• Journal of Microbiology and Biotechnology
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• v.30 no.3
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• pp.469-475
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• 2020

During meiosis I, programmed DNA double-strand breaks (DSBs) occur to promote chromosome pairing and recombination between homologs. In Saccharomyces cerevisiae, Mec1 and Tel1, the orthologs of human ATR and ATM, respectively, regulate events upstream of the cell cycle checkpoint to initiate DNA repair. Tel1^ATM and Mec1^ATR are required for phosphorylating various meiotic proteins during recombination. This study aimed to investigate the role of Tel1^ATM and Mec1^ATR in meiotic prophase via physical analysis of recombination. Tel1^ATM cooperated with Mec1^ATR to mediate DSB-to-single end invasion transition, but negatively regulated DSB formation. Furthermore, Mec1^ATR was required for the formation of interhomolog joint molecules from early prophase, thus establishing a recombination partner choice. Moreover, Mec1^ATR specifically promoted crossover-fated DSB repair. Together, these results suggest that Tel1^ATM and Mec1^ATR function redundantly or independently in all post-DSB stages.

• The Transactions of the Korea Information Processing Society
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• v.7 no.6
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• pp.1844-1851
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• 2000

In this paper, a metamorphosed genetic algorithm based on the meiosis for human's chromosome is presented. In the algorithm, chromosomes in an individual are divided in half and in the other are divided into other rate. By our definition, they are composed of gametes with X-type chromosomes or Y-type chromosomes or especially M(mutation)-type chromosomes. When tow gametes among them are randomly selected and recombined, the new individual is correspondingly generated. Without reducing the searching space significantly, the global solution can be readily searched by new generated individual. The performance of he presented algorithm is examined and evaluated through proper simulation using test functions.

• Development and Reproduction
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• v.22 no.1
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• pp.65-72
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• 2018

Cyclic AMP-response element binding protein zhangfei (CREBZF), a member of ATF/CREB (activating transcription factor/ cAMP response element binding protein) family, regulates numerous cellular functions and development of cells by interacting transcription factors. This study discovered the expression pattern of CREBZF in seminiferous tubule of testes during the postnatal development of mice. In testis, CREBZF mRNA expression was the highest among other organs. Immunofluorescence analyses showed that the CREBZF was specifically expressed on spermatocyte but not in spermatogonia and Sertoli cells in seminiferous epithelium of mouse testis. Semi-quantitative polymerase chain reaction (PCR) analysis showed that CREBZF transcript level was significantly elevated during postnatal development of mouse testis. Confocal imaging analysis indicated that the protein expression of CREBZF in seminiferous tubule remained low until postnatal day (PD) 14, and was dramatically increased in PD 21. Interestingly, only one type of the spermatocyte expressed CREBZF specifically among SCP3-positive spermatocytes. Taken together, these results suggest that CREBZF may be novel putative marker of the spermatocyte and regulate meiosis during postnatal development of mice.

• Proceedings of the KIEE Conference
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• 1993.07b
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• pp.923-928
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• 1993

Genetic algorithms are powerful optimization methods based on the mechanism of natural genetics and natural selection. Genetic algorithms reduce chance of searching local optima unlike most conventional search algorithms and especially show good performances in complex nonlinear optimization problems because they do not require any information except objective function value. This paper presents a new model based on sexual reproduction in nature. In the proposed Sexual Reproduction model(SR model), individuals consist of the diploid of chromosomes, which are artificially coded as binary string in computer program. The meiosis is modeled to produce the sexual cell(gamete). In the artificial meiosis, crossover between homologous chromosomes plays an essential role for exchanging genetic informations. We apply proposed SR model to optimization of the design parameters of Single-sided Linear Induction Motor(SLIM). Sequential Unconstrained Minimization Technique(SUMT) is used to transform the nonlinear optimization problem with many constraints of SLIM to a simple unconstrained problem, We perform optimal design of SLIM available to FA conveyer systems and discuss its results.

