• Title/Summary/Keyword: Microbial number

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Estimation of the Number of Salmonellosis Using Microbial Risk Assessment Methodology (미생물 위해성 평가 방법을 이용한 살모넬라 발생수 추정)

  • 최은영;박경진
    • The Korean Journal of Community Living Science
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    • v.15 no.2
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    • pp.167-177
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    • 2004
  • The number of foodborne salmonellosis was estimated by using microbial risk assessment(MRA) methodology and the possibility of application was studied through comparison with previous results. The contamination levels of Salmonella sp. were estimated by using published domestic studies(1997∼2000) and monitoring data (1999∼2001) from food-safety related institutes. Data on food consumption came from the 2001 National Health and Nutrition Survey, and dose-response models from studies in other countries. Simulation results showed that there were 753,368 cases of salmonellosis in Korea in 1 year, which is about 115 times that reported in previous years and lower than the WHO's estimation increase. From these results, microbial risk assessment is likely to be available for estimation of the number of foodborne illnesses and determination of the order of priority in food-safety management. Butthe verification methods are not established and most of the data on contamination levels of foodborne bacteria, food consumption, and dose-response relationships have not been established. In addition, the actual conditions of circulation, storage and cooking must be studied further.

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Total Coliforms and Fecal Coliforms as Microbial Indicators of Waterborne Enteric Viruses in Korean Surface Water (한국 지표수에서 수인성 장관계 바이러스에 대한 지표 미생물로서 총 대장균군과 분원성 대장균군)

  • Lee, Gyucheol;Lee, Heesuk
    • Journal of Korean Society of Water and Wastewater
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    • v.20 no.6
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    • pp.885-892
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    • 2006
  • In order to investigate whether or not Total Coliforms (T.C.) and Fecal Coliforms (F.C.) are compatible as indicator microorganisms of waterbome enteric viruses, a total of 192 surface water samples from 24 locations in Korea were tested for T.C., F.C., and human enteric viruses from July 2003 to January 2006. Altogether, the number of T.C. in each samples was ranged from $0{\sim}5.3{\times}10^4$ colony forming unit(CFU)/100mL, and the number of F.C. ranged from $0{\sim}5.0{\times}10^3CFU/100mL$ per sample. Thirty-three percent of the samples tested positive for human enteric viruses after the total culturable virus assay. The results of the statistical analysis showed that T.C. and F.C. had a significant correlation with turbidity and temperature, but the waterbome enteric viruses did not. When compared to the number of T.C. or F.C. per sample, the concentration of waterbome enteric viruses was not found to be correlated. In conclusion, it is suggested that T.C. and F.C. may not be sufficient microbial indicators of waterbome enteric viruses in the samples analyzed in this study. However, further research is needed to find other microbial indicators of waterbome enteric viruses and to develop more advanced and sensitive methods to detect waterborne enteric viruses.

Systematic Analysis of Microbial Contamination in Leaf and Stem Products in Korea (Systematic analysis 방법을 이용한 국내 엽경채류 농산물의 미생물학적 오염도 분석)

  • Sung, Seung-Mi;Min, Ji-Hyeon;Kim, Hyun Jung;Yoon, Ki-Sun;Lee, Jong-Kyung
    • Journal of Food Hygiene and Safety
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    • v.32 no.4
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    • pp.306-313
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    • 2017
  • This study systemically analyzed the data on the microbial levels in fresh vegetables in Korea to identify the points to control. We scanned the studies published between 2001 and 2015 in peer-reviewed research papers on the microbial levels in fresh vegetables produced in Korea. Plant products were categorized by using the US IFSAC (Interagency Food Safety Analytics Collaboration) category. The most consumed, the non-heat treated, the epidemiological foodborne diseases sources of fresh vegetable in foodservice (KCDC data) were identified by literature review. Articles were screened using National Digital Science Library (NDSL) search engine regarding to microbial hazards in plant products. Based on the total plate count number and coliforms on the 89 data cases from 26 published articles, the total plate count number was high in the order of sprouts, leaf and stem, bulbs and roots, vine-grown, solanaceous, melons, and pome. Escherichia coli was frequently detected in leaf and stem and sprouts products. Focused on the microbial data of leek, lettuce and cabbage, the levels of total plate count, coliforms and Bacillus cereus showed the levels of 4.15~7.69 log CFU/g, 1~6.99 log CFU/g, and 0.51~3.9 log CFU/g, respectively, by 33 published papers. The levels of environmental factors affecting the microbial safety of lettuce and leek before harvest were investigated. Manure, soil, hands, scale, gloves were the major potential microbial contamination points to control. In addition, GAP (good agricultural practice), microbial testing, and improvement of irrigation methods are required to provide the safer fresh produce.

