• Title/Summary/Keyword: Mg utilization

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Pig Skin Gelatin Hydrolysates Attenuate Acetylcholine Esterase Activity and Scopolamine-induced Impairment of Memory and Learning Ability of Mice

  • Kim, Dongwook;Kim, Yuan H. Brad;Ham, Jun-Sang;Lee, Sung Ki;Jang, Aera
    • Food Science of Animal Resources
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    • v.40 no.2
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    • pp.183-196
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    • 2020
  • The protective effect of pig skin gelatin water extracts (PSW) and the low molecular weight hydrolysates of PSW generated via enzymatic hydrolysis with Flavourzyme® 1000L (LPSW) against scopolamine-induced impairment of cognitive function in mice was determined. Seventy male ICR mice weighing 20-25 g were randomly assigned to seven groups: Control (CON); scopolamine (SCO, 1 mg/kg B.W., intraperitoneally (i.p.); tetrahydroaminoacridine 10 [THA 10, tacrine; 10 mg/kg B.W. per oral (p.o.) with SCO (i.p.)]; PSW 10 (10 mg/kg B.W. (p.o.) with SCO (i.p.); PSW 40 (40 mg/kg B.W. (p.o.) with SCO (i.p.); LPSW 100 (100 mg/kg B.W. (p.o.) with SCO (i.p.); LPSW 400 (400 mg/kg B.W. (p.o.) with SCO (i.p.). All treatment groups, except CON, received scopolamine on the day of the experiment. The oxygen radical absorbance capacity of LPSW 400 at 1 mg/mL was 154.14 μM Trolox equivalent. Administration of PSW and LPSW for 15 weeks did not significantly affect on physical performance of mice. LPSW 400 significantly increased spontaneous alternation, reaching the level observed for THA and CON. The latency time of animals receiving LPSW 400 was higher than that of mice treated with SCO alone in the passive avoidance test, whereas it was shorter in the water maze test. LPSW 400 increased acetylcholine (ACh) content and decreased ACh esterase activity (p<0.05). LPSW 100 and LPSW 400 reduced monoamine oxidase-B activity. These results indicated that LPSW at 400 mg/kg B.W. is a potentially strong antioxidant and contains novel components for the functional food industry.

Analysis of Chemical Components of Xylem Sap from 'Hayward' Kiwifruit Canes and Processing of Drink Using the Xylem Sap (참다래 'Hayward' 수액의 화학성분 분석 및 수액을 이용한 음료 제조)

  • Park, Yong Seo;Lim, Keun Cheol;Lee, Ji Heon
    • Horticultural Science & Technology
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    • v.18 no.6
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    • pp.808-810
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    • 2000
  • The chemical components of xylem sap collected from kiwifruit (Actinidia chinensis Planch cv. Hayward) trees were analyzed and processing of xylem sap drink were accomplished to increase utilization of xylem sap as a drink. Water content, soluble solids, viscosity, and pH of the xylem sap were 99.60%, 0.90%, 1.01, and 6.50, respectively. In the xylem sap, fructose was the highest among free sugars followed by glucose, sucrose, galactose, and mannitol. The major inorganic components in the sap were calcium, potassium, and magnesium, and calcium was the dominant among them. Ten kinds of amino acids in the xylem sap were detected by amino acid analyzer, and the major amino acids were glutamic acid, lysine, and isoleucine. Glutamic acid was the most dominant amino acid in the xylem sap. Major compositions of xylem sap drink were 74.5% xylem sap, 15% kiwifruit puree and 10% high fructose. Nutritional facts in drink (252 mL) processed by using the xylem sap were 21.8 g sugar, 23.1 mg calcium, 14.1 mg potassium, 554.5 mg amino acid, and 15.6 mg ascorbic acid.