• Journal of Animal Science and Technology
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• v.64 no.4
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• pp.654-670
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• 2022

Spermatogenesis and testis development are highly structured physiological processes responsible for post-pubertal fertility in stallions. Spermatogenesis comprises spermatocytogenesis, meiosis, and spermiogenesis. Although germ cell degeneration is a continuous process, its effects are more pronounced during spermatocytogenesis and meiosis. The productivity and efficiency of spermatogenesis are directly linked to pubertal development, degenerated germ cell populations, aging, nutrition, and season of the year in stallions. The multiplex interplay of germ cells with somatic cells, endocrine and paracrine factors, growth factors, and signaling molecules contributes to the regulation of spermatogenesis. A cell-tocell communication within the testes of these factors is a fundamental requirement of normal spermatogenesis. A noteworthy development has been made recently on discovering the effects of different somatic cells including Leydig, Sertoli, and peritubular myoid cells on manipulation the fate of spermatogonial stem cells. In this review, we discuss the self-renewal, differentiation, and apoptotic roles of somatic cells and the relationship between somatic and germ cells during normal spermatogenesis. We also summarize the roles of different growth factors, their paracrine/endocrine/autocrine pathways, and the different cytokines associated with spermatogenesis. Furthermore, we highlight important matters for further studies on the regulation of spermatogenesis. This review presents an insight into the mechanism of spermatogenesis, and helpful in developing better understanding of the functions of somatic cells, particularly in stallions and would offer new research goals for developing curative techniques to address infertility/subfertility in stallions.

• Journal of The Korean Association For Science Education
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• v.31 no.5
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• pp.680-693
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• 2011

In conceptual ecology, a concept does not exist independently but occupies an ecological niche in ecological environment. Among many biological concepts, genetic concepts are connected to several units including the genetics unit, and within the genetics unit the concepts of sub-areas are highly dependent on one another. For this reason, we analyzed conceptual diversity and conceptual proximity of genetic concepts through the ecological niche approach. For this purpose, we surveyed 995 9th graders. The areas covered in the survey were four genetic concepts: gene, chromosome, mitosis, and meiosis. The questionnaire presented biological concepts or terms related to each area, and the respondent marked the relevance between the presented biological concepts or terms and each area on a scale of 1~30 points. With 9th grade students, we analyzed the change of genetic concepts through class by the ecological niche approach. Through class, the total number of concepts increased in all of the areas, and the increase was smallest in the area of meiosis followed by mitosis, chromosome and gene. Relative density decreased with increases in the number of concepts. The conceptual diversity index also increased through class in all of the areas, and the increase was smallest in the area of meiosis followed by mitosis, chromosome and gene. In addition, difference in the relative density of concepts was reduced after class, and difference in the score of relevance was also reduced and consequently similarity among concepts increased. From these results were drawn conclusions as follows: First, through class, the conceptual diversity of genetic concepts increased. Second, through class, the conceptual proximity of genetic concepts increased.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.6
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• pp.402-408
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• 2002

Rice cultivar 'Janganbyeo' was cultivated by irrigating the saline waters of high salinity (3.0%) and medium saliniy (1.5%) for 4 days, and low salinity (0.5%) for 30 days at tillering, early meiosis and heading stage. Leaf injury due to salinity was most severe at tillering stage in 1999, but at heading stage in 2000. Heading date was delayed by 1 to 5 days by treatment of saline waters only at tillering stage. Culm length and panicle length were most severely shortened by treatment at early meiosis stage. Yield and yield components except for panicle number were decreased most by high salinity treatment regardless of growth stages. In particular, ripening ratio and grain weight among the yield components were decreased most conspicuously by the saline water treatment regardless of salinity and growth stage. Regarding grain weight grain-filling rate and duration, there is no remarked difference among the concentrations and treatment durations of saline water at tillering stage. However, their reductions were very different among the concentrations and treatment durations of saline water at early meiosis stage, being greatest when treated with high salinity for 4 days and followed by low salinity for 30 days. Also their reductions were very severe only when treated with high salinity for 4 days at heading stage.

• Korean Journal of Plant Tissue Culture
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• v.25 no.6
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• pp.453-475
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• 1998

