• 제목/요약/키워드: Mg nanoparticles

검색결과 177건 처리시간 0.032초

Preparation of 5-fluorouracil-loaded Nanoparticles and Study of Interaction with Gastric Cancer Cells

  • Fan, Yu-Ling;Fan, Bing-Yu;Li, Qiang;Di, Hai-Xiao;Meng, Xiang-Yu;Ling, Na
    • Asian Pacific Journal of Cancer Prevention
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    • 제15권18호
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    • pp.7611-7615
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    • 2014
  • Aims: To prepare 5-fluorouracil (5-Fu) nanoparticles with higher encapsulation efficiency and drug loading, and then investigate interaction with the SGC-7901 gastric cancer cell line. Materials and Methods: Prescription was optimized by orthogonal experiments, the encapsulation efficiency and loading capacity were tested by high-performance liquid chromatography, and inhibition of proliferation by 5-Fu nanoparticles and 5-Fu given to cells for 24, 48 and 72 hours was investigated by methyl thiazolyl tetrazolium assay (MTT). In addition, 5-Fu nanoparticles were labeled by fluorescein isothiocyanate (FITC), and absorption into cells was tested by flow cytometry. Results: The optimal conditions for preparation were concentrations of 5-Fu of 5mg/ml, of $CaCl_2$ of 60 mg/ml and of chitosan of 2 mg/ml. With a stirring speed of 1200rpm, encapsulation efficiency of 5-Fu nanoparticles was $55.4{\pm}1.10%$ and loading capacity was $4.22{\pm}0.14%$; gastric cancer cells were significantly inhibited by 5-Fu nanoparticles in a time and concentration dependent manner, and compared to 5-Fu with slower drug release, in a certain concentration range, inhibition with 5-Fu nanoparticles was stronger. 5-Fu nanoparticles were absorbed by the cells in line with the concentration. Conclusions: 5-Fu nanoparticles can inhibit growth of gastric cancer cells in vitro to a greater extent than with 5-Fu with good adsorption characteristics, supporting feasibility as a carrier.

Preparation of Nanoparticles in Drug Delivery System Using Guar Derivatives and Dialysis Method

  • Na, Kun;Kim, Yu-Eun;Lee, Ki-Young
    • Journal of Microbiology and Biotechnology
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    • 제9권1호
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    • pp.50-55
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    • 1999
  • To develop a new form of controlled release dosage for administering for indomethacin (IND), two formulations of IND-loaded nanoparticles were designed based on polysaccharide (guar) derivatives. Nanoparticles prepared by the dialysis method were characterized with respect to morphology, size distribution, drug content, and in vitro drug release. Morphological studies by scanning electron microscopy (SEM) indicated that guar acetate (GA) nanoparticles were spherical in shape and had a smooth surface. The particle size distributions of formulation I (40mg of GA) and formulation II (80mg of GA) were shown to be $250.78\pm185.13nm$ and $718\pm145.90nm$ in distilled water ($20$^{\circ}C$), respectively. The drug loading efficiencies of nanoparticles were approximately 26% and 31% for formulations I and II, respectively. The differential scanning calorimetry (DSC) results indicated that the IND was perfectly distributed within GA nanoparticles. We also found, from the X-ray diffractometry analysis, that a decrease in the degree of crystallinity of the drug occurred in the nanoparticles. No changes between the original IND and the released IND from GA nanoparticles were detected by FT-IR. Using guar acetate, it is possible to design nanoparticles which allow the controlled release of IND over an extended period of time.

