• Title/Summary/Keyword: Mel-B

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Synthesis of Borosilicate Zeotypes by Steam-assisted Conversion Method (수증기 쪼임법에 의한 제올라이트형 보로실리케이트 제조방법)

  • Mansour, R.;Lafjah, M.;Djafri, F.;Bengueddach, A.
    • Journal of the Korean Chemical Society
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    • v.51 no.2
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    • pp.178-185
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    • 2007
  • Intermediate pentasil borosilicate zeolite-like materials have been crystallized by a novel method named steam-assisted conversion, which involves vapor-phase transport of water. Indeed, amorphous powders obtained by drying Na2O.SiO2.B2O3.TBA2O gels of various compositions using different boron sources are transformed into crystalline borosilicate zeolite belonging to pentasil family structure by contact with vapors of water under hydrothermal conditions. Using a variant of this method, a new material which has an intermediate structure of MFI/MEL in the ratio 90:10 was crystallized. The results show that steam and sufficiently high pH in the reacting hydrous solid are necessary for the crystallization to proceed. Characterization of the products shows some specific structural aspects which may have its unique catalytic properties. X-ray diffraction patterns of these microporous crystalline borosilicates are subjected to investigation, then, it is shown that the product structure has good crystallinity and is interpreted in terms of regular stacking of pentasil layers correlated by inversion centers (MFI structure) but interrupted by faults consisting of mirror-related layers (MEL structure). The products are also characterized by nitrogen adsorption at 77 K that shows higher microporous volume (0.160 cc/g) than that of pure MFI phase (0.119 cc/g). The obtained materials revealed high surface area (~600 m2/g). The infrared spectrum reveals the presence of an absorption band at 900.75 cm-1 indicating the incorporation of boron in tetrahedral sites in the silicate matrix of the crystalline phase.

Cytotoxic Activity of Leguminous Seed Extracts against Human Tumor Cell Lines

  • Lee, Hoi-Seon;Lee, Jeong-Ock;Lee, Hee-Kwon;Oh, Jong-Hwan;Ahn, Young-Joon
    • Applied Biological Chemistry
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    • v.41 no.4
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    • pp.246-250
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    • 1998
  • The cytotoxic activity of methanol extracts of 25 leguminous seeds in vitro was evaluated by sulforhodamine B assay, using the five human solid A549 lung, SK-OV-2 ovarian, SK-MEL-2 melanoma, XF-498 CNS and HCT-15 colon tumor cell lines. The responses varied with both cell line arid leguminous seed used. Extracts of Canavalia lineata and Glycine soja revealed potent cytotoxic activity against A549 arid SK-MEL-2 cell lines. Moderate activity was observed in the extracts of Cassia obtusifolia and Glyeine max var. chungtae, and C. lineata and Vigna angulasis against SK-MEL-2 and HCT-15 cell lines, respectively. The other seed extracts were ineffective against model tumor cell lines. Because of their potent cytotoxic activities, the activity of each solvent fraction from C. lineata and G. soja was determined and the potent activity was produced from their chloroform fractions. As a naturally occurring therapeutic agent, leguminous seeds described could be useful for developing new types of anti-tumor agents.

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Design of Speech Enhancement U-Net for Embedded Computing (임베디드 연산을 위한 잡음에서 음성추출 U-Net 설계)

