• Journal of Genetic Medicine
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• v.8 no.2
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• pp.119-124
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• 2011

Purpose: Supernumerary marker chromosome (SMC) could be associated with various phenotypic abnormalities based on the chromosomal origin of SMCs. The present study aimed to determine the genomic contents of SMCs using chromosomal microarray and to analyze molecular cytogenetic characterizations and clinical phenotypes in patients with SMCs. Materials and Methods: Among patients with SMCs detected in routine chromosomal analysis, SMCs originating from chromosome 15 were excluded from the present study. CGH-based oligonucleotide chromosomal microarray was performed in 4 patients. Results: The chromosomal origins of SMCs were identified in 3 patients. Case 1 had a SMC of 16.1 Mb in 1q21.1-q23.3. Case 2 showed 21 Mb gain in 19p13.11-q13.12. Case 3 had a 4.5 Mb-sized SMC rearranged from 2 regions of 2.5 Mb in 22q11.1-q11.21 and 2.0 Mb in 22q11.22-q11.23. Conclusion: Case 1 presented a wide range of phenotypic abnormalities including the phenotype of 1q21.1 duplication syndrome. In case 2, Asperger-like symptoms are apparently related to 19p12-q13.11, hearing problems and strabismus to 19p13.11 and other features to 19q13.12. Compared with cat-eye syndrome type I and 22q11.2 microduplication syndrome, anal atresia in case 3 is likely related to 22q11.1-q11.21 while other features are related to 22q11.22-q11.23. Analyzing SMCs using high-resolution chromosomal microarray can help identify specific gene contents and to offer proper genetic counseling by determining genotype-phenotype correlations.

• Journal of Korean Society of Archives and Records Management
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• v.1 no.1
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• pp.231-243
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• 2001

The sterilization method of records by fumigation using mixed gas of methyl bromide(MB) and ethylene oxide(ETO) has been generally used as a way to protect biological deterioration by microorganisms and insects. In this study, we confirmed sterilization effect of MB and ETO [86 : 14(Vol.%)] on and analyzed toxicity on microorganisms isolated from records. To analyze sterilization effect of fumigation on microorganisms and insect, we have fumigated microorganisms and insect with a various amounts mixed gas of MB and ETO, and various exposure time. Insect was sterilized at all experimental conditions. In microorganisms, sterilization effect was detected only when the mixed gas was treated at $120g/m^3$ concentration for at least 24 hrs. To test the possibility of isolated microorganisms as a threat to human health, it was investigated that toxicity test using yeast, radish and cancer cells on microorganisms. Only Aspergillus oryzae had an inhibition effect on growth of yeast. radish and cancer cells, respectively. These results demonstrate that sterilization effect can occur at low concentrations of the mixed gas on insect but requires higher concentrations of the mixed gas on microorganisms. In addition to, it is suspected that the possibility of the microorganism as a threat to human health is little.

• Journal of Biomedical Engineering Research
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• v.40 no.2
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• pp.39-47
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• 2019

Ultrasound sonication along with microbubble (MB) could enhance drug delivery to promote the absorption of anticancer drugs into cancers in a noninvasive and targeted manners. In this study, we verify the acute drug delivery enhancement (within an hour) of two representative focused ultrasound driven drug delivery enhancement methods (MB and Doxorubicin-coated Nanoparticle complex (MB-NP) based). Experiments were conducted using in vivo mouse model with MDA-MB-231 breast cancer cell line. Ultrasound generated by single-element 1 MHz focused ultrasound transducer was delivered in pulsed sonication consisted of 0.125 msec bursts at a pulse repetition frequency of 2 Hz for 20 seconds without a significant increase in local temperature (less than $0.1^{\circ}C$) or hemorrhage. Doxorubicin concentrations in tumors were improved by 1.97 times in the case of MB-NP, and 1.98 times by using Doxorubicin and MB separately. These results indicate anticancer drug delivery based on MB and MB-NP can significantly improve the effect of anticancer drugs delivered to tumors in a short time period by using low-intensity focused ultrasound.

• Korean Journal of Optics and Photonics
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• v.7 no.1
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• pp.72-76
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• 1996

The magnetostrictive characteristics of three different forms of magnetostrictive transducers made of 2605SC and 2826MB metallic glasses were measured. In both cases, the two-end-point bonding configuration exhibited the best characteristics. With the two-end-point bonding we measured the effective magnetostrictive coefficient $C_{eff}$ of $1.2{\times}^{-5}Oe^{-2}$, the saturation magnetostriction ${\lambda}_s$ of $7.4{\times}10^{-4}$ and the minimum detectable magnetic field $H_{min}$ of $1.6{\times}10^{-7}Oe$/ √Hz for the 2605SC metallic glass ribbon. Also for the 2826MB metallic glass ribbon, Ceff and ${\lambda}_s$ were $7.6{\times}10^{-6}Oe^{-2}$ and $3.4{\times}10^{-4}$ respectively.

• Natural Product Sciences
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• v.28 no.3
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• pp.115-120
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• 2022

Ten compounds, consisting of neoflavonoids (1-5), isoflavonoids (6 and 7), flavanone (8), and chalcones (9 and 10) were isolated from the ethyl acetate and n-butanol-soluble fractions of the heartwood of Dalbergia melanoxylon. The chemical structures were identified on the basis of spectroscopic evidence and compared to previously reported spectra. Compounds 1-10 were evaluated for cytotoxicity against HCT116 human colorectal cancer, MDA-MB-231 human metastatic breast cancer, and A2058 human melanoma cell lines. Among them, compounds 3 and 10 showed the strongest cytotoxic activity with IC₅₀ values of 11.92±1.07 μM, 10.83±1.02 μM, and 14.37±1.02 μM, 13.62±1.09 μM against HCT116 and MDA-MB-231 cell lines, respectively. Compounds 9 and 10 also had cytotoxic activity with IC₅₀ values of 13.49±1.18 μM and 9.82±0.91 μM against A2058 cell lines, respectively. To the best our knowledge, compounds 2 and 5-10 were isolated from this source for the first time.