토양경작법을 이용한 유류오염 토양 복원시 미생물제제 적용 효과

  • 차재욱;염규진;박영준;김선미;최기창;이문현;박광진;김정철
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2004.04a
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    • pp.39-42
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    • 2004
  • Landfarming and a microbial agent, Bioil-D, were used to treat diesel-contaminated soil. The microbial agent was applied to the contaminated soil in a concentration of 5$\times$10$^2$ cfu/soil(g) and the total amount of microbial agent, 210$\ell$, was sprinkled on the soil four times for 24 days during 50 day-remediation period. The remediation goal, lower than 800mg/kg of TPH, was achieved from 5, 707 mg/kg of TPH within 50 days. The total number and activity of indigenous microorganisms were increased by 100 times after the microbial agent, Bioil-D, was applied to the contaminated soil.

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토양경작법을 이용한 유류오염 토양 복원시 미생물제제 적용 효과

  • 환경보전협회
    • Bulletin of Korea Environmental Preservation Association
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    • v.26 no.5_6
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    • pp.59-61
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    • 2004
  • Landfarming and a microbial agent were used to treat dilsel-contaminated soil. The microbial agent was applied to the contaminated soil in a concentration of 5$\times$102 cfu/soil(g) and the total amount of microbial agent, 210 l , was sprinkled on the soil four times for 24 days during 50 day-remediation period. The remediation goal, lower than 800mg/kg of TPH, was achieved from 5,707mg/kg of TPH within 50 days. The total number and activity of indigenous microorganisms were increased by 100 times after the microbial agent, Bioil-D, was applied to the contaminated soil.

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On the Decay Rate of Soil Organic Matter and Changes of Soil Microbial populaiton (토양유기물의 분해속도와 Microbial populaiton의 소장에 관한 연구)

  • 김춘민
    • Journal of Plant Biology
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    • v.10 no.1_2
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    • pp.21-30
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    • 1967
  • The aim of present study is to elucidate the relationship between decay rate of soil organic matter, and the change of soil microbial population under the oak and pine forest soils in Kwang-nung plantation stand. The results obtained are as follows: 1) The correlation coefficient between decay rate and the soil bacteria is 0.84 and fungi 0.93. 2) The distribution of soil microbial population is higher in both F horizon of the oak forest soil, and F and H horizon of the pine forest soil. However, the number of soil microorganisms decreases with the depth in each forest soil. 3) The population of soil microbes is related to moisture content, total nitrogen, available phosphorus, and exchangeable calcium, except organic carbon in fungi. 4) The soil organic matter has been mainly decomposed by fungi, and the size of its population are governed by the factors such as moisture content, organic carbon, total nitrogen, available phosphorus, and exchangeable calcium.

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Studies on Development of New Basidiomycetes by Protoplast Fusion and Nuclear Transfer II - The Effects of the Components of the Protoplast Fusants on Mouse Immune Cells - (원형질체 융합 및 핵전이에 의한 새로운 담자균류의 개발에 관한 연구(II) - 융합균사체의 항암성분이 생쥐의 면역세포에 미치는 영향 -)

  • Moon, Chul;Kim, Chae-Kyun;Yoon, Jong-Myung;Shim, Mi-Ja;Kim, Ha-Won;Choi, Eung-Chil;Kim, Byong-Kak
    • Korean Journal of Pharmacognosy
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    • v.27 no.3
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    • pp.231-237
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    • 1996
  • The antitumor components of the protoplast fusants of Lentinula edodes and Ganoderma lucidum were examined for immunological activity to elucidate the mechanism of their antitumor activity. They did not show any direct cytotoxicity against tumor cells. But being examined for immunopotentiation activity, they increased the number of colonies in the bone marrow stem cells to 3.0 times. They also increased the activities of the acid phosphatase in activated macrophages to 2.1 times and the secretion of nitric oxide in RAW 264.7 to 2.2 times, respectively. They activated the components of the alternative complement pathway. In humoral immunity. they increased the activities of the alkaline phosphatase in differentiated B cells to 1.6 times and the number of plaque forming cells to 1.8 times, respectively. In cellular immunity, they restored the depressed response of delayed type hypersensitivity in tumor bearing mice to normal level.