  • Kim, Hyun-Don
    • IEMEK Journal of Embedded Systems and Applications
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    • v.15 no.5
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    • pp.227-234
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    • 2020
  • In this paper, we propose wav-U-Net to improve speech enhancement in heavy noisy environments, and it has implemented three principal techniques. First, as input data, we use 128 modified Mel-scale filter banks which can reduce computational burden instead of 512 frequency bins. Mel-scale aims to mimic the non-linear human ear perception of sound by being more discriminative at lower frequencies and less discriminative at higher frequencies. Therefore, Mel-scale is the suitable feature considering both performance and computing power because our proposed network focuses on speech signals. Second, we add a simple ResNet as pre-processing that helps our proposed network make estimated speech signals clear and suppress high-frequency noises. Finally, the proposed U-Net model shows significant performance regardless of the kinds of noise. Especially, despite using a single channel, we confirmed that it can well deal with non-stationary noises whose frequency properties are dynamically changed, and it is possible to estimate speech signals from noisy speech signals even in extremely noisy environments where noises are much lauder than speech (less than SNR 0dB). The performance on our proposed wav-U-Net was improved by about 200% on SDR and 460% on NSDR compared to the conventional Jansson's wav-U-Net. Also, it was confirmed that the processing time of out wav-U-Net with 128 modified Mel-scale filter banks was about 2.7 times faster than the common wav-U-Net with 512 frequency bins as input values.

Effects of Smilax China L. on the Growth of Skin Cancer Cells (토복령(土茯笭)이 피부암 세포의 성장에 미치는 영향)

  • Si-Yeol Song;Min-Yeong Jung;Jeong-Hwa Choi;Soo-Yeon Park
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.37 no.1
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    • pp.1-16
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    • 2024
  • Objectives : We aimed to study the effect of Smilax China L.(SCL), which has anti-inflammatory, antioxidant, and anticancer effects, on the growth of skin cancer cells. Methods : HaCaT cells, a normal human cell line, and skin cancer cells including A431, SK-MEL-5 and SK-MEL-28 cells were treated with Smilax China L. ethanol extract(SCL-EtOH) at concentrations of 5, 10, 20 and 40㎍/㎖. Meanwhile, JB6 Cl41, a normal mouse epithelial cell line, was treated with epidermal growth factor(EGF) and phorbol 12-myristate 13-acetate(TPA), an inflammatory factor, to induce cell transformation and treated with SCL-EtOH. In addition, we treated SK-MEL-5 and SK-MEL-28 cells with SCL-EtOH at various concentrations and checked the effect on the cell cycle. Results : As a result, it showed no toxicity to HaCaT cells up to the highest concentration of 40㎍/㎖, and significant cell growth inhibition to A431, SK-MEL-5 and SK-MEL-28 cells in a time- and concentration-dependent manner. In addition, as a result of checking the shape of skin cancer cells according to SCL-EtOH treatment, it was observed that as the concentration increased, the number of normally attached and growing cells decreased and the shape of the cells changed. Colony formation was significantly reduced in a concentration-dependent manner in JB6 Cl41 cells treated with EGF or TPA. Flow cytometry analysis with propidium iodide(PI) staining showed that SCL-EtOH induced the G2/M phase arrest. We further confirmed the decrease in Cyclin B1 expression and increase in p27 expression associated with the G2/M phase of the cell cycle through western blot analysis. Flow cytometry analysis confirmed that SCL-EtOH induced cell apoptosis. Furthermore, through Western blot analysis, it was observed that the expression of cleaved-caspase-7, which is related to apoptosis, increased. Finally, it was confirmed that the expression of COX-2, an inflammatory marker protein, decreased in a concentration-dependent manner with SCL-EtOH. Conclusions : Through the above results, we have established a basis for applying SCL to the treatment of skin cancer.

A Study on the Human Auditory Scaling (인간의 청각 척도에 관한 고찰)

  • Yang, Byung-Gon
    • Speech Sciences
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    • v.2
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    • pp.125-134
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    • 1997
  • Human beings can perceive various aspects of sound including loudness, pitch, length, and timber. Recently many studies were conducted to clarify complex auditory scales of the human ear. This study critically reviews some of these scales (decibel, sone, phon for loudness perception; mel and bark for pitch) and proposes to apply the scales to normalize acoustic correlates of human speech. One of the most important aspects of human auditory perception is the nonlinearity which should be incorporated into the linear speech analysis and synthesis system. Further studies using more sophisticated equipment are desirable to refine these scales, through the analysis of human auditory perception of complex tones or speech. This will lead scientists to develop better speech recognition and synthesis devices.

