• Journal of Applied Biological Chemistry
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• 제65권3호
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• pp.231-237
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• 2022

Biorenovation은 미생물의 효소 작용을 통해 기존의 화합물을 새로운 화합물로 전환시키는 방법이다. 본 연구에서는 biorenovation을 Luteolin에 적용하여 Luteolin-7-O-glucoside (L7G)를 합성하였으며 L7G의 미백 기능성을 평가하고자 α-MSH로 유도된 B16F10 mouse melanoma 세포에서 실험을 진행하였다. L7G는 Luteolin의 높은 세포독성을 개선하였으며 세포 독성이 나타나지 않는 농도에서 melanin 합성 및 tyrosinase 생성을 유의하게 억제하였다. 또한 western blot을 통해 멜라닌의 합성 인자들의 발현을 조사한 결과 tyrosinase와 MITF의 발현이 효과적으로 억제되는 것을 확인하였다. 결론적으로 우리는 L7G가 멜라닌의 합성을 억제함으로써 피부 미백에 긍정적인 영향을 나타낼 수 있음을 확인하였으며 이러한 결과를 근거로 L7G가 미백 기능성 원료로써 활용 가능함을 제안한다.

• 대한의용생체공학회:의공학회지
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• 제36권1호
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• pp.22-30
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• 2015

Melanoma is originated from the melanocyte producing the melanin which determines the complexion, and it has the highest mortality among skin cancers. Acral lentiginous melanoma(ALM) arises from extremities such as hands, feet or fingernails. Since the appearance of ALM is different from melanoma on the body, conventional auto diagnosis systems for melanoma is inappropriate to detect ALM. Therefore, ALM is typically difficult to distinguish from general nevus, resulting in delayed diagnosis and bad prognosis. In this paper, we firstly introduce a determination method for ALM by dermatologists and propose a method to rotate dermoscopic images automatically as a pre-processing for facilitating the easy determination of ALM and to select the optimal value of the Gaussian differentiation filter parameter which is significant for precise pattern extraction using the scale space analysis. From experimental results, it is shown that there exists the consistency between empirical values of the Gaussian differential filter parameter and optimal values derived from the scale space analysis to distinguish nevus and ALM.

• 생약학회지
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• 제42권4호
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• pp.323-328
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• 2011

We investigated antioxidative activity, tyrosinase inhibitory activity, and melanin production inhibitory activity of the methanol extract of Paeoniae Radix and its fractions. The total polyphenol content of the extract was 73.45 mg/g, and content of the ethyl acetate fraction was 514.50 mg/g as the highest content of fractions. In DPPH radical scavenging ability, $SC_{50}$ values of the ethyl acetate was 3.86 ${\mu}g/ml$ as a result of greater activity in the positive control (ascorbic acid). Moreover, tyrosinase inhibitory activity of the ethyl acetate fraction showed higher activity than arbutin used as a positive control. In the cytotoxicity measurement by MTT assay, the extract and fractions were exhibited cell viabilities of 76.96~157.26% against Raw 264.7 and B16F10 cell in concentration of 10~100 ${\mu}g/ml$. In nontoxic concentration range, the ethyl acetate fraction showed strong melanin production inhibitory effect in ${\alpha}$-melanocyte stimulating hormone (${\alpha}$-MSH)-stimulated B16F10 cell. As a result, the ethyl acetate fraction of the methanol extract from Paeoniae Radix could be applicable to functional materials for skin-whitening agents.

• 한국식품과학회지
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• 제43권2호
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• pp.240-242
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• 2011

액체배양한 송이버섯 균사체와 배양액의 tyrosianse 억제활성과 melanocyte에서의 세포독성 및 멜라닌 생성억제효과를 검정한 결과 배양액 농축물은 큰 효과가 없었으나 송이버섯 균사체 추출물은 100 ppm에서 38.6%의 tyrosianse 억제활성을 나타내었을 뿐만 아니라 melan-a 세포에서 세포독성 없이 세포생존율 대비 19%의 멜라닌 생성량을 감소시켰다. 따라서 배양된 송이버섯균사체는 피부색소조절을 위한 소재로써 사용되어질 수 있는 큰 가능성을 가지고 있다고 판단된다.

• 약학회지
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• 제48권6호
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• pp.374-378
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• 2004

To investigate the whitening activity of Laminaria japonicus, we measured the effect of hot-water extracts on melanin production in B16 melanoma cells. Melanocyte stimulating h ormone (MSH, $1{\mu}M$)-stimulated melanin production in B16 melanoma cells was significantly inhibited by fucoidan and hot-water extracts, but not by alginate. The purified tyrosinase activity was not affected by hot-water extracts, alginate and fucoidan. However, tyrosinase expression was significantly inhibited by fucoidan and hot-water extract in Western blot. These results suggest that inhibitory mechanism of hot-water extracts on melanin production may be due to the inhibition of tyrosinase expression but not to direct inhibition of tyrosinase, such an effect of which may be dependent on fucoidan.

