• 한국동물학회:학술대회논문집
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• 한국동물학회 1996년도 공동 춘계학술대회
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• pp.58.1-58
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• 1996

No Abstract, See Full Text

• Parasites, Hosts and Diseases
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• 제45권4호
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• pp.295-299
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• 2007

The purpose of this study was to investigate whether sporulated Neospora caninum oocysts, which had been stored for 46 mo in a 2% sulfuric acid solution at $4^{\circ}C$, remain morphologically viable and infective to gerbils (Meriones unguiculatus). Six gerbils were orally inoculated with doses of 400 or 1,200 oocysts. Two mo after inoculation, the animals did not show any clinical signs, had no histological lesions, and were seronegative for N. caninum at 1:50 in an immunofluorescent antibody test. PCR using the brain from each gerbil did not reveal N. caninum specific DNA. We conclude that oocysts preserved for 46 mo are not infective, despite being morphologically intact.

• 대한수의학회지
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• 제47권4호
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• pp.363-370
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• 2007

Mongolian gerbil (Meriones unguiculatus) has been as an model animal for studing the neurological disease such as stroke and epilepsy because of the congenital incompleteries in Willis circle, as well as the investigation of water metabolism because of the long time-survival in the condition of water-deprived desert condition, compared with other species animal. Aquaporin 2 (AQP2) expressed at the surface of principal cells in collecting duct results from an equilibrium between the AQP2 in intracellular vesicles and the AQP2 on the plasma membrane. Aquaporin 4 (AQP4), which is expressed in cell in a wide range of organ, is also present in the collecting duct principal cells where this is abundant in the basolateral plasma membranes and represent potential exit pathways from the cell for water entering via AQP2. In this research, we divide 3 groups of which each group include the 5 animals. In the study of 7 or 14 days water restricted condition, we investigated the AQP2 and AQP4 by using a quantitative immunohistochemistry in the kidney. The results obtained in this study were summarized as followings. AQP2 is abundant in the apical plasma membrane and apical vesicles in the collecting duct principal cell and at rare abundance in connecting tubules. In the water-deprived Mongolian gerbil kidney, expression of AQP2 was continuosly increased in the cortical collecting duct and inner medullary collecting duct. This increase was both the apical region and cytoplasm. AQP4 is mainly expressed in the inner medulla, although some expression is also noted in the more proximal segment. In the water-deprived Mongolian gerbil kidney, AQP4 was also increased in the inner medullary collecting duct. Immunoactivity was increased in entire inner medullary collecting duct and newly detected in cytoplasm of principal cell. These findings suggest that increased levels of AQP2 and AQP4 in the cortical and inner medulalry collecting duct may play a important role for maintain fluid balance in the water-deprived kidney.

• 대한수의학회지
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• 제40권4호
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• pp.653-660
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• 2000

몽골리안 저빌(Meriones unguiculatus)의 위장관을 분문부, 기저부, 유문부, 십이지장, 공장, 회장, 맹장, 결장 및 직장의 9부분으로 구분하고 이들 부위에서 cholecystokinin(CCK)-8, gastrin, secretin, pancreatic polypeptide(PP), somatostatin, serotonin, glucagon 및 insulin 등 8종의 항혈청에 대한 내분비 세포의 부위별 분포 및 출현빈도를 면역조직화화적으로 관찰하였던 바, CCK-8, gastrin, somatostatin 및 serotonin 면역반응세포들이 관찰되었다. 이들 면역반응세포들은 위 부위에서는 원형에서 타원형으로 주로 위샘(gastric gland) 또는 주세포와 벽세포 사이에서 관찰되었고, 장 부위에서는 원형 또는 타원형의 면역반응세포들이 공장의 장샘(intestinal gland)에서 관찰되었으며, 타원형에서 방추형의 세포들이 장상피세포 사이에서 관찰되었다. CCK-8 면역반응세포들은 유문부와 십이지장에 국한되어 다수 또는 소수 관찰되었으며, gastrin 면역반응세포들은 유문부에 국한되어 다수 관찰되었다. Somatostatin 면역반응세포들 역시 gastrin 변역반응세포들과 유사하게 유문부에 국한되어 중등도의 출현빈도를 나타내었고, serotonin 면역반응세포들은 분문부와 맹장을 제외한 위장관 전부위에 걸쳐 중등도 또는 다수 관찰되었으나, secretin, PP, glucagon 및 insulin 면역반응세포들은 관찰되지 않았다. 이상에서 몽골리안 저빌의 위장관에 존재하는 내분비 세포들의 종류 및 출현빈도는 다른 설치류에 비해 매우 적거나 낮은 것으로 관찰되었으며, 이들 내분비 세포들의 출현부위 역시 다른 설치류에서와는 달리 일부 부분에 국한되는 것으로 관찰되었다.

