• Journal of KIBIM
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• v.11 no.4
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• pp.31-41
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• 2021

Digital twins between "BIM Digital Model-Physical Prototype Model" will be built for Noise Barrier tunnel(NBT) that meet the definition of N.G smart city facilities derived from previous studies to build a data flow that connects data at each stage of design, construction, and operation. In this process, BIM design automation tools are created and utilized, and consistent transmission of member and attribute data is performed by converting them into IFC format. Through this, the purpose is to improve the labor-intensive environment required from the design stage of the NBT and to consistently maintain the information required for subsequent production and construction. This includes achieving changes in the construction industry based on digital transformation by unifying various data formats used differently for each industry from design to operation. In addition, it demonstrates that information exchange in the maintenance and management stages is possible based on the data exchange of the established digital twin and aims to improve the existing labor-intensive environment and expand operability between digital and physical information. As suggested in previous studies, the implementation of digital twins in these N.G smart city facilities includes the possibility of building an environment that adds to the possibility of high value-added product platforms as well as the function of big data platforms targeting existing smart cities.

• The Journal of the Korea Contents Association
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• v.22 no.2
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• pp.135-146
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• 2022

This study examines the practice of maeul community media as follows; Who, why, and how the role of participants is related to the operation of maeul community media, and the participation requirements. For this purpose, the maeul newspaper published in rural areas was approached by ethnographic Research, and participation observation, in-depth interview, and content analysis were performed. In conclusion, it was confirmed that harmonious participation of residents is the core condition of maeul community media practice. Residents' participation is based on the requirements of spontaneity, openness, talent donation, and local relationship. Spontaneity is a desire and effort for the activation of the community as a member of the local community. Openness is related to the operation structure of maeul community media where anyone can freely participate. Talent donation is based on their experience, knowledge, and expertise as a concrete practice of resident participation. Local relationship is the network of community that connects spontaneity and practice, and the operating structure of maeul community media. These requirements should be operated positively to ensure the function of maeul community media and sustainability.

• Steel and Composite Structures
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• v.48 no.4
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• pp.367-383
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• 2023

A coupled wall consists of two or more reinforced concrete (RC) shear walls (SWs) connected by RC coupling beams (CBs) or steel CBs (hybrid-coupled walls). To fill the gap in the literature on the plastic hinge length of coupled walls, including coupled and hybrid-coupled shear walls, a parametric study using experimentally validated numerical models was conducted considering the axial stress ratio (ASR) and coupling ratio (CR) as the study variables. A total of sixty numerical models, including both coupled and hybrid-coupled SWs, have been developed by varying the ASR and CR within the ranges of 0.027-0.25 and 0.2-0.5, respectively. A detailed analysis was conducted in order to estimate the ultimate drift, ultimate capacity, curvature profile, yielding height, and plastic hinge length of the models. Compared to hybrid-coupled SWs, coupled SWs possess a relatively higher capacity and curvature. Moreover, increasing the ASR changes the walls' behavior to a column-like member which decreases the walls' ultimate drift, ductility, curvature, and plastic hinge length. Increasing the CR of the coupled SWs increases the walls' capacity and the risk of abrupt shear failure but decreases the walls' ductility, ultimate drift and plastic hinge length. However, CR has a negligible effect on hybrid-coupled walls' ultimate drift and moment, curvature profile, yielding height and plastic hinge length. Lastly, using the obtained results two equations were derived as a function of CR and ASR for calculating the plastic hinge length of coupled and hybrid-coupled SWs.

• Biomedical Science Letters
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• v.28 no.4
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• pp.260-275
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• 2022

Sterile alpha motif (SAM) domains bind to various proteins, lipids, and RNAs. However, these domains have not yet been analyzed as prognostic biomarkers. In this study, SAM domain containing 13 (SAMD13), a member of the SAM domain, was evaluated to identify a novel prognostic biomarker in various human cancers, including hepatocellular carcinoma (HCC). Moreover, we identified a correlation between SAMD13 expression and immune cell infiltration in HCC. We performed bioinformatics analysis using online databases, such as Tumor Immune Estimation Resource, UALCAN, Kaplan-Meier plotter, LinkedOmics, and Gene Expression Profiling Interactive Analysis2. SAMD13 expression in HCC samples was significantly higher than that in normal liver tissue; additionally, SAMD13 was higher in primary tumors, various stages of cancer and grades of tumor, and status of nodal metastasis. Higher SAMD13 expression was also associated with poorer prognosis. SAMD13 expression positively correlated with CD8+ T cells, CD4+ T cells, B cells, neutrophils, macrophages, and dendritic cells. In the analysis of SAMD13 co-expression networks, positively related genes of SAMD13 were associated with a high hazard ratio in different types of cancer, including HCC. In biological function of SAMD13, SAMD13 mainly include spliceosome, ribosome biogenesis in eukaryote, ribosome, etc. These results suggest that SAMD13 may serve as a novel prognostic biomarker for HCC diagnosis and provide novel insights into tumor immunology in HCC.

