• Asian-Australasian Journal of Animal Sciences
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• v.29 no.5
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• pp.689-694
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• 2016

The objective of the current study was to evaluate the effect of supplementation of either L-methionine (L-Met) or DL-methionine (DL-Met) to diets of starter pigs on nitrogen (N) balance, metabolism, and serum amino acid profile. Eighteen crossbred ($Duroc{\times}Landrace{\times}Yorkshire$) barrows weighing $15.45{\pm}0.88kg$ were randomly allotted to 1 of 3 diets with 6 pigs per treatment. The diets included a basal diet (Met-deficient diet) containing 0.24% standardized ileal digestibility Met with all other essential nutrients meeting the pig's requirements. The other two diets were produced by supplementing the basal diet with 0.12% DL-Met or L-Met. The experiment lasted for 18 days, consisting of a 13-day adaptation period to the diets followed by a 5-day experimental period. Pigs were fed ad libitum and free access to water throughout the experiment. Results showed that the supplementation of either L-Met or DL-Met improved N retention, and serum methionine concentration, and decreased N excretion compared with basal diet (p<0.01). The N retention of pigs fed diets supplemented with the same inclusion levels of DL-Met or L-Met were not different (p>0.05). In conclusion, on equimolar basis DL-Met and L-Met are equally bioavailable as Met sources for starter pigs.

• Asian-Australasian Journal of Animal Sciences
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• v.8 no.4
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• pp.403-409
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• 1995

A growth experiment was conducted to evaluate the nutritivie values of supplemental lysine and methionine in broiler chicks. Two types of L-lysine, liquid and powder type, and DL-methionine were added to the diets at different levels of dietary protein with two growth phases, 0-3 weeks and 4-6 weeks named starter and grower, respectively. Six hundred seventy two chicks were allotted in 14 treatments; 3 controls by dietary CP level (starter-grower) with CP 23-21%, CP 21-19% and CP 20-18, 8 groups of liquid and powder lysine supplementation of 0.1, 0.2, 0.3 and 0.4%, and 3 groups of lysine and methionine supplementation. Body weight, feed intake, and excreta were measured and analyzed to determine growth performance, amino acid digestibilities, and the quantity of excreted nitrogen in feces. Chicks fed CP 23-20 with 3,200 ME kcal showed significantly better growth performance than those fed CP 21-18 for 6 weeks. The supplementation of 0.2% of either type of lysine to CP 21-19 diet improved weight gain and feed efficiecy to the extent that CP 23-21 diet was fed. Physical type of lysine did not affect chick's growth and amino acid digestibilities of the diets. The level of CP in the diet significantly affected nitrogen excretion in feces. Supplementation of lysine and methionine to CP 21-18 diet reduced fecal nitrogen by 10% compared to CP 23-21 diet. It was confirmed that 0.2% of supplemental lysine to the broiler diet spared the dietary protein by 3%, and also reduced nitrogen excretion in feces by 10%.

• Journal of Fisheries and Marine Sciences Education
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• v.29 no.3
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• pp.888-898
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• 2017

Tenebrio molitor larvae, also known as yellow mealworms (MW), are rich in protein and lipid and can serve as a potential alternative protein and energy source in commercial aquafeeds. Therefore, this study attempts to evaluate the effects of different drying methods on the nutritional value of MW meal. For this, live MW were cold-anaesthetized before being subjected to three different types of drying methods, including freeze-drying, oven-drying at $60^{\circ}C$ and air-drying at room temperature for three days, and compared for proximate composition and energy content. An in-vivo digestibility test was then conducted to evaluate the nutrient digestibility of MW meal in diets for rockfish, Sebastes schlegeli. A test diet was prepared by mixing the MW meal with a reference diet (Ref) in a 30:70 ratio with chromium oxide as an inert marker at the inclusion level of 0.5%. Rockfish with mean body weight of 150 g were stocked into a fecal collection system equipped with fiberglass tanks of 400 L capacity. Each group of fish was fed one of the experimental diets to apparent satiation for 4 weeks. The results of the proximate analysis showed that drying methods had no significant effect on crude protein, crude lipid, ash and energy contents of MW. Despite being a rich source of protein and lipid, MW meal was deficient in certain amino acids, particularly methionine, and highly unsaturated fatty acids, particularly 22:6n-3 (DHA) and 20:5n-3 (EPA). MW meal showed high digestibility values for protein (93%), lipid (97%) and energy (88%). These results may indicate that MW meal is a nutritious and acceptable feed ingredient, with comparable digestibility values to conventional animal and plant feedstuffs such as fish meal and soybean meal, in practical diet for rockfish at grower stage.

