• 한국토양비료학회지
• /
• 제43권4호
• /
• pp.506-513
• /
• 2010

다양한 유기질비료 수준에서 Methylobacterium oryzae CBMB20의 처리가 고추의 생육촉진에 미치는 영향을 조사하였다. 유기질비료 권장시비수준의 70, 85, 100, 120% 시용구에서 시비량이 증가함에 따라 고추의 신장, 건물중 및 수량에서 유의성 있는 증가를 확인하였다. M. oryzae CBMB20의 접종 처리에서 고추의 생장이 증가하였으며, 고추의 수량 또한 각 유기질 비료 수준별로 10~35% 증가하였다. 수확기에 조사한 근권토양의 메탄올자화세균의 밀도는 M. oryzae CBMB20 처리구에서 2.51~2.63 log CFU $g^{-1}$ soil 이었으며, 미처리구에서는 1.0 log CFU $g^{-1}$ soil 이하로 나타났다. 따라서 접종한 M. oryzae CBMB20은 고추 작물 근권토양에 효과적으로 군집하였으며, 식물생장촉진 호르몬의 분비 등의 작용을 통한 작물생장촉진효과를 발휘한 것으로 판단된다. M. oryzae CBMB20을 접종하지 않은 경우에는 고추의 최대 생장과 수량이 유기질 비료 권장시비 수준 120%에서 나타났으나, M. oryzae CBMB20을 접종한 경우에는 최대 생장과 수량이 권장시비 수준에서 나타났다. 이러한 결과는 식물생장촉진미생물의 접종을 통하여 비료 사용량의 절감 가능성을 제시하는 것이며, 식물생장 촉진 미생물 제제로서 M. oryzae CBMB20의 활용이 가능하리라 사료된다.

• 한국토양비료학회지
• /
• 제43권4호
• /
• pp.514-521
• /
• 2010

지속가능한 친환경농업을 위해서는 비료와 함께 식물생장촉진 미생물 또는 생장조절물질을 적절히 혼합 사용하는 것이 바람직하다. 본 연구에서는 Methylobacterium oryzae CBMB20의 근권토양접종과 메탄올 엽면살포에 따른 고추의 생육촉진 효과를 유기질 비료의 시용 수준별로 조사하였다. M. oryzae CBMB20의 근권토양접종과 메탄올 엽면살포는 각기 고추생육을 증대시켰다. 또한 이들을 동시에 혼합 처리하였을 경우에는 고추의 생장촉진 효과가 더욱 현저하게 나타났다. M. oryzae CBMB20의 근권토양접종과 메탄올 엽면살포에 따른 고추 생장촉진 효과는 유기질 비료의 시용수준이 낮은 경우에 더욱 현저하게 나타났다. M. oryzae CBMB20와 메탄올을 혼합 처리한 경우 권장시비수준 100%와 300% 처리 사이에 고추생육과 수량에서 유의성 있는 차이가 없었다. 시비량이 지나치게 많을 경우 생장촉진제의 처리효과를 거둘 수 없으며, 적정한 시비 수준에서 M. oryzae CBMB20와 메탄올을 혼합 처리함으로써 고추를 비롯한 작물의 생장과 수량을 유지하면서도 시비량을 크게 줄일 수 있을 것으로 판단된다.

• 한국토양비료학회지
• /
• 제47권1호
• /
• pp.1-7
• /
• 2014

The objective of this study was to determine the effect of single and co-inoculation of plant growth promoting bacteria (PGPB) on early plant growth in Saemangeum reclaimed soil. Plant growth promoting Brevibacterium iodinum RS16 and Methylobacterium oryzae CBMB20 were inoculated on maize (Zea mays L.) and sorghum-sudangrass hybrid (Sorghum bicolor L.) grown in Saemangeum reclaimed soil. Single and co-inoculation of B. iodinum RS16 and M. oryzae CBMB20 increased plant height, dry biomass accumulation and macro-nutrient accumulation of maize and sorghum-sudangrass hybrid. M. oryzae CBMB20 treatment increased plant height in maize by 41.2% at 30 days after sowing (DAS), shoot dry weight and total dry weight compared to non-inoculated treatment. Macro-nutrient accumulation (N and P) in maize roots was significantly increased with co-inoculation treatment, K and Ca content was significantly increased at B. iodinum RS16 treatment compared to non-inoculated treatment. Macro-nutrient accumulation (P, K, Ca and Mg) in shoot was higher with M. oryzae CBMB20 treatment compared to non-inoculated treatment. In case of sorghum-sudangrass hybrid, co-inoculation treatment showed 33.7% increase in plant height compared to non-inoculated treatment at 30 DAS. M. oryzae CBMB20 treatment increased root dry weight and total dry weight, macro-nutrient accumulation in roots and N, Ca and Mg accumulation in shoot compared to non-inoculated treatment. P and K accumulation in shoot was significantly increased at co-inoculation treatment compared to non-inoculated treatment. This pot culture experiment demonstrated that single and co-inoculation of B. iodinum RS16 and M. oryzae CBMB20 increased the early growth and nutrient accumulation of maize and sorghum-sudangrass hybrid.

