• 한국식품과학회지
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• 제38권1호
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• pp.28-34
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• 2006

연구에서는 세정 및 표면살균처리 시스템을 개발 제작에 의한 세정, 표면살균처리 및 저장효과를 살펴보기 위해 치커리를 시료 로 수확후 저장기간별 품질평가를 실시하였다. 표면살균처리를 위해 오존수와 염소수를 사용한 결과 3 ppm 오존수와 염소수 100 ppm의 표면살균 처리는 미생물적 결과에서 커다란 차이를 보이지 않았으나, 두 살균수 모두 식품가공에 있어 제균력을 가지고 있음을 확인 할 수 있었다. 처리 조건을 신선편이 치커리에 적용해본 결과 저장 조건에 있어서는 $4^{\circ}C$에서 시료들은 관능적 평가에 있어서 9일까지는 양호한 상태를 보였으나, 이화학적 특성인 총비타민C와 총클로로필 함량을 시험해보았을 때, 영양소적 손실을 보였고, $10^{\circ}C$에 저장한 치커리의 경우 6일 이후 급격히 상품가치를 잃었다. 본 연구 결과를 토대로 위생적이고 안전한 최 소가공 농산물의 생산을 위하여 기계세정을 이용한 공정시간의 단축과 살균과정을 이용하여 식품 안전성의 위험이 될 수 있는 미생물의 오염을 막을 수 있고, 오존수를 이용하여 염소수 소독에 의존하고 있는 일반적인 생산과정의 중점관리로 지적되었던 사항을 개선할 수 있는 새로운 위생관리 방안으로써 이용 가치 있다고 평가된다.

• Journal of Periodontal and Implant Science
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• 제49권5호
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• pp.319-329
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• 2019

Purpose: Direct application of atmospheric-pressure plasma jets (APPJs) has been established as an effective method of microbial decontamination. This study aimed to investigate the bactericidal effect of direct application of an APPJ using helium gas (He-APPJ) on Porphyromonas gingivalis biofilms on sandblasted and acid-etched (SLA) titanium discs. Methods: On the SLA discs covered by P. gingivalis biofilms, an APPJ with helium (He) as a discharge gas was applied at 3 different time intervals (0, 3, and 5 minutes). To evaluate the effect of the plasma itself, the He gas-only group was used as the control group. The bactericidal effect of the He-APPJ was determined by the number of colony-forming units. Bacterial viability was observed by confocal laser scanning microscopy (CLSM), and bacterial morphology was examined by scanning electron microscopy (SEM). Results: As the plasma treatment time increased, the amount of P. gingivalis decreased, and the difference was statistically significant. In the SEM images, compared to the control group, the bacterial biofilm structure on SLA discs treated by the He-APPJ for more than 3 minutes was destroyed. In addition, the CLSM images showed consistent results. Even in sites distant from the area of direct He-APPJ exposure, decontamination effects were observed in both SEM and CLSM images. Conclusions: He-APPJ application was effective in removing P. gingivalis biofilm on SLA titanium discs in an in vitro experiment.

• Journal of Periodontal and Implant Science
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• 제51권5호
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• pp.316-328
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• 2021

Purpose: This study aimed to examine the prevalence and abundance of 9 representative periodontal pathogens in the saliva samples of periodontally healthy subjects (PH) and patients with periodontitis who underwent supportive periodontal therapy (SPT). The age-specific distribution of these pathogens in periodontally healthy individuals was also analyzed. Methods: One hundred subjects (aged >35 years) were recruited (50 each in the PH and SPT groups) between August 2016 and April 2019. The prevalence and abundance of periodontal pathogens in the PH group were compared with those in periodontally healthy young subjects (94 subjects; aged <35 years), who were included in our previous study. DNA copy numbers of Aggregatibacter actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg), Tannerella forsythia (Tf), Treponema denticola (Td), Prevotella intermedia (Pi), Fusobacterium nucleatum (Fn), Campylobacter rectus (Cr), Peptostreptococcus anaerobius (Pa), and Eikenella corrodens (Ec) were analyzed using real-time polymerase chain reaction. Results: The detection frequencies of all pathogens, except Aa, were high in the PH and SPT groups. The ranking order of pathogen DNA copy numbers was similar in both groups. In both groups, Fn had the highest abundance, Aa had the lowest abundance. Additionally, Td was significantly more abundant in men than in women in both groups (P<0.05). Compared with the PH group, the SPT group exhibited significantly lower total bacteria and Fn abundance and higher Pg abundance (P<0.05). The age-specific pathogen distribution analysis revealed a significantly low Aa abundance and high Tf and Cr abundance in the PH group. Conclusions: The clinical parameters and microbial profiles were similar between the SPT and PH groups. However, patients with periodontitis require supportive care to prevent recurrence. As the abundance of some bacteria varied with age, future studies must elucidate the correlation between age-related physiological changes and periodontal bacterial composition.