• Korean Journal of Organic Agriculture
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• v.9 no.1
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• pp.51-60
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• 2001

An experiment was carried out to evaluate the effects of adding phytase on nutrient digestibility in growing pigs. Three barrow pigs averaging 28.5$\pm$3.1kg of body weight were allotted to three treatments by 3$\times$3 latin square. Treatments included 1) com-soybean meal based-control diet and 2) and 3) control diet with phytase 500 and 1,000 unit. There were no effects of treatments on dry matter and nitrogen digestibility (P〉0.05). Ash, Ca and P digestibility in pigs fed diet with phytase were greater than those in pigs fed control diet(P〈0.05). An increased phosphorus intake was observed from pigs fed diet with treatments compared to that from pigs fed control diet. Pigs fed diet with adding phytase had improved P absorption compared to pigs fed control diet(P〈0.05). There was no significantly effect on fecal and urine excretion(P〈0.05). Ca intake was increased for pigs fed adding phytase compared to pigs fed control diet. No evident effect was observed on Ca fecal excretion among dietary treatments, however, urine excretion of pigs fed diet with treatments was decreased compared to pigs fed control diet. Cystine, isoleucine, tyrosine and phenylalanine digestibility of amino acid were greater for pigs fed diet with treatments than those for pigs fed control diet(P〈0.05). Asparagine, threonine, serine, methionine and leucine digestibility were improved for pigs fed with phytase 500 unit compared to pigs fed control diet. Lysine, histidine, arginine and other animo acids digestibility were higher for pigs fed diet with phytase than control diet, but there were no significant effects by the treatments(P〉0.05). In conclusion, the results of the experiments suggest that phytase supplementation in the diets could be a very beneficial strategy in environmental aspects.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.7
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• pp.1100-1107
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• 2007

The effects of offering broilers phosphorus-adequate diets containing 10.0 and 11.8 g/kg lysine, without and with 500 FTU/kg exogenous phytase, on growth performance and nutrient utilisation were determined. Each of the four experimental diets was offered to 6 replicates of 10 birds from 7 to 28 days of age. Effects of treatment on performance, apparent metabolisable energy, apparent ileal digestibility of amino acids and bone mineralisation were examined. Both additional lysine and phytase supplementation improved (p<0.05) weight gain and feed efficiency, with interactions (p<0.05), as phytase responses were more pronounced in lysine-deficient diets. Phytase improved (p<0.05) apparent metabolisable energy, which was independent of the dietary lysine status. Bone mineralisation, as determined by percentage toe ash, was not affected by treatment, which confirms the phosphorus-adequate status of the diets. Phytase increased (p<0.05) the apparent ileal digestibility of the sixteen amino acids assessed. Unexpectedly, however, the dietary addition of 1.8 g/kg lysine, as lysine monohydrochloride, increased (p<0.05) the ileal digestibility of lysine per se and also that of isoleucine, methionine, phenylalanine, valine, aspartic acid, glutamic acid and tyrosine. In addition, there were significant interactions (p<0.05) between additional lysine and phytase supplementation for arginine, lysine, phenylalanine, aspartic acid, glutamic acid, glycine and serine digestibilities, with the effects of phytase being more pronounced in lysine-deficient diets. The possible mechanisms underlying the increases in amino acid digestibility in response to additional lysine and the interactions between lysine and microbial phytase in this regard are discussed. Also, consideration is given to the way in which phytate and phytase may influence ileal digestibility of amino acids.

