• 한국식품과학회지
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• 제21권3호
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• pp.379-386
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• 1989

본 연구에서는 농축대두단백으로 두유를 만들고 미생물 pretense와 papain을 단독 또는 혼합 사용하여 대두단백질을 가수분해한 후, 단백질이 일부 가수분해 된 두유에서 L. acidophilus의 생육과 산생성을 관찰하고 제조된 젖산균음료의 관능성을 조사하였다. Pretense 처리로 젖산균의 산생성이 촉진되었는데 미생물 pretense가 papain보다 효과적이었으며 2종의 효소를 혼합 사용했을 때는 상승효과가 보였다. 그러나 pretense 처리로 pH와 생균수는 큰 변화가 없었다. 미생물 pretense의 경우는 가수분해시간 15분까지, papain의 경우는 가수분해시간 45분까지 산생성 촉진효과가 현저했으나 그 후에는 3시간이 경과하여도 큰 변화가 없었다. 미생물 pretense 0.2% 또는 papain 0.2% 처리로 대두젖산균음료의 전체적인 기호도와 맛이 다소 개선되었다. Protease 처리 15분에 비단백태질소의 함량이 현저하게 증가하였고 후 3시간까지 서서히 증가하였다.

• 한국미생물·생명공학회지
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• 제42권4호
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• pp.377-385
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• 2014

본 연구에서는 고염폐수의 정화능이 우수한 미생물 기능성 커뮤니티 HWTC (Highsalt Wastewater Treatment Community)를 이용한 고염폐수 처리시스템을 개발하였으며, HWTC의 미생물 군집의 다양성을 확인해 보았다. HWTC의 고염폐수 처리능력은 HRT 2.5일만에 $COD_{cr}$ 84%의 처리효율로 확인하였다. 미생물 군집분석은 PCR-DGGE 기법과 16S rRNA gene clone library를 통하여 미생물 다양성을 확인하였다. 4%의 염농도의 폐수에서 우점하는 미생물은 Halomonas sp.와 Paenibacillus sp.로 나타났고, phylogenetic tree 분석을 통해 ${\gamma}$-proteobacteria 속하는 미생물의 다양성이 높게 나타났으며, firmicutes속 하는 미생물이 우점하고 있었다. 고염폐수를 처리할 수 있는 미생물 기능성 커뮤니티 HWTC를 이용하여, 고염의 폐수 정화를 가능하게 할 것으로 판단된다.

• 한국환경보건학회지
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• 제46권2호
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• pp.170-183
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• 2020

Objectives: This study was conducted to estimate a technology to reduce hydrogen sulfide (H₂S) in sewage odor using microbial deodorant. Methods: After injecting five commercially available microbial deodorants into fresh sewage, the concentration of hydrogen sulfide over time was measured using the headspace method. H₂S concentration in odor samples was measured using gas chromatograph/FPD. Calculated odor concentration and calculated odor intensity by H₂S concentration remaining after treatment with microbial deodorant were evaluated theoretically. Results: The rate of H₂S abatement by microbial deodorant differed depending on the experimental conditions and the type of deodorant, but it was found to range from 63 to 82%. Especially, two deodorants showed high H₂S reduction rates of over 80% on average. However, based on the best deodorant, the theoretically calculated odor concentration by H₂S after microbial deodorant treatment was 4,400 OU_k, and the theoretical odor intensity was also rated at 4 degrees or higher. Conclusions: In conclusion, microbial deodorant is considered to have a relatively high effect on reducing H₂S in sewage odor. However, even after treatment with microbial deodorant, calculated odor concentration and calculated odor intensity were relatively high. This is thought to be caused by other odorous substances besides H₂S.

• Environmental Engineering Research
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• 제22권2호
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• pp.157-161
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• 2017

Simultaneous treatment of food waste leachate and power generation was investigated in an air-cathode microbial fuel cell. A TCOD removal efficiency of $95.4{\pm}0.3%$ was achieved for an initial COD concentration of 2,860 mg/L. Maximum power density ranged was maximized at $1.86W/m^3$, when COD concentration varied between 60 mg/L and 2,860 mg/L. Meanwhile, columbic efficiency was determined between 1.76% and 11.07% for different COD concentrations. Cyclic voltammetric data revealed that the oxidation peak voltage occurred at -0.20 V, shifted to about -0.25 V. Moreover, a reduction peak voltage at -0.45 V appeared when organic matters were exhausted, indicating that reducible matters were produced during the decomposition of organic matters. The results showed that it was feasible to use food waste leachate as a fuel for power generation in a microbial fuel cell, and the treatment efficiency of the wastewater was satisfied.

• 상하수도학회지
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• 제20권3호
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• pp.461-467
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• 2006

The seasonal change in attached algae and microbial community structure at sedimentation basin of water treatment plant was investigated by using quinone profiles and denaturing gel gradient electrophoresis (DGGE). The photosynthetic bacteria and algae contains PQ-9 and VK-1 as major quinone are major component of the total quinone fraction in attached algae and microorganisms on sedimentation basin trough. The microorganisms containing menaquinones appear to be sensitivity to the change in temperature than those containing ubiquinones. The plot of the mole fraction of dominant quinone species ($f_d$) to the DQ values showed higher sensitivity to the seasonal change in the microbial community structure. The results indicated that quinone and DGGE are useful tool for the evaluation of the changes in the microbial community structure.

