• 한국축산식품학회지
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• 제33권2호
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• pp.205-213
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• 2013

The objective of this study was to evaluate the quality characteristics of sun-dried Hanwoo beef jerky added with Salicornia herbacea (SH). Sliced Hanwoo beef shank were marinated and sun-dried at $28-30^{\circ}C$, relative humidity (RH) 30-35% for 3.5 h. The physicochemical and microbiological traits of the Hanwoo beef jerky were analyzed during the aerobically packaged storage at $25^{\circ}C$. The water activities of beef jerky with 0.5% and 1.0% SH were lower than those of the control at 0 d (p<0.05). The pH values of beef jerky with SH were significantly higher than those of the control (p<0.05). The beef jerky with SH and ascorbic acid showed significantly lower TBARS values than the control (p<0.05). The beef jerky with SH showed a significantly lower redness ($a^*$) than the control (p<0.05). Total plate count (TPC) of beef jerky with 1.0% SH was significantly lower than that of the control during the storage of 20 d (p<0.05). Yeast/mold was detected in the control and beef jerky with SH after storage periods of 10 and 20 d, but was not detected in jerky with ascorbic acid. With regard to the sensory properties, beef jerky with SH showed significantly greater flavor scores than the others (p<0.05). The result shows that SH powder can be used to increase the sensory quality and microbial safety of beef jerky.

• 한국식품위생안전성학회지
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• 제27권4호
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• pp.415-419
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• 2012

선식용 원료로 사용되는 검정쌀, 검정콩, 차조를 원료로 사용하여 열풍건조에 따른 물리화학적 효과와 미생물학적 효과를 조사하였다. 일반성분의 경우 대조구에 비해 수분함량은 8%정도로 건조하였으며 이에 따라 단백질, 탄수함량, 지질, 회분의 성분이 대조구에 비해 증가하는 경향을 나타내고 있었으나 건조온도에 따라서는 유의차가 없는 범위에서 차이가 없었다. 색차의 경우 L값, a값, b값이 증가하였으나 흑미의 경우 b값이 변화가 없었으며, 차조는 L값이 차이가 없었다. 건조조건에 선식원료의 물성을 측정한 결과, 대조구에 비해 건조 선식용 시료는 물성이 감소하였으며 건조온도가 높을수록 물성치는 증가하였다. 건조조건에 따른 선식용 원료의 미생물 변화를 살펴본 결과 총균수는 2 log 내지 3 log 감소하였으며 B. cereus를 제외하고는 다른 병원성 미생물은 검출되지 않았다.

• 방사선산업학회지
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• 제5권3호
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• pp.267-272
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• 2011

This study evaluated the microbial population (total aerobic count, fungi) and organoleptic quality of freeze-dried bibimbap sterilized by gamma irradiation to develop the space food. An irradiation dose above 30 kGy was needed to sterilize the freeze-dried bibimbap, while organoleptic quality of the sample was significantly decreased by irradiation (p<0.05). However, it was observed that scores on overall acceptance of rehydrated bibimbap after irradiation were the highest, when vitamin C and paprika extract were added at 0.1%, respectively. Therefore, it was considered that the freeze-dried bibimbap could be developed as a space food, which meet microbial requirements and organoleptic quality through addition of vitamin C 0.1% and paprika extract 0.1% before gamma irradiation at 30 kGy.

• 대한영양사협회학술지
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• 제25권1호
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• pp.1-16
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• 2019

This study examined the microbiological quality of daycare center meals. Six menu items from five daycare centers in Daegu included uncooked processed foods (seasoned cucumber and lettuce salad), post-preparation after cooking processed foods (rolled omelet and seasoned soybean sprout), and cooking processed foods (panbroiled beef with oyster sauce and seasoned pork roast). Microbiological analyses were performed for the aerobic plate counts (APC), coliforms, and Escherichia coli. The analyses were conducted in July and November 2017. The mean APC and coliform count of seasoned cucumber decreased significantly from 4.71 log colony forming units (CFU)/g and 2.50 log CFU/g in July to 4.07 log CFU/g and 1.78 log CFU/g in November, respectively (P<0.01 and P<0.001). The APC of panbroiled beef with oyster sauce and seasoned pork roast were significantly lower in July (1.84 and 1.79 log CFU/g) than in November (2.41 and 2.28 log CFU/g) (P<0.001). The coliform counts of panbroiled beef with oyster sauce and seasoned pork roast were significantly greater in November (2.11 and 1.62 log CFU/g) (P<0.001). E. coli was not detected. Among the foods prepared using the three preparation processes, the post-preparation after cooking processed foods had the lowest microbial quality. The APC and coliform counts of cooking processed foods were satisfactory in July, with an acceptable rating for pan-broiled beef with oyster sauce in November. Time-temperature control and the prevention of cross-contamination are essential during meal production for food safety, regardless of the season.

