• 한국균학회지
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• 제41권4호
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• pp.236-242
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• 2013

꽃송이버섯(Sparassis crispa)은 배양과정에서 항균물질인 sparassol, methyl orsellinate (ScI)와 methyl-dihydroxy-methoxy-methylbenzoate (ScII) 등의 화합물을 생산하는 것으로 알려져 있으며, 이 중 ScI와 ScII는 sparassol보다 높은 항균활성을 나타내는 것으로 보고되었다. 본 연구에서는 꽃송이버섯의 액체배양 시 균사체가 생산하는 항균 물질인 methy1 orsellinate와 sparassol을 분리하여 NMR과 ESI-MS 분석을 통해 그 구조를 동정하였으며, HPLC 분석을 통해 methy1 orsellinate와 sparassol의 retention time은 각각 15분과 31분임을 확인하였다. S. latifolia의 화합물 생산 양상은 크게 4가지로 대별되며, 균주에 따라 다르게 나타났다. 기주식물과의 상관관계를 분석한 결과, 일본잎갈나무와 잣나무에서 분리된 균주는 서로 다른 생산 양상을 보인 반면, 소나무와 전나무에서 분리된 균주는 동일한 생산 양상을 나타냈다. 균체량과 화합물 생산량과의 상관관계는 나타나지 않았다. Methy1 orsellinate 생산량은 낙엽송에서 분리된 KFRI 645 균주가 0.170 mg/ml로 가장 높았으며, sparassol 생산량은 소나무에서 분리된 KFRI 747 균주가 0.004 mg/ml로 가장 높았다. 이러한 결과를 통해 꽃송이버섯 균주들의 methyl orsellinate와 sparassol의 생산 양상은 기주식물의 종류에 따라 다르다는 사실을 확인하였다.

• 한국식품위생안전성학회지
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• 제12권3호
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• pp.195-199
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• 1997

For the purpose of isolation and screening of tyrosinase inhibitory activity from edible mushrooms, Pleurotus ostreatus, Auricularia auricula-Judae, Umbilicaria esculenta, Agaricus bisporus, Flammuline velutipes, Lentinus edodes, Ganoderma lucidum, and Coriouls versicolor were examined by tracing inhibitory activities against tyrosinase, utilizing L-3,4-dihydroxyphenylalanine (L-DOPA) as a substrate. Among the eight edible mushrooms tested, Umbilicaria esculenta showed potent enzyme inhibitory activities above 7804% against tyrosinase in ethylacetate (EtOAc) extracts. Ganoderma lucidum and Agaricus bisporus showed inhibitory activities of 67.3% and 51.5% in water extracts. EtOAc extracts of Umbilicaria esculenta was fractionated from silicagel column chromatography and one fraction showed the most inhibitory activity of 60.9%. The three bands (Rf=0.38, 0.27, 0.19) were isolated from preparative TLC of the fraction for purification and identified as mixtures of orsellinate, methyl orsellinate, methyl lecanorate, and methyl gyrophorate by high pressure liquid chromatography (HPLC), ultravisible spectrophotometer (UV), mass spectrophotometer (Mass), nuclear magnetic resonance spectrometer (NMR).

• 농업과학연구
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• 제47권1호
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• pp.183-191
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• 2020

Lichens are composite organisms consisting of a symbiotic association of a fungus with a photosynthetic partner (the photobiont or phycobiont), usually either a green alga or cyanobacterium. According to more recent studies, the biological activities of lichens and lichen substances include an antibiotic activity, antitumor and antimutagenic activity against human immunodeficiency virus (HIV), allergenic activity, plant growth inhibitory activity, and enzyme inhibitory activity. This study screened lichen extracts with a potent in vitro antifungal activity against plant diseases caused by phytopathogenic fungi. The compounds were isolated from Stereocaulon alpinum and Sphaerophorus globosus, and their chemical structures were identified as methyl hematommate, methyl β-orsellinate, 5-hydroxyferulic acid, sphaerophorin, and 2-heptyl-4,6-dimethoxybenzoic acid by electron ionization mass spectrometry (EI-MS) and nuclear magnetic resonance (NMR) spectral analyses. In vitro disease control against Alternaria mali, Cochliobolus miyabeanus, Colletotrium gloeosporioides, and Verticillum dahliae was evaluated. And among the five compounds, only methyl hematommate was effective against A. mali, C. miyabeanus, and C. gloeosporioides. The compounds were isolated from these lichens, which have a similar biosynthetic pathway, respectively. This is the first report of these compounds being isolated from these lichens.