• 한국패류학회지
• /
• 제31권1호
• /
• pp.35-41
• /
• 2015

실험에 사용된 미세조류는 이매패류 종묘생산시 유생과 치패의 먹이생물로 주로 이용되고 있는 Isochrysis galbana, Pavlova lutheri, Chaetoceros simplex, Tetraselmis tetrathele 등 4종을 선정하였으며, 배양환경으로 수온 (20, 25, 30, $35^{\circ}C$), 염분 (20, 25, 30, 33 psu) 그리고 조도 (60, 100, $140{\mu}mol\;m^{-2}s^{-1}$) 를 서로 달리하여 4종의 미세조류에 대한 성장을 조사하였다. I. galbana의 성장은 수온 $20^{\circ}C$ 보다 $25^{\circ}C$에서 빠른 성장을 나타내었으며, 수온 $25^{\circ}C$일 때 일간 성장률은 염분 33 psu에서 0.413으로 가장 높았고, 20 psu에서 0.368로 가장 낮게 나타났다 (P < 0.05). 25 psu에서 일간 성장률은 0.383으로 30 psu 보다 낮게 나타났고, 20 psu 보다 높은 경향을 보였다 (P < 0.05). 이와 같은 수온과 염분에 따른 미세조류의 성장률은 종별로 다소 차이는 있으나 P. lutheri, T. tetrathele에서도 I. galbana의 성장과 유사한 경향을 나타내었다. 한편, C. simplex의 성장은 수온 $25^{\circ}C$ 보다 $30^{\circ}C$에서 빠른 성장을 나타내었으며, 수온 $30^{\circ}C$에서 일간 성장률은 배양 10일째 염분 33 psu에서 0.428로 가장 높았고, 20 psu에서 0.389로 가장 낮게 나타났다 (P < 0.05). 그리고 서로 다른 조도에서 배양한 미세조류 4종의 성장은 $100{\mu}mol\;m^{-2}s^{-1}$ 보다 $140{\mu}mol\;m^{-2}s^{-1}$의 조도에서 가장 빠른 성장을 나타내었다 (P < 0.05).

• Fisheries and Aquatic Sciences
• /
• 제14권4호
• /
• pp.339-345
• /
• 2011

Although culture techniques for the abalone Haliotis discus hannai are well known, mass culture of the benthic microalgae that are essential live food for the abalone larvae is still not practiced. This study was conducted to identify the microalgal species suitable for the growth of early larvae of H. discus hannai. The growth and attachment rates of 31 microalgal species were examined. Acrylic plates were used as the substrate. Among the 31 microalgal species, nine showing high growth and attachment rates were selected and tested for their dietary values via factors including settlement, metamorphosis, and survival rates of abalone larvae. Tetraselmis hazeni and Rhaphoneis sp. induced the highest settlement rate (65-69%) in abalone larvae. The metamorphosis rate was highest (57%) in larvae fed Rhaphoneis sp. and was also significantly higher in larvae fed Oscillatoria splendida (29%) and T. hazeni (22%) than in those fed other species. The highest survival rate of the larvae during the 15 days after metamorphosis was 67% in those fed Rhaphoneis sp., followed by T. hazeni (42%) and O. splendida (35%). In conclusion, Rhaphoneis sp. is the most suitable diatom for use as a live food for the culture of early larvae of H. discus hannai. In addition, T. hazeni and O. splendida are also potential species to be further developed and utilized in larval culture.

