• BMB Reports
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• 제51권9호
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• pp.456-461
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• 2018

Chemoresistance is a major barrier to successful cisplatin-based chemotherapy for epithelial ovarian cancer (EOC), and emerging evidences suggest that microRNAs (miRNAs) are involved in the resistance. In this study, it was indicated that miR-363 downregulation was significantly correlated with EOC carcinogenesis and cisplatin resistance. Moreover, miR-363 overexpression could resensitise cisplatin-resistant EOC cells to cisplatin treatment both in vitro and in vivo. In addition, data revealed that EMT inducer Snail was significantly upregulated in cisplatin-resistant EOC cell lines and EOC patients and was a functional target of miR-363 in EOC cells. Furthermore, snail overexpression could significantly attenuate miR-363-suppressed cisplatin resistance of EOC cells, suggesting that miR-363-regulated cisplatin resistance is mediated by snail-induced EMT in EOC cells. Taken together, findings suggest that miR-363 may be a biomarker for predicting responsiveness to cisplatin-based chemotherapy and a potential therapeutic target in EOC.

• The Korean Journal of Physiology and Pharmacology
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• 제18권5호
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• pp.359-363
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• 2014

MicroRNAs (miRs) are endogenous ${\approx}22$-nt non-coding RNAs that participate in the regulation of gene expression at post-transcriptional level. MiR-1 is one of the muscle-specific miRs, aberrant expression of miR-1 plays important roles in many physiological and pathological processes. In this review, we focus on the recent studies about miR-1 in cardiac diseases and cancers. The findings indicate that miR-1 may be a novel, important biomarker, and a potential therapeutic target in cardiac diseases and cancers.

• BMB Reports
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• 제52권3호
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• pp.226-226
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• 2019

• 디지털융복합연구
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• 제16권3호
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• pp.363-373
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• 2018

본 연구는 조혈모세포이식병동 간호사의 자율성과 직무만족도를 파악하고, 자율성과 직무만족도와의 상관관계를 규명하기 위한 조사연구이다. 국내 7개의 대학병원에 근무하는 간호사 79명을 대상으로 2013년 5월 20일부터 6월 4일까지 설문조사를 실시하였다. 분석방법은 SPSS 14.0을 이용하여 t-test, ANOVA, Pearson correlation coefficient를 실시하였다. 자율성 평균은 $165.47{\pm}19.56$점, 직무만족도 평균은 $3.15{\pm}0.35$점으로 나타났다. 자율성과 직무만족도는 상관관계를 보이지 않았으나, 직무만족도의 하부영역 중 자율성영역(r=.273)과 상호작용영역(r=.257)은 자율성과 유의한 정적 상관관계를 보였다. 이에 조혈모세포이식병동 간호사의 직무만족도 향상을 위해서 직무만족도의 하부영역인 자율성을 높일 필요가 있을 것이다. 따라서 본 연구결과는 조혈모세포이식병동 간호사들의 자율성과 직무만족도를 증가시킬 수 있는 교육과 정책프로그램을 개발에 유용한 기초자료로 이용될 수 있다.

• Journal of Dental Anesthesia and Pain Medicine
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• 제17권4호
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• pp.307-312
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• 2017

Background: The aim of this study was to estimate the optimal depth of nasotracheal tube placement. Methods: We enrolled 110 patients scheduled to undergo oral and maxillofacial surgery, requiring nasotracheal intubation. After intubation, the depth of tube insertion was measured. The neck circumference and distances from nares to tragus, tragus to angle of the mandible, and angle of the mandible to sternal notch were measured. To estimate optimal tube depth, correlation and regression analyses were performed using clinical and anthropometric parameters. Results: The mean tube depth was $28.9{\pm}1.3cm$ in men (n = 62), and $26.6{\pm}1.5cm$ in women (n = 48). Tube depth significantly correlated with height (r = 0.735, P < 0.001). Distances from nares to tragus, tragus to angle of the mandible, and angle of the mandible to sternal notch correlated with depth of the endotracheal tube (r = 0.363, r = 0.362, and r = 0.546, P < 0.05). The tube depth also correlated with the sum of these distances (r = 0.646, P < 0.001). We devised the following formula for estimating tube depth: $19.856+0.267{\times}sum$ of the three distances ($R^2=0.432$, P < 0.001). Conclusions: The optimal tube depth for nasotracheally intubated adult patients correlated with height and sum of the distances from nares to tragus, tragus to angle of the mandible, and angle of the mandible to sternal notch. The proposed equation would be a useful guide to determine optimal nasotracheal tube placement.