• Journal of the Optical Society of Korea
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• v.16 no.1
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• pp.1-5
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• 2012

This paper describes a 150-Mb/s monolithic optical receiver for plastic optical fiber link using a standard CMOS technology. The receiver integrates a photodiode using an N-well/P-substrate junction, a pre amplifier, a post amplifier, and an output driver. The size, PN-junction type, and the number of metal fingers of the photodiode are optimized to meet the link requirements. The N-well/P-substrate photodiode has a 200-${\mu}m$ by 200-${\mu}m$ optical window, 0.1-A/W responsivity, 7.6-pF junction capacitance and 113-MHz bandwidth. The monolithic receiver can successfully convert 150-Mb/s optical signal into digital data through up to 30-m plastic optical fiber link with -10.4 dBm of optical sensitivity. The receiver occupies 0.56-$mm^2$ area including electrostatic discharge protection diodes and bonding pads. To reduce unnecessary power consumption when the light is not over threshold or not modulating, a simple light detector and a signal detector are introduced. In active mode, the receiver core consumes 5.8-mA DC currents at 150-Mb/s data rate from a single 3.3 V supply, while consumes only $120{\mu}W$ in the sleep mode.

• ETRI Journal
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• v.9 no.2
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• pp.96-105
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• 1987

The purpose of this paper is to report the implementation of new frame synchroniazation circuit for D3/PCM.When the D3/PCM signal(1.544Mb/s) is received, the performance of the new frame synchronizer is evaluated by the statistical method according to the parameters BER,es, em. For BER=$10^-2$, es=0 and em=2, the implemented hardware will render the initial search time, the maintenance time and there frame time equals to 2.87ms, 9.9hrs and2.69ms, respectively. Also the proposed frame synchronizer can be easily modified to the practical transmission channels environment and the new 1.544Mb/s signal format.

• YAKHAK HOEJI
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• v.35 no.1
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• pp.22-29
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• 1991

pMB4 is a 2.4-kilobase plasmid of Staphylococcus aureus TR-1 that confers inducible resistance to the macrolide-lincosamide-streptogramin B(MLS) antibiotics. By subcloning studies, it was found that the MLS resistance determinant was located at 1.0Kb fragment between Sau3AI and TaqI sites. DNA sequence of the MLS resistant determinant, named ermC-4 was determined, and found to be highly homologous with that of ermC. Because the leader peptide sequence of ermC-4 was identical with that of ermC, the expression of the resistance gene is thought to be controlled by posttranscriptional attenuation in S. aureus TR-1.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.20 no.12
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• pp.1297-1307
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• 2009

The purpose of this paper is to propose a detection algorithm and a tracking algorithm based on silent time using MB-OFDM UWB(Multi-Band Orthogonal Frequency Division Multiplexing Ultra Wide Band) receiver in order to satisfy DAA(Detect And Avoid) regulation of Korea to permit UWB in 3.1~4.8 GHz. In DAA regulation of Korea, if UWB device receives a signal more than -80 dBm/MHz from the victim system during UWB operation, the UWB system should avoid the collision within 2 sec. In this paper, we proposed the detection algorithm to detect the victim signal received by －80 dBm/MHz for the avoidance process that changes the operating UWB frequency to other UWB frequency and the subcarrier tracking algorithm to follow up the subcarrier positions of the victim signal for the tonenulling avoidance process that decreases the TX power of subcarriers occupied by the victim signal by -70 dBm/MHz. The performance of the detection algorithm and the tracking algorithm suggested in this paper is verified in simulation results considering various conditions.

• The Journal of Korean Obstetrics and Gynecology
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• v.28 no.2
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• pp.26-39
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• 2015

Objectives : Dendropanax morbifera is known as a tree that has been used in traditional medicine for various diseases. However, its biological activities in cancer have not yet been clearly elucidated. In this study, we investigated the anti-cancer effects of water extract of Dendropanax morbifera (DP) on 2 human breast cancer cell lines (estrogen dependent MCF-7 and estrogen independent MDA-MB-231). Methods : The MTT assay and flow cytometry were used to assess cell proliferation, along with cell cycle analysis. Nitric oxide production was detected by Griess assay. The expression of apoptosis related gene was assessed by quantitative real-time PCR. Results : Our data revealed that DP inhibits the cell growth in a dose dependent manner (0, 50, 100, 250, and 500 μg/ml) of both estrogen independent MDA-MB-231 and estrogen dependent MCF-7 breast cancer cells. Also, LPS induced nitric oxide production was significantly reduced by DP. Cell cycle analysis showed an increased G1 phase in the MCF-7 cell and G2/M phase in the MDA-MB-231 cell. DP decreased mRNA expression of apoptotic suppressor gene Bcl-xL, and increased mRNA expression of pro-apoptotic genes. DP increased mRNA expression of p21, and Rip1 in both cell. And DP decreased mRNA expression of survivin in the MCF-7 cell. Conclusions : Taken together, these results indicate that DP extract are source of anti-cancer potential and could be developed botanical drug.