During the 100 years since the initial discovery of meiotic phenomenon many brilliant aspects have been elucidated, but further researches based on light microscopy alone as an experimental tool have been found to have some limits and shortcomings. By the use of electron microscopy and armed with the advanced knowledges on modern genetics and biochemistry it has been possible to applu molecular technology in gaining information on the detailed aspects of meiosis. As synapsis takes place, a three-layered proteinous structure called the synatonemal complex starts to form in the space between the homologous chromosomes. To be more precise, it begins to form along the paired chromosomes early in the prophase I of meiotic division. The mechanism that leads to precise point-by-point pairing between homologous chromocomes division. The mechamism that leads to precise point-by-point pairing between homologous chromosomes remains to be ascertained. Several items of information, however, suggest that chromsome alignment leading to synapsis may be mediated somehow by the nuclear membrane. Pachytene bivalents in eukaryotes are firmly attached to the inner niclear membrane at both termini. This attached begins with unpaired leptotene chromosomes that already have developed a lateral element. Once attached, the loptotene chromosomes begin to synapse. A number of different models have been proposed to account for genetic recombination via exchange between DNA strands following their breakage and subsequent reunion in new arrangement. One of the models accounting for molecular recombination leading to chromatid exchange and chiasma formation was first proposed in 1964 by Holliday, and 30 years later still a modified version of his model is favored. Nicks are made by endomuclease at corresponding sites on one strant of each DNA duplex in nonsister chromatid of a bivalent during prophase 1 of meiosis. The nicked strands loop-out and two strands reassociate into an exchanged arrangement, which is sealed by ligase. The remaining intact strand of each duplex is nicked at a site opposite the cross-over, and the exposed ends are digested by exonuclease action. Considerable progress has been made in recent years in the effort to define the molecular and organization features of the centromere region in the yeast chromosome. Centromere core region of the DNA duplex is flanked by 15 densely packed nucleosomes on ons side and by 3 packed nucleosomes on the other side, that is, 2000 bp on one side and 400 400 bp in the other side. All the telomeres of a given species share a common DNA sequence. Two ends of each chromosome are virtually identical. At the end of each chromosome there exist two kinds of DNA sequence" simple telpmeric sequences and telpmere-associated sequencies. Various studies of telomere replication, function, and behabior are now in progress, all greatly aided by molecular methods. During nuclear division in mitosis as well as in meiosis, the nucleili disappear by the time of metaphase and reappear during nuclear reorganizations in telophase. When telophase begins, small nucleoli form at the NOR of each nucleolar-organizing chromosome, enlarge, and fuse to form one or more large nucleoli. Nucleolus is a special structure attached top a specific nucleolar-organizing region located at a specific site of a particular chromosome. The nucleolus is a vertical factory for the synthesis of rRNAs and the assenbly of ribosome subunit precursors.sors.

• Applied Microscopy
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• v.33 no.2
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• pp.105-116
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• 2003

In Vitro fertilization of U. unicinctus eggs observed by immunofluorescence and electron microscopes revealed an overview of the meiotic pattern of the tide animals. The eggs have been fertilized early at germinal vesicle stage, followed by germinal vesicle break down (GVBD), but pre-mitotic aster like structure could not be resolved by the methods employed in this work. The meiotic features, such as rotation-shift movement of spindle fibers, behavior of spermatozoonmonaster in the egg cytoplasm and active spindle fiber of the 1st polar body, have been observed. The antitubulin-FITC fluorescence show the 2nd meiotic apparatus appeared firstly parallel to the tangential line of the oolemma, proceeding the meiosis, its bipolarity is rotated and shifted towards the oolemma. The polar bodysite of the oolemma was not amorphous, but active in a sense of anti-tubulin-FITC reactions during the extrusions of the polar bodies. The immunofluorescence reactions of the spermatozoon centriole appeared at a later stage of the 2nd meiosis. During the time periods, the fertilized spermatozoon resided in the egg cytoplasm. Activating the centrioles, spermatozoon approaches towards the chromosomal materials of the 2nd oocyte. This suggests that spermatozoon centrioles initiate and play a roll to fuse male and female pronuclei.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.24 no.1
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• pp.30-36
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• 1979

The fertilizations of cv Palkweng, Noupoung, and TN 1 which were crossed artificially at 2$0^{\circ}C$ and 3$0^{\circ}C$ were examined. The meiosis and the rate of maturity of the above 3 cultivars, Iri #326, Milyang #29, Suwon #264, and 3 others which were transplated at different times in paddy field were examined, and the results of which were as follows. 1. Palkweng at 3$0^{\circ}C$ was fertilized within 1.5~4.0 hours ,after pollination and at 20\circ C was done within 2.8~4.5 hours after pollination. 2. Noupoung and TNI at 3$0^{\circ}C$ were fertilized within 2.0~5.5 hours after pollination and at 2$0^{\circ}C$ were done within 3.0~6.0 hours after pollination. 3. The rates of fertilization of the cultivated rice plants at 2$0^{\circ}C$ were dropped in general. TN 1 at 2$0^{\circ}C$ was often fertilized abnormally. 4. In the development of embryo, Palkweng was the earliest at 3$0^{\circ}C$ and TN 1 was the earliest at 2$0^{\circ}C$. 5. Although the time of transplant was late, the pollens of Palkweng was normal because the meiosis of that was normal. but the pollens of TNI was sterile in general because of the abnormal meiosis.