• Korean Chemical Engineering Research
• /
• 제51권1호
• /
• pp.87-92
• /
• 2013

미세조류는 바이오연료를 생산하기 위해 필요한 성분인 지방질의 생산성이 우수하기 때문에 바이오연료의 유망한 원료로서 최근 많은 주목을 받고 있다. 본 연구에서는, 이러한 미세조류의 성장 속도와 미세조류 내부의 지방의 함량이 최대가 되도록 하기 위한 목적으로, 미세조류의 성장과 지방의 생성을 설명하는 제일원리(first principle)에 근거한 상미분방정식(ODE) 모델에 대하여 조사하였다. 모델은 6개의 상태변수와 12개의 파라미터로 이루어져 있으며, 미세조류의 성장을 영양분의 흡수와 흡수된 영양분에 의한 성장으로 두 단계로 나누어 설명한 Droop 모델의 가정을 따른다. 본 연구에서는 민감도 분석(Sensitivity analysis)을 위한 최대의 정보를 줄 수 있는 입력 신호를 결정하기 위해 D-optimality criterion을 이용한 최적 입력 설계(Optimal input design)를 수행하였으며, 구하여진 입력 신호를 적용하여 민감도 분석을 수행하여 모델에 좀 더 중요한 파라미터를 결정하였다. 또한 미세조류의 성장속도와 지방의 함량이 최대가 되도록 하기 위하여 모델 예측 제어(MPC)를 수행하였다.

• 생명과학회지
• /
• 제7권4호
• /
• pp.377-383
• /
• 1997

The acute toxicity effect of triorganotin of trioganotin on the growth of microalgae and shellfish was investigated through flask culture. The value of 120 hr-LC$_{50}$ that is the median lethal concentration of TBTO on the shellfish (R. philipinarum) was found to be 6 $\mu$g/L. The acute toxicity effect of TBTO on T. suecica was obviously shown even at the concentration of 0.5 $\mu$g/L, and the effect diminished as the initial cell density increased. The effect also diminished less in the experiment done under aeration than in that done under non-aeration. To design a chemostat system for the test of chronic toxicity, the culture of T. suecica was executed in photobioreactor. In batch culture, the profiles of chlorophyII a and D.C.W. showed the growth of T. suecica very well, and the maximum specific growth rate was estimated to be 0.54 d$^{-1}$. with this value, as a dilution rate in contimuous culture, pH was nicely maintained between 7 and 9 when air was supplied with 3% CO$_{2}$. From all results and the natural environment of clam, a novel chemostat system was invented. Through this system, we can observe each independent toxicity effect of TBTO and plankton and combined toxicity effect as well.

• Korean Chemical Engineering Research
• /
• 제53권4호
• /
• pp.468-471
• /
• 2015

본 연구에서는 빛의 조사기간을 변화시켜 세 종류의 미세조류(Nanochloris, Dunaliella tertiolecta, Tetraselmis)를 배양하고 이들의 성장속도 및 지질 함량을 분석하였다. 빛의 조사기간은 한국의 여름철과 겨울철의 일조시간을 반영해 각각 14.5시간과 7시간으로 설정하였다. 또한, 지질 추출 후 남은 미세조류를 당화시켜 포도당 전환율을 비교함으로써 미세조류의 바이오매스로써의 가능성을 가늠하고자 하였다. 실험 결과 D. tertiolecta가 다른 두 종의 미세조류보다 빛의 조사기간이 7시간일 때 최대 38% 높은 성장속도를 나타냈으며 지질함량은 최대 43.6% 정도 높은 결과를 보였다. 포도당으로의 당화 전환율도 D. tertiolecta가 최대 22% 높은 결과를 보였다.

• Environmental Engineering Research
• /
• 제19권3호
• /
• pp.283-287
• /
• 2014

Cultivation of microalgae using wastewater exhibits several advantages such as nutrient removal and the production of high valuable products such as lipid and pigments. With this study, two types of wastewater from instant noodle factory; mixed liquor suspended solids (MLSS) and effluents after sedimentation tank were investigated for green microalga Scenedesmus sp. cultivation under laboratory condition. Optimal wastewater dilution percentage was evaluated in 24 wells microplate. MLSS and effluent without dilution showed the highest specific growth rate (${\mu}$) of $1.63{\pm}0.11day^{-1}$ and $1.57{\pm}0.16day^{-1}$, respectively, in which they were significantly (p < 0.05) higher than Scenedesmus sp. grown in BG11 medium ($1.08{\pm}0.14day^{-1}$). Ten days experiment was also conducted using 2000 ml Duran bottle as culture vessel under continuous light at approximately 5000 lux intensity and continuous aeration. It was found that maximum biomass density of microalgae cultivated in MLSS and effluent were $344.16{\pm}105.60mg/L$ and $512.89{\pm}86.93mg/L$ respectively and there was no significant (p < 0.05) difference on growth to control (BG11 medium). Moreover, cultivation microalgae in wastewater could reduce COD in wastewater by 39.89%-73.37%. Therefore, cultivation of Scenedesmus sp. in wastewater from instant noodle factory can yield microalgae biomass production and wastewater reclamation using photobioreactor simultaneously.