• 셀메드
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• 제11권1호
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• pp.2.1-2.8
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• 2021

The clinical condition Amnesia causes difficulty in learning new information and the inability to recall past events. It is primarily concerned with recent memory loss. Majoon-e-Nisyan (MJN) is a polyherbal Unani formulation, present in a semi-solid form. It is widely used potent drug of the Unani System of Medicine (USM) for treating Nisyan (amnesia). In the present study polyherbal Unani formulation, MJN has been studied for its quality control and acute toxicity. Standardization (quality control) of drugs deals with drug identity, drug quality and purity determination. Standardization of MJN had been done as per the Unani pharmacopoeial parameters approved by World Health Organization (WHO) - Pharmacognostical parameters, Physico-chemical parameters, high-performance thin-layer chromatography (HPTLC), microbial load, aflatoxin, and heavy metals. Solvents and chemicals used in the study were of analytical grade and used instrument were calibrated. By conducting an acute oral toxicity study in rats, the safety of MJN was assessed. The limit test method of OECD guideline 425 was followed in the study. Results of standardization and standard operating procedures (SOPs) for preparation of MJN may serve as the standard reference in the future. The data generated in the study for the quality control of MJN proved the quality of formulation and shows that MJN is not toxic in rats following acute dosing up to 2000 mg/kg bw. The data obtained in the paper for MJN may be used as a standard guideline for preparation of the formulation which can save time, cost, and resources for future research endeavours.

• 셀메드
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• 제11권4호
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• pp.18.1-18.8
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• 2021

Jawarishe Jalinoos (JJ) is an orally used formulation available in semisolid dosage form, prepared with powdered plant materials mixed in honey or sugar syrup. It has many admirable pharmacological effects and used in Unani medicine to treat various acute and chronic disorders since ancient times. The ICH Harmonised Tripartite Guideline stated that photostability testing should be an essential part of stability testing to confirm that light exposure does not result in an unacceptable change in drugs substance and finished products. To date, the effect of light on JJ is not studied, in this study photostability evaluation of JJ was carried out. The test sample was manufactured with genuine ingredients in the in-door pharmacy of the National Institute of Unani Medicine. JJ was packed in two transparent polyethylene terephthalate airtight containers. The first sample was analysed at zero-day and the second sample was placed in a stability chamber subjected to light challenge with an overall illumination of 1.2 million lux hours combined with near ultraviolet energy of 200-watt hours per square meter by using option 2, along with 30±2℃ temperature and relative humidity 70±5%. Analysis of both finished products showed no considerable changes in organoleptic characters. Less than 5% variation was observed in physicochemical parameters. HPTLC fingerprinting showed justifiable variation. Microbial load and specific counts were within the limit prescribed by WHO. As no unacceptable changes were noted in JJ subjecting to light challenge, it is concluded that JJ is a photostable Unani compound formulation.

