• KIEE International Transactions on Electrophysics and Applications
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• v.4C no.4
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• pp.176-180
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• 2004

This paper presents the fabrication and test of a micro cell exciter actuated by an electromagnetic force for the study on the chondrogenic differentiation of rabbit mesenchymal stem cells (MSCs). The micro cell exciter is designed to apply compressive loading to the alginate gel mixed with the MSCs. The magnetic cell exciter consists of an actuator component and a cartridge-type chamber component. An actuator is composed of a permanent magnet, a core and a coil. The chamber has seven PMMA wells and a cell culture Petri dish. Two types of alginate gels were stimulated by the cell exciters for 10 minutes every 12 hours for 7 days. In order to determine the expression of these matrix components during differentiation, RT-PCR analysis was performed. Collagen type II was expressed in the MSCs subjected to the compressive stimulation.

• Journal of Sensor Science and Technology
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• v.10 no.4
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• pp.232-238
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• 2001

We fabricated micro-pump using stereolithography technology. The pump was fabricated using PZT for its drive and used non-movable valve of diffuser/nozzle concept. Size of the pump chamber is, $14mm{\psi}$, narrow hole of diffuser/nozzle is $0.5mm{\psi}$ and wide hole $1mm{\psi}$ and chamber depth is 1.2mm, respectively. We confirmed its operation at frequency 100Hz, supply voltage $90{\sim}250Vp-p$. As the result of the pump working measurement, when ethanol is used, the pressure head difference between inlet and outlet of the pump is about 53mm in the frequency of 100Hz, 200V of Vp-p.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2009.06a
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• pp.185-185
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• 2009

Micropump is very useful component in micro/nano fluidics and bioMEMS applications. Using the flexural vibration mode of PZT bar, a piezopump is successfully made. The PZT bar is polarized with thickness direction. The proposed structure for the piezo-pump consists of an input and an output port, piezoelectric ceramic actuator, actuator support, diaphragm. The traveling flexural wave along the bar is obtained by dividing two standing waves which are temporally and spatially phase shifted by 90 degrees from each other. Fluid is drawn into a forming chamber, eventually the forming chamber closes trapping the fluid therein. The finite elements analysis on the proposed pump model is carried out to verify its operation principle and design by the commercial FEM software. Components of piezopump were made, assembled, and tested to validate the concepts of the proposed pump and confirm the simulation results. The performance of the proposed piezopump the highest pressure level of 83.4kHz.

• Proceedings of the KIEE Conference
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• 2003.10a
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• pp.45-48
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• 2003

In the research of proteomics, mass spectrometry analysis is the essential method for identification of the unknown proteins. Trypsin treatment for the sample preparation of mass spectrometry is the inevitable procedure[1]. However, sample preparation procedure is cumbersome and time consuming. To resolve these problems, Temperature controllable microreactor was designed and fabricated. It consists of metering chamber, micro channel, reaction chamber, platinum (Pt) thin film heater and a temperature sensor so that micro metering and mixture of reagent with temperature control can be done on the same chip. The total size of the fabricated microreactor was $37{\times}30{\times}1\;mm^3$ and the size of channel cross section was $200{\times}100{\mu}m^2$. PID temperature controller was realized using NI DAQ, PCI-MIO-l6E-1 board and LabVIEW program.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.32 no.9
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• pp.720-727
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• 2008

Microfluidic single chip integrating thermopneumatic micropump and micro check valve are developed. The micropump and micorvalve are made of biocompatible materials, glass and PDMS, so as to be applicable to the biochip. By using the passive-type check valve, backward flow and fluid leakage are blocked and flow control is stable and precise. The chip is composed of three PDMS layers and a glass substrate. In the chip, flow channel and pump chamber were made on the PDMS layers by the replica molding technique and pump heater was made on the glass substrate by Cr/Au deposition. Diameter of the pump chamber is 7 mm and the width and depth of the channel are 200 and $180{\mu}m$, respectively. The PDMS layers chip and the heater deposited glass chip are combined by a jig and a clamp for pumping operation, and they are separable so that PDMS chip is used as a disposable but the heater chip is able to be used repeatedly. Pumping performance was simulated by CFD software and investigated experimentally. The performance was the best when the duty ratio of the applied voltage to the heater was 33%.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.30 no.8 s.251
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• pp.722-730
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• 2006

