• Fisheries and Aquatic Sciences
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• 제5권1호
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• pp.36-42
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• 2002

Microarrayer is used to make DNA chip and microarray that contain hundreds to thousands of immobilized DNA probes on surface of a microscope slide. This paper shows the develop-ment results for a printing type of microarrayer. It realizes a typical, low-cost and efficient microarrayer for generating low density micro array. The microarrayer is developed by using a prependicular type robot with three axes. It is composed of a computer-controlled three-axes robot and a pen tip assembly. The key component of the arrayer is the print-head containing the tips to immobilize cDNA, genomic DNA or similar biological material on glass surface. The robot is designed to automatically collect probes from two 96-well plates with up to 12 pens at the same time. To prove the performance of the developed microarrayer, we use the general water types of inks such as black, blue and red. The inks are distributed at proper positions of 96 well plates and the three color inks are immobilized on the slide glass under the operation procedure. As the result of the test, we can see that it has sufficient performance for the production of low integrated DNA chip consisted of 96 spots within $1cm^2$ area.

• 한국전자파학회논문지
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• 제13권9호
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• pp.964-970
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• 2002

본 논문에서는 도로지형에 따른 안테나 방사패턴 형성에 대하여 도심지 교차로형 모양의 방사패턴을 갖는 안테나의 설계제작에 그 목적이 있다. 본 논문에서 제안한 도로는 폭 60 m, 길이 200 m의 도심지 8차선 교차로이며 이에 적용할 수 있는 기지국용 안테나의 방사패턴으로 십자형 방사패턴을 제안하였다. 설계 방법으로는 임의의 방사패턴의 형성을 타원식의 합으로 구하였으며, 이에 Woodward-Lawson 방법을 이용한 표본화로 스트립 급전 6소자 모노폴 선형 어레이로 마이크로스트립 기판상에 구현하였다. 제특성 측정 결과, 설계 중심주파수 2 GHz에서 반사손실 -13.5 dB, -10 dB 대역폭 120 MHz(6 %)를 나타내었으며, 방사패턴은 1.95 GHz 대역에서는 $\pm$45$^{\circ}$에서의 도로의 길이 방향보다 -6 dB의 감소가 보여져 제안된 도심지 교차로형 방사패턴이 양호한 십자형 방사패턴이 유지됨을 확인하였다.

• 한국전산유체공학회지
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• 제18권2호
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• pp.85-92
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• 2013

In this study, we consider heat transfer enhancement in laminar channel flow by means of an infinite streamwise array of equispaced identical circular cylinders. This flow configuration can be regarded as a model representing a micro channel or an internal heat exchanger with cylindrical vortex generators. A numerical parametric study has been carried out by varying Reynolds number based on the bulk mean velocity and the cylinder diameter, and the gap between the cylinders and the channel wall. An immersed boundary method was employed to facilitate to implement the cylinders on a Cartesian grid system. No-slip condition is employed at all solid boundaries including the cylinders, and the flow is assumed to be periodic in the streamwise direction. Also, the Prandtl number is fixed as 0.7. For thermal boundary conditions on the solid surfaces, it is assumed that heat flux is constant on the channel walls, while the cylinder surfaces remain adiabatic. The presence of the circular cylinders arranged periodically in the streamwise direction causes a significant topological change of the flow, leading to heat transfer enhancement on the channel walls. The Nusselt number averaged on the channel wall is presented for the wide ranges of Reynolds number and the gap. A significant heat transfer enhancement is noticed when the gap is larger than 0.8, while the opposite is the case for smaller gaps. More quantitative results as well as qualitative physical explanations are presented to justify the effectiveness of varying the gap to enhance heat transfer from the channel walls.

• 천문학회보
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• 제35권1호
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• pp.39.2-39.2
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• 2010

In this paper, I present the domestic development of near infrared camera systems for the ground telescope and the space satellite. These systems are the first infrared instruments made for astronomical observation in Korea. KASINICS (KASI Near Infrared Camera System) was developed to be installed on the 1.8m telescope of the Bohyunsan Optical Astronomy Observatory (BOAO) in Korea. KASINICS is equipped with a $512{\times}512$ InSb array enable L band observations as well as J, H, and Ks bands. The field-of-view of the array is $3.3'{\times}3.3'$ with a resolution of 0.39"/pixel. It employs an Offner relay optical system providing a cold stop to eliminate thermal background emission from the telescope structures. From the test observation, limiting magnitudes are J=17.6, H=17.5, Ks=16.1 and L(narrow)=10.0 mag at a signal-to-noise ratio of 10 in an integration time of 100 s. MIRIS (Multi-purpose InfraRed Imaging System) is the main payload of the STSAT-3 in Korea. MIRIS Space Observation Camera (SOC) covers the observation wavelength from $0.9{\mu}m$ to $2.0{\mu}m$ with a wide field of view $3.67^{\circ}{\times}3.67^{\circ}$. The PICNIC HgCdTe detector in a cold box is cooled down below 100K by a micro Stirling cooler of which cooling capacity is 220mW at 77K. MIRIS SOC adopts passive cooling technique to chill the telescope below 200K by pointing to the deep space (3K). The cooling mechanism employs a radiator, a Winston cone baffle, a thermal shield, MLI of 30 layers, and GFRP pipe support in the system. Opto-mechanical analysis was made in order to estimate and compensate possible stresses from the thermal contraction of mounting parts at cryogenic temperatures. Finite Element Analysis (FEA) of mechanical structure was also conducted to ensure safety and stability in launching environments and in orbit. MIRIS SOC will mainly perform the Galactic plane survey with narrow band filters (Pa $\alpha$ and Pa $\alpha$ continuum) and CIB (Cosmic Infrared Background) observation with wide band filters (I and H) driven by a cryogenic stepping motor.

