• The Korean Journal of Physiology and Pharmacology
• /
• v.16 no.6
• /
• pp.413-422
• /
• 2012

The purpose of this study is to investigated the mechanism of pharmacological action of local anesthetic and provide the basic information about the development of new effective local anesthetics. Fluorescent probe techniques were used to evaluate the effect of lidocaine HCl on the physical properties (transbilayer asymmetric lateral and rotational mobility, annular lipid fluidity and protein distribution) of synaptosomal plasma membrane vesicles (SPMV) isolated from bovine cerebral cortex, and liposomes of total lipids (SPMVTL) and phospholipids (SPMVPL) extracted from the SPMV. An experimental procedure was used based on selective quenching of 1,3-di(1-pyrenyl)propane (Py-3-Py) and 1,6-diphenyl-1,3,5-hexatriene (DPH) by trinitrophenyl groups, and radiationless energy transfer from the tryptophans of membrane proteins to Py-3-Py. Lidocaine HCl increased the bulk lateral and rotational mobility of neuronal and model membrane lipid bilayes, and had a greater fluidizing effect on the inner monolayer than the outer monolayer. Lidocaine HCl increased annular lipid fluidity in SPMV lipid bilayers. It also caused membrane proteins to cluster. The most important finding of this study is that there is far greater increase in annular lipid fluidity than that in lateral and rotational mobilities by lidocaine HCl. Lidocaine HCl alters the stereo or dynamics of the proteins in the lipid bilayers by combining with lipids, especially with the annular lipids. In conclusion, the present data suggest that lidocaine, in addition to its direct interaction with proteins, concurrently interacts with membrane lipids, fluidizing the membrane, and thus inducing conformational changes of proteins known to be intimately associated with membrane lipid.

• Journal of Microbiology and Biotechnology
• /
• v.20 no.1
• /
• pp.21-29
• /
• 2010

The phenolic compounds are a major contaminant class often found in industrial wastewaters and the biological treatment is an alternative tool commonly employed for their removal. In this sense, monitoring microbial community dynamics is crucial for a successful wastewater treatment. This work aimed to monitor the structure and activity of the bacterial community during the operation of a laboratory-scale continuous submerged membrane bioreactor (SMBR), using PCR and RT-PCR followed by denaturing gradient gel electrophoresis (DGGE) and 16S rRNA libraries. Multivariate analyses carried out using DGGE profiles showed significant changes in the total and metabolically active dominant community members during the 4-week treatment period, explained mainly by phenol and ammonium input. Gene libraries were assembled using 16S rDNA and 16S rRNA PCR products from the fourth week of treatment. Sequencing and phylogenetic analyses of clones from the 16S rDNA library revealed a high diversity of taxa for the total bacterial community, with predominance of Thauera genus (ca. 50%). On the other hand, a lower diversity was found for metabolically active bacteria, which were mostly represented by members of Betaproteobacteria (Thauera and Comamonas), suggesting that these groups have a relevant role in the phenol degradation during the final phase of the SMBR operation.

• Journal of the Korean Electrochemical Society
• /
• v.12 no.1
• /
• pp.61-67
• /
• 2009

Flow-field design has much influence over the performance of proton exchange membrane fuel cell (PEMFC) because it affects the pressure magnitude and distribution of the reactant gases. To obtain the pressure magnitude and distribution of reactant gases in five kinds of flow-field designs, computational fluid dynamics (CFD) analysis was performed. After the CFD analysis, a single cell test was carried out to obtain the performance values. As expected, the pressure differences due to different flow-field configurations were related to the PEMFC performance because the actual performance results showed the same tendency as the results of the CFD analysis. A large pressure drop resulted in high PEMFC performance. The single serpentine configuration gave the highest performance because of the high pressure difference magnitudes of the inlet/outlet. On the other hand, the parallel flow-field configuration gave the lowest performance because the pressure difference between inlet and outlet was the lowest.

• Transactions of the Korean Society of Mechanical Engineers B
• /
• v.33 no.2
• /
• pp.85-92
• /
• 2009

Polymer electrolyte membrane (PEM) fuel cells are a promising technology for short-term power generation required in residential and automobile applications. Proper management of water has been found to be essential for improving the performance and durability of PEM fuel cells. This study investigated the liquid water exhaust capabilities of various flow channels having different geometries and surface properties. Three-pass serpentine flow fields were prepared by patterning channels of 1 mm or 2 mm width onto hydrophilic Acrylic plates or hydrophobic Teflon plates, and the behaviors of liquid water in those flow channels were experimentally visualized. Computational fluid dynamics (CFD) simulations were also conducted to quantitatively assess the liquid water exhaust capabilities of flow channels for PEM fuel cells. Numerical results showed that hydrophobic flow channels have better liquid water exhaust capabilities than hydrophilic flow channels. Flow channels with curved corners showed less droplet stagnation than the channels with sharp corners. It was also found that a smaller width is desirable for hydrophobic flow channels while a larger width is desirable for hydrophilic ones. The above results were explained as being due to the different droplet morphologies in hydrophobic and hydrophilic channels.

• Membrane Journal
• /
• v.26 no.4
• /
• pp.239-252
• /
• 2016

Two-dimensional materials offer unique characteristics for membrane applications to water technology. With its atomic thickness, availability and stackability, graphene in particular is attracting attention in the research and industrial communities. Here, we present a brief overview of the recent research activities in this rising topic with bringing two membrane architecture into focus. Pristine graphene in single- and polycrystallinity poses a unique diffusion barrier property for most of chemical species at broad ambient conditions. If well designed and controlled, physical and chemical perforation can turn this barrier layer to a thinnest feasible membrane that permits ultimate permeation at given pore sizes. For subcontinuum pores, both molecular dynamics simulations and experiments predict potential salt rejection to envisage a seawater desalination application. Another novel membrane architecture is a stack of individual layers of 2D materials. When graphene-based platelets are chemically modified and stacked, the interplanar spacing forms a narrow transport pathway capable of separation of solvated ions from pure water. Bearing unbeknownst permeance and selectivity, both membrane architecture - ultrathin porous graphene and stacked platelets - offer a promising prospect for new extraordinary membranes for water technology applications.