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Two-step a priori SNR Estimation in the Log-mel Domain Considering Phase Information (위상 정보를 고려한 로그멜 영역에서의 2단계 선험 SNR 추정)

  • Lee, Yun-Kyung;Kwon, Oh-Wook
    • Phonetics and Speech Sciences
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    • v.3 no.1
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    • pp.87-94
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    • 2011
  • The decision directed (DD) approach is widely used to determine a priori SNR from noisy speech signals. In conventional speech enhancement systems with a DD approach, a priori SNR is estimated by using only the magnitude components and consequently follows a posteriori SNR with one frame delay. We propose a phase-dependent two-step a priori SNR estimator based on the minimum mean square error (MMSE) in the log-mel spectral domain so that we can consider both magnitude and phase information, and it can overcome the performance degradation caused by one frame delay. From the experimental results, the proposed estimator is shown to improve the output SNR of enhanced speech signals by 2.3 dB compared to the conventional DD approach-based system.

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Cytotoxicity of steroid-saponins from the tuber of Liriope platyphylla W. T. (맥문동(Liriope platyphylla W. T.) 스테로이드 사포닌의 항암활성)

  • Cho, Sung-Ji;Bang, Myun-Ho;Lee, In-Ja;Park, Chang-Gi;Kim, Moo-Sung;Kim, Keum-Sook;Sung, Jae-Duk;Baek, Nam-In
    • Applied Biological Chemistry
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    • v.41 no.5
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    • pp.390-394
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    • 1998
  • The tuber of Liriope platyphylla was extracted with 80% aqueous MeOH and solvent-fractionated with EtOAc, n-BuOH and $H_2O$. Evaluation of growth inhibitory activity of each fractions on various carcinoma cells, A549, SK-OV-3, SK-Mel-2, XF-498 and HCT-15, indicated the n-BuOH fraction to be the highest in the activity. From the fraction, two saponin compounds were isolated through Amberlite XAD-II and silica gel column chromatographies, repeatedly, and their chemical structures were elucidated as spicatoside A and B by interpretation of spectral data, NMR and IR, and adaptation of acid hydrolysis. Spicatoside A showed growth inhibitory activity on carcinoma cells, and the $IC_{50}$ values against A549, SK-OV-3, SK-Mel-2, XF-498 and HCT-15 cells were determined to be 17.3, 21.7, 14.9, 18.8 and $15.6\;{\mu}g/ml$, respectively.

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Feasibility of Deep Learning-Based Analysis of Auscultation for Screening Significant Stenosis of Native Arteriovenous Fistula for Hemodialysis Requiring Angioplasty

  • Jae Hyon Park;Insun Park;Kichang Han;Jongjin Yoon;Yongsik Sim;Soo Jin Kim;Jong Yun Won;Shina Lee;Joon Ho Kwon;Sungmo Moon;Gyoung Min Kim;Man-deuk Kim
    • Korean Journal of Radiology
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    • v.23 no.10
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    • pp.949-958
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    • 2022
  • Objective: To investigate the feasibility of using a deep learning-based analysis of auscultation data to predict significant stenosis of arteriovenous fistulas (AVF) in patients undergoing hemodialysis requiring percutaneous transluminal angioplasty (PTA). Materials and Methods: Forty patients (24 male and 16 female; median age, 62.5 years) with dysfunctional native AVF were prospectively recruited. Digital sounds from the AVF shunt were recorded using a wireless electronic stethoscope before (pre-PTA) and after PTA (post-PTA), and the audio files were subsequently converted to mel spectrograms, which were used to construct various deep convolutional neural network (DCNN) models (DenseNet201, EfficientNetB5, and ResNet50). The performance of these models for diagnosing ≥ 50% AVF stenosis was assessed and compared. The ground truth for the presence of ≥ 50% AVF stenosis was obtained using digital subtraction angiography. Gradient-weighted class activation mapping (Grad-CAM) was used to produce visual explanations for DCNN model decisions. Results: Eighty audio files were obtained from the 40 recruited patients and pooled for the study. Mel spectrograms of "pre-PTA" shunt sounds showed patterns corresponding to abnormal high-pitched bruits with systolic accentuation observed in patients with stenotic AVF. The ResNet50 and EfficientNetB5 models yielded an area under the receiver operating characteristic curve of 0.99 and 0.98, respectively, at optimized epochs for predicting ≥ 50% AVF stenosis. However, Grad-CAM heatmaps revealed that only ResNet50 highlighted areas relevant to AVF stenosis in the mel spectrogram. Conclusion: Mel spectrogram-based DCNN models, particularly ResNet50, successfully predicted the presence of significant AVF stenosis requiring PTA in this feasibility study and may potentially be used in AVF surveillance.