• Journal of Applied Biological Chemistry
• /
• 제54권2호
• /
• pp.120-125
• /
• 2011

In order to exploit Methylobacterium oryzae CBMB20 as of plant growth promoting agent, different inoculation methods have been evaluated. The present study aimed to evaluate soil, foliar, and soil+foliar inoculations of M. oryzae CBMB20 to improve the growth, fruit yield, and nutrient uptake of red pepper (Capsicum annuum L.) under greenhouse conditions. The population range of green fluorescent protein (gfp)-tagged M. oryzae CBMB20 using the three inoculation methods was 2.5-2.9 ${\log}_{10}$ cfu/g in the rhizosphere and 4.5-6.0 ${\log}_{10}$ cfu/g in the phyllosphere of red pepper plants. Confocal laser scanning microscopy results confirmed the colonization of M. oryzae CBMB20 endophytically on leaf surface. Plant height, fruit dry weight, and total biomass were significantly higher ($p{\leq}0.05$) in all M. oryzae CBMB20 inoculation methods as compared to non-inoculated control. Furthermore, uptake of mineral nutrients such as N, P, K, Ca, and Mg in red pepper plants in all M. oryzae CBMB20 inoculation methods was higher than in non-inoculated control. Comparative results of inoculation methods clearly demonstrated that soil+foliar inoculation of M. oryzae CBMB20 lead to the highest biomass accumulation and nutrient uptake which may be due to its efficient colonization in the red pepper rhizosphere and phyllosphere.

• 한국토양비료학회지
• /
• 제43권4호
• /
• pp.522-527
• /
• 2010

지베렐린산은 곰팡이 및 세균으로부터 생산되는 주요 식물생장촉진물질이며 다양한 산업에서 이용되고 있다. 본 연구는 Methylobacterium oryzae CBMB20을 이용하여 GA3를 생산하기 위해 탄소원 및 질소원을 선발하였으며, 선발된 탄소원 및 질소원의 최적 농도와 최적 비율을 조사하였다. 탄소원으로는 Na-succinate가 methanol, glucose, maltose, sucrose, fructose, lactose에 비해 가장 우수하였으며, 질소원으로는 $NH_4Cl$가 $NO_3$, $NaNO_3$, glycine 등에 비해 우수하였다. 배양액에서 Nasuccinate와 $NH_4Cl$를 각각 5 및 0.4 g $L^{-1}$ 농도 비율로 사용하였을 때 $CA_3$의 생산량이 가장 높았다. 또한 ammonium mineral salt 배지의 pH를 7로 유지하고 $30^{\circ}C$의 조건으로 96시간 배양하였을 때 $CA_3$의 생산이 최대로 나타나는 것을 확인하였다.

• 한국토양비료학회지
• /
• 제42권4호
• /
• pp.317-322
• /
• 2009

농업유용미생물을 이용한 원예 및 채소작물의 건전 유묘 생산을 위하여 본 연구에서는 growth pouch 실험을 통하여 6가지 식물생육촉진 미생물을 접종하여 작물의 초기 뿌리 생장에 미치는 영향을 살펴 보았다. 본 실험에 사용한 6가지 균주는 Methylobacterium oryzae CBMB20, Methylobacterium phyllosphaerae CBMB27, Methylobacterium suomiense CBMB120, Methylobacterium strains CBMB12, CBMB15와 CBMB17이었다. 대조구와 비교했을 CBMB12, CBMB17, 및 CBMB20접종은 상추 초기 뿌리 생육에 유의성있는 효과를 보였고, CBMB102접종은 배추 초기 뿌리 생육에 유의성있는 효과를, CBMB27접종은 토마토의 초기 뿌리 생육에 유의성있는 효과를 보였다. 또한 Methylobacterium suomiense CBMB120접종은 오이, 토마토, 배추, 그리고 Methylobacterium strain CBMB12접종은 고추의 뿌리 초기 생장을 크게 촉진시켰음을 알 수 있었다. 위의 결과를 통하여 실험한 균주들을 각각의 작물 육묘 포트에 처리할 때에도 유묘의 생장속도를 촉진 시킬수 있음을 예상할 수 있다.