• Safety and Health at Work
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• 제13권1호
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• pp.9-16
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• 2022

Background: The global shift toward greener societies demands new technologies and work operations in the waste-management sector. However, progressive industrial methods do not necessarily consider workers' health. This study characterized workers' exposure to bioaerosols and investigated the bioaerosols' potential to engage the immune system in vitro. Methods: Full shift personal aerosol sampling was conducted over three consecutive days. Dust load was analyzed by gravimetry, fungal and actinobacterial spores were analyzed by scanning electron microscopy, and endotoxin by limulus amebocyte lysate (LAL) assay. In vitro exposure of HEK cells to airborne dust samples was used to investigate the potential of inducing an inflammatory reaction. Results: The total dust exposure level exceeded the recommended occupational exposure limit (OEL) of 5.0 mg/m³ in 3 out of 15 samples. The inhalable endotoxin level exceeded the recommended exposure level by a 7-fold, whereas the fungal spore level exceeded the recommended exposure level by an 11-fold. Actinobacterial spores were identified in 8 out of 14 samples. In vitro experiments revealed significant TLR2 activation in 9 out of 14 samples vs. significant TLR4 activation in all samples. Conclusion: The present study showed that the dust samples contained potentially health-impairing endotoxin, fungi, and actinobacterial levels. Furthermore, the sampled dust contained microbial components capable of inducing TLR activation and thus have the potential to evoke an inflammatory response in exposed individuals.

• The Journal of Advanced Prosthodontics
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• 제14권5호
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• pp.273-284
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• 2022

PURPOSE. Computer-aided design and manufacturing (CAD-CAM) of implant abutments has been shown to result in surface contamination from site-specific milling and fabrication processes. If not removed, these contaminants can have a potentially adverse effect and may trigger inflammatory responses of the peri-implant tissues. The aim of the present study was to evaluate the bacterial disinfection and cleaning efficacy of ultrasonic reprocessing in approved disinfectants to reduce the microbial load of CAD-CAM abutments. MATERIALS AND METHODS. Four different types of custom implant abutments (total N = 32) with eight specimens in each test group (type I to IV) were CAD-CAM manufactured. In two separate contamination experiments, specimens were contaminated with heparinized sheep blood alone and with heparinized sheep blood and the test bacterium Enterococcus faecium. Abutments in the test group were processed according to a three-stage ultrasonic protocol and assessed qualitatively and quantitatively by determination of residual protein. Ultrasonicated specimens contaminated with sheep blood and E. faecium were additionally eluted and the dilutions were incubated on agar plates for seven days. The determined bacterial counts were expressed as colony-forming units (CFU). RESULTS. Ultrasonic reprocessing resulted in a substantial decrease in residual bacterial protein to less than 80 ㎍ and a reduction in microbiota of more than 7 log levels of CFU for all abutment types, exceeding the effect required for disinfection. CONCLUSION. A three-stage ultrasonic cleaning and disinfection protocol results in effective bacterial decontamination. The procedure is reproducible and complies with the standardized reprocessing and disinfection specifications for one- or two-piece CAD-CAM implant abutments.

• 한국식품위생안전성학회지
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• 제8권3호
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• pp.135-140
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• 1993

전통조리식품에 널리 이용되어 왔고 현재에도 한국인의 식생활에 있어서 중요한 건어물로 알려진 건명태, 건오징어, 뱅어포, 건멸치와 건대구에 대한 저장성 향상을 위하여 각 시료를 접합 polyethlene 주머니로 함기 포장하고 그동안 많은 연구 결과 공인된 적정선량인 7kGy의 감마선을 조사한 뒤 비조사 시료와 함께 12개월간 저장하면서 미생물의 증식상태를 실험하였따. 저장초기에 비조사 시료는 g당 전호기성 세균의 오염도가 건명태 $3.9{\times}10^{3}$, 건오징어채 $5.6{\times}10^{5}$, 뱅어포 $1.2{\times}10^{5}$, 건멸치 $1.2{\times}10^{4}$, 건대구가 $1.2{\times}10^{4}$마리가 검출되었으나 7kGy의 방사선 조사로서 멸균되었거나 1~3 log cycle 감소되었고 저장기간 중 증식되지 않았다. 비조사 시료의 곰팡이 오염도는 건명태가 g당 $4.0{\times}10^{2}$, 건오징어채 $1.3{\times}10^{5}$, 뱅어포 $2.5{\times}10^{5}$, 건멸치 90, 건대구가 $1.2{\times}10^{2}$마리였으며 방사선 조사시료는 전부 멸균되었고, 비조사 시료의 저장기간 중 생육도는 모든 시료에서 감소하는 경향이었다. 비조사 시료에 있어서 효모 오염도는 건명태가 g당 $1.4{\times}10^{3}$, 건오징어채 75, 뱅어포 $1.1{\times}10^{3}$, 건멸치 50, 건대구가 $1.6{\times}10^{2}$마리였으며 7kGy 조사로 모든 시료의 효모는 멸균되었다. 저장기간 중 효모의 증식상태는 건명태와 뱅어포는 감소하는 경향이었으나 건오징어채, 건멸치, 건대구에서는 증가하였으며 특히 건오징어채에서는 현저하게 증식하였다. 방사선 조사로서 모든 시료의 대장균군은 저장기간중 건 멸치는 현저하게 증식되었으나 기타 시료는 저장 중 감소하는 경향이므로 대중균군의 오염도는 크게 문제가 되지 않는 것으로 생각된다.

