• Journal of Microbiology and Biotechnology
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• v.23 no.9
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• pp.1229-1243
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• 2013

In this paper, an air isolate (NITT6L) has been screened based on hemolytic activity, emulsification activity, drop collapsing test, and oil displacement test, as well as lipase activity. It was found that strain NITT6L was able to reduce the surface tension of the medium from 61.5 to 39.83 mN/m and could form stable emulsions with tested vegetable oils. Morphological, biochemical, 16S rRNA sequencing analyses, and fatty acid methyl ester analysis using gas chromatography confirmed that the air isolate under study was Pseudomonas aeruginosa. Characterization of the biosurfactant using agar double diffusion assay revealed that the biosurfactant was anionic in nature, and CTAB-methylene blue assay and Molisch test revealed its glycolipid nature. The FT-IR spectrum confirmed that the crude biosurfactant was a rhamnolipid. Using unoptimized medium containing sucrose as the carbon source, the isolate was found to produce 0.3 mg/ml of rhamnolipid in batch cultivation (shake flask) at $37^{\circ}C$ and pH 7. Optimization of the medium components was carried out using design of experiments and the yield of rhamnolipid has been enhanced to 4.6 mg/ml in 72 h of fermentation.

• Wind and Structures
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• v.13 no.5
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• pp.433-449
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• 2010

Wind tunnel model tests were conducted for a residential apartment block located within the complex terrain of The Hong Kong University of Science and Technology (HKUST). The test building is typical of medium-rise residential buildings in Hong Kong. The model study was conducted using modelling techniques and assumptions that are commonly used to predict design wind loads and pressures for buildings sited in regions of significant topography. Results for the building model with and without the surrounding topography were compared to investigate the effects of far-field and near-field topography on wind characteristics at the test building site and wind-induced external pressure coefficients at key locations on the building facade. The study also compared the wind tunnel test results to topographic multipliers and external pressure coefficients determined from nine international design standards. Differences between the external pressure coefficients stipulated in the various standards will be exacerbated when they are combined with the respective topographic multipliers.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.46 no.1
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• pp.12-16
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• 2001

In order to develop near isogenic lines (NILs) the heterozygous rice plants for alkali digestibility value(ADV) were selected and tested in every generation from $F_5$to $F_9$ of a cross, Seratus malam/Suwon 345. Finally several sets of NILs, which were six low ADV lines and four medium-high ADV lines, were selected among $F_{10}$ lines. No differences of the plant growth characteristics, amylose content and protein content of rice grain were found between low and medium-high ADV lines. Rice flour of low ADV lines showed longer gel length in gel consistency test than medium-high ADV lines, and also showed different gelatinization characteristics measured by rapid visco analyzer compared with medium-high ADV lines. The result of genetic analysis indicated that ADV-NILs developed were differed in a pair of major gene controlling ADV, and low ADV was dominant over medium-high ADV.

• Biomedical Science Letters
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• v.29 no.4
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• pp.249-255
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• 2023

Transporting clinical samples for microbiological testing requires a proper transport medium that guarantees the survival of microorganisms. Therefore, the aim of the study was to determine the ability of Amies Transport Medium (ATM) to maintain the viability of microorganisms in clinical specimens and its suitability as a transport medium for microbiological testing. This study evaluated the performance of swab provided by KS Co., Ltd. for three groups of bacteria comprising aerobic and facultative anaerobic bacteria, anaerobic bacteria, and fastidious bacteria, according to the Clinical and Laboratory Standard Institute (CLSI) 8.11.2. The ATM stability test was conducted by dividing the medium into two groups based on the product expiration date of use. All tested media, A and B (the date of manufacture and expiration date are different) showed ≥5 CFU, and there was no significant difference in the result values of Category A and Category B with different serial numbers for each test. The results of this experiment when cross-checked with the guidelines suggest that ATM is a suitable transport medium for microbiological testing, as it maintains the viability of microorganisms and is suitable for overgrowth trials. In addition, compared to the number of CFUs at the origin, the number of CFUs did not increase by more than 1 log after storage. These results have important implications for the development of transport media that can guarantee the survival of microorganisms in clinical specimens.

• Korean Journal of Ecology and Environment
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• v.45 no.3
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• pp.271-277
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• 2012

The present study was conducted to assess the suitability of domestic natural waters as a Daphnia magna culture medium. In order to assess survival rate and reproductive output, young female daphnids (parent animals), aged less than 24 hours at the start of the test and produced in the Elendt M4 medium, were exposed to Elendt M4 medium, de-chlorinated tap water, and natural mineral water for 21 days. D. magna cultured in Elendt M4 medium (reference medium) and natural mineral water met the criteria of OECD No. 211, Daphnia magna Reproduction Test Guidelines in terms of percent adult survival, first day of reproduction, and average young production. However, the mortalities of adult daphnids observed in de-chlorinated tap water were more than 20% in two reproduction tests for 21 days. Mortality was observed on exposure days 13, 15, and 18 in de-chlorinated water. The use of D. magna is recommended in water of hardness >80 mg $CaCO_3\;L^{-1}$. However, the hardness of de-chlorinated tap water used in the present study was 50~53 mg $CaCO_3\;L^{-1}$. Therefore, it is judged that the delayed mortalities observed in de-chlorinated tap water were caused by a rapid decreased in hardness when the medium was changed from Elendt M4 to de-chlorinated tap water. When D. magna is cultured using domestic natural waters (underground water, surface water, and de-chlorinated water), the quality-control (QC) data should be maintained through a standardization for health assessment method, toxicity test method using reference chemical, test intervals of reference toxicant toxicity test, and data treatment and interpretation. In the long term, national research programs are needed for the development of test species which are representative of domestic aquatic environmental conditions among indigenous daphnids.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2004.10a
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• pp.681-685
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• 2004