• 대한기계학회논문집B
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• 제38권12호
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• pp.999-1008
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• 2014

채널 내 회전하는 원형 실린더가 주기적으로 존재하는 경우 회전하는 실린더를 지나는 유동에 의한 채널 내 유동 특성 및 채널 벽에서의 열전달 효율증진을 파악하였다. 본 연구에서 사용된 유동 모델은 마이크로 채널, 열교환기 등에서 평판 사이의 열전달 효율을 높이기 위해 흔히 사용되는 와류 생성기의 가장 단순한 모델이다. 실린더와 채널 벽과의 간격 및 Re 수를 변화해가며 수치적 해석을 수행하였으며, 직교좌표계에서 채널 내 원형 실린더를 구현하기 위해 가상경계법이 적용 되었다. 채널 내 실린더가 회전하고 있는 경우, 실린더가 정지해 있는 경우에 비해 특히 실린더와 채널 벽과의 간격이 작아질수록 채널 벽에서의 열전달 효과는 더 높은 것으로 파악되었다.

• 한국화재소방학회논문지
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• 제13권1호
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• pp.37-47
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• 1999

부유중인 분진의 화재 및 용기 또는 파이프의 미세한 균열에서 비산되는 가연성 액체의 분무화재의 위험성은 착화후의 고속 확산과 높은 열방출율로 인하여 매우 높은 것으로 알려졌다. 이에 대한 연구는 주로 실험적으로나 또는 거시적인 관점의 해석으로 제한되어 왔다. 본 연구는 미시적인 관점의 해석으로서 분진 및 분무를 가연성 미세 액적으로 가정하여 그의 증발과 착화에 대하여 연구하였다. 첫 단계로서 일열의 액적 배열을 계산영역으로 하여, 비정상 이차원 보존방정식들을 적용하였다. 수치해석은 일반화된 비직교 좌표계를 사용하였고, 화학반응은 Arrhenius의 법칙에 의하여 반응속도가 제어되는 일단계 반응을 고려하였다. 계산결과는 액적 주위의 온도와 반응물질의 농도분포를 시간에 따라 보여준다. 주위의 산소가 증발하는 액적의 연료와 섞이기 시작하고 착화 조건에 다다르면, 급격한 발열반응이 예혼합된 가스로부터 일어나기 시작한다. 최대온도 영역은 점차적으로 액적 표면으로 이동하며 최대온도는 착화이후 급격히 상승한다. 연료와 산소의 농도는 최대온도 영역 근처에서 최소값을 보인다. 따라서 착화순간에는 예혼합연소의 양상을 띠는 것으로 나타났다. 이후에는 예혼합 가스의 소멸로 확산연소의 양상을 띠게 된다. 액적간의 거리는 중요한 요소로서 멀리 떨어져 있는 경우부터 액적간의 거리가 가까워지면 착화지연 시간이 줄여들어 착화가 빨리 일어나는 것으로 관찰되었다. 또한 착화 후에는 최대온도 영역이 일열의 중심선으로부터 멀어지는 것으로 나타났는데 이것은 중심부근의 산소가 먼저 소모되고 외부로부터의 산소공급도 화염에 의해 차단되어 나타나는 현상이다. 이번 연구로 미세적인 착화현상에 대한 이해를 높이게 되었고 추후 복잡한 배열에 대한 연구도 가능할 것이다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2012년도 제42회 동계 정기 학술대회 초록집
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• pp.251-251
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• 2012