• 한국해양바이오학회지
• /
• 제8권2호
• /
• pp.45-53
• /
• 2016

Most of microalgae shift their metabolic pathways toward the fatty acid biosynthesis following nitrogen deprivation. Recent studies on Nannochloropsis species, oleaginous microalgae, have been performed to investigate the regulation of contents and compositions of fatty acids under stressful condition. The objective of this experiment is to identify the effect of nitrogen on cell growth and fatty acids production in Nannochloropsis oculata K-1281 and compare fatty acid composition response to nitrogen deficiency between N. oculata LB2164 and K-1281. The fatty acids content in N. oculata K-1281 was increased up to 210%, while the growth rate was decreased under nitrogen deficient condition. The contents of C16:0 and C16:1 increased dramatically in both N. oculata K-1281 and LB2164, while the contents of C20:4 and C20:5 increased in N. oculata LB2164. The fatty acids content and composition in N. oculata K-1281 returned following addition of nitrogen after nitrogen starvation. These results demonstrated that fatty acid contents and compositions under nitrogen deficiency will provide the understanding of fatty acid synthesis in microalgae.

• 공업화학
• /
• 제24권2호
• /
• pp.155-160
• /
• 2013

미세조류의 지질축적을 증가시키는 것에 대한 돌연변이 생성 및 분리에 관한 연구는 바이오디젤 산업에 중요한 문제이다. 본 연구에서는, 광합성 미세조류인 Nannochloropsis oculata (N. oculata)를 이용하여 자외선(UV-B 타입)으로 돌연변이를 유도하였다. 그 결과 콜로니가 생성되었고, 그 이후에 f/2 액체배지와 고체배지에 배양하였다. 몇 주간 배양후, 변화된 세포성장률과 세포건조중량, 그리고 몇 가지 중요한 세포 구성 요소를 조사하였다. 수천 개의 변이주 중 두 개의 변이주가 야생균주에 비해서 증가된 세포성장과 높은 지질 축적을 보였다. 또한 증가된 세포성장률과 함께 단백질 과발현 현상이 관찰되었다. 그러나 돌연변이주의 클로로필 생합성의 감소를 확인 할 수 있었다.

• 한국에너지공학회:학술대회논문집
• /
• 한국에너지공학회 1995년도 춘계학술발표회 초록집
• /
• pp.82-87
• /
• 1995

Carbon dioxide fixation by microalgae has several advantages such as no requirements for the pretreatment over phycal and/or chemical $CO_2$ fixation processes. Chlorella sp. HA-1, fresh algae. and Chlorococcum littorale. marine algae, were used to do $CO_2$ fixation work. The experimental parameters were light intensity and $CO_2$ concentration. Chlorella sp. HA-1 has the maximum growth rate at 8 Klux and 10% $CO_2$ concentration. Chlorococcum littorale showed the maximum growth at similar conditions. The results indicated the feasibility of the Chlorella HA-1 and Chlorococcum littorale for mass cultivation using flue gas.

• 상하수도학회지
• /
• 제27권3호
• /
• pp.325-329
• /
• 2013

A result of estimating the effects of initial phosphorus concentration and nitrogen sources on removal of nitrogen and phosphorus in wastewater treatment using Scenedesmus dimorphus shows that there was no difference in microalgae growth amount and nitrogen removal amount by phosphorus concentration(5 ~ 60 mg/L). On the other hand, as initial phosphorus concentration increased, phosphorus removal amount was increased by luxury uptake of microalgae. Scenedesmus dimorphus preferred to ammonia when ammonia(40 mg/L) and nitrate(40 mg/L) are simultaneously presented and nitrogen removal rate of ammonia was high at approximately 20 mg/L/day.