• Journal of Microbiology and Biotechnology
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• v.33 no.6
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• pp.840-847
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• 2023

Korean ginseng (Panax ginseng C. A. Meyer), a member of the Araliaceae family, is known as a traditional medicinal plant to have a wide range of health properties. Polysaccharides constitute a major component of Korean ginseng, and its berries exhibit immune-modulating properties. The purpose of this study was to investigate the immune effects of crude polysaccharide (GBPC) extracted from Korean ginseng berry on peritoneal macrophages in mice with cyclophosphamide (CY)- induced immunosuppression. BALB/c mice were divided into eight groups: normal control, normal control + CY, levamisole + CY, ginseng + CY, and four concentrations of 50, 100, 250, and 500 mg/kg BW/day of GBPC + CY. Mice were orally administered with samples for 10 days. Immunosuppression was established by treating mice with CY (80 mg/kg BW/day) through intraperitoneal injection on days 4 to 6. The immune function of peritoneal macrophages was then evaluated. Oral administration of 500 mg/kg BW/day GBPC resulted in proliferation, NO production, and phagocytosis at 100%, 88%, and 91%, respectively, close to the levels of the normal group (100%) of peritoneal macrophages. In CY-treated mice, GBPC of 50-500 mg/kg BW/day also dose-dependently stimulated the proliferation, NO production, and phagocytosis at 56-100%, 47-88%, and 53-91%, respectively, with expression levels of immune-associated genes, such as iNOS, COX-2, IL-1β, IL-6, and TNF-α, of about 0.32 to 2.87-fold, compared to those in the CY group. GBPC could be a potential immunomodulatory material to control peritoneal macrophages under an immunosuppressive condition.

• The KIPS Transactions:PartC
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• v.16C no.6
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• pp.791-800
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• 2009

Multicasting is fundamental to many ad hoc network applications requiring collaboration of multiple nodes in a group. A general approach is to construct an overlay tree and to deliver a multicast packet to multiple receivers over the tree. This paper proposes adaptive overlay trees (AOTs) on wireless ad hoc networks of static nodes for delay- and energy-efficient multicast. A tradeoff function is derived, and an algorithm for AOT construction is developed. Note here that the requirements of delay and energy consumption may vary with different classes of applications. By adjusting parameters in the tradeoff function, different AOTs can be adaptively chosen for different classes of applications. An AOT is constructed in O(ke) time where e is the number of wireless links in a network and k is the number of member nodes in a multicast group. The simulation study shows that AOT adaptively provides tradeoffs between the fastest multicast (which is the choice if delay is the most important factor) and the most energy efficient multicast (which is used when energy consumption is the primary concern). In other words, one of AOTs can be appropriately chosen in accordance with the operation requirement.

• Proceedings of the KSAR Conference
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• 2001.03a
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• pp.54-54
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• 2001

Human chorionic gonadotropin (hCG) is a member of the glycoprotein hormone family which includes FSH, hCG, TSH. These hormone family is characterized by a heterodimeric structure composed a common $\alpha$-subunit noncovalently linked to a hormone specific $\beta$-subunit. The correct conformation of the heterodimer is also important for efficient secretion, hormone-specific post-translational modifications, receptor binding and signal transduciton. To determine $\alpha$ and $\beta$-subunits can be synthesized as a single polypeptide chain (tethered-hCG) and also display biological activity, the tethered-hCG molecule by fusing the carboxyl terminus of the hCG $\beta$-subunit to the amino terminus of the $\alpha$-subunit was constructed and transfected into chinese hamster ovary (CHO-K1) cells. We also constructed C-terminal deletion mutants (D9l, D89, D88, D87, D86, D84, D83) of single chain hCG to determine the biological function (secretion, LH-activity, receptor binding, cAMP production) of these mutants. Between six and eight stably transfected pools of cells expressing wild type and mutant hCGs were selected for neomycin resistant. The hCGs secreted by the stably transfected cells into serum-free media were collected and quantified by radioimmunoassay, as described in protocol (DPC(hCG IRMA). LH activity was in terms of testosterone production and aromatase activity in primary cultured rat Leydig cells. The tethered-wthCG was efficiently secreted and showed similar LH-like activity to the dimeric hCG. The D83hCG mutant was not detected in this assay. It is suggest that hCG C-terminal part is very important for hCG secretion. Now, we checking the LH-like activity of these mutant hCGs. These data indicate that the constructs of tethered molecule will be useful in the study of mutants that affect subunit association and/or secretion.