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음이온성 유화제로 수식된 폴리락티드/글리코리드 공중합체 나노 입자와 백신의 결합성 (Binding of Vaccine and Poly(DL-lactide-co-glycolide) Nanoparticle Modified with Anionic Surfactant)

  • 최민수;박은석;지상철;신병철
    • Journal of Pharmaceutical Investigation
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    • 제34권3호
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    • pp.177-183
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    • 2004
  • Recently, studies on intranasal mucosa delivery of influenza vaccine have been actively developed because of lack of pain and ease of administration. We studied on preparation of nanoparticle delivery system using biodegradable polymer as a poly(DL-lactide-co-glycolide) (PLGA) and their binding characteristics with vaccine. Three kinds of PLGA nanoparticles were prepared by spontaneous emulsification solvent diffusion (SESD) method using sodium dodecyl sulfate and sodium laurate as an anionic surfactant and Lutrol F68 (polyethylene glycol-block-polypropylene glycol copolymer) as a nonionic surfactant. The 5-aminofluorescein labeled vaccine was coated on the surface of nanoparticles by ionic complex. The complexes between vaccine and nanoparticles were confirmed by change of the size. After vaccine coating on the surface of anionic nanoparticles, particle size was increased from 174 to 1,040 nm. However the size of nonionic nanoparticles was not more increased than size of anionic nanoparticles. The amount of coated vaccine on the surface of PLGA nanoparticles was $14.32\;{\mu}g/mg$ with sodium dodecyl sulfate, $12.41\;{\mu}g/mg$ with sodium laurate, and $9.47{\mu}g/mg$ with Lutrol F68, respectively. In conclusion, prepared nanoparticles in this study is possible to use as a virus-like nanoparticles and it could be accept in the field of influenza vaccine delivery system.

은나노 소금과 은나노 설탕을 이용한 은나노 입자의 항균 특성 연구 (Antimicrobial Activity of Silver Nanoparticles Attached on the Surface of Salt and Sucrose)

  • 선용호
    • KSBB Journal
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    • 제28권4호
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    • pp.249-253
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    • 2013
  • In this study, we analyzed the attachment amount and the surface properties, such as shape and attachment aspect of silver nanoparticles on the surface of salt and sucrose. In addition, we investigated the antimicrobial activity of silver nanoparticles by measuring total colony counts and total coliforms in sewage according to the time and the amount of silver nanoparticles. As a result, it is found that silver nanoparticles are attached on the surface of salt and sucrose from the SEM images and there is no microorganisms on the surface of salt and sucrose. Silver nanoparticles on salt were rod shape but silver nanoparticles on sucrose were round shape. Also, the content of silver was 0.735 mg/g on salt and 0.885 mg/g on sucrose. In all experiments, total colony counts and total coliforms sharply declined initially, and it decreased gradually as change the time. When injection amount of nano silver sucrose and salt increased, the width of decline was greater. The amount of total colony counts and total coliforms of nano silver salt was much lower and the disinfection efficiency was higher comparing with nano silver sucrose. That means the case of nano silver salt is much better at the antimicrobial activity.

Screening of the antiparasitic activity of gold nanoparticles on hydatid cysts protoscolices in vitro

  • Sadiya Aziz Anah
    • 대한수의학회지
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    • 제63권4호
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    • pp.33.1-33.5
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    • 2023
  • Many scolicidal agents have been used to destroy fertile protoscolices, but these scolicidal agents have side effects, highlighting the need for research on effective and non-toxic replacement scolicidal agents. Gold nanoparticles (AuNPs) are biocompatible and non-toxic. The current study examined the effects of AuNPs in killing the protoscolices of Echinococcus granulosus in vitro using eosin staining. The protoscolices were treated with 0.2, 0.4, 0.8, or 1.0 mg/mL of AuNPs for 15, 30, 45, or 60 minutes. A concentration of 1.0 mg/mL was the most efficient in killing the protoscolices after 60 minutes exposure, reaching 96%, followed by 0.8 mg/mL (84.5%), whereas 0.4 and 0.2 mg/mL of AuNPs achieved a death rate of 76.8% and 68.5%, respectively. The loss of the protoscolices was lower at shorter exposure times with the same concentration of AuNPs and increased as the AuNP concentration was increased at the same exposure time. Significant differences were found between the different groups compared to the control group.

