• Bioinformatics and Biosystems
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• v.1 no.1
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• pp.67-72
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• 2006

The aim of this paper is to discuss the effect of missing values in detecting differentially expressed genes in a cDNA microarray experiment in the context of a one sample problem. We conducted a cDNA micro array experiment to detect differentially expressed genes for the metastasis of colorectal cancer based on twenty patients who underwent liver resection due to liver metastasis from colorectal cancer. Total RNAs from metastatic liver tumor and adjacent normal liver tissue from a single patient were labeled with cy5 and cy3, respectively, and competitively hybridized to a cDNA microarray with 7775 human genes. We used $M=log_2(R/G)$ for the signal evaluation, where Rand G denoted the fluorescent intensities of Cy5 and Cy3 dyes, respectively. The statistical problem comprises a one sample test of testing E(M)=0 for each gene and involves multiple tests. The twenty cDNA microarray data would comprise a matrix of dimension 7775 by 20, if there were no missing values. However, missing values occur for various reasons. For each gene, the no missing proportion (NMP) was defined to be the proportion of non-missing values out of twenty. In detecting differentially expressed (DE) genes, we used the genes whose NMP is greater than or equal to 0.4 and then sequentially increased NMP by 0.1 for investigating its effect on the detection of DE genes. For each fixed NMP, we imputed the missing values with K-nearest neighbor method (K=10) and applied the nonparametric t-test of Dudoit et al. (2002), SAM by Tusher et al. (2001) and empirical Bayes procedure by $L\ddot{o}nnstedt$ and Speed (2002) to find out the effect of missing values in the final outcome. These three procedures yielded substantially agreeable result in detecting DE genes. Of these three procedures we used SAM for exploring the acceptable NMP level. The result showed that the optimum no missing proportion (NMP) found in this data set turned out to be 80%. It is more desirable to find the optimum level of NMP for each data set by applying the method described in this note, when the plot of (NMP, Number of overlapping genes) shows a turning point.

• Proceedings of the Korea Concrete Institute Conference
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• 2004.05a
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• pp.644-647
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• 2004

Modulus of rupture (MOR) and flexural toughness in hybrid fiber reinforced cement pastes mixed with micro-fiber (carbon fiber) and macro-fiber (steel fiber) and replaced with silica fume according to the fixed ratio were researched. Reinforcing efficiency in specimens were estimated by two factors, such as strengthening factor $(F_s)$ and toughening factor $(F_t)$, which were calculated from the analysis of variance (ANOVA) of the response values, such as MOR and absorbtion energy $(W_0)$. According to the experimental design by the fractional orthogonal array, nine hybrid fibrous reinforced paste series and one non-reinforced control paste were manufactured. Specimens of each series were tested by the INSTRON Inc. 8502(model) equipment in three-points bending and then measured the load-deflection response relationships. Considerable strengthening of cement pastes resulted in' the case of other factors without carbon fiber and toughening of cement pastes about all factors showed high. Based on the significance of factors related to response values from ANOVA, following assessments were available; $F_s$ or MOR: silica fume $\gg$ steel fiber $\gg$ carbon fiber; $F_t\;or\;W_0$: steel fiber > carbon fiber > silica fume. Optimized composition condition was estimated by steel fiber of $1.5\%$, carbon fiber of $0.5\%$ and silica fume $7.5\%$ in side of strengthening and steel fiber of $1.5\%$, carbon fiber of $0.75\%$ and silica fume $7.5\%$ in side of toughening.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2003.05a
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• pp.132-137
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• 2003