• Journal of Korean Society of Forest Science
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• v.107 no.1
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• pp.59-70
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• 2018

The objective of this study was to develop erosion control dam for preventing disaster in consideration of characteristics of forested catchment near urban area, and to assess its stability and functionality to see its practicability in the field. Two types of hybrid erosion control dams were developed including debris flow prevention dam by using pillar and float board screen type and debris flow control dam by using groyne. Also, review about their static (sliding, overturning, bearing capacity) and dynamic (member force) stability was carried out. According to the result, most of the assessed items met standard safety level although there were some cases where assessed items were short of stability criteria against impact. Also, after miniature flume experiments based on the developed erosion control dam to prove structure function (material catch, deposit), it turned out the dam decreased flow sediment amount and velocity while increasing sediment-capturing capacity by 3.5 times on average compared to the one controlled without erosion control dam. When function of erosion control dam for forested catchment near urban area is quantified based on future flume experiments in a variety of conditions, the dams can be practically used in the urban area, contribution to effectively reducing debris flow damage.

• Clinical and Experimental Reproductive Medicine
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• v.47 no.3
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• pp.194-206
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• 2020

Objective: The aim of this study was to investigate microRNAs (miRNAs) related to follicle-stimulating hormone (FSH) responsiveness using miRNA microarrays and to identify their target genes to determine the molecular regulatory pathways involved in FSH signaling in KGN cells. Methods: To change the cellular responsiveness to FSH, KGN cells were treated with FSH receptor (FSHR)-specific small interfering RNA (siRNA) followed by FSH. miRNA expression profiles were determined through miRNA microarray analysis. Potential target genes of selected miRNAs were predicted using bioinformatics tools, and their regulatory function was confirmed in KGN cells. Results: We found that six miRNAs (miR-1261, miR-130a-3p, miR-329-3p, miR-185-5p, miR-144-5p and miR-4463) were differentially expressed after FSHR siRNA treatment in KGN cells. Through a bioinformatics analysis, we showed that these miRNAs were predicted to regulate a large number of genes, which we narrowed down to cytochrome P450 family 19 subfamily A member 1 (CYP19A1) and estrogen receptor alpha (ESR1) as the main targets for miR-4463. Functional analysis revealed that miR-4463 is a regulatory factor for aromatase expression and function in KGN cells. Conclusion: In this study, we identified differentially expressed miRNAs related to FSH responsiveness. In particular, upregulation of miR-4463 expression by FSHR deficiency in human granulosa cells impaired 17β-estradiol synthesis by targeting CYP19A1 and ESR1. Therefore, our data might provide novel candidates for molecular biomarkers for use in research into poor responders.

• Journal of Life Science
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• v.17 no.2 s.82
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• pp.235-240
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• 2007

AvrRpt2 protein triggers hypersensitive response (HR) and strong disease resistance when it is translocated from a bacterial pathogen Pseudomonas sp. to host plant cells containing a cognate RPS2 resistance protein through Type III Secretion System (TTSS). However, AvrRpt2 protein can function as the effector that suppresses a basal defense and enhances the disease symptom when functional RPS2 resistance protein is absent in the infected plant cells. Using Affymetrix Arabidopsis DNA chip, we found that many genes were specifically regulated by AvrRpt2 protein in the rps2 Arabidopsis mutant. Here, we showed that expression of AtERF11 that is known as a member of B1a subcluster of AP2/ERF transcription factor family was down regulated specifically by AvrRpt2. To determine its function in plant resistance, we also generated the Arabidopsis thaliana transgenic plants constitutively overexpressing AtERF11 under CaMV 355 promoter, which conferred an enhanced resistance against a bacterial pathogen, Pseudomonas syringae pv. tomato DC3000. Thus, these results collectively suggest that AtERF11 plays a role as a positive regulator for disease resistance against biotrophic bacterial pathogen in plant.