• 한국토양비료학회지
• /
• 제45권1호
• /
• pp.51-58
• /
• 2012

Salinity is one of the most relevant abiotic factor limiting crop yield and its net primary productivity. In addition, salinity induces an increased stress ethylene synthesis in plants which, in turn, exacerbate the responses to the stressor. Bacterial single or co-inoculation effect was tested using previously characterized plant growth promoting (PGP) bacteria Brevibacterium iodinum RS16 and Methylobacterium oryzae CBMB20 on maize and sorghum-sudan grass hybrid under different concentrations of NaCl. Non-inoculated maize and sorghum-sudangrass hybrid showed 33.4% and 20.0% reduction in seed germination under highest NaCl (150 mM) level tested. However, under the same NaCl concentration, co-inoculation with B. iodinum RS16 and M. oryzae CBMB20 PGP strains increased the seed germination in maize (16.7%) and sorghum-sudangrass hybrid (4.4%). In Gnotobiotic growth pouch experiments conducted for maize and sorghum-sudangrass hybrid, co-inoculation of PGP B. iodinum RS16 and M. oryzae CBMB20 mitigated the salinity stress and promoted root length by 22.9% and 29.7%, respectively. Thus the results of this study could help in development of potential bioinoculants that may be suitable for crop production under saline conditions.

• 한국환경농학회지
• /
• 제30권2호
• /
• pp.223-228
• /
• 2011

BACKGROUND: Rhizosphere bacteria may improve plant growth and productivity both by supply nutrients and hormonal stimulation. Although many experiments have shown improvements in plant growth with inoculation of bacterial cultures to the rhizosphere, the main obstacle in the applications of plant growth promoting rhizobacteria in a large scale is the inconsistency of the results. We tested the growth promoting effects of Azospirillum and Methylobacterium strains on red pepper plant. METHODS AND RESULTS: Red pepper seedlings were grown for 25 days in a growth media inoculated with A. brasilense CW903 or M. oryzae CBMB20. The seedlings were transplanted and grown for 45 days in pots with soil in a greenhouse, at half the recommended level of fertilizer. Bacterial culture, $4.0{\times}10^9$ for A. brasilense CW903 and $5.8{\times}10^8$ CFU for M. oryzae CBMB20, was applied in root zone soil periodically every 10 days during the experiment. Inoculation of M. oryzae CBMB20 significantly increased the red pepper plant growth in terms of leaf number, height and mass of shoot, or root mass compared to uninoculated control plants. Although beneficial effects of A. brasilense on plant growth of many crops were observed, the growthpromoting effect of A. brasilense CW903 on red pepper plant was not found in this study. CONCLUSION(s): The factors responsible for the irregularities in plant growth promoting of rhizobacteria are difficult to elucidate. Extensive inoculation experiments in the greenhouse and in the field should enable us to define the factors critical to obtain successful application of plant growth promoting rhizobacteria.

• 한국토양비료학회지
• /
• 제45권1호
• /
• pp.59-65
• /
• 2012

Plant growth-promoting effects of rhizobacterial inoculation obtained in pot experiments cannot always be dependably reproduced in fields. In this study, we investigated the effect of inoculation with Azospirillum brasilense and Methylobacterium oryzae, which have displayed growth promoting effects in several pot experiments, on growth and fruit yield of red pepper under field condition in a plastic-film house. Four rows spaced 90 cm apart were prepared after application of compost ($10Mg\;ha^{-1}$), and red pepper seedlings (Capsicum annum L., Nocgwang) were transplanted in each row with 40-cm space. Experimental treatments were consisted of A. brasilense CW903 inoculation, M. oryzae CBMB20 inoculation, and uninoculated control. Twelve plots, 10 plants per plot, were allotted to the three treatments with four replicates in a completely randomized design. At the time of transplanting, 50 mL of each inoculum ($1{\times}10^8cells\;mL^{-1}$) was introduced into root zone soil of each plant, and re-inoculated at 7 and 14 days after transplant. Plant growth and fruit yield were measured during the experiment. Both A. brasilense CW903 and M. oryzae CBMB20 could not promote growth of red pepper plants. All growth parameters measured were not significantly different among treatments. There were large variations in fruit yield recorded on plot basis, and no statistically significant differences were found among treatments. The failure to demonstrate the expected plant growth promoting effect of the inoculants is possibly due to various environmental factors, including weather and soil characteristics, reducing the possibility to express the potential of the inoculated bacterial strains.

• 한국토양비료학회지
• /
• 제45권1호
• /
• pp.37-42
• /
• 2012

Improvement of plant growth by Methylotrophic bacteria can be influenced through alterations in growth modulating enzymes or hormones, especially by decreasing ethylene levels enzymatically by 1-aminocyclopropane-1-carboxylate (ACC) deaminase or by production of indole-3-acetic acid (IAA). In this study, the effect of seven strains of Methylobacterium on seedling ethylene emission of tomato and red pepper plants was evaluated under greenhouse condition. Ethylene emission was lowest in Methylobacterium oryzae CBMB20 inoculated tomato plants and CBMB110 inoculated red pepper plants at 47 days after sowing (DAS). However, at 58 DAS all inoculated plants showed almost similar pattern of ethylene emission. Methylobacterium inoculated tomato and red pepper plants showed significantly less ethylene emission compared to control. Our results demonstrated that Methylobacterium spp. inoculation promotes plant growth due to the reduction of ethylene emission and therefore can be potentially used in sustainable agriculture production systems.