• Journal of Animal Science and Technology
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• v.64 no.4
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• pp.740-751
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• 2022

Two experiments were conducted to determine the effects of bird age on apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of amino acids (AA) for 10-d-old Experiment (Exp. 1) and 22-d-old (Exp. 2) male broilers. This study investigated the effects of different broiler ages and feed ingredients on AID and SID of AA in corn and soybean meal (SBM). Four hundred and eighty (age = 7 d; initial body weight [BW] = 173.4 ± 12.65 g) and 192 (age = 18 d; initial BW = 772.2 ± 62.13 g) birds were allocated to three dietary treatments in a randomized complete block design with eight replicate cages per treatment. Two diets were formulated based on corn or SBM as the sole source of AA in the diet. A nitrogen-free diet was also formulated to measure basal endogenous losses of AA. Experimental diets were given for 3 and 4 days in Exps. 1 and 2, respectively. An interaction was observed (p < 0.05) between the age of birds and the type of ingredient for the AID of most AA, except for methionine, valine, cysteine (Cys), and tyrosine; however, the effects of age and type of ingredients were diminished in the SID of AA, except for histidine, isoleucine, leucine (Leu), phenylalanine, alanine (Ala), and glutamic acid (Glu). The AID of AA, except for Leu and Cys and the SID of AA, except for Leu, Ala, Glu, and Pro in SBM were greater (p < 0.05) than in corn. As the age of birds increased from 10 to 22 d, digestibility of all AA increased (p < 0.05), regardless of the expression of AA digestibility (i.e., AID and SID). In conclusion, the AID and SID of AA in both corn and SBM increased with increasing age, and the AID and SID of AA in SBM were greater than in corn.

• Journal of Animal Science and Technology
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• v.44 no.4
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• pp.399-406
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• 2002

An experiment was conducted to investigate the effects of supplemental copper or/and zinc methionine chelates(Cu-Met or/and Zn-Met) on the performance, nutrient digestibility, serum IgG level, gizzard erosion, and the contents of Cu and Zn in liver and excreta of broiler chickens. One thousand d-old broiler chickens (Ross$^{(R)}$) were assigned to 4 treatments: control, 100 ppm Cu in methionine chelate(Cu-Met), 100 ppm Zn in methionine chelate(Zn-Met) and 100 ppm Cu plus 100 ppm Zn in methionine chelate(Cu-Zn-Met). Each treatment had five replications of 50 (25 male + 25 female) birds each. Average weight gains of chicks fed chelated Cu or/and Zn were significantly higher than that of chicks fed the control (P<0.05). Moreover, feed conversion rates of chicks were better in the chicks fed chelated Cu or/and Zn than in the chicks fed the control (P<0.05). The birds fed the chelated Cu and Zn(Cu-Zn-Met) tended to perform the best growth rate and feed conversion rate. Nutrient digestibilities were not affected by the dietary treatments. Serum IgG level of chicks fed Cu-Zn-Met was significantly higher than that of chicks fed the control (P<0.05). Gizzard erosion index was not significantly different among the treatments. The contents of Cu and Zn in liver were not significantly affected by the dietary treatments. The excreta contents of Cu or/and Zn were significantly high in the birds fed supplementary Cu or/and Zn. It was concluded that dietary supplementation of Cu or/and Zn in methionine chelated form improved growth and feed conversion efficiency of broilers.