Phospholipid polymer can reduce cytotoxicity of poly (lactic acid) nanoparticles in a high-content screening assay

  • Kim, Hyung Il;Ishihara, Kazuhiko
    • Biomaterials and Biomechanics in Bioengineering
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    • 제1권2호
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    • pp.95-104
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    • 2014
  • The objective of this study was to evaluate the cytotoxicity of poly (lactic acid) (PLA) nanoparticles. We used a water-soluble, amphiphilic phospholipid polymer, poly (2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate) (PMB30W), as a stabilizer for the PLA nanoparticles. The PLA nanoparticles and PMB30W-modified PLA (PLA/PMB30W) nanoparticles were prepared by evaporating tetrahydrofuran (THF) from its aqueous solution. Precipitation of the polymers from the aqueous solution produced PLA and PLA/PMB30W nanoparticles with a size distribution of $0.4-0.5{\mu}m$. The partial coverage of PMB30W on the surface of the PLA/PMB30W nanoparticles was confirmed by X-ray photoelectron spectroscopy (XPS) and dynamic light-scattering (DLS). A high-content automated screening assay (240 random fields per group) revealed that the PLA nanoparticles induced apoptosis in a mouse macrophage-like cell line (apoptotic population: 73.9% in 0.8 mg PLA/mL), while the PLA/PMB30W nanoparticles remained relatively non-hazardous in vitro (apoptotic population: 13.8% in 0.8 mg PLA/mL). The reduction of the apoptotic population was attributed to the phosphorylcholine groups in the PMB30W bound to the surface of the nanoparticle. In conclusion, precipitation of PLA in THF aqueous solution enabled the preparation of PLA nanoparticles with similar shapes and size distribution but different surface characteristics. PMB30W was an effective stabilizer and surface modifier, which reduced the cytotoxicity of PLA nanoparticles by enabling their avoidance of the mononuclear phagocyte system.

Covalent Immobilization of Penicillin G Acylase onto Fe3O4@Chitosan Magnetic Nanoparticles

  • Ling, Xiao-Min;Wang, Xiang-Yu;Ma, Ping;Yang, Yi;Qin, Jie-Mei;Zhang, Xue-Jun;Zhang, Ye-Wang
    • Journal of Microbiology and Biotechnology
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    • 제26권5호
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    • pp.829-836
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    • 2016
  • Penicillin G acylase (PGA) was immobilized on magnetic Fe3O4@chitosan nanoparticles through the Schiff base reaction. The immobilization conditions were optimized as follows: enzyme/support 8.8 mg/g, pH 6.0, time 40 min, and temperature 25 ℃. Under these conditions, a high immobilization efficiency of 75% and a protein loading of 6.2 mg/g-support were obtained. Broader working pH and higher thermostability were achieved by the immobilization. In addition, the immobilized PGA retained 75% initial activity after ten cycles. Kinetic parameters Vmax and Km of the free and immobilized PGAs were determined as 0.113 mmol/min/mg-protein and 0.059 mmol/min/mg-protein, and 0.68 mM and 1.19 mM, respectively. Synthesis of amoxicillin with the immobilized PGA was carried out in 40% ethylene glycol at 25 ℃ and a conversion of 72% was obtained. These results showed that the immobilization of PGA onto magnetic chitosan nanoparticles is an efficient and simple way for preparation of stable PGA.

다중벽 탄소나노튜브의 수소 흡착 거동에 대한 Mg 나노입자의 영향 (Influence of Mg nanoparticles on Hydrogen Adsorption Behaviors of Multi-walled Carbon Nanotubes)

  • 유혜민;박수진
    • 한국신재생에너지학회:학술대회논문집
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    • 한국신재생에너지학회 2011년도 춘계학술대회 초록집
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    • pp.160-160
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    • 2011
  • In this work, magnesium (Mg) nanoparticles were plated onto the surfaces of multi-walled carbon nanotubes (MWNTs) in order to investigate the effects of their presence on the high pressure hydrogen storage behaviors of the resultant Mg/MWNTs. The structure of Mg/MWNTs was characterized by X-ray diffraction (XRD) and transmission electron microscopy (TEM). The pore structure and total pore volumes of Mg/MWNTs were analyzed by $N_2$/77 K adsorption isotherms. The hydrogen storage behaviors of the Mg/MWNTs were investigated by BEL-HP at 298K and 100 bar. From the results, it was found that Mg particles were homogeneously distributed on the MWNT surfaces. The hydrogen storage capacity increased in proportion to the Mg content. It can be concluded that Mg paricles play an important role in hydrogen storage characteristics due to the hydrogen spillover effect.