• Parasites, Hosts and Diseases
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• 제40권4호
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• pp.181-186
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• 2002

To examine the fate of Strongyloides venezuelensis, Mongolian gerbils (Meriones unguicalatus) were orally infected with 1,000 $L_{3}$ larvae per animal. Altogether, 50 gerbils divided into 5 groups of 10 each were monitored for a period of 570 days to document the kinetics of faecal egg output, adults worm population, morphological development, fecundity, and hematological changes including peripheral blood eosinophilia. This study chronicled a life long parasitism of S. venezuelensis in the gerbil host, and showed that S. venezuelensis infection was quite stable throughout the course of infection and the worms maintained their normal development as evidenced by their body dimension. A progressive loss of body condition of the infected gerbils was observed as the level of infection advanced. However, no detectable pathological changes were observed in the gastrointestinal tract. The present findings indicate that an immunocompetent host, such as the Mongolian gerbil, can serve as a life long carrier model of S. venezuelensis if the worms are not expelled within 570 days after infection.

• Applied Microscopy
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• 제27권4호
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• pp.433-452
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• 1997

Mongolian gerbil (Meriones unguiculatus) has been as an animal model for studing the neurological diseases such as stroke and epilepsy because of the congenital incompleteries in Willis circle, as well as the investigation of water metabolism because of the long time-survival in the condition of water-deprived desert condition, compared with other species animals. In order to accomplish the this research, first of all another divided the laboratory animals 5 groups of which each group include the 5 animals. In this study were investigated the histological structure in the kidney, measured the plasma osmolalities at the time of sacrifice of indivisual animals, and the body weights every day during water-deprived. The results obtained in this study were summarized as followings: 1. The body weights and decreasing rates of the body weight in water-deprived mongolian gerbil groups were continuosly decreased. 2. The plasma osmolalities were increased from the 5th water-deprived day, after then the gradually increasing reached nearly its equilibrium state at the 10th water-deprived day. 3. The urine volumes were abruptly decreased from the 2th water-deprived day, after then the gradually decreasing patterns were reached nearly its equilibrium state at the 10th day, and stopped the 11th day. 4. In the light microscopical observation of the kidney, glomerular capillary loop thickening, mesangial matrix increasing, sclerosis, glomerular cystic atrophy, interstitial fibrosis, tubular dilatation, mononuclear interstitial inflammation, interstitial mineralization, and hyperplasia of the collecting duct epithelium in the cortex area, were observed from the 10th water deprived day, and the lesions were gradually severe changed as the time lapse. 5. In the electron microscopical findings of the kidney, the degenerative changes of endothelial cell, podocyte and mesangial cell in glomeruli were initially observed on the 10th water-deprived day as well as the degeneration of microvilli and intracellular organelle in the renal tubules.

• 대한수의학회지
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• 제52권2호
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• pp.105-113
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• 2012

In this study, a medicinal herbal plant, Meliae fructus, was examined and screened for anti-Helicobacter (H.) pylori activity. Seventy percent ethanol was used for herbal extraction. For anti-H. pylori activity screening, inhibitory zone tests as an in vitro assay and in vivo study using a Mongolian gerbil (Meriones unguiculatus) model were performed. Also, the safety of herbal compounds was evaluated by animal study. As a result of inhibitory zone test, Meliae fructus extract demonstrated strong anti-H. pylori activities. Also, as results of in vivo animal studies, Meliae fructus demonstrated strong therapeutic effects against H. pylori infection according to the criteria of histological examination and rapid urease test. As results of the safety study, after 28 days treatment of the Meliae fructus extract, the animals were not detected any grossly and histological changes. These results demonstrate that it can be successfully cured against H. pylori infection and protected from H. pylori-induced pathology with Meliae fructus. It could be a promising native herbal treatment for patients with gastric complaints including gastric ulcer caused by H. pylori.

• 대한수의학회지
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• 제40권2호
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• pp.257-265
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• 2000

Harderian glands are the unique organs in several mammals, which human and non-human primates do not have. We report the ultrastructural changes in the postnatal developmental periods of Harderian glands in Mongolian gerbil(Meriones unguiculatus). Male and female Mongolian gerbils were sacrificed on days 3, 10, 30 and 60 after birth and their Harderian glands were observed by transmission electron microscope. The obtained results were summarized as follows; 1. In 3-day-old Mongolian gerbils, Harderian gland was composed of one excretory duct and immature tubules which have two type cells, dark and light cells, identified electron-dense and electron-lucent respectively. 2. In 10-day-old Mongolian gerbils, small lipid vacuoles began to be found in the cytoplasm of the secretory cells of the Harderian gland. Mitochondria, Golgi apparatus, polysomes and slash were more abundant in the cytoplasm of dark cells than those of light cells. The arrangement of tubules in the gland was much more condensed than that of 3-day-old Mongolian gerbils. 3. In 30-day-old Mongolian gerbils, the secretory cells of the tubule were typically columnar in shape and there was one type cell in the tubule. Most of the columnar secretory cells contained various size vacuoles. 4. In 60-day-old Mongolian gerbils, the Harderian gland possessed the typical structural characteristics of adults. The mature glandular structures were more significant than those of 30-day-old animals.