A media-feeding (or media-transport) system is a key component in daily consumer systems such as printers, copiers and ATM's. The role of the media-transport system is to feed a medium, which is usually in the form of a thin film, to the main process in a uniform and repeatable manner. Even small slippage between the media and the feeding rollers could significantly degrade the performance of the entire system. The slippage between the medium and the feeding rollers is determined by many parameters which include the friction coefficient between the feeding rollers and the medium material, the angular velocity of the feeding rollers, and the normal force applied by feeding rollers on the medium. This paper investigates the effect of the normal force and the angular velocity of feeding rollers on the slippage of the medium. Authors have constructed a test bed for experiments, which consists of a feeding module and various measuring devices. Using regular paper as media being fed, the authors experimentally measured the slippage of the medium under various normal forces and angular velocities of driving feeding roller. Also the authors developed a novel two-dimensional simulation model for the media-transport system. The paper medium is modeled as a set of multiple rigid bodies interconnected by revolute joints and rotational springs and dampers. Simulations were executed using a multi-body dynamic analysis tool called RecurDy $n^{ⓡ}$. The slippage obtained by the simulation is compared to experimental results.ults.

• Journal of Mushroom
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• v.10 no.4
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• pp.167-173
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• 2012

This study was carried out to test the recycling post-harvest medium from other mushroom bottle cultivation as a secondary medium of the oyster mushroom. In the post-harvest medium from winter mushroom and king oyster mushroom cultivation, oyster mushroom varieties in Chunchu-2ho and Manchuri fruit bodies yields compared with control group tend to be low. After recycling the post-harvest medium, it was replaced by basal medium up to 50%, of which the fruit bodies with stable yield increase from 10% to 30% were increased.

• Journal of the Semiconductor & Display Technology
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• v.16 no.3
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• pp.71-77
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• 2017

As the automobile industry has shifted to software, the Automotive SPICE standard has been established to ensure efficient product development process and quality. In the assessment model, the HIS Scope is the minimum standard for small and medium automotive electric companies to meet OEM requirements. However, in order to achieve the HIS Scope, the output of each process stage that meets the verification criteria of Automotive SPICE must be created. In particular, the test phase takes a lot of resources, which is a big burden for small and medium-sized companies. In this paper, we propose a methodology for creating test cases of software integration test phase based on UML sequence diagram, which is a software design phase of Automotive SPICE HIS Scope, by applying behavior model based testing method. We also propose a tool chain for automating the creation process. This will reduce the resources required to create a test case.

• The Korean Journal of Pain
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• v.18 no.2
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• pp.124-132
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• 2005

Background: This study was performed to evaluate the dose-related effects of naloxone on morphine analgesia in the rat formalin test, and observe the correlation of pain behavior and spinal c-fos expression induced by a formalin injection. Methods: Fifty rats were divided into five groups; control, morphine (morphine pre-treated, intra-peritoneal injection of 0.1 mg of morphine 5 min prior to formalin injection), and three naloxone groups, which were divided according to the administered dose-ratio of naloxone to morphine 20 : 1 ($5{\mu}g$), 10 : 1 ($10{\mu}g$), and 1 : 1 ($100{\mu}g$) representing the low-, medium-, and high-dose naloxone groups, respectively, were injected intra-peritoneally 16 min after a formalin. A fifty ul of 5% formalin was injected into the right hind paw. All rats were observed for their pain behavior according to the number of flinches during phases 1 (2-3, 5-6 min) and 2 (1 min per every 5 min from 10 to 61 min). The spinal c-fos expression was quantitatively analyzed at 1 and 2 hours after the formalin injection using a real-time PCR. Results: The morphine pre-treated (morphine and three naloxone) groups during phase 1, and the morphine, low- and medium-dose naloxone groups during phase 2, showed significantly less flinches compared to those of the control (P < 0.05). In the three naloxone groups, the numbers of flinches were transiently reduced following the naloxone injection in the low- and medium-dose groups compared to those of the morphine group (P < 0.05). The duration of the reduced flinches was longer in the medium-dose group (P < 0.05). The high-dose group revealed immediate increases in flinches immediately after the naloxone injection compared to those of the morphine, low- and medium-dose groups (P < 0.05 for each). The spinal c-fos expression showed no significant patterns between the experimental groups. Conclusions: Our data suggest that relatively low-dose naloxone (1/20 to 1/10 dose-ratio of morphine) transiently potentiates morphine analgesia; whereas, high-dose (equal dose-ratio of morphine) reverses the analgesia, and the spinal c-fos expression does not always correlate with pain behavior in the rat formalin test.

• Proceedings of the KSR Conference
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• 2011.05a
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• pp.1214-1221
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• 2011

It is an well-known fact that dynamic properties should be considered in design and maintenance of civil structures undergoing dynamic force such as rail track. For designing of the rail tack structures, dynamic properties of track bed soil such as shear modulus (G) and damping coefficients(D) obtained in small to medium range of shear strain must be known. In general, small size sample of D=5 cm and H=10cm has been used mostly for test convenience. However, ratio of largest particle diameter of the soil to sample diameter is very important and affects to the values of dynamic soil properties in track bed. In this study, an RC/TS test apparatus was built and was run for testing a medium size soil sample that can handle with compacted soil sample up to 10 cm diameter and 20 cm height.