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Physical and nuclear shielding properties of newly synthesized magnesium oxide and zinc oxide nanoparticles

  • Rashad, M.;Tekin, H.O.;Zakaly, Hesham MH.;Pyshkina, Mariia;Issa, Shams A.M.;Susoy, G.
    • Nuclear Engineering and Technology
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    • 제52권9호
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    • pp.2078-2084
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    • 2020
  • Magnesium oxide (MgO) and Zinc oxide (ZnO) nanoparticles (NPs) have been successfully synthesized by solid-solid reaction method. The structural properties of ZnO and MgO NPs were studied using X-ray diffraction (XRD) and scanning electron microscopy (SEM). XRD results indicated a formation of pure MgO and ZnO NPs. The mean diameter values of the agglomerated particles were around to be 70 and 50 nm for MgO and ZnO NPs, respectively using SEM analysis. Further, a wide-range of nuclear radiation shielding investigation for gamma-ray and fast neutrons have been studied for Magnesium oxide (MgO) and Zinc oxide (ZnO) samples. FLUKA and Microshield codes have been employed for the determination of mass attenuation coefficients (μm) and transmission factors (TF) of Magnesium oxide (MgO) and Zinc oxide (ZnO) samples. The calculated values for mass attenuation coefficients (μm) were utilized to determine other vital shielding properties against gamma-ray radiation. Moreover, the results showed that Zinc oxide (ZnO) nanoparticles with the lowest diameter value as 50 nm had a satisfactory capacity in nuclear radiation shielding.

Effects of supplementation cysteine-coated Fe3O4 nanoparticles compared to FeSO4, on reproductive performance in male quail

  • Abdolvand, Esmail;Farzinpour, Amjad;Vaziry, Asaad
    • Advances in nano research
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    • 제9권1호
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    • pp.15-24
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    • 2020
  • Iron has a crucial role in growth as part of metalo-proteins like haemoglobin or myoglobin, enzymes; they are also involved in energetic reactions. Iron plays a vital role in fertility. At high doses, Iron has a harmful consequence on the reproductive system, which can be strongly reflected the final stage of spermatogenesis. Nutritional products are claiming to use nanotechnology and it is important to recognize the potential toxicity of nano-sized nutrients. Recently iron nanoparticles were proposed as a food additive for poultry. The objective of this study was to investigate the effects of L-cystein coated iron oxide nanoparticles on reproductive performance in male quails. The results of Fourier Transform Infrared Spectrometer, Alternating Gradient Force Magnetometer and Scaning Electron Microscopy showed that iron oxide nanoparticles was produced and have been coated with L-cycstein (Fe3O4-Cys NPs). A total of 100 one-week-old quail chicks were randomly placed to five groups of five replicates. Four quails (two male and two females) were raised in an individual cage for each replicate. The five experimental treatment diets consisted; negative control diet, with no Iron supplementation; positive control diet supplemented with 60 mg/kg of Fe3O4; treatment diets supplemented with 0.6, 6 and 60 mg/kg of L-cystein coated iron oxide nanoparticles. The hemoglobin, Red blood cell, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, gonadal somatic index, daily sperm production, total testicular sperm and sperm viability of the male quails that were fed with diet supplemented by 0.6 mg/kg of Fe3O4-Cys NPs were improved as compare with negative control. This study showed that not only the use of the Fe3O4-Cys nanoparticles had no side effects but also it can be used as a feed additive to improve the reproductive performance in male quails.