• Asian-Australasian Journal of Animal Sciences
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• v.3 no.4
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• pp.353-361
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• 1990

A number of correlation and regression analyses were performed on data from apparent and true digestibility of amino acids at ileal and fecal level with finishing pigs, in order to investigate whether these amino acid digestibilities could be predicted with certainly degree on their fiber fractions content (chemical analysis). The data comprised 16 diets varying in 4 levels of crude fiber and from 4 fiber sources. The relationships between fiber fractions including crude fiber (CF), NDF, ADF lignin and cellulose contents on apparent and true digestibility of almost all amino acids in both ileal and fecal level were negative, except glutamine at fecal level. In apparent digestibility at ileal level, the correlations of fiber fractions were moderate (r of NDF = 0.53 to 0.63; ADF, 0.50 to 0.77; cellulose, 0.50 to 0.75), with an exception of CF content was relatively high (r of 0.58 to 0.81). The correlations to true digestibility of amino acids were weaker. In case of at fecal level, the higher correlation (negative) was found with NDF than CF content. Estimations of amino acids digestibility were performed using regression equation. The data showed that apparent digestibility of amino acids could be estimated for almost amino acids except arginine, threonine, valine and tyrosine at fecal level and phenylalaine, valine and glycine at ileal level. The best prediction at ileal and fecal level ($r^2=0.55-0.77$ and 0.52-0.76), respectively was obtained with NDF content. Prediction for true digestibility of amino acids, none of fiber fractions could be estimated for arginine, leucine and valine at all collection levels. At ileal level, CF could be used for most of amino acids except phenylalanine, glycine and praline; cellulose, only for lysine and methionine and NDF, only for proline. At fecal level, glutamine digestibility could be estimated only from CF and ADF content. The best predictor at ileal level was ADF content whereas at fecal level was NDF content. These results indicate that lignin content could not be used as predictor for all amino acids at both levels neither in apparent nor true digestibility.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.10
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• pp.1461-1476
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• 2009

This review focuses on the metabolic and osmoregulatory functions of betaine and its impact on nutrient digestibility and performance in pigs and poultry. Betaine is the trimethyl derivative of the amino acid glycine, and is present in plant and animal tissue. It has been shown to play an important role in osmoregulation of plants, bacteria and marine organisms. Due to its chemical structure, betaine exerts a number of functions both at the gastrointestinal and metabolic level. As a methyl group donor, betaine is involved in transmethylation reactions and donates its labile methyl group for the synthesis of several metabolically active substances such as creatine and carnitine. Therefore, supplementation of betaine may reduce the requirement for other methyl group donors such as methionine and choline. Beneficial effects on intestinal cells and intestinal microbes have been reported following betaine supplementation to diets for pigs and poultry, which have been attributed to the osmotic properties of betaine. Furthermore, betaine potentially enhances the digestibility of specific nutrients, in particular fiber and minerals. Moreover, at the metabolic level, betaine is involved in protein and energy metabolism. Growth trials revealed positive effects of supplemental betaine on growth performance in pigs and poultry, and there is evidence that betaine acts as a carcass modifier by reducing the carcass fat content. In conclusion, due to its various metabolic and osmoregulatory functions, betaine plays an important role in the nutrition of monogastric animals.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.5
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• pp.667-670
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• 2005

The proximate analysis, amino acid content and true amino acid digestibility and TMEn for poultry of adult Field crickets Gryllus testaceus Walker, were investigated. The insect was also used as partial replacement of protein supplements in the broiler diet on an equal CP percentage and TMEn basis. The results indicated that the adult insect contained: crude protein 58.3%; fat 10.3%, chitin 8.7% and ash 2.96% on dry matter basis, respectively. The total amounts of methionine, cystine and lysine in the Field crickets were 1.93%, 1.01% and 4.79%, respectively, and their true digestibility coefficients, determined in cecectomized roosters, were 94.1%, 85% and 96%, respectively. The TMEn of this insect meal was 2,960 kcal/kg determined in cecectomized roosters. When cornsoybean meal diets were formulated on an equal CP percentage and TMEn basis, up to 15% Field cricket could replace control diet without any adverse affects on broiler weight gain, feed intake or gain:feed ratio from 8 to 20 d posthatching.