This performance is motivated to develop a tracking antenna system for receive the satellite broadcasting signal in the coast sailing ship. Therefore, this system is made to small size, light weight and simple operation which is must to low cost system for popularization of small size ship and adaptive possibilities with useful on a ship in the coast using 1 axis Az-mount. The antenna mount structure is a compact size and easy operation to the Az-axis type which is operated by step motor. The antenna unit is a domestic made plate type of patch array and ship's moving detection is using the gyro sensor for ship's moving control. We are designed to algorithm, which walking is abreast for step track and ship's moving compensation. Ship's moving compensation is adapted to the closed loop control method by detection from gyro sensor. This system is consisted of micro processor, ADC, comparative amplifier, step motor driver, mount mechanism and algorithm. We have analysised the tracking performance of prototype on sailing ship board.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.23 no.6
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• pp.481-486
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• 2010

ENIG (electroless Ni immersion gold) is one of surface finishing which has been most widely used in fine pitch SMT (surface mount technology) and BGA (ball grid array) packaging process. The reliability for package bondability is mainly affected by interfacial reaction between solder and surface finishing. Since the behavior of IMC (intermetallic compound), or the interfacial reaction between Ni and solder, affects to some product reliabilities such as solderability and bondability, understanding behavior of IMC should be important issue. Thus, we studied the properties of ENIG with P contents (9 wt% and 13 wt%), where the P contents is one of main factors in formation of IMC layer. The effect of P content was discussed using the results obtained from FE-SEM(field-emission scanning electron microscope), EPMA(electron probe micro analyzer), EDS(energy dispersive spectroscopy) and Dual-FIB(focused ion beam). Especially, we observed needle type irregular IMC layer with decreasing Ni contents under high P contents (13 wt%). Also, we found how IMC layer affects to bondability with forming continuous Kirkendall voids and thick P-rich layer.

• Tribology and Lubricants
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• v.30 no.3
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• pp.139-145
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• 2014

Nowadays, most micro-patterns are manufactured during flow line production. However, a conventional rotary chemical mechanical polishing (CMP) system has a limited throughput for the fabrication of large and flexible electronics. To overcome this problem, we propose a novel linear roll-CMP system for the planarization of large-area electronics. In this paper, we present a statistical analysis on the linear roll-CMP process of copper-clad laminate (CCL) to determine the impacts of process parameters on the material removal rate (MRR) and its non-uniformity (NU). In the linear roll-CMP process, process parameters such as the slurry flow rate, roll speed, table feed rate, and down force affect the MRR and NU. To determine the polishing characteristics of roll-CMP, we use Taguchi's orthogonal array L16 (44) for the experimental design and F-values obtained by the analysis of variance (ANOVA). We investigate the signal-to-noise (S/N) ratio to identify the prominent control parameters. The "higher is better" for the MRR and "lower is better" for the NU were selected for obtaining optimum CMP performance characteristics. The experimental and statistical results indicate that the down force and roll speed mainly affect the MRR and the down force and table feed rate determine the NU in the linear roll-CMP process. However, over 186.3 N of down force deteriorates the NU because of the bending of substrate. Roll speed has little relationship to the NU and the table feed rate does not impact on the MRR. This study provides information on the design parameter of roll-CMP machine and process optimization.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.22
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• pp.9927-9931
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• 2014

The objectives of this study were to examine serum periplakin expression in patients with urothelial carcinoma of the urinary bladder and in normal controls, and to examine relationships with clinicopathological findings. Detection of serum periplakin was performed in 50 patients and 30 normal controls with anti-periplakin antibodies using the automatic dot blot system, and a micro-dot blot array with a 256 solid-pin system. Levels in patients with urothelial carcinoma of the urinary bladder were significantly lower than those in normal controls (0.31 and 5.68, respectively; p<0.0001). The area under the receiver-operator curve level for urothelial carcinoma of the urinary bladder was 0.845. The sensitivity and specificity, using a cut-off point of 4.045, were 83.7% and 73.3%, respectively. In addition, serum periplakin levels were significantly higher in patients with muscle-invasive cancer than in those with nonmuscle-invasive cancer (P = 0.03). In multivariate Cox proportional hazards regression analysis, none of the clinicopathological factors was associated with an increased risk for progression and cancer-specific survival. Examination of the serum periplakin level may play a role as a non-invasive diagnostic modality to aid urine cytology and cystoscopy.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.12
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• pp.7501-7508
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• 2013

