = 699 comparisons), but the 18 taxa could be further classified into three categories by the direction of the effect size: taxa whose log concentrations did not differ significantly from the controls (category 1), taxa whose log concentrations increased significantly with DFM supplementation (category 2), and taxa whose log concentrations decreased significantly with DFM supplementation (category 3). Category 1 comprised nine taxa, including total bacterial counts. Category 2 comprised four taxa: Bacillus, Bifidobacterium, Clostridium butyricum, and Lactobacillus. Category 3 comprised five taxa: Clostridium perfringens, coliforms, Escherichia coli, Enterococcus, and Salmonella. Some characteristics identified by the subgroup analysis were associated with result heterogeneity. Most studies, however, were present with unclear risk of bias. Publication bias was also identified. Conclusion: DFM supplementation increased the concentrations of some beneficial bacteria (e.g. Bifidobacterium and Lactobacillus) and decreased those of some detrimental bacteria (e.g. Clostridium perfringens and Salmonella) in the guts of broiler chickens.

• 환경위생공학
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• 제3권1호
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• pp.27-39
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• 1988

This investigation was carried out to evaluate the removal effect of biological contaminants for the municipal sewage treatment process at Cheonggye Cheon terminal plant which in the first plant for municipal sewage treatment in Seoul area. It was conducted in raw influent, primary treatment water and secondary treatment water from September, 1986 to July, 1987. The results were as follow; 1, The primary treatment could eliminate microbials for $65.38\%$ of total bacteria, $64.35\%$ of total coliform, $62.16\%$ of fecal coliform $69.48\%$ of pseudomonas and $64.70\%$ of fecal streptococci in averages for a year respectively. 2. The secondary treatment could eliminate microbials for $97.50\%$ of total bacteria, $97.30\%$of total coliform, $95.95\%$ of fecal coliform, $97.00\%$ of pseudomonas and $96.53\%$ of fecal streptococci in average for a year respectively. 3. In the detect rate of pathogenic agent, salmonella spp was decreased $12.5\%$ to $4.2\%$ in primary treatment and it was not detected in secondary treatment, shigella spp was detected $4.2\%$ in influent water but it was not detected in primary and secondary treatment. 4. In the seasonal variation of treatment effect, the removal of summer was the highest, and the removal of all item in winter was lower than the other seasons. 5. There was significant correlation between water temperature and microbal all items (P<0.05) $NH_3-N$ and Microbal items (P< 0.01) at raw water.

• 한국응용과학기술학회지
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• 제30권3호
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• pp.411-422
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• 2013

Polymers in cosmetics are used to deliver desired attributes to skin and hair. Precisely constructed block and graft copolymers widen the range of available mechanical properties and compatibilities. Stimuli responsive hydrophobic polymers can be triggered to become hydrophilic by changes in their environment and this can confer waterproof properties at low temperature and easy water removal at higher temperatures. Transfer-resistant cosmetics can be possible due to silicone resins. The control of rheology properties in cosmetics gradually continue to be easy with copolymers. Durability of colors and fragrances for rinse-off products can be enhanced by delivery systems from complex coacervates. Polymeric anti-microbials promise product preservation while minimizing the concern of skin permeation. This article reviews recent trends in the use of polymers in cosmetics.

• Journal of Life Science
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• 제9권2호
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• pp.57-61
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• 1999

This study was designed to develope the antioxidative activity on oxidation of human low density lipoprotein(LDL) from marine microbials. Bacillus KS-96 producign antioxidant have been isolated and identified from seawater, Bacillus sp. KS-96. The optimal medium pH was 7.0 and incubation temperature was 30$^{\circ}C$. The antiosidant of potential substance produced extracellularly in the culture broth by Bacillus sp. KS-96 was obtained by elution of silica gel culumn chromatography with hexane, ethylacetate and water. The ethylacetate faction are shown at highest level of antioxidant activity using thiocyanate method among them. By IR, NMR, and GC/MS, antioxidant purified from ehtylacetate fraction was identified and named as 2,6-dimethoxyphenol. 2,6-dimethoxyphenol inhibited the metal mediated oxidation of human LDL at concentration of 50∼100 ${\mu}$g/mL in the presence of 5uM CuSO4 with macrophage or J774 cells.