A lot of studies are undergoing on the magneto-optical (MO) properties of patterned magnetic systems for the reason that they have potential application to information technology such as ultrahigh-speed computing. Moreover, they can be considered as the future candidates for high-density MO storage devices. Not only the technical aspects, but there have been also tremendous interests in studying their properties related to the fundamental physics. The MO Kerr-rotation effects (both in reflected and the diffracted modes) and the magnetic force microscopy (MFM) are very useful techniques to investigate the micromagnetic properties of such periodic structures. Hence, in this study, we report on the MO properties of bilayered Cobalt (Co)/ nickel (Ni) micro-patterned anti-dot arrays. Such a ferromagnetic structure was made by sequentially depositing co (40 nm)/Ni (5 nm) bilayer on a Si substrate. The anti-dot patterning with hole diameter of $1{\mu}m$ was done only on the upper Co layer using photolithography technique, while the Ni underlayer was kept uniform. The longitudinal Kerr rotation (LKR) of the zeroth- and the first-order diffracted beams were measured at an incidence of $30^{\circ}$ by using a photoelastic modulator method. The external magnetic field was applied perpendicularly to the reflected and the diffracted beams using an electromagnet capable of a maximum field of ${\pm}5$ kOe. Significantly, it was observed that the LKR of the first-order diffracted beam is nearly 4 times larger than that of the zeroth-order beam. The simulated results for the hysteresis loops matched qualitatively well with the experimentally obtained ones. In conjunction with the LKR, we also investigated the magnetic-domain structure by using a MFM system, which were analyzed to elucidate the origin of the enhanced MO rotation.

• Korean Chemical Engineering Research
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• 제46권6호
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• pp.1081-1086
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• 2008

양자 점을 이용한 QD-LED(Quantum Dot - Light Emitting Device)의 소자 제작을 하기 위해서는 양자 점의 균일한 배열이 중요하다. 핵-껍질(core-shell) 구조의 CdSe/ZnS 양자 점을 기판에 고 밀도, 고 균일도로 배열하기 위하여 두 종류의 분자 끈(molecular linker)을 사용하였고, 공정의 단순화와 비용 절감을 위하여 고분자 도장인 PDMS(polydimethylsiloxane)를 이용한 미세접촉인쇄방법으로 양자 점들을 배열하였다. $TiO_2/ITO$ 기판에 양자 점을 고정시켜주는 역할을 하는 분자 끈으로는 2-carboxyethylphosphonic acid(CAPO)를 사용하였고, 양자 점 사이의 인력을 향상시켜주는 분자 끈으로는 1,6-hexanedithiol(HDT)을 사용하였다. 양자 점들의 배열 특성을 주사전자현미경(SEM, scanning electron microscope)과 원자 힘 현미경(AFM, atomic force microscope)으로 분석하였고, 광 발광분광기(PL, photoluminescence spectroscope)로 발광특성을 측정하였다.

• 대한본초학회지
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• 제34권6호
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• pp.71-78
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• 2019

Objective : The purpose of the study was to identify expression profiling of miRNAs associated with cancers after treating allergen-removed Rhus Verniciflua Stokes and allergen-removed Rhus Verniciflua Stokes fumigaed Angelica gigas on leukemia cell lines. Methods : miRNA expression has been analyzed using miRNA array method through denaturation and hybridization after isolating the total RNA from leukemic cell line treated with 100 ㎍/㎖ of aRVS and aRVS-A each. Microarray expressions were interpreted as 'significant' on miRNAs when decreased less than 0.5 fold or increased more than 1.5 fold compared with the control group. Results : Among 158 miRNAs in total, 32 miRNAs were significantly presented in miRNAs expression. miRNA has been activated with a variety of genes for predicted targets, and the overexpressed miRNAs were categorized according to proliferation and metastasis of cancer in this study. The findings were reported that seven miRNAs (let-7b, miR-193a-5p, 296-3p, 26a, 22, 124a, 92b) showed significant expressions on proliferation and growth, seven miRNAs (miR-193a-5p, 26a, 200c, 183, 124a, 198, 210) presented meaningful expressions on invasion and metastasis, two miRNAs (let-7b, miR-210) were highly expressed on angiogenesis, five miRNAs (let-7b, miR-26a, 181d, 181c, 296-5p) related with apoptosis, and six miRNAs (let-7b, miR-200c, 183, 370, 124a, 191) were associated with prognosis of cancer and early diagnostic factors for cancer. Conclusion : The mechanism of miRNA takes a role in diagnosis, treatment, and prognotic factors for cancer as well. This study suggested that further detailed research on overexpression of specific miRNA should be carried out continuously in the future.

• Journal of Microbiology and Biotechnology
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• 제19권2호
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• pp.208-214
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• 2009

Steroid hormones have long been known to have a profound influence on the immune system. Although the functions of the nuclear receptors in the development of T cells are fairly well studied, the differential expression of these receptors in T helper cells is poorly understood. Here, we investigated the differential expression of nuclear receptors and coregulators in Th1 and Th2 cells by genome-wide micro array analysis. The result showed that several nuclear receptors and coregulators are differentially expressed in these cells. The result was confirmed by RT-PCR. The result showed that $RXR{\alpha}$ is highly expressed in Th2 cells. Overexpression of $RXR{\alpha}$ in a Jurkat human T cell line induced IL4 but not IFN-${\gamma}$ gene expression, suggesting that $RXR{\alpha}$ plays a selective role in Th1 and Th2 differentiation. In summary, these results suggest that Th1/Th2 differentiation is influenced by differential regulation of nuclear receptors and coregulators.