• 대한화장품학회지
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• 제28권3호
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• pp.63-90
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• 2002

멜라닌의 정량은 인체에서 화장품의 미백효과를 평가하기 위한 가장 좋은 방법이지만 인체의 멜라닌 측정을 위한 높은 정확성을 가진 non-invasive방법은 아직 확립되지 않은 실정이다. 피부에서 화장품의 미백효과는 피부색의 밝기로 나타낼 수 있으므로 화장품의 미백효력 평가를 위한 재현성있는 방법으로는 피부색측정을 하는 것이 합리적이다 이에 colorimeter, mexameter와 전문가의 육안평가같은 여러 기기나 분석법이 사용되었다. 이 강연에서는 미백효과에 대한 평가를 위한 다양한 평가방법에 대해 자세히 보고하고 각 방법에 대하여 토의하게 될 것이다 그리고 간단히 임상시험에 대한 결과를 보고하고 마지막으로 melanocyte를 정량하는 새로운 non-invasive방법에 대한 model을 제시하려고 한다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.95-95
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• 2018

In this study, we investigated the effect of branch extracts from Vaccinium oldhamii (VOB) on melanin synthesis in B16F10 cells. VOB promoted melanin production in absence or presence of ${\alpha}$-melanocyte-stimulating hormone (${\alpha}-MSH$) in B16F10 cells. However, VOB did not affect the expression of tyrosinase and TRP-1 associated with melanin synthesis at the mRNA and protein level in B16F10. But, VOB decreased TRP-2 protein level and induced tyrosinase activation in B16F10 cells. Inhibition of tyrosinase activity and tyrosinase knockdown attenuated VOB-mediated melanin synthesis. In conclusion, it is thought that VOB may stimulate melanin synthesis through activating tyrosinase activity.

• BMB Reports
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• 제43권12호
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• pp.824-829
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• 2010

Melanin synthesis is regulated by melanocyte specific enzymes and related transcription factors. $\beta$-carboline alkaloids including harmaline and harmalol are widely distributed in the environment including several plant families and alcoholic beverages. Presently, melanin content and tyrosinase activity were increased in melanoma cells by harmaline and harmalol in concentration- and time-dependent manners. Increased protein levels of tyrosinase, tyrosinase-related protein-1 (TRP-1), and TRP-2 were also evident. In addition, immunofluorescence and Western blot analyses revealed harmaline and harmalol increased cAMP response element binding protein phosphorylation and microphthalmia-associated transcription factor expression. In addition to studying the signaling that leads to melanogenesis, roles of the p38 MAPK pathways by the harmaline and harmalol were investigated. Harmaline and harmalol induced time-dependent phosphorylation of p38 MAPK. Harmaline and harmalol stimulated melanin synthesis and tyrosinase activity, as well as expression of tyrosinase and TRP-1 and TRP-2 indicating that these harmaline and harmalol induce melanogenesis through p38 MAPK signaling.

• Biomolecules & Therapeutics
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• 제22권1호
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• pp.35-40
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• 2014

Resveratrol is a polyphenolic compound found in various natural products such as grapes and berries and possesses anti-cancer, anti-hyperlipidemia, and anti-aging properties. Recently, it has been reported that resveratrol inhibits ${\alpha}$-melanocyte-stimulating hormone signaling, viability, and migration in melanoma cells. However, these effects have not been confirmed in vivo, specifically brownish guinea pigs. To evaluate the potential of resveratrol as a regulator of melanin for hyperpigmentation therapy, the influence of resveratrol on pigmentation was investigated by ultraviolet B-induced hyperpigmentation in brownish guinea pig skin. We found that resveratrol reduced the expression of melanogenesis-related proteins tyrosinase, tyrosinase-related proteins 1 and 2, and microphthalmia-associated transcription factor in melanoma cells. Furthermore, topical application of resveratrol was demonstrated to significantly decrease hyperpigmentation on ultraviolet B-stimulated guinea pig skin in vivo. Based on our histological data, resveratrol inhibits melanin synthesis via a reduction in tyrosinase-related protein 2 among the melanogenic enzymes. This study is the first to provide evidence supporting resveratrol as a depigmentation agent, along with further clinical investigation of resveratrol in ultraviolet B-induced skin disorders such as hyperpigmentation and skin photoaging.

• 생약학회지
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• 제51권3호
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• pp.171-177
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• 2020

The purpose of this study was to investigate the anti-melanogenic effects of the extracts from Decaschistia intermedia craib (EDI). In this study, we examined the effects of EDI on mushroom tyrosinase activity in in vitro, melanin contents, and expression levels of mRNA and proteins of melanogenesis-related genes in B16F10 melanoma cells. The treatment of EDI significantly decreased both tyrosinase activity and melanin contents in B16F10 cells with dose-dependent manner. In addition, we found that the expression of mRNA or proteins of melanogenic proteins, such as, a-melanocyte-stimulating hormone (a-MSH)-induced microphthalmia associated transcription factor (MITF), tyrosinase, tyrosinase related protein-1 (TRP-1), and TRP-2 was significantly downregulated with dose-dependent manner in the EDI-treated B16F10 cells compared to controls. Our results suggest that the EDI inhibits cellular melanogenesis through downregulation of a-MSH-stimulated melanin synthesis. Thus EDI may potentially be an effective whitening agent.