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Effects of different dietary ratio of metabolizable glucose and metabolizable protein on growth performance, rumen fermentation, blood biochemical indices and ruminal microbiota of 8 to 10-month-old dairy heifers

  • Sun, Jie;Xu, Jinhao;Ge, Rufang;Wang, Mengzhi;Yu, Lihuai;Wang, Hongrong
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.8
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    • pp.1205-1212
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    • 2018
  • Objective: The aim of this experiment was to evaluate the effects of different dietary ratio of metabolizable glucose (MG) to metabolizable protein (MP) on growth performance, blood metabolites, rumen fermentation parameters and the ruminal microbial community of 8 to 10-month-old heifers. Methods: A total of 24 Holstein heifers weighing an average of 282.90 kg (8 month of age) were randomly assigned to four groups of six. The heifers were fed one of four diets of different dietary MG/MP (0.97, 1.07, 1.13, and 1.26). Results: The results showed that the ratio of MG/MP affected the growth performance, blood metabolites, rumen fermentation parameters and the ruminal microbial community of heifers. The average daily gain of heifers was enhanced by increasing the ratio of MG/MP (p<0.05). The concentration of blood urea nitrogen, cholesterol, and low density lipoprotein cholesterol as well as the concentration of total volatile fatty acid in the rumen fluid of heifers decreased with the improvement in the ratio of dietary MG/MP (p<0.05). However, the relative amount of Ruminococcus albus and Butyrivibrio fibrisolvens in the rumen of heifers was increased significantly (p<0.05) when the dietary MG/MP increased. At the same time, with the improvement in dietary MG/MP, the amount of Fibrobacter succinogenes increased (p = 0.08). Conclusion: A diet with an optimal ratio (1.13) of MG/MP was beneficial for the improvement of growth, rumen fermentation, dietary protein and energy utilization of 8 to 10-month-old dairy heifers in this experiment.

Effects of Fine Powder Active Carbon Addition on the Wastewater Treatment Containing Phenol (Phenol함유 폐수의 처리에서 분말 활성탄 첨가의 영향)

  • 강선태;김정목
    • Journal of Environmental Health Sciences
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    • v.22 no.3
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    • pp.98-102
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    • 1996
  • This study investigated performance of the phenol degradation and reaction characteristics according to variation of phenol volumetric loading rates and dilution rates in suspension and PACT reactors using Pseudomonas sp. B3. 1. Removal efficiencies of the PAC unit indicated about 100 % with phenol volumetric loading rates from 0.4 phenol $kg/ma^3\cdot d$ to 1.2 phenol $kg/m^3\cdot d$, however, which of the suspension reactor showed about 100% with from 0.2 phenol $kg/m^3\cdot d$ to 0.75 phenol $kg/ma^3\cdot day$. 2. The cell density slightly was decreased from 298.2 mg/l to 272 mg/l, when dilution rate for suspension was reactor increased from 0.4 to 1.41 1/d, and also the cell density suddenly was decreased to 145.5 mg/l and was washed out at the dilution rate higher than 1.60 1/d. But the cell density for the PAC unit was linearly decreased with dilution rate of from 0.8 to 3.0 1/d, and showed 220.75 mg/l at maximum dilution rate. 3. The phenol utilization rate was increased from 0.008 to 0.031 phenol g/l$\cdot$h, when dilution rate for suspension reactor was increased from 0.4 to 1.5 1/d, however, the rate for the PAC unit was linearly increased from 0.017 to 0.061 phenol g/l$\cdot$h as variation changes from 0.017 to 0.061 phenol g/l$\cdot$h dilution rate.

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Physiocochemical Properties of Hovenia dulcis Thunb. Leaf Tea (헛개나무잎차의 이화학적 특성)