• Applied Microscopy
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• 제37권4호
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• pp.271-280
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• 2007

Tonicity responsive enhancer binding protein(TonEBP)는 콩팥에서 osmolyte의 세포내 축적을 촉매해 주는 전사조절인자로 높은 삼투농도에서 세포를 보호하는데 중요한 역할을 수행한다. 고장성환경은 TonEBP의 양적 증가와 핵 내 분포의 증가를 통해 TonEBP의 활성을 자극한다. 또한 TonEBP는 콩팥 수질내 요소축적에 중요한 역할을 하는 UT-A의 전사를 조절하는 것으로도 알려져 있다. 따라서 본 연구에서는 콩팥수질내 TonEBP와 UT-A의 기능과 상관관계를 밝히는 연구의 일환으로 다른 동물보다 급수가 제한된 환경에서 더 오래 살아남을 수 있는 수분대사능력을 가지고 있는 Mongolian gerbil을 이용하여, 절수로 인한 고장성환경의 유발에서 TonEBP와 UT-A에 대한 발현 변화를 관찰하고자 하였다. 절수에 따른 TonEBP와 UT-A의 발현 양상을 연구하기 위해, 먼저 Mongoian gerbil 각 5마리씩 3그룹으로 나누어 절수 실험을 실시하였고, 면역조직화학법을 실시하여 다음과 같은 결과를 얻었다. 정상대조군에서 TonEBP의 면역반응성은 속수질 세포들의 핵 내에 주로 분포하였으며, 절수 7일군에서 면역조직화학검사 결과, 속수질집합관에서의 염색성은 대조군에 비해 증가하였고, 특히 바깥수질 부위에 속수질에서 요세관의 가는 부분에서의 증가가 두드러졌다. 절수 14일군에서 염색성이 대조군보다 오히려 감소하였으며, 콩팥의 조직학적 손상이 관찰되었다. UT-A의 경우 바깥수질 속줄무늬층의 짧은-헨레고리가는내림부분과 정상군에서는 미약한 양성반응을 나타낸 속수질 초기부분의 긴-헨레고리가는내림부분에서 강한 발현 양상을 나타내었고, 속수질의 초기에서 중간부위의 속수질집합관도 강한 발현 양상을 확인할 수 있었다. 그러나 속수질 말단부위의 속수질집합관은 콩팥유두 끝으로 갈수록 발현량이 감소하는 것을 확인할 수 있었다. 이상의 결과는 Mongolian gerbil을 이용한 절수모델에서 증가된 콩팥수질의 Tonicity에 의해 TonEBP의 발현이 증가하고 이에 따라 UT-A의 발현도 동반하여 증가하는 것을 확인하였고, 또한 이렇게 증가된 TonEBP는 UT의 전사를 조절하여 UT를 증가시켜 오줌농축기전을 향상시키는 것으로 생각된다. 이는 속수질 세포의 스트레스에 대한 세포방어기전으로 생각된다. 그러나 절수가 계속되면 이런 적응반응에 한계를 지나쳐 오히려 TonEBP와 UT-A의 발현이 감소함을 확인 할 수 있었다.

• 대한수의학회지
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• 제39권1호
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• pp.1-11
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• 1999

This investigation was carried out to study morphological and chronological aspects of the development of the Harderian gland in the Mongolian gerbil(Meriones unguiculatus). Male and female Mongolian gerbils were sacrificed on days 1, 3, 5, 10, 30 and 60 after birth and their Harderian glands were processed for light microscopic observation. The results obtained were summarized as follows; 1. In 1-day-old Mongolian gerbil, Harderian gland was well distinguished from other tissue structures. It was composed of several immature tubules, and these tubules were separated each other by undifferentiated mesenchymal connective tissues. 2. In 3-day and 5-day-old Mongolian gerbils, the arrangement of tubules in the gland was more condensed than that of 1-day-old Mongolian gerbil. The excretory ducts started to appear in the connective tissues located between lobes. 3. In 10-day-old Mongolian gerbil, small lipid vacuoles began to be found in the cytoplasm of the secretory cells of the Harderian gland. There were some mucus-secreting cells within the epithelium of the excretory duct found in the interlobar connective tissues. 4. In 30-day-old Mongolian gerbil, there was markedly increased number of the tubules in the glands. The epithelial cells of the tubules were typically columnar in shape. Most of the columnar epithelial cells contained many small lipid vacuoles, although a few cells contained large lipid vacuoles. 5. In 60-day-old Mongolian gerbil, the Harderian gland exhibited the typical structural characteristics of the adult gland. The mature glandular structures were more distinct than those of 30-day-old animals.