• Natural Product Sciences
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• 제9권3호
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• pp.183-185
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• 2003

The combination of hexane extract (E4) of rhizome of Acorus gramineus with chloramphenicol (Cm) was applied to Gram negative Cm resistant microbials to find the possibility of clinical use and to clarify the relationship of the activity of chloramphenicol acetyltransferase (CAT). The combination of $1,000\;{\mu}g/ml$ of E4 and $8\;{\mu}g/ml$ of Cm entirely ceased the growth of S. aureus SA2, a gram positive resistant strain to 10 antibiotics. But in Gram negative strains which possess CAT activity, some showed considerably strong resistances to Cm and some did weakly.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1998년도 Proceedings of UNESCO-internetwork Cooperative Regional Seminar and Workshop on Bioassay Guided Isolation of Bioactive Substances from Natural Products and Microbial Products
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• pp.131-131
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• 1998

In the course of screening for new natural compounds having antioxidant activity from microbials, mushrooms as well as plants. We have isolated novel sesquiterpenoid and officinalic acid from methanolic extracts of Stereum ostrea. The dried fruit body( 450 g) of Stereum ostrea was extracted with methanol, then methanolic extract was partitioned between water and hexane, chloroform, and ethyl acetate, subsequently. The chloroform extract was subjected on silica gel, Sephadex LH-20 and RP-18 column chromatography, successively. And two compounds were isolated and identified as new triterpenoids and officinalic acid using $^1$H - NMR, $\^$13/C - NMR as well as Mass spectra.

• Journal of Animal Science and Technology
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• 제47권5호
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• pp.789-804
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• 2005

본 연구는 액상 DFM(Bacillus spp.)을 완전혼합사료(실험 1) 및 다양한 비율의 starch와 cellulose에 적용(실험 2) 하였을 때, 반추위내 발효와 섬유소분해효소활성에 미치는 영향을 평가하기 위해 in vitro 배양을 24시간동안 실시하였다. 실험 1의 처리구는 완전혼합사료에 액상 DFM 0%(대조구), 0.025%, 0.05%로 각각 첨가하였다. 실험 1에서, 0.025%구는 배양 6, 9시간에서 대조구와는 유의한 차이가 없었으나, 0.05%구보다는 유의하게 낮은 결과를 보였다(P<0.05). 암모니아질소농도와 휘발성지방산농도는 액상 DFM에 의하여 영향을 받지 않았다. 하지만, A:P비율은 DFM의 적용으로 배양 24시간에 대조구에 비하여 0.05%구에서 유의한 증가를 나타내었다(P<0.05). 액상 DFM적용에 의한 섬유소분해효소활력은 전반적으로 유의한 효과가 나타나지 않았지만, CMCase는 배양 6시간에 대조구에 비하여 유의한 증가를 나타내었다(P<0.05). 하지만, xylanase는 배양 12시간의 0.05%구에서 오히려 액상 DFM의 적용으로 유의하게 감소하였다(P<0.05). 반추위내 건물소실율은 액상 DFM의 첨가수준에 의해 유의한 차이가 나타나지 않았다. 실험 2는 다양한 비율(90:10, 70:30, 50:50, 30:70, 10:90)의 starch와 cellulose에 액상 DFM을 각 비율에 대하여 대조구(0%)와 첨가구(0.025%)로 나누어 반추위액을 이용하여 배양하였다. 반추위내 pH는 액상 DFM의 첨가로 유의한 영향을 받지 않았고, starch의 비율이 증가함에 따라 부분적으로 유의하게 감소하였다(P<0.05). 암모니아질소농도는 액상 DFM의 첨가와 starch 및 cellulose의 비율에 의하여 유의한 영향을 받지 않았다. 하지만, 배양 9시간의 70:30비율에서 액상 DFM첨가구가 35.65mg/dL의 암모니아질소농도를 나타내어 대조구의 65.05mg/dL에 비하여 유의하게 감소하였다(P<0.05). 휘발성지방산농도는 70:30비율의 6시간 배양에서 액상 DFM구가 대조구에 비하여 유의한 증가를 나타내었다(P<0.05). 액상 DFM의 첨가는 CMCase에는 전반적으로 유의한 영향을 미치지 않았다. 그러나 xylanase에는 90:10, 30:70 및 10:90구에서 부분적인 유의성이 인정되었다. 본 연구결과로부터, 액상 DFM의 적용은 in vitro 발효성상과 섬유소분해효소활성을 유의적으로 향상시키지 않았고, 사료의 조성에 따라 그 효과는 다양하게 나타나 일관성이 없는 것으로 평가되었다.