  • 정창호;배영일;심기환
    • Food Science and Preservation
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    • v.7 no.1
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    • pp.117-123
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    • 2000
  • In order to promote the utilization of Hovenia dulcis leaf as food, leaf tea was nanufactured and its physicochemical properties were examined. Aong proximate composiotns of Hovenia dulcis leaf tea were found in both fermented tea and roasted tea the higher contents of total sugar and crude protein. In minerals component of fermented tea and roasted tea, potassium (K) was the most high amounts of 11,560.6 ppm and 11,084.6 ppm, respectively. The highest contents of free sugar in both teas were mainly consisted of sucrose, the amounts of sucrose showed 1.47% and 1.94% . In case of organic acids, oxalic acid and citric acid were revealed 631.26mg% and 660.05mg%, respectively. The highest contents of total amino acid in fermented tea and roasted tea were 812.01mg% and 709.72mg% of glutamic acid. the fatty acids of fermented tea and roasted tea were mainly composed of 40.18% of linolenic acid and 26.92% of palmitic acid, respectively. The major volatile compounds of fermented tea and roasted tea were composed of 3, 7, 11, 15-tertramethyl-2-hexadecen-1-ol and methyl palmitate and the amounts of those showed 290.1ppm and 472.97 ppm, respectively. The tannins of fermented tea and roasted tea were 1.02% and 1.26% , as the extraction steps increased the tannin contents decreased. In Hunter's color values of tea extract L and b values decreased , while a value of those increased as the extraction steps were repeated.

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Antioxidative Activity of Solvent Fraction and Isolation of ANtioxidative Compound from Chestnut Husk (밤 귀피의 용매분획별 항산화 활성과 항산화 물질의 분리)

  • 권은정;김영찬;권미선;김창섭;강우원
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.4
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    • pp.726-731
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    • 2001
  • To enhance the utilization of chestnut husk discarded in the processing company antioxidative activities and compounds were investigated. Antioxidative activities of solvent fractions from chestnut husk were examined by benzoic acid hydroxylation method ferric thiocyanate method and DPPH test. Ethyl acetate fraction showed strong antioxidative activities comparable to BHA. Active compounds were isolated and purified from ethyl acetate fraction by Sephadex LH-20 column chromatography and preparative HPLC. A major active compound gallic acid was identified by $^{1}$H and $^{13}$ C-NMR. The phenolic acid contents was analyzed by GC and ellagic acid (172.22 mg%) and gallic acid (107.39 mg%) were major phenolic acid of chestnut husk.

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Utilization of Fly Ash as a Source of Mineral Fertilizers -IV. Development of Slowly Released K Fertilizer (Fly Ash 비료화(肥料化) 연구(硏究) -IV. Fly Ash를 이용(利用)한 지효성(遲效性) 가리(加里) 비료(肥料) 개발(開發))

  • Shin, Jae-Sung;Seong, Ki-Seog;Choi, Du-Hoi
    • Korean Journal of Soil Science and Fertilizer
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    • v.27 no.1
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    • pp.10-14
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    • 1994
  • The slowly released potassium fertilizer was developed by mixing fly ash with KOH, anthracite coal powder, KCI, $K_2CO_3$ and $Mg(OH)_2$ as the substances for accelerating calcination. Measuring proper ratios of raw materials, we found that the anthracite coal powder was 12 percent and $Mg(OH)_2$ was two to three percent. The optimal calcination temperature and time were proved to be $850^{\circ}C$ and 30 minutes, respectively, however, the trial product with lower temperature and shorter time in calcination had low quality. The K solubility of the product was very low compared to that of the KCl fertilizer.

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Removal of Aqueous Cyanide through the Utilization of Industrial By-products (산업부산물(제강 Sludge, 제강 Slag)을 이용한 시안 흡착 제거)

