• 한국자원식물학회지
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• 제22권4호
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• pp.312-316
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• 2009

In the present study, the anti-cancer activity of 80% ethanol extracts from 120 kinds of medicinal herbs and native plants were investigated. Among them, the barks of Melia azedarach L. var. japonica Makino showed the highest cytotoxicity in HCT-15 human colon cancer cell. With this result, we carried out hollow fiber (HF) assay and anti-metastasis study to confirm the anti-cancer effects of M. azedarach var. japonica. In MTT assay, M. azedarach var. japonica.inhibited the proliferation of HCT-15 cells in dose-dependent manner. HF assay was carried out using A549 human adenocarcinoma cell, HCT-15 and SK-Hep1 human liver cancer cell via intraperitoneal (IP) and subcutaneous (SC) site. As a results, SK-Hep1 implanted in IP site showed the highest cytotoxicity. The result from metastatic model using B16/BL6 mouse corresponded to that of HF assay. These results suggest that the ethanol extract from M. azedarach var. japonica. might have a potent anti-cancer activity and advanced study is needed for the development of novel natural anti-cancer drug.

• Toxicological Research
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• 제28권1호
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• pp.57-65
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• 2012

In this study, the 4-week oral toxicity and anti-cancer activity of the hexane layer of Melia azedarach L. var. japonica Makino's bark extract were investigated. We carried out a hollow fiber (HF) assay and 28-day repeated toxicity study to confirm the anti-cancer effect and safety of the hexane layer. The HF assay was carried out using an A549 human adenocarcinoma cell via intraperitoneal (IP) site with or without cisplatin. In the result, the 200 mg/kg b.w of hexane layer with 4 mg/kg b.w of cisplatin treated group, showed the highest cytotoxicity aginst A549 carcinoma cells. For the 28-day repeated toxicity study, 6 groups of 10 male and female mice were given by gavage 200, 100, or 50 mg/kg b.w hexane layer with or without 4 mg/kg b.w of cisplatin against body weight, and were then sacrificed for blood and tissue sampling. The subacute oral toxicity study in mice with doses of 200, 100, and 50 mg/kg b.w hexane layer showed no significant changes in body weight gain and general behavior. The cisplatin-treated group significantly decreased in body weight compared to the control group but regained weight with 100 and 200 mg/kg b.w of hexane layer. The biochemical analysis showed significant increase in several parameters (ALT, total billirubin, AST, creatinine, and BUN) in cisplatin-treated groups. However, in the group given a co-treatment of hexane layer (200 mg/kg b.w), levels of these parameters decreased. In hematological analysis, cisplatin induced the reduction of WBCs and neutrophils but co-treatment with hexane layer (100 and 200 mg/kg b.w) improved these toxicities caused by cisplatin. The histological profile of the livers showed eosinophilic cell foci in central vein and portal triad in cisplatin treated mice. These results show that hexane layer might have an anti-cancer activity and could improve the toxicity of cisplatin.

• 동의생리병리학회지
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• 제25권2호
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• pp.270-274
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• 2011

The prevalence of atopic dermatitis has markedly increased in recent years but the mechanism has not been clearly revealed. Recent study exhibited that atopic dermatitis was exacerbated by the increase of proteinase-activated receptor (PAR)-2 expression, which activated $I{\kappa}B$ kinase --> nuclear factor kappa B. Therefore, we determined whether the allergens of dust mites induced the expression of PAR-2, intercellular adhesion molecule-1 (ICAM-1, adehision molecule), interleukins (IL)-6 in HaCaT keratocytes and which herbal 1,3-butylene glycol extracts (Mori Cortex Radicis, Sanguisorba officinalis L., Arctium lappa Linne, Torilis japonica DC, Melia azedarach Linne var. japoinca Makino) suppressed their expression. Dust mite allergen increased PAR-2, ICAM-1 and IL-6 expression in HaCaT cells in a dose-dependent manner up to $3{\mu}g/mL$ but their expression reached the plateau over the dosages. The allergen ($3{\mu}g/mL$) also secreted more cytokines such as tumor necrosis factor (TNF)-${\alpha}$ and IL-6 into the media. Among five different herbal extracts ($50{\mu}g/mL$), Mori Cortex Radicis and Sanguisorba officinalis L. suppressed the PAR-2, ICAM-1 and IL-6 expression in HaCaT cells, which was activated by dust mite allergen ($3{\mu}g/mL$) and they also reduced the secretion of TNF-${\alpha}$ and IL-6 into the media. In conclusion, Mori Cortex Radicis and Sanguisorba officinalis L. can effectively reduce the prevalence and progression of atopic dermatitis by dute mite allergen.

• 한국환경생태학회지
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• 제24권2호
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• pp.209-221
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• 2010

본 연구는 해안 염해지 수목 식재를 위한 내염성 및 내조성 수종을 선발하고자 한국 서해안 해안림의 식생조사와 토양염분도를 조사 분석하였다. 조사지의 토양염분은 전체 평균 $EC_{1:5}$ 0.11dS$m^{-1}$이었고, 최저 0.00dS$m^{-1}$, 최고 0.68dS$m^{-1}$이었다. 토양염분($EC_{1:5}$)은 해안 정선으로 부터 내륙 방향으로 갈수록 낮았으며, Ⅰ지대>Ⅱ지대>Ⅲ지대>Ⅳ 지대 순으로 각각 $EC_{1:5}$ 0.14dS$m^{-1}$, 0.11dS$m^{-1}$, 0.10dS$m^{-1}$, 0.08dS$m^{-1}$이었다. 출현한 자생식물은 52과 104속 24변종 157종 총 181분류군이었다. 토양염분이 가장 높은 단계인 $EC_{1:5}$ 0.51dS$m^{-1}$를 초과하는 곳에서 EC의 특이값 또는 이상치의 수준으로 여러 번 출현하는 수종은 곰솔, 청미래덩굴, 떡갈나무, 졸참나무 등이었다. $EC_{1:5}$ 0.41~0.50dS$m^{-1}$에서는 $EC_{1:5}$ 0.40dS$m^{-1}$ 이하에 속하는 식물들 이외에도 왕자귀나무, 멀구슬나무, 계요등으로 출현빈도가 매우 낮았다. 전체 지대에 출현하는 수종은 리기다소나무, 소나무, 곰솔, 노간주나무 등 이었고, 중요도가 높은 수종은 소나무, 곰솔, 이대, 청미래덩굴, 굴피나무 등이었다. 이러한 수종은 조사지의 자생 수종들 중에서 다른 수종에 비하여 상대적으로 내조성이 강한 것으로 판단되었다.