In this study, the flow characteristics of a FPN (Fountain Pen Nano-Lithography) using active membrane pumping are investigated. The FPN has integrated chamber, micro channel, and high capacity reservoir for continuous ink feed. The most important aspect in this probe provided control of fluid injection using active membrane pumping in chamber. The flow rates in channel by capillary force are theoretically analyzed, including the control of the mass flow rates by the deflection of the membrane. The above results are compared with the numerical simulations that calculated by commercial code, FLUENT. The velocity of the fluid in micro channel shows linear behaviors. And the mass flows are proportional to the second order function of the pumping pressure that is imposed to the membrane.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2002.05a
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• pp.932-937
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• 2002

The polyimide nozzle and silicon restrictor inside a thermal micro actuator have been fabricated using state of the art laser micromachining methods. Numerical models of fluid dynamics inside the actuator chamber and nozzle are presented. The models include fluid flow from reservoir, bubble formation and growth, ejection through the nozzle, and dynamics of refill through restrictor. Since high tapered nozzle and restrictor are very important parameters for overall actuator performance design, a special setup for the beam delivery system has been developed. The effects of variations of nozzle thickness, diameter, taper angles, and restrictor shapes are simulated and some results are compared with the experimental results. It is fecund that the fluid ejection through the thinner and high tapered nozzle is more steady, fast, and robust and the tapered restrictor shows more satisfying refill than the zero taper one.

• Journal of Advanced Marine Engineering and Technology
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• v.38 no.10
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• pp.1232-1236
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• 2014

Numerical models of fluid dynamics inside the micro actuator chamber and nozzle are presented. The models include ink flow from reservoir, bubble formation and growth, ejection through the nozzle, and dynamics of refill process. Since high tapered nozzle is one of the very important parameters for overall actuator performance design. The effects of variations of nozzle thickness, diameter, and taper angles are simulated and some results are compared with the experimental results. It is found that the ink droplet ejection through the thinner and high tapered nozzle is more steady, fast, and robust.

• Bulletin of the Korean Chemical Society
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• v.34 no.1
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• pp.139-142
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• 2013

A study was carried out to evaluate the solid phase micro-extraction (SPME) for formaldehyde emission analysis of uncoated plywood. In SPME, formaldehyde was on-fiber derivatized through headspace extraction and analyzed by gas chromatography coupled with mass spectrometry (GC/MS). The SPME was compared with desiccators (DC-JAS 233), small-scale chamber (SSC-ASTM D6007) and liquid-liquid extraction (LLE-EPA 556) methods which were performed in accordance with their respective standards. Compared to SSC (RSD 4.3%) and LLE (RSD 5.0%), the SPME method showed better repeatability (RSD 1.8%) and not much difference from DC (RSD 1.4%). The SPME has proven to be highly precise (at 95% confidence level) with better recovery (REC 102%). Validation of the SPME method for formaldehyde quantitative analysis was evidenced. In addition, the SPME by air sampling directly from plywood specimens (SPME-W) correlated best with DC ($r^2$ = 0.983), followed by LLE ($r^2$ = 0.950) and SSC ($r^2$ = 0.935).

• Current Optics and Photonics
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• v.8 no.2
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• pp.170-182
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• 2024

The dimensional accuracy and consistency of a dual oil circuit centrifugal fuel nozzle are important for fuel distribution and combustion efficiency in an engine combustion chamber. A point cloud measurement method was proposed to solve the geometric accuracy detection problem for the fuel nozzle. An improved variance focus measure operator was used to extract the depth point cloud. Compared with other traditional sharpness evaluation functions, the improved operator can generate the best evaluation curve, and has the least noise and the shortest calculation time. The experimental results of point cloud slicing measurement show that the best window size is 24 × 24 pixels. In the height measurement experiment of the standard sample block, the relative error is 2.32%, and in the fuel nozzle cone angle measurement experiment, the relative error is 2.46%, which can meet the high precision requirements of a dual oil circuit centrifugal fuel nozzle.