• 한국축산식품학회지
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• 제39권2호
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• pp.266-275
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• 2019

Although the production of dry-aged beef has been increasing, most purveyors are unaware of the changes in quality that ensue after completion of the aging period and do not adhere to specific guidelines for its packaging and storage. The objective of this study was to investigate the storage stability of vacuum-packaged dry-aged beef based on changes in microbial, physicochemical, and sensory properties during refrigeration at $4^{\circ}C$ for 21 d. The total aerobic bacterial count exceeded 6 Log CFU/g at approximately day 11 and significantly increased after day 14. Freshness indicators such as pH and volatile basic nitrogen content were acceptable until day 14 and 21, respectively. Based on the evaluation of overall sensory acceptability, the dry-aged beef was acceptable until 14 d without any sensory deterioration. Therefore, vacuum-packaged dry-aged beef could be stored for 11 d at $4^{\circ}C$ without any adverse effect on its microbial and sensory quality.

• Journal of Veterinary Science
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• 제24권2호
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• pp.20.1-20.13
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• 2023

Background: As Actinobacillus pleuropneumonniae (APP) infection causes considerable losses in the pig industry, there is a growing need to develop effective therapeutic interventions that leverage host immune defense mechanisms to combat these pathogens. Objectives: To demonstrate the role of microRNA (miR)-127 in controlling bacterial infection against APP. Moreover, to investigate a signaling pathway in macrophages that controls the production of anti-microbial peptides. Methods: Firstly, we evaluated the effect of miR-127 on APP-infected pigs by cell count/enzyme-linked immunosorbent assay (ELISA). Then the impact of miR-127 on immune cells was detected. The cytokines tumor necrosis factor (TNF)-α and interleukin (IL)-6 were evaluated by ELISA. The expression of cytokines (anti-microbial peptides [AMPs]) was assessed using quantitative polymerase chain reaction. The expression level of IL-6, TNF-α and p-P65 were analyzed by western blot. The expression of p65 in the immune cells was investigated by immunofluorescence. Results: miR-127 showed a protective effect on APP-infected macrophage. Moreover, the protective effect might depend on its regulation of macrophage bactericidal activity and the generation of IL-22, IL-17 and AMPs by targeting sphingosine-1-phosphate receptor3 (SIPR3), the element involved in the Toll-like receptor (TLR) cascades. Conclusions: Together, we identify that miR-127 is a regulator of S1PR3 and then regulates TLR/nuclear factor-κB signaling in macrophages with anti-bacterial acticity, and it might be a potential target for treating inflammatory diseases caused by APP.

• Journal of Animal Science and Technology
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• 제66권1호
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• pp.178-203
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• 2024

Constipation, which refers to difficulties in defecation and infrequent bowel movement in emptying the gastrointestinal system that ultimately produces hardened fecal matters, is a health concern in livestock and aging animals. The present study aimed to evaluate the potential effects of dairy-isolated lactic acid bacteria (LAB) strains to alleviate constipation as an alternative therapeutic intervention for constipation treatment in the aging model. Rats were aged via daily subcutaneous injection of D-galactose (600 mg/body weight [kg]), prior to induction of constipation via oral administration of loperamide hydrochloride (5 mg/body weight [kg]). LAB strains (L. fermentum USM 4189 or L. plantarum USM 4187) were administered daily via oral gavage (1 × 10 Log CFU/day) while the control group received sterile saline. Aged rats as shown with shorter telomere lengths exhibited increased fecal bulk and soften fecal upon administration of LAB strains amid constipation as observed using the Bristol Stool Chart, accompanied by a higher fecal moisture content as compared to the control (p < 0.05). Fecal water-soluble metabolite profiles showed a reduced concentration of threonine upon administration of LAB strains compared to the control (p < 0.05). Histopathological analysis also showed that the administration of LAB strains contributed to a higher colonic goblet cell count as compared to the control (p < 0.05). The present study illustrates the potential of dairy-sourced LAB strains as probiotics to ameliorate the adverse effect of constipation amid aging, and as a potential dietary intervention strategy for dairy foods including yogurt and cheese.

• 대한환경공학회지
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• 제27권12호
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• pp.1311-1320
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• 2005