• 분석과학
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• 제26권6호
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• pp.357-363
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• 2013

Selenium-containing proteins were separated from selenium-enriched yeast (SEY) using Trizol$^{(R)}$ reagent followed by anion exchange (AE) chromatography. This method is simpler and less time consuming than electrophoresis. Five selenium containing proteins were identified by on-line AE HPLC-ICP/MS (high performance liquid chromatography-inductively coupled plasma/mass spectrometry). Each protein was enzymatically hydrolyzed to seleno-amino acids and separated with RP (reverse phase) HPLC for the identification of selenoproteins.

• Biomolecules & Therapeutics
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• 제21권5호
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• pp.358-363
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• 2013

In the present study, we investigated the effect of intracellular glutathione (GSH) depletion in heart-derived H9c2 cells and its mechanism. L-buthionine-S,R-sulfoximine (BSO) induced the depletion of cellular GSH, and BSO-induced reactive oxygen species (ROS) production was inhibited by glutathione monoethyl ester (GME). Additionally, GME inhibited BSO-induced caspase-3 activation, annexin V-positive cells, and annexin V-negative/propidium iodide (PI)-positive cells. Treatment with rottlerin completely blocked BSO-induced cell death and ROS generation. BSO-induced GSH depletion caused a translocation of PKC-${\delta}$ from the cytosol to the membrane fraction, which was inhibited by treatment with GME. From these results, it is suggested that BSO-induced depletion of cellular GSH causes an activation of PKC-${\delta}$ and, subsequently, generation of ROS, thereby inducing H9c2 cell death.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제33권4호
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• pp.363-367
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• 2011

Genioplasy with osteotomy is a flexible and useful procedure. However, osteotomy can cause different types of morbidity. Chin augmentation with porous polyethylene ($Medpor^{(R)}$) has become popular in the recent years. Porous polyethylene ($Medpor^{(R)}$) is an excellent biomaterial for reconstructing facial deformities. $Medpor^{(R)}$ has a porous architecture, which prevents capsule formation and decreases the degree of foreign-body reaction. In addition, it can be easily cut with scissors and molded, and it also maintains its shape. We report here on a satisfactory case of chin augmentation with using porous polyethylene ($Medpor^{(R)}$).

• Journal of Breast Cancer
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• 제21권4호
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• pp.363-370
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• 2018

Purpose: Breast cancer is the most commonly occurring cancer among women worldwide, and therefore, improved approaches for its early detection are urgently needed. As microRNAs (miRNAs) are increasingly recognized as critical regulators in tumorigenesis and possess excellent stability in plasma, this study focused on using miRNAs to develop a method for identifying noninvasive biomarkers. Methods: To discover critical candidates, differential expression analysis was performed on tissue-originated miRNA profiles of 409 early breast cancer patients and 87 healthy controls from The Cancer Genome Atlas database. We selected candidates from the differentially expressed miRNAs and then evaluated every possible molecular signature formed by the candidates. The best signature was validated in independent serum samples from 113 early breast cancer patients and 47 healthy controls using reverse transcription quantitative real-time polymerase chain reaction. Results: The miRNA candidates in our method were revealed to be associated with breast cancer according to previous studies and showed potential as useful biomarkers. When validated in independent serum samples, the area under curve of the final miRNA signature (miR-21-3p, miR-21-5p, and miR-99a-5p) was 0.895. Diagnostic sensitivity and specificity were 97.9% and 73.5%, respectively. Conclusion: The present study established a novel and effective method to identify biomarkers for early breast cancer. And the method, is also suitable for other cancer types. Furthermore, a combination of three miRNAs was identified as a prospective biomarker for breast cancer early detection.

• 디지털융복합연구
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• 제18권6호
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• pp.363-371
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• 2020

본 연구는 간호대학생의 그릿과 회복탄력성이 임상실습 적응에 미치는 효과를 보기 위한 조사연구이다. 일개 대학 간호학과 학생 131명을 대상으로 하였고, 자료수집은 2019년 8월 19일부터 9월 30일까지 수행하였다. 수집된 자료의 분석은 SPSS 22.0 프로그램을 사용하여 기술통계, t-test, 상관관계 및 다중회귀분석으로 분석하였다. 그 결과 그릿(r=.22, p=.012)과 회복탄력성(r=.56, p=<.001)은 임상실습 적응과 유의한 양의 상관관계를 나타내었다. 그릿과 회복탄력성은 간호대학생의 임상실습 적응에 영향을 미치는 요인으로 나타났으며, 그릿과 회복탄력성은 임상실습 적응을 32.0%(F=31.567, p=<.001) 설명하였다. 그러므로 간호대학생의 임상실습 적응을 높이기 위해서는 그릿과 회복탄력성을 향상시키기 위한 노력과 이를 적용한 임상실습 교육과정 개발이 필요하다.