• 한국식품과학회지
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• 제47권1호
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• pp.13-19
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• 2015

원산지별 건고추(중국산, 베트남, 국산)의 전자빔 조사처리에 따른 품질변화를 알아보고자 미생물학적 및 이화학적 품질 특성을 확인하였다. 비조사처리 건고추의 미생물 오염농도는 총 세균, 효모 및 곰팡이가 $10^6-10^7CFU/g$이 확인되었고, 대장균군은 베트남산에서만 $10^3CFU/g$이 확인되었다. 그러나 전자빔 조사처리에 의해 총 세균, 효모 및 곰팡이는 약 2-4 log cycle 정도 감소되었으며, 대장균군은 1 kGy 이상에서 검출되지 않았다. 또한 원산지에 따른 전자빔 조사처리 된 건고추의 수분, pH는 조사선량에 따른 유의적인 차이가 나타나지 않았으며, 환원당 함량은 1 kGy에서 다소 증가하는 경향이 있으나 유의적인 차이는 미미한 것으로 나타났다. 고추의 주요 매운맛 성분인 capsaicinoid함량은 중 국산(19.21 mg%), 베트남산(214.36 mg%), 국내산(7.28 mg%)으로 베트남산이 가장 높게 나타났으며, 조사선량이 증가할수록 그 함량은 감소하였다. 그러나 전자빔 조사처리 고추의 L 값 및 a 값은 유의적인 차이가 나타나지 않았으나, b 값은 조사선량이 증가할수록 증가하였으며, capsanthin 함량 및 ASTA values는 선량에 의한 영향보다 원산지에 의해 더 큰 영향을 받는 것으로 나타났다. 따라서 본 연구에서는 미생물을 효과적으로 살균하고 품질변화를 최소화하는 고추의 위생적 살균방법으로 전자빔 살균기술의 타당성을 확인하였다.

• 한국식품과학회지
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• 제49권2호
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• pp.117-122
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• 2017

신선 채소인 파프리카에 검역처리 선량의 전자선 처리(0, 0.4, 1, 2 kGy) 후 냉장저장 40일 동안 미생물학적, 이화학적 및 관능적 품질을 평가하였다. 파프리카의 미생물 농도는 총세균, 효모 및 곰팡이의 경우 $10^4CFU/g$, 대장균군은 $10^2CFU/g$ 수준이었고, 1 kGy 선량은 미생물 농도를 1 log cycle 감소시킬 수 있었다. 중량감소율은 조사처리에 따른 차이를 보이지 않았고, 경도와 카로티노이드 함량은 조사 직후 1 kGy 이상의 선량에서 유의적으로 감소하였으며, 저장 중에도 선량에 관계없이 감소하였다. 비타민 C 함량은 선량에 따른 유의적인 차이를 보이지 않았고, 0, 0.4 및 1 kGy 처리 시료는 저장 40일째 저장 초기 함량의 87-90% 수준으로 감소하였다. 파프리카에 대한 관능평가 결과, 2 kGy 수준의 전자선 처리는 색과 조직감 저하에 영향을 미치는 것으로 확인되었다. 이상의 결과, 수출용 파프리카의 해충구제 및 검역처리를 위한 0.4-1 kGy 수준의 전자선 처리는 냉장저장 동안 미생물학적 안전성을 확보하면서 이화학적 품질을 유지시키는 것으로 확인되었다.