• 한국식품과학회지
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• 제42권3호
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• pp.281-286
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• 2010

본 연구는 자동화된 상업용 배추김치 제조공정 중에서 원료와 공정별로 미생물의 변화를 조사하고자 수행하였다. 김치제조에 사용되는 여러가지 재료별로 미생물 분포를 조사한 결과, 마늘, 생강, 고추가루에서 많은 수의 호기성 박테리아, 젖산균, Leuconostoc, 그리고 효모가 검출되었으며, 이로써 위 부원료들이 김치에 존재하는 미생물들의 주요 공급원임을 알 수 있었다. 김치를 제조하는 공정 별로 미생물 변화를 모니터링 한 결과, 배추절임 공정에서 총 균수가 증가하였고 그 이후 세척공정에서는 단지 미생물수를 1 log CFU/g 줄이는데 그쳐 미생물 수 감소효과는 적은 것으로 판명되었다. 효모 또한 다양한 부재료와 최종 김치에서 검출되었다. 따라서, 김치 제품에 최종적으로 존재하는 미생물 수를 줄이고자 한다면 위의 원료 중에서는 위의 부재료에 대해, 그리고 제조공정에서는 절임에 사용되는 천일염과 절임탱크에 대해 적절한 살균대책이 강구되어야 하겠다. 또한 차염소산 살균 공정은 절임과 세척공정 사이에 위치해야만 가장 큰 효과를 기대할 수 있을 것으로 판단되었다. 또한 검출된 효모에 대한 저해 수단으로 적절한 저해제의 첨가도 고려되어야 하겠다. 본 연구의 결과는 공장김치의 HACCP 구축과 개선에 활용될 것으로 기대된다.

• Asian-Australasian Journal of Animal Sciences
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• 제23권12호
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• pp.1657-1667
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• 2010

Direct-fed microbials (DFM) are dietary supplements that inhibit gastrointestinal infection and provide optimally regulated microbial environments in the digestive tract. As the use of antibiotics in ruminant feeds has been banned, DFM have been emphasized as antimicrobial replacements. Microorganisms that are used in DFM for ruminants may be classified as lactic acid producing bacteria (LAB), lactic acid utilizing bacteria (LUB), or other microorganisms including species of Lactobacillus, Bifidobacterium, Enterococcus, Streptococcus, Bacillus and Propionibacterium, strains of Megasphaera elsdenii and Prevotella bryantii and yeast products containing Saccharomyces and Aspergillus. LAB may have beneficial effects in the intestinal tract and rumen. Both LAB and LUB potentially moderate rumen conditions and improve feed efficiency. Yeast DFM may reduce harmful oxygen, prevent excess lactate production, increase feed digestibility, and improve fermentation in the rumen. DFM may also compete with and inhibit the growth of pathogens, stimulate immune function, and modulate microbial balance in the gastrointestinal tract. LAB may regulate the incidence of diarrhea, and improve weight gain and feed efficiency. LUB improved weight gain in calves. DFM has been reported to improve dry matter intake, milk yield, fat corrected milk yield and milk fat content in mature animals. However, contradictory reports about the effects of DFM, dosages, feeding times and frequencies, strains of DFM, and effects on different animal conditions are available. Cultivation and preparation of ready-to-use strict anaerobes as DFM may be cost-prohibitive, and dosing methods, such as drenching, that are required for anaerobic DFM are unlikely to be acceptable as general on-farm practice. Aero-tolerant rumen microorganisms are limited to only few species, although the potential isolation and utilization of aero-tolerant ruminal strains as DFM has been reported. Spore forming bacteria are characterized by convenience of preparation and effectiveness of DFM delivery to target organs and therefore have been proposed as DFM strains. Recent studies have supported the positive effects of DFM on ruminant performance.