Background: Chronic myeloid leukemia (CML) is a myeloproliferative disorder of hematopoietic stem cell scarrying the Philadelphia (Ph) chromosome and an oncogenic BCR-ABL1 fusion gene. The tyrosine kinase inhibitor (TKI) of BCR-ABL1 kinase is a treatment of choice for control of CML. Objective: Recent studies have demonstrated that miRNAs within exosomes from cancer cells play crucial roles in initiation and progression. This study was performed to assess miRNAs within exosomes of K562 cells. Methods: miRNA microarray analysis of K562 cells and K562 cell-derived exosomes was conducted with the 6th generation miRCURYTM LNA Array (v.16.0). Gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were also carried out. GO terms and signaling pathways were categorized into 66 classes (including homophilic cell adhesion, negative regulation of apoptotic process, cell adhesion) and 26 signaling pathways (such as Wnt). Results: In exosomes, 49 miRNAs were up regulated as compared to K562 cells, and two of them were further confirmed by quantitative real-time PCR. There are differentially expressed miRNAs between K562 cell derived-exosomes and K562 cells. Conclusion: Selectively expressed miRNAs in exosomes may promote the development of CML via effects on interactions (e.g. adhesion) of CML cells with their microenvironment.

• Tunnel and Underground Space
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• v.28 no.4
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• pp.293-303
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• 2018

Monitoring is the act of collecting and analyzing accurate engineering information using various methods and instruments. The purposes of the monitoring are design verification, construction management, quality control, safety management, and diagnose of structure etc.. The diagnose evaluation of the geotechnical structures corresponds to the confirmation of the structural performance. It is aimed to judge the soundness of geotechnical structures considering the degree of damage due to the environmental change and elapsed time. Recently, microseismicity, which is widely known in Korea, can be used for safety management and diagnoses of structure as it detects the micro-damage without disturbance of the structure. This report provides guideline on the procedure for assessing an underground oil storage cavern using microseismic monitoring techniques. Guidelines cover the selection of monitoring systems, sensor array, sensor installation and operation of systems, and interpretation.

• The Journal of the Korea institute of electronic communication sciences
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• v.6 no.6
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• pp.965-971
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• 2011

As the liver cancer in our country cancerous occurrence frequency to be the gastric cancer in the common cancer, If the case which will be discovered in early rising the treatment record was considered seriously about under the early detection. The system which it sees with the system for the early detection of the liver cancer reacts the blood of the control group other than the patient who is confirmed as the liver cancer and the liver cancer to the biochip and aptamer protein biochip profiles mechanical studying leads and it is a system which it classifies. 1149 each other it reacted blood samples of the control group other than the liver cancer patient who is composed of the total 85 samples and the liver cancer which is composed of 310 samples to the biochip which is composed with different oligo from the present paper and it was a data which it makes acquire worker the neural network it led and it analyzes the classification efficiency of the result 95.38 ~ 97.95% which it was visible.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.7 no.6
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• pp.1130-1137
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• 2003

This performance is motivated to develop a tracking antenna system for receive the satellite broadcasting signal in the coast sailing ship. Therefore, this system is made to small size, light weight and simple operation which is must to low cost system for popularization of small size ship and adaptive possibilities with useful on a ship in the coast using 1 axis Az-mount. The antenna mount structure is a compact size and easy operation to the Az-axis type which is operated by step motor. The antenna unit is a domestic made plate type of patch array and ship's moving detection is using the gyro sensor for ship's moving control. We are designed to algorithm, which walking is abreast for step track and ship's moving compensation. Ship's moving compensation is adapted to the closed loop control method by detection from gyro sensor. This system is consisted of micro processor, ADC, comparative amplifier, step motor driver, mount mechanism and algorithm. We have analysised the tracking performance of prototype on sailing ship board.