본 연구에서는 pilot 규모의 활성탄 공정을 운전하면서 입상활성탄(granular activated carbon: GAC) 단계에서부터 생물활성탄(biological activated carbon: BAC) 단계로 전환되고 난 후 까지 활성탄 재질별로 유기물 제거능과 미생물 군집특성을 함께 조사하였다. 활성탄 재질별 유기물 흡착능은 석탄계 재질의 활성탄이 가장 우수하였고, bed volume 20,000 이후부터는 3가지 활성탄들이 정성상태에 도달하였다. 부착세균의 생체량과 생산력 또한 석탄계 재질 활성탄에서 가장 높은 것으로 나타났으며, heterotrophic plate count(HPC), eubacteria(EUB), 4,6-diamidino-2-phenylindole(DAPI) 및 생산력은 각각 $0.95{\times}10^7{\sim}52.4{\times}10^7$ CFU/g, $3.8{\times}10^8{\sim}134.2{\times}10^8$ cell/g, $7.0{\times}10^8{\sim}250.2{\times}10^8$ cell/g 및 $1.2{\sim}3.4\;mg{\cdot}C/m^3{\cdot}h$의 범위로 나타났다. 그리고 부착세균의 생체량과 생산력은 모두 bed volume 20,000 이후부터 증가하는 경향을 보였다. 활성탄 재질별 부착세균 생체량과 세균 생산력에 대한 동화가능한 유기탄소(assimilable organic carbon: AOC) 제거율과의 상관성 평가에서는 석탄계 재질 활성탄이 가장 양호한 상관성을 보였으며, 항목별로는 세균 생산력에 대한 상관성이 상대적으로 높은 것으로 나타났다. Fluorescent in situ hybridization(FISH)에 의한 세균군집 구조 조사결과, bed volume 20,000까지는 모든 활성탄에서 $\alpha$ 그룹($\alpha$-proteobacteria)과 other bacteria가 우점하였고, bed volume 20,000 이상에서는 석탄계 재질 환성탄에서는 $\beta$ 그룹($\beta$-proteobacteria)과 $\gamma$ 그룹($\gamma$-proteobacteria)의 우점비율이 상승하였으나, 야자계와 목탄계에서는 $\alpha,\;\beta$ 및 $\gamma$ 그룹의 우점비율이 상승하는 것으로 조사되었다.

• 한국식품저장유통학회지
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• 제14권1호
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• pp.1-7
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• 2007

농산물의 미생물학적 안전성에 대한 요구가 증대되면서 미생물에 의한 농산물의 오염 및 균의 발생을 최소화하기 위해 이산화염소($ClO_{2}$) 가스를 이용한 포도저장 실험을 수행하였다. 실험 결과 중량감소율은 상온, 저온저장 모두 20 ppm+Ny/PE/L-LDPE 처리구가 가장 적었고 가용성 고형물 함량은 상온, 저온저장 모두 무처리구가 다른 포장구에 비해 높은 값을 나타내었으며 20 ppm+Ny/PE/L-LDPE 처리구는 감소했다. 적정산도 값은 상온, 저온저장 중 모든 포장구에서 감소하는 경향을 나타내었으며 Anthocyanin 함량은 상온저장에서는 모든 포장구에서 저장 14일까지 증가하다가 저장 21일에 급격히 감소했으며 저온저장에서는 저장 10주까지 모든 포장구간이 초기보다 증가하는 경향을 나타내었다. 총균수 값은 상온저장 시 $ClO_{2}$ gas처리 및 silica gel pad 처리구가 총균수 억제효과를 나타내었고 저온저장에서는 $ClO_{2}$ gas를 처리한 20 ppm+Ny/PE/L-LDPE와 40 ppm포장구에서 총균수 억제효과를 나타내어 저온보다 상온에서 $ClO_{2}$ gas의 효과가 더 크게 작용하는 것으로 나타났다.

• 한국가금학회지
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• 제47권3호
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• pp.169-180
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• 2020

본 연구는 복합생균제(L. plantarum, B. subtilis, Saccharomyces cerevisiae)를 0%(CON, 대조군), 0.25%(PC1) 및 0.5%(PC2) 수준으로 급여하여 육계의 생산성, 장기 무게, 혈액 생화학적 성상 및 면역지표, 소화효소 활성도, 분의 미생물 군락 및 유해가스 발생에 미치는 영향을 조사하기 위해 실시되었다. 복합생균제 급여는 체중 등과 같은 생산성에는 유의적 영향을 미치지 않았다. 간과 흉선 무게는 복합생균제 급여에 따른 영향이 없었으나, 소장 점막세포 무게는 PC1군에서 유의하게(P<0.05) 증가하였다. Glucose, cholesterol, AST, ALT 등과 같은 혈액 생화학성분은 복합생균제 급여에 따른 변화가 없었다. 분비형 면역글로불린 A(sIgA) 수준은 PC2군에서 대조군과 비교해 소장 점막세포에서 유의하게(P<0.05) 증가하였으며, 혈액에서도 PC2군에서 대조군보다 약 20% 증가하는 경향을 보였다. 혈액과 소장 점막세포의 IL-1β 수준은 복합생균제 급여에 따른 차이가 없었다. 또한, 복합생균제 급여가 소장 점막세포의 maltase, sucrase 및 leucine aminopeptidase 활성도에는 영향을 미치지 않았다. 한편 Lactobacillus 및 Saccharomyces cerevisiae cfu 수준은 복합생균제 0.5% 급여군에서 대조군보다 유의하게(P<0.05) 증가하였고, E. coli cfu 값은 감소하였다(P<0.05). 복합생균제 0.5% 급여 시 분에서 황화수소(H2S) 발생량은 유의하게(P<0.05) 감소하였으며, 메틸메르캅탄(CH3SH) 발생량 역시 50% 수준으로 낮았다. 결론적으로 복합생균제 급여(0.25% 및 0.5%)는 육계의 생산성에는 영향을 미치지 않았지만 0.5% 수준으로 급여할 경우 소장 점막세포의 sIgA 증가와 유익 미생물 균총의 증식을 유도하여 분의 유해가스 발생을 감소시키는 것으로 나타났다.