• Transactions of the Korean Society of Mechanical Engineers B
• /
• v.41 no.8
• /
• pp.537-544
• /
• 2017

The purpose of this study is to analyze the flow characteristics when a staggered hollow fiber membrane module is modeled as a porous medium. The pressure-velocity equation was used for modeling the porous medium, using pressure drop data. In terms of flow characteristics, we compared the case of the "porous medium" when the membrane module was modeled as a porous medium with the case of the "membrane module" when considering the original shape of the membrane module. The difference in pressure drop between the "porous medium" and "membrane module" was less than 0.6%. However, the maximum flow velocity and mean turbulent kinetic energy of the "porous medium" were 2.5 and 95 times larger than those of the "membrane module," respectively. Our results indicate that modeling the hollow fiber module as a porous medium is useful for predicting pressure drop, but not sufficient for predicting the maximum flow velocity and mean turbulent kinetic energy.

• Transactions of the Korean hydrogen and new energy society
• /
• v.32 no.4
• /
• pp.245-255
• /
• 2021

Various studies about metallic bipolar plates have been conducted to improve fuel cell performance through flow field design optimization. These research works have been mainly focused on fuel cells for vehicle, but not fuel cells for building. In order to reduce the price and volume of fuel cell stacks for building, it is necessary to apply a metallic flow field, In this study, for a metallic flow field applied to a fuel cell for building, the effect of a change in the flow field shape on the performance of a polymer electrolyte membrane fuel cell was confirmed using a model and experiments with a down-sizing single cell. As a result, the flow field using a metal foam outperforms the channel type flow field because it has higher internal differential pressure and higher reactants velocity in gas diffusion layer, resulting in higher water removal and higher oxygen concentration in the catalyst layer than the channel type flow field. This study is expected to contribute to providing basic data for selecting the optimal flow field for the full stack of polymer electrolyte membrane fuel cells for buildings.

• Korean Chemical Engineering Research
• /
• v.61 no.1
• /
• pp.155-161
• /
• 2023

Ion concentration polarization (ICP) is one of the essential important mechanisms for biomolecule preconcentration devices as well as a fundamental transport phenomenon found in electrodialysis, electrochemical cell, etc. The ICP triggered by externally applied voltage enables the biomolecular analyte to be preconcentrated at an arbitrary position by a locally amplified electric field inside the microchannel. Conventional preconcentration methodologies using the ICP have two limitations: uncertain equilibrium position and hydrodynamic instability of preconcentration plug. In this work, a new preconcentration method in the dead-end microchannel around cation exchange membrane was numerically studied to resolve the limitations. As a result, the numerical model showed that the analyte was concentrated at a shock front developed in a geometrically confined dead-end channel. Furthermore, the electrokinetic behaviors for preconcentration dynamics were analyzed by changing microchannel's applied voltage and volumetric charge concentration of microchannel as key parameters to describe the dynamics. This work would provide an effective means for a point-of-care platform that requires ultra-fast preconcentration method.

• Animal cells and systems
• /
• v.7 no.3
• /
• pp.207-211
• /
• 2003

Caveolin-1 is a principal protein in the plasma membrane microdomains called caveolae. Caveolae play an important role in the transcytosis and pinocytosis. Therefore, caveolin-1 is most likely to work for the membrane dynamic events. In addition, caveolin-1 interacts with various signaling molecules. Although caveolin-1 possesses a variety of physiological functions, its structural properties were little construed. Here we analyzed the structural dynamics of the N-terminal caveolin-1 (residues 1-101), in order to better understand the structural properties in terms of its versatile functionality. We first analyzed its oligomeric form using GST-fused N-terminal domain, revealing that it equilibrates between a dimer and monomers in av concentration-dependent manner. The N-terminal domain of caveolin-1 was previously found to form a heptamer, so that our data suggest the dimeric form as an intermediate structure for the heptamer formation. Then, we obtained the folding profile, which indicated that $\DeltaG_{H2O}\;is\;about\;0.5\;\pm0.03$ kcal/mol. The stability of N-terminal domain is relatively low, indicating that N-terminal domain may not be crystalline. Conclusively, the dynamic and flexible structure of N-terminal domain appears more favorable to maintain the versatile functions of caveolin-1.

• Journal of Embryo Transfer
• /
• v.32 no.3
• /
• pp.105-109
• /
• 2017

Autophagy is an intracellular degradation and recycling system. Oocyte maturation is dynamic process, in which various proteins should be synthesized and degraded. In our previous study, we reported the loci of autophagosome and dynamics of autophagic activity in porcine oocytes during in vitro maturation. In this study, we verified loci of autophagosome in porcine follicular cumulus-oocyte complex by detection of microtubule-associated protein 1A/1B-light chain 3 (LC3) which is the reliable marker of autophagosome. Porcine ovary including various sizes of follicles was fixed within 1 hour after collection from slaughterhouse. After fixation, immunohistochemistry was conducted on sliced ovary tissue containing various sizes of follicles by using LC3 antibody. As a result, LC3 signal was clearly detected in both cumulus and oocytes of various sizes of follicles. We also found ring shaped signal which represent autophagosome near oocyte membrane. Most of the signals in oocytes were localized nearby cellular membrane while evenly dispersed in cumulus cells. Therefore, this result suggests that autophagy occurs in porcine COCs (cumulus-oocyte complexes) at follicular stage.