Apoptosis and inhibition of human epithelial cancer cells by ZnO nanoparticles synthesized using plant extract

  • Koutu, Vaibhav;Rajawat, Shweta;Shastri, Lokesh;Malik, M.M.
    • Advances in nano research
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    • v.7 no.4
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    • pp.233-240
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    • 2019
  • The present research work reports in-vitro anti-cancer activity of biologically synthesized ZnO nanoparticles (ZnO NPs) against human carcinoma cells viz SCC-40, SK-MEL-2 and SCC-29B using Sulforhodamine-B (SRB) Assay. ZnO NPs were synthesized by a unique and novel biological route using Temperature-gradient phenomenon where the extract of combination of Catharanthus roseus (L.) G. Don (C. roseus), Azadirachta indica (A. indica), Ficus religiosa (F. religiosa) and NaOH solution were used as synthesis medium. The morphology of the ZnO NPs was characterized by Transmission Electron Microscopy (TEM). TEM images reveal that particle size of the samples reduces from 76 nm to 53 nm with the increase in reaction temperature and 68 nm to 38 nm with the increase in molar concentration of NaOH respectively. XRD study confirms the presence of elements and reduction in crystallite size with increase in reaction temperature and NaOH concentration. The diffraction peaks show broadening and a slight shift towards lower Bragg angle ($2{\theta}$) which represents the reduction in crystallite size as well as presence of uniform strain. The FTIR spectra of the extract show transmittance peak fingerprint of Zn-O bond and presence of bioactive molecules These NPs exhibit inhibition greater than 50% for SCC-40, SK-MEL-2 and SCC-29B cell lines and more than 50% cell kill for SCC-29B cells at concentrations < $80{\mu}g/ml$. Nanoparticles with smallest size have shown better anti-cancer activity and peculiar cell-selectivity. The combination of extracts of these plants with ZnO NPs can be used in targeted drug delivery as an effective anti-cancer agent, a potential application in cancer treatment.

Antitumor activity of Trichosanthes kirilowii

  • Ryu, Shi-Yong;Lee, Seung-Ho;Lee, Sang-Un;Lee, Chong-Ock;No, Zaesung;Ahn, Jong-Woong
    • Archives of Pharmacal Research
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    • v.17 no.5
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    • pp.348-353
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    • 1994
  • The activity fractionation upon the MeOH extract of the root of trichosanthes kirilowii led to the isolation of eight cucurbitane tritepense namely cucurbitacin .betha. (I), isocucurbitacin .betha.(II), cucurbitacin D(III), isocucurbitacin D(IV), 3-epi-isocucurbitacin .betha(V), dihydrocucurbitain .betha. (VI), dihydroisocucurgbitachin .betha. (VII) and dihydrocucurbitacin E (VIII), as active principles. All isolates were shown to exhibit significant cytotoxicity against cultured human tumor cells, including A-549, Sk-OV-3, Sk-MEL-2, XF-498 and HCT 15, with an exceptionally high potency.

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