  • 이정원;현재혁;조재범
    • Journal of Korea Soil Environment Society
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    • v.3 no.2
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    • pp.89-99
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    • 1998
  • Sorption of the aqueous cyanide onto steel mill sludge and steel mill slag, both of which are the by-products from the converter furnace, was studied. In the study, the influence of temperature, activation energy, concentration and pH on sorption of cyanide was investigated. Three different temperature($25^{\circ}C$ > $37^{\circ}C$> $50^{\circ}C$) was chosen to represent that of landfill leachate. Initial concentration was 1 mg/$\ell$ 5 mg/$\ell$, 10 mg/$\ell$, and 20 mg/$\ell$. In addition, pH was set to three different level, that is, 3, 7, and 11 respectively. As the result of batch mode experiment for cyanide adsorption, the removal rate was found to be proportional to the initial concentration of cyanide. The order of removal rate was 20 mg/$\ell$> 10 mg/$\ell$> 5 mg/$\ell$> 1 mg/$\ell$. Similarly the influence of pH was proportional because of the change in solubility of cyanide. The order of removal rate was pH 11 > pH 7 > pH 3. As the temperature increased, so did the removal rate. The reaction was endothermic and the value of activation energy(Ea) was 127.93 J/mole and 59.44 J/mole respectively at 1 mg/ιand 20 mg/ιof initial concentration. From the experiment, it can be postulated that the capability of steel mill by-products to attenuate aqueous cyanide is enough to be used as substitute for clay liner of landfill site in the aspect of pollutant removal.

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Chemical Components of White and Red Lotus (목련과 홍련의 화학성분)

  • Jeong, Chang-Ho;Son, Ki-Bong;Kang, Seon-Gyeong;Shim, Ki-Hwan
    • Journal of agriculture & life science
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    • v.43 no.1
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    • pp.43-50
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    • 2009
  • To get basic data for the utilization of white and red lotus as a raw material in functional food, chemical components of its leaf and root were investigated. Leaf had the highest level of nitrogen free extract and root had the highest level of moisture. The mineral analysis showed K(380.44~1,516.34 mg/100g), Ca(36.67~1,323.92 mg/100g), P(84.02~473.13 mg/100g) and Na(57.73~304.07 mg/100g). The rich free sugars in white and red lotus were glucose(161.12~765.15 mg/100g) and frutose (100.57~901.12 mg/100g). Total amino acid contents in leaf and root of white lotus and leaf and root of red lotus were 6,385.57, 1,162.93, 6,003.01 and 1,242.20 mg/100g, respectively. Although the free amino acid compositions of white and red lotus were different, their major free amino acids were glutamic acid, phenylalanine arginine and tyrosine. The ascorbic acid were 248.65 and 20.99 mg/100g in leaf and root of white lotus, 156.92 and 9.32 mg/100g in leaf and root of red lotus. The leaf of white lotus exhibited the highest total phenolic contents at 24.33 mg/g.

Protective effect of Macleaya cordata isoquinoline alkaloids on lipopolysaccharide-induced liver injury in broilers

  • Jiaxin Chen;Weiren Yang;Hua Liu;Jiaxing Niu;Yang Liu;Qun Cheng
    • Animal Bioscience
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    • v.37 no.1
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    • pp.131-141
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    • 2024
  • Objective: This experiment aimed to explore the protective action of dietary supplementation with isoquinoline alkaloids (IA) from Macleaya cordata on lipopolysaccharide (LPS)-induced liver injury in broilers. Methods: Total 216 healthy broilers were selected in a 21-d trial and assigned randomly to the following 3 treatments: control (CON) group, LPS group, and LPS+IA group. The CON and LPS groups were provided with a basal diet, whereas the LPS+IA group received the basal diet supplemented with 0.6 mg/kg Macleaya cordata IA. Broilers in LPS and LPS+IA groups were intraperitoneally injected with LPS (1 mg/kg body weight) at 17, 19, and 21 days of age, while those in CON group were injected with equivalent amount of saline solution. Results: Results showed LPS injection caused systemic and liver inflammation in broilers, inhibited immune function, and ultimately lead to liver injury. By contrast, supplementation of IA ameliorated LPS-induced adverse change in serum parameters, boosted immunity in LPS+IA group. Furthermore, IA suppressed the elevation of hepatic inflammatory cytokines and caspases levels induced by LPS, as well as the expressions of genes related to the toll-like receptor 4 (TLR4)/myeloid differentiation primary response 88 (MyD88)/nuclear factor-kappa B (NF-κB) pathway. Conclusion: Dietary inclusion of 0.6 mg/kg Macleaya cordata IA could enhance immune function of body and inhibit liver damage via inactivating TLR4/MyD88/NF-κB signaling pathway in broilers.