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Microbial Community Changes in the Soil of Plastic Film House as Affected by Anaerobic Fermentation of Rice Bran or Wheat Bran (쌀겨와 밀기울의 토양 혐기발효 처리가 시설 재배지 토양의 미생물상에 미치는 영향)

  • Kim, Hong-Lim;Weon, Hang-Yeon;Sohn, Bo-Kyun;Choi, Young-Hah;Kwack, Young-Bum
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.5
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    • pp.341-347
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    • 2009
  • Soil microbial community has been changed after the treatment of anaerobic fermentation using wheat bran or rice bran was applied to the soil. In the dilution plate technique, the number of anaerobic bacteria and fungi was higher in rice bran-treated soil than in non and wheat bran-treated soil, but of yeast was higher in wheat bran-treated soil than in non and rice bran-treated soil. Specially, the fungi were not detected in the wheat bran-treated soil. Identified by 16S rDNA sequencing, the number of aerobic bacteria was similar in all treatments, the dominant bacteria was the genus Bacillus. In the phospholipid fatty acid (PLFA) technique, both Gram-positive and Gram-negative bacteria change slightly in all treatments for 20 days of fermentation process but, after 20day, increased rapidly in wheat or rice bran-treated soil. In conclusion, the microbial communities structure was dramatically changed after the treatment of wheat or rice bran to soil.

Effect of Soil Microbial Fertilizers on Yield of Chinese Cabbage (Brassica campestris L.) (토양미생물제처리(土壤微生物劑處理)가 배추의 수량(收量)에 미치는 영향(影響))

  • Kim, Kyung-Je;Kim, Seog-Kyun
    • Horticultural Science & Technology
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    • v.16 no.3
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    • pp.341-343
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    • 1998
  • This study was carried out to investigate the effects of soil microbial fertilizers on yields of Chinese cabbage (Brassica campestris L.). Five microbial fertilizers, MPK+Husk+Palma, Husk+Palma, MPK+Compost, BLCS(Bio livestock cattle system) cattle dropping, and Tomi, were used. All of microbial fertilizers significantly increased yields of Chinese cabbage, except BLCS cattle dropping. MPK+Husk+Palma was the most effective than any other treatments. Chinese cabbage treated with Tomi showed higher concentrations of K, Ca, Mg, Fe, Mn, and Zn than Compost treatment. MPK+ Husk+Palma was high in concentrations of Mg and Mn. MPK+Compost was high in concentrations of K, Mg, and Na. In a chemical components of soil, concentrations of K and P was increased with Tomi treatment, however, the other concentrations of plant and soil chemical components were not different. In a microbial properties of soil, Tomi, Husk+Palma, and MPK+Husk+Palma treatments increased in the number of total bacteria and bacilli. Tomi treatment increased in the number of actinomycetes and fungi. The other microbial properties of soil showed no significant differences. It would be assumed that yield increase in Chinese cabbage might be due to the beneficial microbial properties, therefore, those would increase yields of Chinese cabbage.

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Effect of Microbial Fermentation Compost by Cultivating Area (栽培地域 差異에 따른 微생物 발효퇴비의 施용效果)

  • 이종성;정원복;정순재;오주성;강경희;김회태
    • Korean Journal of Organic Agriculture
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    • v.10 no.1
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    • pp.87-97
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    • 2002
  • This study was conducted to investigate the effect of microbial fermentation compost on the growth, yields and components of Altari Radish, and the soil physicochemistry property by cultivating area. microbial fermentation compost, 90kg/10a, 180kg/10a, 270kg/10a, chemical fertilizer plot were used. The results were summarized as follows ; Change of soil microbial after application microbial fermentation compost, The number of bacteria and actinomycetes in developed land showed the increased tendency. The application effects of microbial fermentation compost on the growth and yield of Altari radish were increased by excessive application with all kinds of microbial fermentation compost, especially, treatment plot at 270kg/10a was the highest. Change of components in Altari Radish was not different between treatments.

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