• 한국유기농업학회지
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• 제12권4호
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• pp.399-409
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• 2004

This study was conducted to investigate the effect of different concentrations of microbial products on growth of chinese cabbage and microorganisms in soil. Two different levels of microbial products, such as 50 times and 100 times diluted solutions of chitosan, wood vinegar and EM activity liquid, were treated for foliar application. the results were summarized as follows : Among foliar applications of microbial products, 100 times diluted solution of chitosan was effective on growth of chinese cabbage comparing to other levels of dilutions and untreated control plot. The number of microorganism in the soil tended to increase under the treatment of microbial products compared to control plot. Especially, the numbers of the bacteria and actinomycetes were estimated $73.67{\times}10^3$ CFU/g and $34.00{\times}10^3$ CFU/g, respectively, under the treatment of 100 times diluted solution of chitosan.

• Environmental Engineering Research
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• 제14권2호
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• pp.120-125
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• 2009

Water stress has become a major concern in agriculture. Korea suffers from limited agricultural water supply, and wastewater reuse has been recommended as an alternative solution.A study was performed to examine the effects of microorganism concentration in the ponded-water of a paddy rice field with reclaimed-water irrigation for evaluating the microbial risk to farmers and neighborhoodchildren.Most epidemiological studies were performed based on an upland field, and they may not directly applicable to paddy fields. Beta-Poisson model was used to estimate the microbial risk of pathogen ingestion. Their risk value increased significantly high level after irrigation and precipitation.It implies that agricultural activities such as plowing, and fertilizing, and precipitation need be practiced a few days after irrigation considering health risks. The results about field application of the microbial risk assessment using E. coli showed difference according to monitoring time and treatment plot. Result of the microbial risk assessment showed that risk values of ground-water and reclaimed secondary waste water irrigation were lower than directly use of wastewater treatment plants' effluent. This paper should be viewed as a first step in the application of quantitative microbial risk assessment of E. coli to wastewater reuse in a paddy rice farming.

• Journal of Applied Biological Chemistry
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• 제48권4호
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• pp.187-192
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• 2005

Influence of herbicide oxadiazon on soil microbial activity and nitrogen dynamics was evaluated. Soil samples were treated with oxadiazon at field and tenfold field rates and incubated. Organic amendment was added as an additional substrate for soil microorganisms. Tenfold field rate oxadiazon stimulated substrate-induced respiration (SIR) and dehydrogenase activity (DHA) in amended soil as compared to unamended soil and control treatment. Soil urease activity was not affected by oxadiazon treatment. In both amended and unamended soils, treatment of the herbicide at higher rate had not significant influence on $NH_4$-N and $NO_3$-N concentrations. Higher dose of oxadiazon was degraded in both soils, but dissipation rate in amended soil was higher than unamended soil, with half-lives ($t_{1/2}$) of 23.1 and 138.6 days, respectively. Recommended field rate did not affect microbial activity and nitrogen dynamics in soil ecosystem. Results showed influence of oxadiazon on cycling processes of nitrogen in soil was not significant however its effect on microbial activity was a tendency depending on addition of organic amendment to soil.

• 한국식품위생안전성학회지
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• 제27권4호
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• pp.442-448
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• 2012

고춧가루의 LED(적색, 황색, 청색, 녹색광) 처리시 저장기간 중 미생물 저감화, 수용성 색소, capsanthin 함량, 표면 색도(Hunter L, a, b, ${\Delta}E$) 및 관능평가를 실시하였다. 저장기간 중 총 호기성 세균은 유의적인 차이를 나타내지 않았으며, 효모 및 곰팡이의 경우 황색 LED 처리군에서 1.76 log 감소되었다. 표면 색도는 저장기간이 경과함에 따라 모든 시료군에서 명도(L), 적색도(a) 및 황색도(b)가 유의적으로 감소하는 경향을 나타내었다. 전반적인 색차는 황색 LED 시료에서 ${\Delta}E$ 값이 3.0 이하로 나타나 다른 시료군에 비해 색도의 변화가 적은 것으로 나타났다. 수용성 색소는 저장기간 중 유의적인 차이는 나타나지 않았으며, capsanthin 함량은 저장기간이 경과함에 따라 감소하였으나 적색 LED 시료와 황색 LED 시료는 저장 10일 후 초기와 유의적인 capsanthin 함량 차이를 나타내지 않았다. 관능적 품질은 대조군의 경우 외관, 색 및 상품성이 저장기간 중 유의적으로 감소하는 경향을 나타내었으나 황색 LED 시료와 녹색 LED 시료는 유의적인 차이가 나타나지 않았다.

• 한국축산식품학회지
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• 제44권2호
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• pp.309-325
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• 2024

The consumption of meat has been increasing, leading to a dynamic meat and meat processing industry. To maintain the quality and safety of meat products, various technologies have been explored, including intense pulsed light (IPL) technology. Several factors affect the inactivation of microorganisms by IPL treatment, including light intensity (fluence), treatment duration, pulse frequency, and the distance between the lamp and the samples. Meat products have been studied for IPL treatment, resulting in microbial reductions of approximately 0.4-2.4 Log. There are also impacts on color, sensory attributes, and physico-chemical quality, depending on treatment conditions. Processed meat products like sausages and ham have shown microbial reductions of around 0.1-4 Log with IPL treatment. IPL treatment has minimal impact on color and lipid oxidation in these products. Egg products and dairy items can also benefit from IPL treatment, achieving microbial reductions of around 1-7.8 Log. The effect on product quality varies depending on the treatment conditions. IPL technology has shown promise in enhancing the safety and quality of various food products, including meat, processed meat, egg products, and dairy items. However, the research results on animal-based food are not diverse and fragmentary, this study discusses the future research direction and industrial application through a review of these researches.